Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 Å resolution View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2016-02-01

AUTHORS

Thi Hoang Duong Nguyen, Wojciech P. Galej, Xiao-chen Bai, Chris Oubridge, Andrew J. Newman, Sjors H. W. Scheres, Kiyoshi Nagai

ABSTRACT

U4/U6.U5 tri-snRNP represents a substantial part of the spliceosome before activation. A cryo-electron microscopy structure of Saccharomyces cerevisiae U4/U6.U5 tri-snRNP at 3.7 Å resolution led to an essentially complete atomic model comprising 30 proteins plus U4/U6 and U5 small nuclear RNAs (snRNAs). The structure reveals striking interweaving interactions of the protein and RNA components, including extended polypeptides penetrating into subunit interfaces. The invariant ACAGAGA sequence of U6 snRNA, which base-pairs with the 5'-splice site during catalytic activation, forms a hairpin stabilized by Dib1 and Prp8 while the adjacent nucleotides interact with the exon binding loop 1 of U5 snRNA. Snu114 harbours GTP, but its putative catalytic histidine is held away from the γ-phosphate by hydrogen bonding to a tyrosine in the amino-terminal domain of Prp8. Mutation of this histidine to alanine has no detectable effect on yeast growth. The structure provides important new insights into the spliceosome activation process leading to the formation of the catalytic centre. More... »

