DMY is a Y-specific DM-domain gene required for male development in the medaka fish View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2002-05-12

AUTHORS

Masaru Matsuda, Yoshitaka Nagahama, Ai Shinomiya, Tadashi Sato, Chika Matsuda, Tohru Kobayashi, Craig E. Morrey, Naoki Shibata, Shuichi Asakawa, Nobuyoshi Shimizu, Hiroshi Hori, Satoshi Hamaguchi, Mitsuru Sakaizumi

ABSTRACT

Although the sex-determining gene Sry has been identified in mammals1, no comparable genes have been found in non-mammalian vertebrates. Here, we used recombinant breakpoint analysis to restrict the sex-determining region in medaka fish (Oryzias latipes) to a 530-kilobase (kb) stretch of the Y chromosome. Deletion analysis of the Y chromosome of a congenic XY female further shortened the region to 250 kb. Shotgun sequencing of this region predicted 27 genes. Three of these genes were expressed during sexual differentiation. However, only the DM-related2PG17 was Y specific; we thus named it DMY. Two naturally occurring mutations establish DMY's critical role in male development. The first heritable mutant—a single insertion in exon 3 and the subsequent truncation of DMY—resulted in all XY female offspring. Similarly, the second XY mutant female showed reduced DMY expression with a high proportion of XY female offspring. During normal development, DMY is expressed only in somatic cells of XY gonads. These findings strongly suggest that the sex-specific DMY is required for testicular development and is a prime candidate for the medaka sex-determining gene. More... »

