Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-11

AUTHORS

Danwei Huangfu, Kenji Osafune, René Maehr, Wenjun Guo, Astrid Eijkelenboom, Shuibing Chen, Whitney Muhlestein, Douglas A Melton

ABSTRACT

Ectopic expression of defined sets of genetic factors can reprogram somatic cells to induced pluripotent stem (iPS) cells that closely resemble embryonic stem (ES) cells. The low efficiency with which iPS cells are derived hinders studies on the molecular mechanism of reprogramming, and integration of viral transgenes, in particular the oncogenes c-Myc and Klf4, may handicap this method for human therapeutic applications. Here we report that valproic acid (VPA), a histone deacetylase inhibitor, enables reprogramming of primary human fibroblasts with only two factors, Oct4 and Sox2, without the need for the oncogenes c-Myc or Klf4. The two factor-induced human iPS cells resemble human ES cells in pluripotency, global gene expression profiles and epigenetic states. These results support the possibility of reprogramming through purely chemical means, which would make therapeutic use of reprogrammed cells safer and more practical. More... »

PAGES

1269-1275

References to SciGraph publications

  • 2007-06. A ROCK inhibitor permits survival of dissociated human embryonic stem cells in NATURE BIOTECHNOLOGY
  • 2007-07. Stem cells: The magic brew in NATURE
  • 1997-02. Viable offspring derived from fetal and adult mammalian cells in NATURE
  • 2008-07. Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds in NATURE BIOTECHNOLOGY
  • 1958-07. Sexually Mature Individuals of Xenopus laevis from the Transplantation of Single Somatic Nuclei in NATURE
  • 2008-07. Pluripotent stem cells induced from adult neural stem cells by reprogramming with two factors in NATURE
  • 2008-07-03. Dissecting direct reprogramming through integrative genomic analysis in NATURE
  • 2007-11. Producing primate embryonic stem cells by somatic cell nuclear transfer in NATURE
  • 2008-01. Reprogramming of human somatic cells to pluripotency with defined factors in NATURE
  • 2007-10. Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells in NATURE BIOTECHNOLOGY
  • 2008-03. Marked differences in differentiation propensity among human embryonic stem cell lines in NATURE BIOTECHNOLOGY
  • 2003-04. Identification and isolation of multipotential neural progenitor cells from the subcortical white matter of the adult human brain in NATURE MEDICINE
  • 1998-07. Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei in NATURE
  • 2005-12. Efficient differentiation of human embryonic stem cells to definitive endoderm in NATURE BIOTECHNOLOGY
  • 2007-07. In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state in NATURE
  • 2006-11. Induced pluripotency and cellular alchemy in NATURE BIOTECHNOLOGY
  • 2008-01. Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts in NATURE BIOTECHNOLOGY
  • 2007-07. Generation of germline-competent induced pluripotent stem cells in NATURE
  • Journal