PAGES

298

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nature16940

DOI

http://dx.doi.org/10.1038/nature16940

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026484180

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26829225


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Pairing", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Catalytic Domain", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cryoelectron Microscopy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Helicases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Exons", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Guanosine Triphosphate", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrogen Bonding", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nucleic Acid Conformation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA Splice Sites", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Small Nuclear", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ribonucleoprotein, U4-U6 Small Nuclear", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ribonucleoprotein, U5 Small Nuclear", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ribonucleoproteins, Small Nuclear", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spliceosomes", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "MRC Laboratory of Molecular Biology", 
          "id": "https://www.grid.ac/institutes/grid.42475.30", 
          "name": [
            "MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nguyen", 
        "givenName": "Thi Hoang Duong", 
        "id": "sg:person.01123147727.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123147727.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "MRC Laboratory of Molecular Biology", 
          "id": "https://www.grid.ac/institutes/grid.42475.30", 
          "name": [
            "MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Galej", 
        "givenName": "Wojciech P.", 
        "id": "sg:person.01037540344.37", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037540344.37"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "MRC Laboratory of Molecular Biology", 
          "id": "https://www.grid.ac/institutes/grid.42475.30", 
          "name": [
            "MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bai", 
        "givenName": "Xiao-chen", 
        "id": "sg:person.013775516617.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013775516617.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "MRC Laboratory of Molecular Biology", 
          "id": "https://www.grid.ac/institutes/grid.42475.30", 
          "name": [
            "MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Oubridge", 
        "givenName": "Chris", 
        "id": "sg:person.01350114410.45", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01350114410.45"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "MRC Laboratory of Molecular Biology", 
          "id": "https://www.grid.ac/institutes/grid.42475.30", 
          "name": [
            "MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Newman", 
        "givenName": "Andrew J.", 
        "id": "sg:person.016205631512.60", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016205631512.60"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "MRC Laboratory of Molecular Biology", 
          "id": "https://www.grid.ac/institutes/grid.42475.30", 
          "name": [
            "MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Scheres", 
        "givenName": "Sjors H. W.", 
        "id": "sg:person.015537361467.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015537361467.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "MRC Laboratory of Molecular Biology", 
          "id": "https://www.grid.ac/institutes/grid.42475.30", 
          "name": [
            "MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nagai", 
        "givenName": "Kiyoshi", 
        "id": "sg:person.011244132717.80", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011244132717.80"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1107/s0907444910007493", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000235100"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444910007493", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000235100"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ultramic.2013.06.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000304364"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jsb.2006.06.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002622528"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/21.7.1555", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003890063"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsmb761", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004637296", 
          "https://doi.org/10.1038/nsmb761"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsmb761", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004637296", 
          "https://doi.org/10.1038/nsmb761"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m505060200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005737822"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m505060200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005737822"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2014.11.049", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008221827"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1412676111", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009047707"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.2727", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009301311", 
          "https://doi.org/10.1038/nmeth.2727"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.aac8159", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010514152"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1261/rna.040980.113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010894553"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsb923", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011470000", 
          "https://doi.org/10.1038/nsb923"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsb923", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011470000", 
          "https://doi.org/10.1038/nsb923"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.7554/elife.07320", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011907345"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsmb.2815", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012432420", 
          "https://doi.org/10.1038/nsmb.2815"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature12734", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013048897", 
          "https://doi.org/10.1038/nature12734"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1235490", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015444206"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/16.13.4092", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016622427"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2006.05.043", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019947136"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmbi.1998.2183", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019972929"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1097-2765(00)80174-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021674452"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.199307.112", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022811615"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.7554/elife.11182", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023050774"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(92)90149-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023769788"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2009.09.040", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023972722"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.7554/elife.03665", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024693389"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.95.8.4188", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025132576"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0611591104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025657900"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.biophys.35.040405.101953", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026456216"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/emboj.2009.171", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026584924"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/emboj.2009.171", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026584924"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1534/genetics.105.042044", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026799986"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1534/genetics.105.042044", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026799986"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1534/genetics.105.042044", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026799986"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.82.2.488", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026991187"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s1399004714021683", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028026527"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s1399004714016241", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030014777"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444911001314", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032555428"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m303043200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032916959"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1261/rna.039024.113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033721337"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsmb.2951", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033777572", 
          "https://doi.org/10.1038/nsmb.2951"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev-biochem-062708-134043", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034927678"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.str.2013.04.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035257810"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/cshperspect.a003707", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036507675"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.ge.28.120194.000245", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037969108"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature14548", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040399474", 
          "https://doi.org/10.1038/nature14548"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.2115", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041024070", 
          "https://doi.org/10.1038/nmeth.2115"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature11843", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041916267", 
          "https://doi.org/10.1038/nature11843"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmbi.1999.3258", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042635354"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.aac7629", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043702597"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1261/rna.2319806", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045305977"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/cdf544", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045460564"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1047-8477(03)00069-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046234181"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1047-8477(03)00069-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046234181"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nprot.2008.62", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046618492", 
          "https://doi.org/10.1038/nprot.2008.62"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1261/rna.2229706", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048835685"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(07)00345-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048841316"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmbi.2000.3685", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048901077"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/18.16.4535", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049683698"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.2472", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050203089", 
          "https://doi.org/10.1038/nmeth.2472"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/30.4.1029", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050589838"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1153803", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050940808"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1534/genetics.109.107243", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052339206"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1534/genetics.109.107243", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052339206"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmb.2011.11.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052958331"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tibs.2014.10.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053316395"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1137924", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062455159"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.8266094", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062653902"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074678886", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074795547", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074957697", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082961828", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2016-02-01", 
    "datePublishedReg": "2016-02-01", 
    "description": "U4/U6.U5 tri-snRNP represents a substantial part of the spliceosome before activation. A cryo-electron microscopy structure of Saccharomyces cerevisiae U4/U6.U5 tri-snRNP at 3.7\u2009\u00c5 resolution led to an essentially complete atomic model comprising 30 proteins plus U4/U6 and U5 small nuclear RNAs (snRNAs). The structure reveals striking interweaving interactions of the protein and RNA components, including extended polypeptides penetrating into subunit interfaces. The invariant ACAGAGA sequence of U6 snRNA, which base-pairs with the 5'-splice site during catalytic activation, forms a hairpin stabilized by Dib1 and Prp8 while the adjacent nucleotides interact with the exon binding loop 1 of U5 snRNA. Snu114 harbours GTP, but its putative catalytic histidine is held away from the \u03b3-phosphate by hydrogen bonding to a tyrosine in the amino-terminal domain of Prp8. Mutation of this histidine to alanine has no detectable effect on yeast growth. The structure provides important new insights into the spliceosome activation process leading to the formation of the catalytic centre. ", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nature16940", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2755485", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2761104", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7590", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "530"
      }
    ], 
    "name": "Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 \u00c5 resolution", 
    "pagination": "298", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "7e2bdd18bd9653ddbdfbf52417c83bf9be5f0f6e3bab2d0794ca0abb1e9af4e7"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "26829225"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nature16940"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1026484180"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nature16940", 
      "https://app.dimensions.ai/details/publication/pub.1026484180"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T16:49", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8669_00000550.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/nature16940"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature16940'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature16940'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature16940'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature16940'


 

This table displays all metadata directly associated to this object as RDF triples.

