Asymmetric division of contractile domains couples cell positioning and fate specification View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-08

AUTHORS

Jean-Léon Maître, Hervé Turlier, Rukshala Illukkumbura, Björn Eismann, Ritsuya Niwayama, François Nédélec, Takashi Hiiragi

ABSTRACT

During pre-implantation development, the mammalian embryo self-organizes into the blastocyst, which consists of an epithelial layer encapsulating the inner-cell mass (ICM) giving rise to all embryonic tissues. In mice, oriented cell division, apicobasal polarity and actomyosin contractility are thought to contribute to the formation of the ICM. However, how these processes work together remains unclear. Here we show that asymmetric segregation of the apical domain generates blastomeres with different contractilities, which triggers their sorting into inner and outer positions. Three-dimensional physical modelling of embryo morphogenesis reveals that cells internalize only when differences in surface contractility exceed a predictable threshold. We validate this prediction using biophysical measurements, and successfully redirect cell sorting within the developing blastocyst using maternal myosin (Myh9)-knockout chimaeric embryos. Finally, we find that loss of contractility causes blastomeres to show ICM-like markers, regardless of their position. In particular, contractility controls Yap subcellular localization, raising the possibility that mechanosensing occurs during blastocyst lineage specification. We conclude that contractility couples the positioning and fate specification of blastomeres. We propose that this ensures the robust self-organization of blastomeres into the blastocyst, which confers remarkable regulative capacities to mammalian embryos. More... »

PAGES

344

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nature18958

DOI

http://dx.doi.org/10.1038/nature18958

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1005109746

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27487217


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