    TITLE

    Nature Biotechnology

    ISSUE

    11

    VOLUME

    26

    Related Patents

  • Generation Of Induced Pluripotent Stem Cells From Cord Blood
  • Dental Stem Cell Reprogramming
  • Method For Generating Induced Pluripotent Stem Cells From Keratinocytes Derived From Plucked Hair Follicles
  • A Non-Viral System For The Generation Of Induced Pluripotent Stem (Ips) Cells
  • Induced Pluripotent Stem Cells Produced With Oct3/4, Klf4 And Sox2
  • Induction Of Pluripotent Cells
  • Modified Polynucleotides For The Production Of Secreted Proteins
  • Generation Of Induced Pluripotent Stem Cells From Cord Blood
  • Methods For Producing Enucleated Erythroid Cells Derived From Pluripotent Stem Cells
  • Differentiating Induced Pluripotent Stem Cells Into Glucose-Responsive, Insulin-Secreting Progeny
  • Nuclear Reprogramming Factor Comprising Mirna And A Protein Factor
  • Engineered Nucleic Acids Encoding A Modified Erythropoietin And Their Expression
  • Generating Induced Pluripotent Stem Cells And Progenitor Cells From Fibroblasts
  • Reprogramming Cells
  • Method For Inducing Cardiac Differentiation Of Pluripotent Stem Cell
  • Cardiomyocytes From Induced Pluripotent Stem Cells From Patients And Methods Of Use Thereof
  • Protein-Induced Pluripotent Cell Technology And Uses Thereof
  • Generation And Maintenance Of Stem Cells
  • Novel Method For Rapid And Efficient Generation Of Neuroectoderm Cells And Peripheral Neurons From Pluripotent Stem Cells
  • Modified Polynucleotides For The Production Of Biologics And Proteins Associated With Human Disease
  • Modified Polynucleotides For Treating Galactosylceramidase Protein Deficiency
  • Method Of Reprogramming A Cell
  • Modified Polynucleotides Encoding Granulysin
  • Generation And Use Of Pluripotent Stem Cells
  • Generating Ips Cells By Protein Transduction Of Recombinant Potency-Determining Factors
  • Generation Of Induced Pluripotent Stem Cells From Cord Blood
  • Improved Method For Stem Cell Differentiation In Vivo By Delivery Of Morphogenes With Mesoporous Silica And Corresponding Pharmaceutical Active Ingredients.
  • A Method Of Preparing Isolated Cells And Uses
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Rna Preparations Comprising Purified Modified Rna For Reprogramming Cells
  • Modified Polynucleotides Encoding Cited4
  • Modified Polynucleotides For The Production Of Nuclear Proteins
  • Modified Polynucleotides Encoding Septin-4
  • Efficient Method For Nuclear Reprogramming
  • Generation Of Induced Pluripotent Stem Cells From Cord Blood
  • Generation Of Patient-Specific Differentiated Cell Types By Epigenetic Induction
  • Compositions And Methods For Reprogramming Eukaryotic Cells
  • A Protein-Induced Pluripotent Cell Technology Uses Thereof
  • Combined Chemical And Genetic Approaches For Generation Of Induced Pluripotent Stem Cells
  • Modified Polynucleotides For Treating Protein Deficiency
  • Somatic Cell Reprogramming By Retroviral Vectors Encoding Oct3/4. Klf4, C-Myc And Sox2
  • Vitronectin-Derived Cell Culture Substrate And Uses Thereof
  • Generation And Maintenance Of Stem Cells
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Modified Polynucleotides Encoding Apoptosis Inducing Factor 1
  • Induction Of Pluripotent Cells
  • Delivery And Formulation Of Engineered Nucleic Acids
  • Cell Compositions Derived From Dedifferentiated Reprogrammed Cells
  • Methods And Platforms For Drug Discovery Using Induced Pluripotent Stem Cells
  • Rna Preparations Comprising Purified Modified Rna For Reprogramming Cells
  • Transcriptome Transfer Produces Cellular Phenotype Conversion
  • Modified Polynucleotides For The Production Of Proteins Associated With Human Disease
  • Reprogramming Of Cells To A New Fate
  • Delivery And Formulation Of Engineered Nucleic Acids
  • Enhancers Of Induced Pluripotent Stem Cell Reprogramming
  • Method For Increasing The Efficiency Of Inducing Pluripotent Stem Cells
  • Production Of Reprogrammed Pluripotent Cells
  • Polycistronic Vector For Human Induced Pluripotent Stem Cell Production
  • Epigenetic Induction Of Human Patient-Specific Self-Propagating Dopaminergic Neuron Cell
  • Generation Of Patient-Specific Differentiated Cell Types By Epigenetic Induction
  • Method For Promoting Differentiation Of Pluripotent Stem Cells Into Cardiac Muscle Cells
  • Hemangio Colony Forming Cells And Non-Engrafting Hemangio Cells
  • Rna Preparations Comprising Purified Modified Rna For Reprogramming Cells
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Modified Polynucleotides Encoding Copper Metabolism (Murr1) Domain Containing 1
  • Method Of Treating Heart Tissue Using Induced Pluripotent Stem Cells
  • Cell Compositions Derived From Dedifferentiated Reprogrammed Cells
  • Human Blood-Brain Endothelial Cells Derived From Pluripotent Stem Cells And Blood-Brain Barrier Model Thereof
  • Bacterial Mediated Delivery Of Nuclear Protein Into Pluripotent And Differentiated Cells