388 TRIPLES      21 PREDICATES      111 URIs      37 LITERALS      26 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nature16940 schema:about N03c6d96b9c2e43f08fb1ce6fc4fc7438
2 N06722ec5aa164de1a8577d73653e307c
3 N0b2d7b6cbdf042a8bb400119cf7536d2
4 N0f145bc7fec340fa8bec76f706d644bc
5 N38395b3ce2864c75b0f2226bf58f6ee1
6 N42474feb60b84f0db7555a4afe2c257e
7 N7a42a21dec0541b19dd661a15f453d4c
8 N81aac4ceb32e4b30ab884ae58e72ce90
9 N97714db988754cbfade6b1b653196de7
10 Na82ac01feebd4b038c568e96956cbb44
11 Nab67c4bed1da438db7a9eddac54079de
12 Nb28998ed6f204b1fae5bda643225591d
13 Nc2b7dca0ebf84c70bfbf422d0aaf6d90
14 Ncc2dbc2eecf843aa8c877f5163ff090e
15 Nd43004ffdd8743698674a0a6f7944651
16 Nd4a9a2e743ea4bb991831800c3c7f6cf
17 Nda41ae25d69347cfa839ddd1328442c8
18 anzsrc-for:06
19 anzsrc-for:0601
20 schema:author N2c09eaa68af94cabbea07d9d420f6963
21 schema:citation sg:pub.10.1038/nature11843
22 sg:pub.10.1038/nature12734
23 sg:pub.10.1038/nature14548
24 sg:pub.10.1038/nmeth.2115
25 sg:pub.10.1038/nmeth.2472
26 sg:pub.10.1038/nmeth.2727
27 sg:pub.10.1038/nprot.2008.62
28 sg:pub.10.1038/nsb923
29 sg:pub.10.1038/nsmb.2815
30 sg:pub.10.1038/nsmb.2951
31 sg:pub.10.1038/nsmb761
32 https://app.dimensions.ai/details/publication/pub.1074678886
33 https://app.dimensions.ai/details/publication/pub.1074795547
34 https://app.dimensions.ai/details/publication/pub.1074957697
35 https://app.dimensions.ai/details/publication/pub.1082961828
36 https://doi.org/10.1006/jmbi.1998.2183
37 https://doi.org/10.1006/jmbi.1999.3258
38 https://doi.org/10.1006/jmbi.2000.3685
39 https://doi.org/10.1016/0092-8674(92)90149-7
40 https://doi.org/10.1016/j.cell.2014.11.049
41 https://doi.org/10.1016/j.jmb.2011.11.010
42 https://doi.org/10.1016/j.jsb.2006.06.010
43 https://doi.org/10.1016/j.molcel.2006.05.043
44 https://doi.org/10.1016/j.molcel.2009.09.040
45 https://doi.org/10.1016/j.str.2013.04.017
46 https://doi.org/10.1016/j.tibs.2014.10.005
47 https://doi.org/10.1016/j.ultramic.2013.06.004
48 https://doi.org/10.1016/s0960-9822(07)00345-4
49 https://doi.org/10.1016/s1047-8477(03)00069-8
50 https://doi.org/10.1016/s1097-2765(00)80174-4
51 https://doi.org/10.1038/emboj.2009.171
52 https://doi.org/10.1073/pnas.0611591104
53 https://doi.org/10.1073/pnas.1412676111
54 https://doi.org/10.1073/pnas.82.2.488
55 https://doi.org/10.1073/pnas.95.8.4188
56 https://doi.org/10.1074/jbc.m303043200
57 https://doi.org/10.1074/jbc.m505060200
58 https://doi.org/10.1093/emboj/16.13.4092
59 https://doi.org/10.1093/emboj/18.16.4535
60 https://doi.org/10.1093/emboj/cdf544
61 https://doi.org/10.1093/nar/21.7.1555
62 https://doi.org/10.1093/nar/30.4.1029
63 https://doi.org/10.1101/cshperspect.a003707
64 https://doi.org/10.1101/gad.199307.112
65 https://doi.org/10.1107/s0907444910007493
66 https://doi.org/10.1107/s0907444911001314
67 https://doi.org/10.1107/s1399004714016241
68 https://doi.org/10.1107/s1399004714021683
69 https://doi.org/10.1126/science.1137924
70 https://doi.org/10.1126/science.1153803
71 https://doi.org/10.1126/science.1235490
72 https://doi.org/10.1126/science.8266094
73 https://doi.org/10.1126/science.aac7629
74 https://doi.org/10.1126/science.aac8159
75 https://doi.org/10.1146/annurev-biochem-062708-134043
76 https://doi.org/10.1146/annurev.biophys.35.040405.101953
77 https://doi.org/10.1146/annurev.ge.28.120194.000245
78 https://doi.org/10.1261/rna.039024.113
79 https://doi.org/10.1261/rna.040980.113
80 https://doi.org/10.1261/rna.2229706
81 https://doi.org/10.1261/rna.2319806
82 https://doi.org/10.1534/genetics.105.042044
83 https://doi.org/10.1534/genetics.109.107243
84 https://doi.org/10.7554/elife.03665
85 https://doi.org/10.7554/elife.07320
86 https://doi.org/10.7554/elife.11182
87 schema:datePublished 2016-02-01
88 schema:datePublishedReg 2016-02-01
