DMY is a Y-specific DM-domain gene required for male development in the medaka fish View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2002-05-12

AUTHORS

Masaru Matsuda, Yoshitaka Nagahama, Ai Shinomiya, Tadashi Sato, Chika Matsuda, Tohru Kobayashi, Craig E. Morrey, Naoki Shibata, Shuichi Asakawa, Nobuyoshi Shimizu, Hiroshi Hori, Satoshi Hamaguchi, Mitsuru Sakaizumi

ABSTRACT

Although the sex-determining gene Sry has been identified in mammals1, no comparable genes have been found in non-mammalian vertebrates. Here, we used recombinant breakpoint analysis to restrict the sex-determining region in medaka fish (Oryzias latipes) to a 530-kilobase (kb) stretch of the Y chromosome. Deletion analysis of the Y chromosome of a congenic XY female further shortened the region to 250 kb. Shotgun sequencing of this region predicted 27 genes. Three of these genes were expressed during sexual differentiation. However, only the DM-related2PG17 was Y specific; we thus named it DMY. Two naturally occurring mutations establish DMY's critical role in male development. The first heritable mutant—a single insertion in exon 3 and the subsequent truncation of DMY—resulted in all XY female offspring. Similarly, the second XY mutant female showed reduced DMY expression with a high proportion of XY female offspring. During normal development, DMY is expressed only in somatic cells of XY gonads. These findings strongly suggest that the sex-specific DMY is required for testicular development and is a prime candidate for the medaka sex-determining gene. More... »

PAGES

559-563

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nature751

DOI

http://dx.doi.org/10.1038/nature751

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1052603510

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12037570


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35 schema:description Although the sex-determining gene Sry has been identified in mammals1, no comparable genes have been found in non-mammalian vertebrates. Here, we used recombinant breakpoint analysis to restrict the sex-determining region in medaka fish (Oryzias latipes) to a 530-kilobase (kb) stretch of the Y chromosome. Deletion analysis of the Y chromosome of a congenic XY female further shortened the region to 250 kb. Shotgun sequencing of this region predicted 27 genes. Three of these genes were expressed during sexual differentiation. However, only the DM-related2PG17 was Y specific; we thus named it DMY. Two naturally occurring mutations establish DMY's critical role in male development. The first heritable mutant—a single insertion in exon 3 and the subsequent truncation of DMY—resulted in all XY female offspring. Similarly, the second XY mutant female showed reduced DMY expression with a high proportion of XY female offspring. During normal development, DMY is expressed only in somatic cells of XY gonads. These findings strongly suggest that the sex-specific DMY is required for testicular development and is a prime candidate for the medaka sex-determining gene.
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42 DMY
43 DMY expression
44 SRY
45 XY females
46 XY gonads
47 Y chromosome
48 analysis
49 breakpoint analysis
50 candidates
51 cells
52 chromosomes
53 comparable genes
54 critical role
55 deletion analysis
56 development
57 differentiation
58 exon 3
59 expression
60 female offspring
61 females
62 findings
63 fish
64 gene SRY
65 genes
66 gonads
67 heritable mutants
68 higher proportion
69 insertion
70 kb
71 male development
72 mammals1
73 medaka fish
74 mutant females
75 mutants
76 mutations
77 non-mammalian vertebrates
78 normal development
79 offspring
80 prime candidate
81 proportion
82 region
83 role
84 sequencing
85 sex-determining gene SRY
86 sex-determining genes
87 sex-determining region
88 sexual differentiation
89 shotgun sequencing
90 single insertion
91 somatic cells
92 stretch
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94 testicular development
95 truncation
96 vertebrates
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