  • Induced Pluripotent Stem Cells Produced Using Reprogramming Factors And A Rho Kinase Inhibitor Or A Histone Deacetylase Inhibitor
  • Making And Using In Vitro-Synthesized Ssrna For Introducing Into Mammalian Cells To Induce A Biological Or Biochemical Effect
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Formulation And Delivery Of Plga Microspheres
  • Vitronectin-Derived Cell Culture Substrate And Uses Thereof
  • Compositions And Methods For Reprogramming Eukaryotic Cells
  • Compositions And Methods Of Altering Cholesterol Levels
  • Modified Polynucleotides Encoding Basic Helix-Loop-Helix Family Member E41
  • Method For Increasing The Efficiency Of Inducing Pluripotent Stem Cells
  • Modified Polynucleotides For The Production Of Proteins
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Conversion Of Somatic Cells To Induced Reprogrammed Neural Stem Cells (Irnscs)
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Providing Ipscs To A Customer
  • Method For Production Of Mast Cells From Stem Cells
  • Modified Polynucleotides For The Production Of Oncology-Related Proteins And Peptides
  • Modified Polynucleotides For The Production Of G-Csf
  • Nuclear Reprogramming Factor
  • Methods, Systems And Compositions Relating To Cell Conversion Via Protein-Induced In-Vivo Cell Reprogramming
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Hemangio-Colony Forming Cells
  • Terminally Modified Rna
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Reprogramming A Cell By Activation Of The Endogenous Transcription Factor Network
  • Novel Nuclear Reprogramming Substance
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Modified Polynucleotides For The Production Of Cosmetic Proteins And Peptides
  • Modified Polynucleotides Encoding Siah E3 Ubiquitin Protein Ligase 1
  • Making And Using In Vitro-Synthesized Ssrna For Introducing Into Mammalian Cells To Induce A Biological Or Biochemical Effect
  • Nuclear Reprogrammed Cells Generated By Introduction Of A Histone H2aa Or Th2a Gene, A Histone H2ba Or Th2b Gene, Or A Phosphorylation-Mimic Of Histone Chaperon Npm2 Gene, An Oct Family Gene And A Klf Family Gene Into A Mammalian Somatic Cell
  • Dlin-Kc2-Dma Lipid Nanoparticle Delivery Of Modified Polynucleotides
  • Modified Polynucleotides Encoding Siah E3 Ubiquitin Protein Ligase 1
  • Programming And Reprogramming Of Cells
  • Modified Polynucleotides Encoding Hepatitis A Virus Cellular Receptor 2
  • Reprogramming Cells
  • Dlin-Kc2-Dma Lipid Nanoparticle Delivery Of Modified Polynucleotides
  • Multipotent/Pluripotent Cells And Methods
  • Method For Generating Pancreatic Hormone-Producing Cells
  • Modified Polynucleotides For The Production Of Secreted Proteins
  • Tgf-Beta Receptor Inhibitors To Enhance Direct Reprogramming
  • Generation And Use Of Pluripotent Stem Cells
  • Oct3/4, Klf4, C-Myc And Sox2 Produce Induced Pluripotent Stem Cells
  • Rna Preparations Comprising Purified Modified Rna For Reprogramming Cells
  • Modified Polynucleotides For The Production Of Proteins
  • Method For Producing A Protein Of Interest In A Primate
  • Rna Preparations Comprising Purified Modified Rna For Reprogramming Cells
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Large Scale Generation Of Functional Megakaryocytes And Platelets From Human Embryonic Stem Cells Under Stromal-Free Conditions
  • Cell Compositions Derived From Dedifferentiated Reprogrammed Cells
  • Cell Compositions Derived From Dedifferentiated Reprogrammed Cells
  • Combined Chemical And Genetic Approaches For Generation Of Induced Pluripotent Stem Cells
  • Cell Culture Platform For Single Cell Sorting And Enhanced Reprogramming Of Ipscs
  • Modified Polynucleotides For The Production Of Cytoplasmic And Cytoskeletal Proteins
  • Human Pluripotent Stem Cells Induced From Undifferentiated Stem Cells Derived From A Human Postnatal Tissue
  • Modified Polynucleotides For The Production Of Cytoplasmic And Cytoskeletal Proteins
  • Modified Polynucleotides Encoding Aquaporin-5
  • Modified Polynucleotides For The Production Of Proteins Associated With Blood And Lymphatic Disorders
  • Transcriptome Transfer Produces Cellular Phenotype Conversion
  • Multipotent/Pluripotent Cells And Methods
  • Modified Polynucleotides Encoding Aryl Hydrocarbon Receptor Nuclear Translocator
  • Enhancers Of Induced Pluripotent Stem Cell Reprogramming
  • Modified Polynucleotides For The Production Of Proteins Associated With Blood And Lymphatic Disorders
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Method For Promoting Differentiation Of Pluripotent Stem Cells Into Cardiac Muscle Cells
  • Rna Preparations Comprising Purified Modified Rna For Reprogramming Cells
  • Split Dose Administration
  • Reprogramming Of Cells To A New Fate
  • Modified Polynucleotides For The Production Of Biologics And Proteins Associated With Human Disease
  • Formulation And Delivery Of Plga Microspheres
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nbt.1502