89 schema:description U4/U6.U5 tri-snRNP represents a substantial part of the spliceosome before activation. A cryo-electron microscopy structure of Saccharomyces cerevisiae U4/U6.U5 tri-snRNP at 3.7 Å resolution led to an essentially complete atomic model comprising 30 proteins plus U4/U6 and U5 small nuclear RNAs (snRNAs). The structure reveals striking interweaving interactions of the protein and RNA components, including extended polypeptides penetrating into subunit interfaces. The invariant ACAGAGA sequence of U6 snRNA, which base-pairs with the 5'-splice site during catalytic activation, forms a hairpin stabilized by Dib1 and Prp8 while the adjacent nucleotides interact with the exon binding loop 1 of U5 snRNA. Snu114 harbours GTP, but its putative catalytic histidine is held away from the γ-phosphate by hydrogen bonding to a tyrosine in the amino-terminal domain of Prp8. Mutation of this histidine to alanine has no detectable effect on yeast growth. The structure provides important new insights into the spliceosome activation process leading to the formation of the catalytic centre.
90 schema:genre research_article
91 schema:inLanguage en
92 schema:isAccessibleForFree true
93 schema:isPartOf N0b8c55c6b657411dbc172a9d52079985
94 N3cc57e8d74ce49c58fa933563072f739
95 sg:journal.1018957
96 schema:name Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 Å resolution
97 schema:pagination 298
98 schema:productId N29572c315000431ebd12150fb4c0dd93
99 N2dd23e90043b4d42850b66fb61ab72e7
100 N32971d2782574017a14a8427c634ecc1
101 N82dcfd85ac6845a5bbaae748dc31d1ec
102 Ne1131e9306354978adcb23513bff2943
103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026484180
104 https://doi.org/10.1038/nature16940
105 schema:sdDatePublished 2019-04-10T16:49
106 schema:sdLicense https://scigraph.springernature.com/explorer/license/
107 schema:sdPublisher N12ad517602e44adf943aef9ebc535918
108 schema:url https://www.nature.com/articles/nature16940
109 sgo:license sg:explorer/license/
110 sgo:sdDataset articles
111 rdf:type schema:ScholarlyArticle
112 N02971902f487484cb3fd387920281fcd rdf:first sg:person.01037540344.37
113 rdf:rest N40512a01ba204e4aa259618589273091
114 N03c6d96b9c2e43f08fb1ce6fc4fc7438 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Models, Molecular
116 rdf:type schema:DefinedTerm
117 N06722ec5aa164de1a8577d73653e307c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name RNA, Small Nuclear
119 rdf:type schema:DefinedTerm
120 N0b2d7b6cbdf042a8bb400119cf7536d2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Guanosine Triphosphate
122 rdf:type schema:DefinedTerm
123 N0b8c55c6b657411dbc172a9d52079985 schema:issueNumber 7590
124 rdf:type schema:PublicationIssue
125 N0cc98b9aad854668b13bb3c5460b40b9 rdf:first sg:person.01350114410.45
126 rdf:rest Naf6ba354e85e43fb89659f98d23355b5
127 N0f145bc7fec340fa8bec76f706d644bc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Cryoelectron Microscopy
129 rdf:type schema:DefinedTerm
130 N12ad517602e44adf943aef9ebc535918 schema:name Springer Nature - SN SciGraph project
131 rdf:type schema:Organization
132 N29572c315000431ebd12150fb4c0dd93 schema:name dimensions_id
133 schema:value pub.1026484180
134 rdf:type schema:PropertyValue
135 N2c09eaa68af94cabbea07d9d420f6963 rdf:first sg:person.01123147727.03
136 rdf:rest N02971902f487484cb3fd387920281fcd
137 N2dd23e90043b4d42850b66fb61ab72e7 schema:name doi
138 schema:value 10.1038/nature16940
139 rdf:type schema:PropertyValue
140 N32971d2782574017a14a8427c634ecc1 schema:name nlm_unique_id
141 schema:value 0410462
142 rdf:type schema:PropertyValue
143 N38395b3ce2864c75b0f2226bf58f6ee1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Ribonucleoproteins, Small Nuclear
145 rdf:type schema:DefinedTerm
146 N3cc57e8d74ce49c58fa933563072f739 schema:volumeNumber 530
147 rdf:type schema:PublicationVolume
148 N40512a01ba204e4aa259618589273091 rdf:first sg:person.013775516617.11
149 rdf:rest N0cc98b9aad854668b13bb3c5460b40b9
150 N42474feb60b84f0db7555a4afe2c257e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Exons