PAGES

559-563

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nature751

DOI

http://dx.doi.org/10.1038/nature751

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1052603510

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12037570


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals, Congenic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromosome Breakage", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cloning, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Crosses, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fish Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation, Developmental", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genotype", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "In Situ Hybridization", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "In Situ Hybridization, Fluorescence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oryzias", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phenotype", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Physical Chromosome Mapping", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Structure, Tertiary", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Messenger", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Deletion", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sex Determination Processes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "X Chromosome", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Y Chromosome", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Laboratory of Reproductive Biology, National Institute for Basic Biology, 444-8585, Okazaki, Japan", 
          "id": "http://www.grid.ac/institutes/grid.419396.0", 
          "name": [
            "Laboratory of Reproductive Biology, National Institute for Basic Biology, 444-8585, Okazaki, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Matsuda", 
        "givenName": "Masaru", 
        "id": "sg:person.01235513654.16", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235513654.16"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratory of Reproductive Biology, National Institute for Basic Biology, 444-8585, Okazaki, Japan", 
          "id": "http://www.grid.ac/institutes/grid.419396.0", 
          "name": [
            "Laboratory of Reproductive Biology, National Institute for Basic Biology, 444-8585, Okazaki, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nagahama", 
        "givenName": "Yoshitaka", 
        "id": "sg:person.01203110226.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01203110226.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Graduate School of Science and Technology, Niigata University, 950-2181, Niigata; Ikarashi, Japan", 
          "id": "http://www.grid.ac/institutes/grid.260975.f", 
          "name": [
            "Graduate School of Science and Technology, Niigata University, 950-2181, Niigata; Ikarashi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shinomiya", 
        "givenName": "Ai", 
        "id": "sg:person.01203456501.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01203456501.08"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Graduate School of Science and Technology, Niigata University, 950-2181, Niigata; Ikarashi, Japan", 
          "id": "http://www.grid.ac/institutes/grid.260975.f", 
          "name": [
            "Graduate School of Science and Technology, Niigata University, 950-2181, Niigata; Ikarashi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sato", 
        "givenName": "Tadashi", 
        "id": "sg:person.01153415701.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153415701.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratory of Reproductive Biology, National Institute for Basic Biology, 444-8585, Okazaki, Japan", 
          "id": "http://www.grid.ac/institutes/grid.419396.0", 
          "name": [
            "Laboratory of Reproductive Biology, National Institute for Basic Biology, 444-8585, Okazaki, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Matsuda", 
        "givenName": "Chika", 
        "id": "sg:person.0767121202.12", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0767121202.12"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratory of Reproductive Biology, National Institute for Basic Biology, 444-8585, Okazaki, Japan", 
          "id": "http://www.grid.ac/institutes/grid.419396.0", 
          "name": [
            "Laboratory of Reproductive Biology, National Institute for Basic Biology, 444-8585, Okazaki, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kobayashi", 
        "givenName": "Tohru", 
        "id": "sg:person.01302465205.50", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01302465205.50"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratory of Reproductive Biology, National Institute for Basic Biology, 444-8585, Okazaki, Japan", 
          "id": "http://www.grid.ac/institutes/grid.419396.0", 
          "name": [
            "Laboratory of Reproductive Biology, National Institute for Basic Biology, 444-8585, Okazaki, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Morrey", 
        "givenName": "Craig E.", 
        "id": "sg:person.0770476172.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770476172.51"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biology, Faculty of Science, Shinshu University, Asahi 3-1-1, Matsumoto, 390-8621, Nagano, Japan", 
          "id": "http://www.grid.ac/institutes/grid.263518.b", 
          "name": [
            "Department of Biology, Faculty of Science, Shinshu University, Asahi 3-1-1, Matsumoto, 390-8621, Nagano, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shibata", 
        "givenName": "Naoki", 
        "id": "sg:person.01240353317.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01240353317.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, 160-8582, TokyoShinjuku-ku, Japan", 
          "id": "http://www.grid.ac/institutes/grid.26091.3c", 
          "name": [
            "Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, 160-8582, TokyoShinjuku-ku, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Asakawa", 
        "givenName": "Shuichi", 
        "id": "sg:person.01103425316.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01103425316.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, 160-8582, TokyoShinjuku-ku, Japan", 
          "id": "http://www.grid.ac/institutes/grid.26091.3c", 
          "name": [