    DOI

    http://dx.doi.org/10.1038/nbt.1502

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1051466228

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/18849973


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Biotechnology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Differentiation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cells, Cultured", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cellular Reprogramming", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Culture Media", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Embryonic Stem Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Epigenesis, Genetic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Fibroblasts", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression Profiling", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Octamer Transcription Factor-3", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Pluripotent Stem Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "SOXB1 Transcription Factors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Valproic Acid", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Harvard University", 
              "id": "https://www.grid.ac/institutes/grid.38142.3c", 
              "name": [
                "Department of Stem Cell and Regenerative Biology, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts 02138, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Huangfu", 
            "givenName": "Danwei", 
            "id": "sg:person.01014266246.80", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01014266246.80"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Japan Science and Technology Agency", 
              "id": "https://www.grid.ac/institutes/grid.419082.6", 
              "name": [
                "Department of Stem Cell and Regenerative Biology, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts 02138, USA.", 
                "ICORP Organ Regeneration Project, Japan Science and Technology Agency (JST), 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Osafune", 
            "givenName": "Kenji", 
            "id": "sg:person.01143207360.34", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01143207360.34"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Harvard University", 
              "id": "https://www.grid.ac/institutes/grid.38142.3c", 
              "name": [
                "Department of Stem Cell and Regenerative Biology, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts 02138, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Maehr", 
            "givenName": "Ren\u00e9", 
            "id": "sg:person.0744246221.84", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744246221.84"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Massachusetts Institute of Technology", 
              "id": "https://www.grid.ac/institutes/grid.116068.8", 
              "name": [
                "The Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Guo", 
            "givenName": "Wenjun", 
            "id": "sg:person.014605715475.58", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014605715475.58"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Utrecht University", 
              "id": "https://www.grid.ac/institutes/grid.5477.1", 
              "name": [
                "Department of Stem Cell and Regenerative Biology, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts 02138, USA.", 
                "Biomedical Sciences, Utrecht University, The Netherlands."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Eijkelenboom", 
            "givenName": "Astrid", 
            "id": "sg:person.01054122426.39", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01054122426.39"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Harvard University", 
              "id": "https://www.grid.ac/institutes/grid.38142.3c", 
              "name": [
                "Department of Stem Cell and Regenerative Biology, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts 02138, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chen", 
            "givenName": "Shuibing", 
            "id": "sg:person.016474257017.70", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016474257017.70"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Harvard University", 
              "id": "https://www.grid.ac/institutes/grid.38142.3c", 
              "name": [
                "Department of Stem Cell and Regenerative Biology, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts 02138, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Muhlestein", 
            "givenName": "Whitney", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Harvard University", 
              "id": "https://www.grid.ac/institutes/grid.38142.3c", 
              "name": [
                "Department of Stem Cell and Regenerative Biology, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts 02138, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Melton", 
            "givenName": "Douglas A", 
            "id": "sg:person.01164235020.37", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164235020.37"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/j.cell.2007.11.020", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000473144"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature05934", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002347899", 
              "https://doi.org/10.1038/nature05934"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1374", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002744128", 
              "https://doi.org/10.1038/nbt1374"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/28615", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005040238", 
              "https://doi.org/10.1038/28615"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/28615", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005040238", 
              "https://doi.org/10.1038/28615"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0896-6273(00)00083-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005164056"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2008.05.011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006466689"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.c400479200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007614091"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/448260a", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009764115", 
              "https://doi.org/10.1038/448260a"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2007.11.019", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010904856"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2007.08.008", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011219533"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature06534", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011992920", 
              "https://doi.org/10.1038/nature06534"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2007.12.001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012875023"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2008.06.019", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013817335"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1418", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014318073", 
              "https://doi.org/10.1038/nbt1418"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2006.07.024", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014573758"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1106-1363", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016800008", 
              "https://doi.org/10.1038/nbt1106-1363"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1106-1363", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016800008", 
              "https://doi.org/10.1038/nbt1106-1363"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature07061", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017686107", 
              "https://doi.org/10.1038/nature07061"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1091/mbc.e07-01-0029", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018776426"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature05944", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019109862", 
              "https://doi.org/10.1038/nature05944"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.med.58.071105.110848", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019541541"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1056/nejmsr040330", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022304644"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm837", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023534021", 
              "https://doi.org/10.1038/nm837"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm837", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023534021", 
              "https://doi.org/10.1038/nm837"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0711983105", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024490582"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1310", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024578139", 
              "https://doi.org/10.1038/nbt1310"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.282.5391.1145", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028898955"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2007.05.014", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029649252"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1116447", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032194044"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/182064a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036323125", 
              "https://doi.org/10.1038/182064a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0960-9822(01)00459-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037472156"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature07056", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037743725", 
              "https://doi.org/10.1038/nature07056"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1383", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039633163", 
              "https://doi.org/10.1038/nbt1383"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/385810a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043346431", 
              "https://doi.org/10.1038/385810a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature06357", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046768545", 
              "https://doi.org/10.1038/nature06357"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1151526", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048905674"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2005.08.020", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049638413"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2005.08.020", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049638413"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1335", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049953935", 
              "https://doi.org/10.1038/nbt1335"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1163", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051219192", 
              "https://doi.org/10.1038/nbt1163"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1163", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051219192", 
              "https://doi.org/10.1038/nbt1163"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/dbio.2000.9912", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053693663"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2008-11", 
        "datePublishedReg": "2008-11-01", 
        "description": "Ectopic expression of defined sets of genetic factors can reprogram somatic cells to induced pluripotent stem (iPS) cells that closely resemble embryonic stem (ES) cells. The low efficiency with which iPS cells are derived hinders studies on the molecular mechanism of reprogramming, and integration of viral transgenes, in particular the oncogenes c-Myc and Klf4, may handicap this method for human therapeutic applications. Here we report that valproic acid (VPA), a histone deacetylase inhibitor, enables reprogramming of primary human fibroblasts with only two factors, Oct4 and Sox2, without the need for the oncogenes c-Myc or Klf4. The two factor-induced human iPS cells resemble human ES cells in pluripotency, global gene expression profiles and epigenetic states. These results support the possibility of reprogramming through purely chemical means, which would make therapeutic use of reprogrammed cells safer and more practical.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nbt.1502", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1115214", 
            "issn": [
              "1087-0156", 
              "1546-1696"
            ], 
            "name": "Nature Biotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "11", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "26"
          }
        ], 
        "name": "Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2", 
        "pagination": "1269-1275", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "f529c2fcc7ff4e6effa460ac0ef98dc8a7848e24f1728df0ca2cac29d09452f2"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "18849973"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9604648"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nbt.1502"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1051466228"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nbt.1502", 
          "https://app.dimensions.ai/details/publication/pub.1051466228"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T21:25", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8687_00000426.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/articles/nbt.1502"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nbt.1502'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nbt.1502'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nbt.1502'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nbt.1502'


     

    This table displays all metadata directly associated to this object as RDF triples.