152 rdf:type schema:DefinedTerm
153 N5319f3ce0490406589ef1cbe4e7775eb rdf:first sg:person.015537361467.29
154 rdf:rest N74f395ae40de4b03bf5fe50f4b595db3
155 N74f395ae40de4b03bf5fe50f4b595db3 rdf:first sg:person.011244132717.80
156 rdf:rest rdf:nil
157 N7a42a21dec0541b19dd661a15f453d4c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Saccharomyces cerevisiae Proteins
159 rdf:type schema:DefinedTerm
160 N81aac4ceb32e4b30ab884ae58e72ce90 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
161 schema:name Spliceosomes
162 rdf:type schema:DefinedTerm
163 N82dcfd85ac6845a5bbaae748dc31d1ec schema:name pubmed_id
164 schema:value 26829225
165 rdf:type schema:PropertyValue
166 N97714db988754cbfade6b1b653196de7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
167 schema:name Hydrogen Bonding
168 rdf:type schema:DefinedTerm
169 Na82ac01feebd4b038c568e96956cbb44 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Ribonucleoprotein, U5 Small Nuclear
171 rdf:type schema:DefinedTerm
172 Nab67c4bed1da438db7a9eddac54079de schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
173 schema:name Nucleic Acid Conformation
174 rdf:type schema:DefinedTerm
175 Naf6ba354e85e43fb89659f98d23355b5 rdf:first sg:person.016205631512.60
176 rdf:rest N5319f3ce0490406589ef1cbe4e7775eb
177 Nb28998ed6f204b1fae5bda643225591d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
178 schema:name Saccharomyces cerevisiae
179 rdf:type schema:DefinedTerm
180 Nc2b7dca0ebf84c70bfbf422d0aaf6d90 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
181 schema:name DNA Helicases
182 rdf:type schema:DefinedTerm
183 Ncc2dbc2eecf843aa8c877f5163ff090e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
184 schema:name Catalytic Domain
185 rdf:type schema:DefinedTerm
186 Nd43004ffdd8743698674a0a6f7944651 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
187 schema:name RNA Splice Sites
188 rdf:type schema:DefinedTerm
189 Nd4a9a2e743ea4bb991831800c3c7f6cf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
190 schema:name Ribonucleoprotein, U4-U6 Small Nuclear
191 rdf:type schema:DefinedTerm
192 Nda41ae25d69347cfa839ddd1328442c8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
193 schema:name Base Pairing
194 rdf:type schema:DefinedTerm
195 Ne1131e9306354978adcb23513bff2943 schema:name readcube_id
196 schema:value 7e2bdd18bd9653ddbdfbf52417c83bf9be5f0f6e3bab2d0794ca0abb1e9af4e7
197 rdf:type schema:PropertyValue
198 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
199 schema:name Biological Sciences
200 rdf:type schema:DefinedTerm
201 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
202 schema:name Biochemistry and Cell Biology
203 rdf:type schema:DefinedTerm
204 sg:grant.2755485 http://pending.schema.org/fundedItem sg:pub.10.1038/nature16940
205 rdf:type schema:MonetaryGrant
206 sg:grant.2761104 http://pending.schema.org/fundedItem sg:pub.10.1038/nature16940
207 rdf:type schema:MonetaryGrant
208 sg:journal.1018957 schema:issn 0090-0028
209 1476-4687
210 schema:name Nature
211 rdf:type schema:Periodical
212 sg:person.01037540344.37 schema:affiliation https://www.grid.ac/institutes/grid.42475.30
213 schema:familyName Galej
214 schema:givenName Wojciech P.
215 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037540344.37
216 rdf:type schema:Person
217 sg:person.01123147727.03 schema:affiliation https://www.grid.ac/institutes/grid.42475.30
218 schema:familyName Nguyen
219 schema:givenName Thi Hoang Duong
220 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123147727.03
221 rdf:type schema:Person
222 sg:person.011244132717.80 schema:affiliation https://www.grid.ac/institutes/grid.42475.30
223 schema:familyName Nagai
224 schema:givenName Kiyoshi
225 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011244132717.80
226 rdf:type schema:Person
227 sg:person.01350114410.45 schema:affiliation https://www.grid.ac/institutes/grid.42475.30