            "Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, 160-8582, TokyoShinjuku-ku, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shimizu", 
        "givenName": "Nobuyoshi", 
        "id": "sg:person.010061633617.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010061633617.31"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Graduate School of Science, Nagoya University, 464-8602, NagoyaChikusa-ku, Japan", 
          "id": "http://www.grid.ac/institutes/grid.27476.30", 
          "name": [
            "Graduate School of Science, Nagoya University, 464-8602, NagoyaChikusa-ku, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hori", 
        "givenName": "Hiroshi", 
        "id": "sg:person.01360712444.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01360712444.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Graduate School of Science and Technology, Niigata University, 950-2181, Niigata; Ikarashi, Japan", 
          "id": "http://www.grid.ac/institutes/grid.260975.f", 
          "name": [
            "Graduate School of Science and Technology, Niigata University, 950-2181, Niigata; Ikarashi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hamaguchi", 
        "givenName": "Satoshi", 
        "id": "sg:person.01274600771.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01274600771.22"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Graduate School of Science and Technology, Niigata University, 950-2181, Niigata; Ikarashi, Japan", 
          "id": "http://www.grid.ac/institutes/grid.260975.f", 
          "name": [
            "Graduate School of Science and Technology, Niigata University, 950-2181, Niigata; Ikarashi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sakaizumi", 
        "givenName": "Mitsuru", 
        "id": "sg:person.01342714171.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342714171.44"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf00206337", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010964815", 
          "https://doi.org/10.1007/bf00206337"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1013755701696", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031415992", 
          "https://doi.org/10.1023/a:1013755701696"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/346240a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041191003", 
          "https://doi.org/10.1038/346240a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35618", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031990764", 
          "https://doi.org/10.1038/35618"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/45130", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034331959", 
          "https://doi.org/10.1038/45130"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2002-05-12", 
    "datePublishedReg": "2002-05-12", 
    "description": "Although the sex-determining gene Sry has been identified in mammals1, no comparable genes have been found in non-mammalian vertebrates. Here, we used recombinant breakpoint analysis to restrict the sex-determining region in medaka fish (Oryzias latipes) to a 530-kilobase (kb) stretch of the Y chromosome. Deletion analysis of the Y chromosome of a congenic XY female further shortened the region to 250\u2009kb. Shotgun sequencing of this region predicted 27 genes. Three of these genes were expressed during sexual differentiation. However, only the DM-related2PG17 was Y specific; we thus named it DMY. Two naturally occurring mutations establish DMY's critical role in male development. The first heritable mutant\u2014a single insertion in exon 3 and the subsequent truncation of DMY\u2014resulted in all XY female offspring. Similarly, the second XY mutant female showed reduced DMY expression with a high proportion of XY female offspring. During normal development, DMY is expressed only in somatic cells of XY gonads. These findings strongly suggest that the sex-specific DMY is required for testicular development and is a prime candidate for the medaka sex-determining gene.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/nature751", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0028-0836", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6888", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "417"
      }
    ], 
    "keywords": [
      "medaka fish", 
      "male development", 
      "sex-determining gene SRY", 
      "Y chromosome", 
      "sex-determining genes", 
      "DM domain genes", 
      "non-mammalian vertebrates", 
      "sex-determining region", 
      "heritable mutants", 
      "gene SRY", 
      "critical role", 
      "XY gonads", 
      "DMY expression", 
      "mutant females", 
      "comparable genes", 
      "deletion analysis", 
      "somatic cells", 
      "shotgun sequencing", 
      "XY females", 
      "genes", 
      "female offspring", 
      "normal development", 
      "sexual differentiation", 
      "exon 3", 
      "chromosomes", 
      "single insertion", 
      "DMY", 
      "testicular development", 
      "breakpoint analysis", 
      "fish", 
      "subsequent truncation", 
      "offspring", 
      "vertebrates", 
      "SRY", 
      "mutants", 
      "mammals1", 
      "prime candidate", 
      "kb", 
      "sequencing", 
      "gonads", 
      "mutations", 
      "differentiation", 
      "region", 
      "expression", 
      "higher proportion", 
      "females", 
      "cells", 
      "stretch", 
      "development", 
      "truncation", 
      "role", 
      "insertion", 
      "analysis", 
      "candidates", 
      "proportion", 
      "findings"
    ], 
    "name": "DMY is a Y-specific DM-domain gene required for male development in the medaka fish", 
    "pagination": "559-563", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1052603510"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nature751"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "12037570"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nature751", 
      "https://app.dimensions.ai/details/publication/pub.1052603510"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-08-04T16:53", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_358.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/nature751"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature751'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature751'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature751'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature751'