    324 TRIPLES      21 PREDICATES      83 URIs      37 LITERALS      25 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nbt.1502 schema:about N04bb348d4ae541609db6105ce2d333cd
    2 N1e781fa75def4d8b907af540461ac692
    3 N22fa760d2cec44b2a977876840baca57
    4 N3393c9df0c2e4a33a10d68ed6dc316f5
    5 N402426c0d1bb4429a63d7298b3e4c4ad
    6 N426c7deb0f2b4c65a7dc06e225a83439
    7 N5320f0ea2cdb44a3986ce896de1026c4
    8 N5d78a078a01d485aab06a47031d36f2b
    9 N667aac07ca87492d9c38349f0d4e28fe
    10 Na89862b5f7c6486b9656ff0bb5a40b8c
    11 Nc87e7bc09e0f4df7ad2c3515371e1d01
    12 Nea9278bc26bd4253912cd58612512045
    13 Nedbacf00164d4fee986debedb63bb7b3
    14 Neefd7d86a63f4283aebc5d858f7bf81f
    15 Nf13dce93b43b4fcd881191c89818ca90
    16 Nf3ffee9d8b034e2a9df6ebe64d2b8a27
    17 anzsrc-for:06
    18 anzsrc-for:0604
    19 schema:author N7bee37c587ce4514ab93e04441bd8aa7
    20 schema:citation sg:pub.10.1038/182064a0
    21 sg:pub.10.1038/28615
    22 sg:pub.10.1038/385810a0
    23 sg:pub.10.1038/448260a
    24 sg:pub.10.1038/nature05934
    25 sg:pub.10.1038/nature05944
    26 sg:pub.10.1038/nature06357
    27 sg:pub.10.1038/nature06534
    28 sg:pub.10.1038/nature07056
    29 sg:pub.10.1038/nature07061
    30 sg:pub.10.1038/nbt1106-1363
    31 sg:pub.10.1038/nbt1163
    32 sg:pub.10.1038/nbt1310
    33 sg:pub.10.1038/nbt1335
    34 sg:pub.10.1038/nbt1374
    35 sg:pub.10.1038/nbt1383
    36 sg:pub.10.1038/nbt1418
    37 sg:pub.10.1038/nm837
    38 https://doi.org/10.1006/dbio.2000.9912
    39 https://doi.org/10.1016/j.cell.2005.08.020
    40 https://doi.org/10.1016/j.cell.2006.07.024
    41 https://doi.org/10.1016/j.cell.2007.11.019
    42 https://doi.org/10.1016/j.cell.2007.11.020
    43 https://doi.org/10.1016/j.stem.2007.05.014
    44 https://doi.org/10.1016/j.stem.2007.08.008
    45 https://doi.org/10.1016/j.stem.2007.12.001
    46 https://doi.org/10.1016/j.stem.2008.05.011
    47 https://doi.org/10.1016/j.stem.2008.06.019
    48 https://doi.org/10.1016/s0896-6273(00)00083-0
    49 https://doi.org/10.1016/s0960-9822(01)00459-6
    50 https://doi.org/10.1056/nejmsr040330
    51 https://doi.org/10.1073/pnas.0711983105
    52 https://doi.org/10.1074/jbc.c400479200
    53 https://doi.org/10.1091/mbc.e07-01-0029
    54 https://doi.org/10.1126/science.1116447
    55 https://doi.org/10.1126/science.1151526
    56 https://doi.org/10.1126/science.282.5391.1145
    57 https://doi.org/10.1146/annurev.med.58.071105.110848
    58 schema:datePublished 2008-11
    59 schema:datePublishedReg 2008-11-01
    60 schema:description Ectopic expression of defined sets of genetic factors can reprogram somatic cells to induced pluripotent stem (iPS) cells that closely resemble embryonic stem (ES) cells. The low efficiency with which iPS cells are derived hinders studies on the molecular mechanism of reprogramming, and integration of viral transgenes, in particular the oncogenes c-Myc and Klf4, may handicap this method for human therapeutic applications. Here we report that valproic acid (VPA), a histone deacetylase inhibitor, enables reprogramming of primary human fibroblasts with only two factors, Oct4 and Sox2, without the need for the oncogenes c-Myc or Klf4. The two factor-induced human iPS cells resemble human ES cells in pluripotency, global gene expression profiles and epigenetic states. These results support the possibility of reprogramming through purely chemical means, which would make therapeutic use of reprogrammed cells safer and more practical.
    61 schema:genre research_article
    62 schema:inLanguage en
    63 schema:isAccessibleForFree false
    64 schema:isPartOf N5f42d0020eea4175b33ead3f763bb065
    65 N8fc526af5d4a460a8be859283d8dba7d
    66 sg:journal.1115214
    67 schema:name Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2
    68 schema:pagination 1269-1275
    69 schema:productId N16221fe1aab149028b3215a56eb96e67
    70 N28a12d9c10e543a6bf6fa964c136271f
    71 N638528c5a862400c9c89b7f6f6836056
    72 Nb1c23dfe38fc4966ab3ebaa1fec2b82c
    73 Nf9b4626fd7a34988899bb2e3bf3953e5
    74 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051466228
    75 https://doi.org/10.1038/nbt.1502
    76 schema:sdDatePublished 2019-04-10T21:25
    77 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    78 schema:sdPublisher N82bc60bff678415683052b85afe2b580
    79 schema:url http://www.nature.com/articles/nbt.1502
    80 sgo:license sg:explorer/license/
    81 sgo:sdDataset articles
    82 rdf:type schema:ScholarlyArticle
    83 N027353b0f2e7484e995233eb1346be76 rdf:first sg:person.014605715475.58
    84 rdf:rest N2ab1e9e9626f4abfa9e3677121ccbd1b
    85 N04bb348d4ae541609db6105ce2d333cd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    86 schema:name Fibroblasts
    87 rdf:type schema:DefinedTerm