228 schema:familyName Oubridge
229 schema:givenName Chris
230 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01350114410.45
231 rdf:type schema:Person
232 sg:person.013775516617.11 schema:affiliation https://www.grid.ac/institutes/grid.42475.30
233 schema:familyName Bai
234 schema:givenName Xiao-chen
235 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013775516617.11
236 rdf:type schema:Person
237 sg:person.015537361467.29 schema:affiliation https://www.grid.ac/institutes/grid.42475.30
238 schema:familyName Scheres
239 schema:givenName Sjors H. W.
240 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015537361467.29
241 rdf:type schema:Person
242 sg:person.016205631512.60 schema:affiliation https://www.grid.ac/institutes/grid.42475.30
243 schema:familyName Newman
244 schema:givenName Andrew J.
245 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016205631512.60
246 rdf:type schema:Person
247 sg:pub.10.1038/nature11843 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041916267
248 https://doi.org/10.1038/nature11843
249 rdf:type schema:CreativeWork
250 sg:pub.10.1038/nature12734 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013048897
251 https://doi.org/10.1038/nature12734
252 rdf:type schema:CreativeWork
253 sg:pub.10.1038/nature14548 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040399474
254 https://doi.org/10.1038/nature14548
255 rdf:type schema:CreativeWork
256 sg:pub.10.1038/nmeth.2115 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041024070
257 https://doi.org/10.1038/nmeth.2115
258 rdf:type schema:CreativeWork
259 sg:pub.10.1038/nmeth.2472 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050203089
260 https://doi.org/10.1038/nmeth.2472
261 rdf:type schema:CreativeWork
262 sg:pub.10.1038/nmeth.2727 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009301311
263 https://doi.org/10.1038/nmeth.2727
264 rdf:type schema:CreativeWork
265 sg:pub.10.1038/nprot.2008.62 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046618492
266 https://doi.org/10.1038/nprot.2008.62
267 rdf:type schema:CreativeWork
268 sg:pub.10.1038/nsb923 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011470000
269 https://doi.org/10.1038/nsb923
270 rdf:type schema:CreativeWork
271 sg:pub.10.1038/nsmb.2815 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012432420
272 https://doi.org/10.1038/nsmb.2815
273 rdf:type schema:CreativeWork
274 sg:pub.10.1038/nsmb.2951 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033777572
275 https://doi.org/10.1038/nsmb.2951
276 rdf:type schema:CreativeWork
277 sg:pub.10.1038/nsmb761 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004637296
278 https://doi.org/10.1038/nsmb761
279 rdf:type schema:CreativeWork
280 https://app.dimensions.ai/details/publication/pub.1074678886 schema:CreativeWork
281 https://app.dimensions.ai/details/publication/pub.1074795547 schema:CreativeWork
282 https://app.dimensions.ai/details/publication/pub.1074957697 schema:CreativeWork
283 https://app.dimensions.ai/details/publication/pub.1082961828 schema:CreativeWork
284 https://doi.org/10.1006/jmbi.1998.2183 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019972929
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1006/jmbi.1999.3258 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042635354
287 rdf:type schema:CreativeWork
288 https://doi.org/10.1006/jmbi.2000.3685 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048901077
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1016/0092-8674(92)90149-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023769788
291 rdf:type schema:CreativeWork
292 https://doi.org/10.1016/j.cell.2014.11.049 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008221827
293 rdf:type schema:CreativeWork
294 https://doi.org/10.1016/j.jmb.2011.11.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052958331
295 rdf:type schema:CreativeWork