 

This table displays all metadata directly associated to this object as RDF triples.

329 TRIPLES      21 PREDICATES      110 URIs      97 LITERALS      31 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nature751 schema:about N20e780fd28c64c3193b6e03c2a362f18
2 N23202e5138234ea3b11b125d91e28f16
3 N31b308ad9c114e0b8678173f747e15b4
4 N3eec2a659a5f4271b88dae14f7e37848
5 N416466d53d464a129d066508ebfc3c09
6 N4a5e12feec1d48959a13b57c5532e58f
7 N558e7eeae89e426d821a3032173e9e3e
8 N658e3eed90a44cf98e548fddc39a3ba6
9 N6bc7e4e4fa174091a7add7c7df298335
10 N7a905a6bd8124cbda80e7ae17f898f79
11 N8cd4a87ee7064c3b86bf9a31967040cc
12 N91ed3da3b6644140a8cf8bb3fa52cd0e
13 N94ea9d1f2eb442688becd8aee3a9bb46
14 N97e2b241b58d4648b22917a7363a0f90
15 Na1f75511f0ac4f77b6ba6ed61c6b3143
16 Nc5ea0e08ce95403d8d3e7f7323bda6c8
17 Nd8055dfc011e4287872641b9f5ad9b28
18 Ndd1cb45b3d844c87a711af81dbbdd3e0
19 Ndefec7cad8a048ad8241dac19f75e45b
20 Ne33740c4f9df4ae096a8146cbde06807
21 Ne7cf46305b294f8795d851c1a8f8bbef
22 Nf355e01f5cee4419900d3344497c2653
23 Nfcc187d0cf724c0eb14661edfd569e86
24 Nfe8c1053e39e44558bac71de2c1bad9f
25 anzsrc-for:06
26 anzsrc-for:0604
27 schema:author N4fc9f6ae28d14fa9b2d2698b965851b4
28 schema:citation sg:pub.10.1007/bf00206337
29 sg:pub.10.1023/a:1013755701696
30 sg:pub.10.1038/346240a0
31 sg:pub.10.1038/35618
32 sg:pub.10.1038/45130
33 schema:datePublished 2002-05-12
34 schema:datePublishedReg 2002-05-12
35 schema:description Although the sex-determining gene Sry has been identified in mammals1, no comparable genes have been found in non-mammalian vertebrates. Here, we used recombinant breakpoint analysis to restrict the sex-determining region in medaka fish (Oryzias latipes) to a 530-kilobase (kb) stretch of the Y chromosome. Deletion analysis of the Y chromosome of a congenic XY female further shortened the region to 250 kb. Shotgun sequencing of this region predicted 27 genes. Three of these genes were expressed during sexual differentiation. However, only the DM-related2PG17 was Y specific; we thus named it DMY. Two naturally occurring mutations establish DMY's critical role in male development. The first heritable mutant—a single insertion in exon 3 and the subsequent truncation of DMY—resulted in all XY female offspring. Similarly, the second XY mutant female showed reduced DMY expression with a high proportion of XY female offspring. During normal development, DMY is expressed only in somatic cells of XY gonads. These findings strongly suggest that the sex-specific DMY is required for testicular development and is a prime candidate for the medaka sex-determining gene.
36 schema:genre article
37 schema:isAccessibleForFree false
38 schema:isPartOf N1baee746b9264486bb596e93716266f8
39 Nff55fb07df80419285730f5940a75924
40 sg:journal.1018957
41 schema:keywords DM domain genes
42 DMY
43 DMY expression
44 SRY
45 XY females
46 XY gonads
47 Y chromosome
48 analysis
49 breakpoint analysis
50 candidates
51 cells
52 chromosomes
53 comparable genes
54 critical role
55 deletion analysis
56 development
57 differentiation
58 exon 3
59 expression
60 female offspring
61 females
62 findings
63 fish
64 gene SRY
65 genes
66 gonads
67 heritable mutants
68 higher proportion
69 insertion
70 kb
71 male development
72 mammals1
73 medaka fish
74 mutant females
75 mutants
76 mutations
77 non-mammalian vertebrates
78 normal development
79 offspring
80 prime candidate
81 proportion
82 region
83 role
84 sequencing
85 sex-determining gene SRY
86 sex-determining genes
87 sex-determining region
88 sexual differentiation
89 shotgun sequencing
90 single insertion
91 somatic cells
92 stretch
93 subsequent truncation
94 testicular development
95 truncation
96 vertebrates
97 schema:name DMY is a Y-specific DM-domain gene required for male development in the medaka fish
98 schema:pagination 559-563
99 schema:productId N4f1aff573ad94803802057d76527e951
100 N7f8fc882cac644d3815290a610ec6afa
101 N855663dda06d49de8e8dadba69e69861
102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052603510