    88 N16221fe1aab149028b3215a56eb96e67 schema:name dimensions_id
    89 schema:value pub.1051466228
    90 rdf:type schema:PropertyValue
    91 N16d4ba602705440e87f392659511f002 rdf:first sg:person.0744246221.84
    92 rdf:rest N027353b0f2e7484e995233eb1346be76
    93 N1e781fa75def4d8b907af540461ac692 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    94 schema:name Gene Expression Profiling
    95 rdf:type schema:DefinedTerm
    96 N22fa760d2cec44b2a977876840baca57 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    97 schema:name Cells, Cultured
    98 rdf:type schema:DefinedTerm
    99 N28a12d9c10e543a6bf6fa964c136271f schema:name doi
    100 schema:value 10.1038/nbt.1502
    101 rdf:type schema:PropertyValue
    102 N2ab1e9e9626f4abfa9e3677121ccbd1b rdf:first sg:person.01054122426.39
    103 rdf:rest N4b3c90396f7b4e46a891ba39323955d9
    104 N3393c9df0c2e4a33a10d68ed6dc316f5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    105 schema:name Embryonic Stem Cells
    106 rdf:type schema:DefinedTerm
    107 N3ed293195de54f4d914ed741487eb482 rdf:first sg:person.01164235020.37
    108 rdf:rest rdf:nil
    109 N402426c0d1bb4429a63d7298b3e4c4ad schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name Mice
    111 rdf:type schema:DefinedTerm
    112 N426c7deb0f2b4c65a7dc06e225a83439 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    113 schema:name Animals
    114 rdf:type schema:DefinedTerm
    115 N4b3c90396f7b4e46a891ba39323955d9 rdf:first sg:person.016474257017.70
    116 rdf:rest Nadcc3352032d4e26a2b38a64d02fa284
    117 N5320f0ea2cdb44a3986ce896de1026c4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    118 schema:name Biotechnology
    119 rdf:type schema:DefinedTerm
    120 N5d78a078a01d485aab06a47031d36f2b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    121 schema:name Cellular Reprogramming
    122 rdf:type schema:DefinedTerm
    123 N5f42d0020eea4175b33ead3f763bb065 schema:issueNumber 11
    124 rdf:type schema:PublicationIssue
    125 N638528c5a862400c9c89b7f6f6836056 schema:name readcube_id
    126 schema:value f529c2fcc7ff4e6effa460ac0ef98dc8a7848e24f1728df0ca2cac29d09452f2
    127 rdf:type schema:PropertyValue
    128 N667aac07ca87492d9c38349f0d4e28fe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    129 schema:name Cell Differentiation
    130 rdf:type schema:DefinedTerm
    131 N7b1fd8c1c90247e29d3d897f6354a47e schema:affiliation https://www.grid.ac/institutes/grid.38142.3c
    132 schema:familyName Muhlestein
    133 schema:givenName Whitney
    134 rdf:type schema:Person
    135 N7bee37c587ce4514ab93e04441bd8aa7 rdf:first sg:person.01014266246.80
    136 rdf:rest Nf8aba94d9acf49b2b3138726faccffcf
    137 N82bc60bff678415683052b85afe2b580 schema:name Springer Nature - SN SciGraph project
    138 rdf:type schema:Organization
    139 N8fc526af5d4a460a8be859283d8dba7d schema:volumeNumber 26
    140 rdf:type schema:PublicationVolume
    141 Na89862b5f7c6486b9656ff0bb5a40b8c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    142 schema:name Humans
    143 rdf:type schema:DefinedTerm
    144 Nadcc3352032d4e26a2b38a64d02fa284 rdf:first N7b1fd8c1c90247e29d3d897f6354a47e
    145 rdf:rest N3ed293195de54f4d914ed741487eb482
    146 Nb1c23dfe38fc4966ab3ebaa1fec2b82c schema:name pubmed_id
    147 schema:value 18849973
    148 rdf:type schema:PropertyValue
    149 Nc87e7bc09e0f4df7ad2c3515371e1d01 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    150 schema:name Octamer Transcription Factor-3
    151 rdf:type schema:DefinedTerm
    152 Nea9278bc26bd4253912cd58612512045 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    153 schema:name SOXB1 Transcription Factors
    154 rdf:type schema:DefinedTerm
    155 Nedbacf00164d4fee986debedb63bb7b3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    156 schema:name Valproic Acid
    157 rdf:type schema:DefinedTerm
    158 Neefd7d86a63f4283aebc5d858f7bf81f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    159 schema:name Culture Media
    160 rdf:type schema:DefinedTerm
    161 Nf13dce93b43b4fcd881191c89818ca90 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    162 schema:name Epigenesis, Genetic
    163 rdf:type schema:DefinedTerm
    164 Nf3ffee9d8b034e2a9df6ebe64d2b8a27 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    165 schema:name Pluripotent Stem Cells
    166 rdf:type schema:DefinedTerm
    167 Nf8aba94d9acf49b2b3138726faccffcf rdf:first sg:person.01143207360.34
    168 rdf:rest N16d4ba602705440e87f392659511f002
    169 Nf9b4626fd7a34988899bb2e3bf3953e5 schema:name nlm_unique_id
    170 schema:value 9604648
    171 rdf:type schema:PropertyValue
    172 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    173 schema:name Biological Sciences