103 https://doi.org/10.1038/nature751
104 schema:sdDatePublished 2022-08-04T16:53
105 schema:sdLicense https://scigraph.springernature.com/explorer/license/
106 schema:sdPublisher Nb9bc4cbd947d481e89d44ce720f1e229
107 schema:url https://doi.org/10.1038/nature751
108 sgo:license sg:explorer/license/
109 sgo:sdDataset articles
110 rdf:type schema:ScholarlyArticle
111 N19a412e2fc524dae9cd554da4c7d546e rdf:first sg:person.01342714171.44
112 rdf:rest rdf:nil
113 N1baee746b9264486bb596e93716266f8 schema:issueNumber 6888
114 rdf:type schema:PublicationIssue
115 N202a5873d1004ddb8011f5bdd8c2e9a1 rdf:first sg:person.0770476172.51
116 rdf:rest N529ed65d8110449aad8ca5327a17ed7f
117 N209318b038144e328d7688e135738cf9 rdf:first sg:person.01103425316.62
118 rdf:rest Na58dac058a4649e7aca043ab30b6cb42
119 N20e780fd28c64c3193b6e03c2a362f18 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Cloning, Molecular
121 rdf:type schema:DefinedTerm
122 N23202e5138234ea3b11b125d91e28f16 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Phenotype
124 rdf:type schema:DefinedTerm
125 N31b308ad9c114e0b8678173f747e15b4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Chromosome Breakage
127 rdf:type schema:DefinedTerm
128 N3a38ddb847944213bf7f7fb513fc4381 rdf:first sg:person.01360712444.07
129 rdf:rest Nb41ab110cbb943c081fcbc334e6d5934
130 N3eec2a659a5f4271b88dae14f7e37848 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Gene Expression Regulation, Developmental
132 rdf:type schema:DefinedTerm
133 N416466d53d464a129d066508ebfc3c09 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Sex Determination Processes
135 rdf:type schema:DefinedTerm
136 N476cd24614f04ec99b193609d637a390 rdf:first sg:person.01203110226.44
137 rdf:rest Nf13a1a13dfcc4930996446a8918801e2
138 N4a5e12feec1d48959a13b57c5532e58f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Amino Acid Sequence
140 rdf:type schema:DefinedTerm
141 N4f1aff573ad94803802057d76527e951 schema:name dimensions_id
142 schema:value pub.1052603510
143 rdf:type schema:PropertyValue
144 N4fc9f6ae28d14fa9b2d2698b965851b4 rdf:first sg:person.01235513654.16
145 rdf:rest N476cd24614f04ec99b193609d637a390
146 N5046ad36bc794ff89139b531e36d001a rdf:first sg:person.01153415701.00
147 rdf:rest Nf183574e3d0b4374b416b2616dba792b
148 N529ed65d8110449aad8ca5327a17ed7f rdf:first sg:person.01240353317.07
149 rdf:rest N209318b038144e328d7688e135738cf9
150 N558e7eeae89e426d821a3032173e9e3e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name RNA, Messenger
152 rdf:type schema:DefinedTerm
153 N658e3eed90a44cf98e548fddc39a3ba6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Animals
155 rdf:type schema:DefinedTerm
156 N6bc7e4e4fa174091a7add7c7df298335 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Molecular Sequence Data
158 rdf:type schema:DefinedTerm
159 N7a905a6bd8124cbda80e7ae17f898f79 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Mutation
161 rdf:type schema:DefinedTerm
162 N7f8fc882cac644d3815290a610ec6afa schema:name pubmed_id
163 schema:value 12037570
164 rdf:type schema:PropertyValue
165 N855663dda06d49de8e8dadba69e69861 schema:name doi
166 schema:value 10.1038/nature751
167 rdf:type schema:PropertyValue
168 N8cd4a87ee7064c3b86bf9a31967040cc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
169 schema:name Crosses, Genetic
170 rdf:type schema:DefinedTerm
171 N91ed3da3b6644140a8cf8bb3fa52cd0e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
172 schema:name Genotype
173 rdf:type schema:DefinedTerm
174 N94ea9d1f2eb442688becd8aee3a9bb46 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
175 schema:name Female
176 rdf:type schema:DefinedTerm
177 N97e2b241b58d4648b22917a7363a0f90 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
178 schema:name Physical Chromosome Mapping
179 rdf:type schema:DefinedTerm
180 N986ea8bdd54945c3b719a819390b7897 rdf:first sg:person.01302465205.50
181 rdf:rest N202a5873d1004ddb8011f5bdd8c2e9a1
182 Na1f75511f0ac4f77b6ba6ed61c6b3143 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
183 schema:name Sequence Deletion
184 rdf:type schema:DefinedTerm
185 Na58dac058a4649e7aca043ab30b6cb42 rdf:first sg:person.010061633617.31
186 rdf:rest N3a38ddb847944213bf7f7fb513fc4381
187 Nb41ab110cbb943c081fcbc334e6d5934 rdf:first sg:person.01274600771.22
188 rdf:rest N19a412e2fc524dae9cd554da4c7d546e
189 Nb9bc4cbd947d481e89d44ce720f1e229 schema:name Springer Nature - SN SciGraph project
190 rdf:type schema:Organization
191 Nc5ea0e08ce95403d8d3e7f7323bda6c8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
192 schema:name Oryzias
193 rdf:type schema:DefinedTerm
194 Nd8055dfc011e4287872641b9f5ad9b28 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
195 schema:name Animals, Congenic
196 rdf:type schema:DefinedTerm
197 Ndd1cb45b3d844c87a711af81dbbdd3e0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
198 schema:name Protein Structure, Tertiary
199 rdf:type schema:DefinedTerm
200 Ndefec7cad8a048ad8241dac19f75e45b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
201 schema:name In Situ Hybridization, Fluorescence
202 rdf:type schema:DefinedTerm
203 Ne33740c4f9df4ae096a8146cbde06807 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
204 schema:name Fish Proteins
205 rdf:type schema:DefinedTerm
206 Ne7cf46305b294f8795d851c1a8f8bbef schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
207 schema:name X Chromosome
208 rdf:type schema:DefinedTerm
209 Nf13a1a13dfcc4930996446a8918801e2 rdf:first sg:person.01203456501.08
210 rdf:rest N5046ad36bc794ff89139b531e36d001a
211 Nf183574e3d0b4374b416b2616dba792b rdf:first sg:person.0767121202.12
212 rdf:rest N986ea8bdd54945c3b719a819390b7897
213 Nf355e01f5cee4419900d3344497c2653 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
214 schema:name In Situ Hybridization
215 rdf:type schema:DefinedTerm
216 Nfcc187d0cf724c0eb14661edfd569e86 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
217 schema:name Y Chromosome
218 rdf:type schema:DefinedTerm
219 Nfe8c1053e39e44558bac71de2c1bad9f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
220 schema:name Male
221 rdf:type schema:DefinedTerm
222 Nff55fb07df80419285730f5940a75924 schema:volumeNumber 417
223 rdf:type schema:PublicationVolume
224 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
225 schema:name Biological Sciences
226 rdf:type schema:DefinedTerm
227 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
228 schema:name Genetics
229 rdf:type schema:DefinedTerm
230 sg:journal.1018957 schema:issn 0028-0836
231 1476-4687
232 schema:name Nature
233 schema:publisher Springer Nature
234 rdf:type schema:Periodical
235 sg:person.010061633617.31 schema:affiliation grid-institutes:grid.26091.3c
236 schema:familyName Shimizu
237 schema:givenName Nobuyoshi
238 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010061633617.31
239 rdf:type schema:Person
240 sg:person.01103425316.62 schema:affiliation grid-institutes:grid.26091.3c
241 schema:familyName Asakawa
242 schema:givenName Shuichi
243 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01103425316.62
244 rdf:type schema:Person
245 sg:person.01153415701.00 schema:affiliation grid-institutes:grid.260975.f
246 schema:familyName Sato
247 schema:givenName Tadashi
248 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153415701.00
249 rdf:type schema:Person
250 sg:person.01203110226.44 schema:affiliation grid-institutes:grid.419396.0
251 schema:familyName Nagahama
252 schema:givenName Yoshitaka
253 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01203110226.44
254 rdf:type schema:Person
255 sg:person.01203456501.08 schema:affiliation grid-institutes:grid.260975.f
256 schema:familyName Shinomiya
257 schema:givenName Ai
258 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01203456501.08
259 rdf:type schema:Person
260 sg:person.01235513654.16 schema:affiliation grid-institutes:grid.419396.0
261 schema:familyName Matsuda
262 schema:givenName Masaru
263 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235513654.16