    174 rdf:type schema:DefinedTerm
    175 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    176 schema:name Genetics
    177 rdf:type schema:DefinedTerm
    178 sg:journal.1115214 schema:issn 1087-0156
    179 1546-1696
    180 schema:name Nature Biotechnology
    181 rdf:type schema:Periodical
    182 sg:person.01014266246.80 schema:affiliation https://www.grid.ac/institutes/grid.38142.3c
    183 schema:familyName Huangfu
    184 schema:givenName Danwei
    185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01014266246.80
    186 rdf:type schema:Person
    187 sg:person.01054122426.39 schema:affiliation https://www.grid.ac/institutes/grid.5477.1
    188 schema:familyName Eijkelenboom
    189 schema:givenName Astrid
    190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01054122426.39
    191 rdf:type schema:Person
    192 sg:person.01143207360.34 schema:affiliation https://www.grid.ac/institutes/grid.419082.6
    193 schema:familyName Osafune
    194 schema:givenName Kenji
    195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01143207360.34
    196 rdf:type schema:Person
    197 sg:person.01164235020.37 schema:affiliation https://www.grid.ac/institutes/grid.38142.3c
    198 schema:familyName Melton
    199 schema:givenName Douglas A
    200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164235020.37
    201 rdf:type schema:Person
    202 sg:person.014605715475.58 schema:affiliation https://www.grid.ac/institutes/grid.116068.8
    203 schema:familyName Guo
    204 schema:givenName Wenjun
    205 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014605715475.58
    206 rdf:type schema:Person
    207 sg:person.016474257017.70 schema:affiliation https://www.grid.ac/institutes/grid.38142.3c
    208 schema:familyName Chen
    209 schema:givenName Shuibing
    210 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016474257017.70
    211 rdf:type schema:Person
    212 sg:person.0744246221.84 schema:affiliation https://www.grid.ac/institutes/grid.38142.3c
    213 schema:familyName Maehr
    214 schema:givenName René
    215 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744246221.84
    216 rdf:type schema:Person
    217 sg:pub.10.1038/182064a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036323125
    218 https://doi.org/10.1038/182064a0
    219 rdf:type schema:CreativeWork
    220 sg:pub.10.1038/28615 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005040238
    221 https://doi.org/10.1038/28615
    222 rdf:type schema:CreativeWork
    223 sg:pub.10.1038/385810a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043346431
    224 https://doi.org/10.1038/385810a0
    225 rdf:type schema:CreativeWork
    226 sg:pub.10.1038/448260a schema:sameAs https://app.dimensions.ai/details/publication/pub.1009764115
    227 https://doi.org/10.1038/448260a
    228 rdf:type schema:CreativeWork
    229 sg:pub.10.1038/nature05934 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002347899
    230 https://doi.org/10.1038/nature05934
    231 rdf:type schema:CreativeWork
    232 sg:pub.10.1038/nature05944 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019109862
    233 https://doi.org/10.1038/nature05944
    234 rdf:type schema:CreativeWork
    235 sg:pub.10.1038/nature06357 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046768545
    236 https://doi.org/10.1038/nature06357
    237 rdf:type schema:CreativeWork
    238 sg:pub.10.1038/nature06534 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011992920
    239 https://doi.org/10.1038/nature06534
    240 rdf:type schema:CreativeWork
    241 sg:pub.10.1038/nature07056 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037743725
    242 https://doi.org/10.1038/nature07056
    243 rdf:type schema:CreativeWork
    244 sg:pub.10.1038/nature07061 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017686107
    245 https://doi.org/10.1038/nature07061
    246 rdf:type schema:CreativeWork
    247 sg:pub.10.1038/nbt1106-1363 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016800008
    248 https://doi.org/10.1038/nbt1106-1363
    249 rdf:type schema:CreativeWork
    250 sg:pub.10.1038/nbt1163 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051219192
    251 https://doi.org/10.1038/nbt1163
    252 rdf:type schema:CreativeWork
    253 sg:pub.10.1038/nbt1310 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024578139
    254 https://doi.org/10.1038/nbt1310
    255 rdf:type schema:CreativeWork
    256 sg:pub.10.1038/nbt1335 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049953935
    257 https://doi.org/10.1038/nbt1335
    258 rdf:type schema:CreativeWork
    259 sg:pub.10.1038/nbt1374 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002744128
    260 https://doi.org/10.1038/nbt1374