264 rdf:type schema:Person
265 sg:person.01240353317.07 schema:affiliation grid-institutes:grid.263518.b
266 schema:familyName Shibata
267 schema:givenName Naoki
268 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01240353317.07
269 rdf:type schema:Person
270 sg:person.01274600771.22 schema:affiliation grid-institutes:grid.260975.f
271 schema:familyName Hamaguchi
272 schema:givenName Satoshi
273 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01274600771.22
274 rdf:type schema:Person
275 sg:person.01302465205.50 schema:affiliation grid-institutes:grid.419396.0
276 schema:familyName Kobayashi
277 schema:givenName Tohru
278 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01302465205.50
279 rdf:type schema:Person
280 sg:person.01342714171.44 schema:affiliation grid-institutes:grid.260975.f
281 schema:familyName Sakaizumi
282 schema:givenName Mitsuru
283 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342714171.44
284 rdf:type schema:Person
285 sg:person.01360712444.07 schema:affiliation grid-institutes:grid.27476.30
286 schema:familyName Hori
287 schema:givenName Hiroshi
288 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01360712444.07
289 rdf:type schema:Person
290 sg:person.0767121202.12 schema:affiliation grid-institutes:grid.419396.0
291 schema:familyName Matsuda
292 schema:givenName Chika
293 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0767121202.12
294 rdf:type schema:Person
295 sg:person.0770476172.51 schema:affiliation grid-institutes:grid.419396.0
296 schema:familyName Morrey
297 schema:givenName Craig E.
298 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0770476172.51
299 rdf:type schema:Person
300 sg:pub.10.1007/bf00206337 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010964815
301 https://doi.org/10.1007/bf00206337
302 rdf:type schema:CreativeWork
303 sg:pub.10.1023/a:1013755701696 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031415992
304 https://doi.org/10.1023/a:1013755701696
305 rdf:type schema:CreativeWork
306 sg:pub.10.1038/346240a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041191003
307 https://doi.org/10.1038/346240a0
308 rdf:type schema:CreativeWork
309 sg:pub.10.1038/35618 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031990764
310 https://doi.org/10.1038/35618
311 rdf:type schema:CreativeWork
312 sg:pub.10.1038/45130 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034331959
313 https://doi.org/10.1038/45130
314 rdf:type schema:CreativeWork
315 grid-institutes:grid.26091.3c schema:alternateName Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, 160-8582, TokyoShinjuku-ku, Japan
316 schema:name Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, 160-8582, TokyoShinjuku-ku, Japan
317 rdf:type schema:Organization
318 grid-institutes:grid.260975.f schema:alternateName Graduate School of Science and Technology, Niigata University, 950-2181, Niigata; Ikarashi, Japan
319 schema:name Graduate School of Science and Technology, Niigata University, 950-2181, Niigata; Ikarashi, Japan
320 rdf:type schema:Organization
321 grid-institutes:grid.263518.b schema:alternateName Department of Biology, Faculty of Science, Shinshu University, Asahi 3-1-1, Matsumoto, 390-8621, Nagano, Japan
322 schema:name Department of Biology, Faculty of Science, Shinshu University, Asahi 3-1-1, Matsumoto, 390-8621, Nagano, Japan
323 rdf:type schema:Organization
324 grid-institutes:grid.27476.30 schema:alternateName Graduate School of Science, Nagoya University, 464-8602, NagoyaChikusa-ku, Japan
325 schema:name Graduate School of Science, Nagoya University, 464-8602, NagoyaChikusa-ku, Japan
326 rdf:type schema:Organization
327 grid-institutes:grid.419396.0 schema:alternateName Laboratory of Reproductive Biology, National Institute for Basic Biology, 444-8585, Okazaki, Japan
328 schema:name Laboratory of Reproductive Biology, National Institute for Basic Biology, 444-8585, Okazaki, Japan
329 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...