    261 rdf:type schema:CreativeWork
    262 sg:pub.10.1038/nbt1383 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039633163
    263 https://doi.org/10.1038/nbt1383
    264 rdf:type schema:CreativeWork
    265 sg:pub.10.1038/nbt1418 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014318073
    266 https://doi.org/10.1038/nbt1418
    267 rdf:type schema:CreativeWork
    268 sg:pub.10.1038/nm837 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023534021
    269 https://doi.org/10.1038/nm837
    270 rdf:type schema:CreativeWork
    271 https://doi.org/10.1006/dbio.2000.9912 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053693663
    272 rdf:type schema:CreativeWork
    273 https://doi.org/10.1016/j.cell.2005.08.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049638413
    274 rdf:type schema:CreativeWork
    275 https://doi.org/10.1016/j.cell.2006.07.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014573758
    276 rdf:type schema:CreativeWork
    277 https://doi.org/10.1016/j.cell.2007.11.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010904856
    278 rdf:type schema:CreativeWork
    279 https://doi.org/10.1016/j.cell.2007.11.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000473144
    280 rdf:type schema:CreativeWork
    281 https://doi.org/10.1016/j.stem.2007.05.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029649252
    282 rdf:type schema:CreativeWork
    283 https://doi.org/10.1016/j.stem.2007.08.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011219533
    284 rdf:type schema:CreativeWork
    285 https://doi.org/10.1016/j.stem.2007.12.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012875023
    286 rdf:type schema:CreativeWork
    287 https://doi.org/10.1016/j.stem.2008.05.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006466689
    288 rdf:type schema:CreativeWork
    289 https://doi.org/10.1016/j.stem.2008.06.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013817335
    290 rdf:type schema:CreativeWork
    291 https://doi.org/10.1016/s0896-6273(00)00083-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005164056
    292 rdf:type schema:CreativeWork
    293 https://doi.org/10.1016/s0960-9822(01)00459-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037472156
    294 rdf:type schema:CreativeWork
    295 https://doi.org/10.1056/nejmsr040330 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022304644
    296 rdf:type schema:CreativeWork
    297 https://doi.org/10.1073/pnas.0711983105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024490582
    298 rdf:type schema:CreativeWork
    299 https://doi.org/10.1074/jbc.c400479200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007614091
    300 rdf:type schema:CreativeWork
    301 https://doi.org/10.1091/mbc.e07-01-0029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018776426
    302 rdf:type schema:CreativeWork
    303 https://doi.org/10.1126/science.1116447 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032194044
    304 rdf:type schema:CreativeWork
    305 https://doi.org/10.1126/science.1151526 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048905674
    306 rdf:type schema:CreativeWork
    307 https://doi.org/10.1126/science.282.5391.1145 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028898955
    308 rdf:type schema:CreativeWork
    309 https://doi.org/10.1146/annurev.med.58.071105.110848 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019541541
    310 rdf:type schema:CreativeWork
    311 https://www.grid.ac/institutes/grid.116068.8 schema:alternateName Massachusetts Institute of Technology
    312 schema:name The Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA.
    313 rdf:type schema:Organization
    314 https://www.grid.ac/institutes/grid.38142.3c schema:alternateName Harvard University
    315 schema:name Department of Stem Cell and Regenerative Biology, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts 02138, USA.
    316 rdf:type schema:Organization
    317 https://www.grid.ac/institutes/grid.419082.6 schema:alternateName Japan Science and Technology Agency
    318 schema:name Department of Stem Cell and Regenerative Biology, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts 02138, USA.
    319 ICORP Organ Regeneration Project, Japan Science and Technology Agency (JST), 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.
    320 rdf:type schema:Organization
    321 https://www.grid.ac/institutes/grid.5477.1 schema:alternateName Utrecht University
    322 schema:name Biomedical Sciences, Utrecht University, The Netherlands.
    323 Department of Stem Cell and Regenerative Biology, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts 02138, USA.
    324 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...