296 https://doi.org/10.1016/j.jsb.2006.06.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002622528
297 rdf:type schema:CreativeWork
298 https://doi.org/10.1016/j.molcel.2006.05.043 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019947136
299 rdf:type schema:CreativeWork
300 https://doi.org/10.1016/j.molcel.2009.09.040 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023972722
301 rdf:type schema:CreativeWork
302 https://doi.org/10.1016/j.str.2013.04.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035257810
303 rdf:type schema:CreativeWork
304 https://doi.org/10.1016/j.tibs.2014.10.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053316395
305 rdf:type schema:CreativeWork
306 https://doi.org/10.1016/j.ultramic.2013.06.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000304364
307 rdf:type schema:CreativeWork
308 https://doi.org/10.1016/s0960-9822(07)00345-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048841316
309 rdf:type schema:CreativeWork
310 https://doi.org/10.1016/s1047-8477(03)00069-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046234181
311 rdf:type schema:CreativeWork
312 https://doi.org/10.1016/s1097-2765(00)80174-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021674452
313 rdf:type schema:CreativeWork
314 https://doi.org/10.1038/emboj.2009.171 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026584924
315 rdf:type schema:CreativeWork
316 https://doi.org/10.1073/pnas.0611591104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025657900
317 rdf:type schema:CreativeWork
318 https://doi.org/10.1073/pnas.1412676111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009047707
319 rdf:type schema:CreativeWork
320 https://doi.org/10.1073/pnas.82.2.488 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026991187
321 rdf:type schema:CreativeWork
322 https://doi.org/10.1073/pnas.95.8.4188 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025132576
323 rdf:type schema:CreativeWork
324 https://doi.org/10.1074/jbc.m303043200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032916959
325 rdf:type schema:CreativeWork
326 https://doi.org/10.1074/jbc.m505060200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005737822
327 rdf:type schema:CreativeWork
328 https://doi.org/10.1093/emboj/16.13.4092 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016622427
329 rdf:type schema:CreativeWork
330 https://doi.org/10.1093/emboj/18.16.4535 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049683698
331 rdf:type schema:CreativeWork
332 https://doi.org/10.1093/emboj/cdf544 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045460564
333 rdf:type schema:CreativeWork
334 https://doi.org/10.1093/nar/21.7.1555 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003890063
335 rdf:type schema:CreativeWork
336 https://doi.org/10.1093/nar/30.4.1029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050589838
337 rdf:type schema:CreativeWork
338 https://doi.org/10.1101/cshperspect.a003707 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036507675
339 rdf:type schema:CreativeWork
340 https://doi.org/10.1101/gad.199307.112 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022811615
341 rdf:type schema:CreativeWork
342 https://doi.org/10.1107/s0907444910007493 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000235100
343 rdf:type schema:CreativeWork
344 https://doi.org/10.1107/s0907444911001314 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032555428
345 rdf:type schema:CreativeWork
346 https://doi.org/10.1107/s1399004714016241 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030014777
347 rdf:type schema:CreativeWork
348 https://doi.org/10.1107/s1399004714021683 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028026527
349 rdf:type schema:CreativeWork
350 https://doi.org/10.1126/science.1137924 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062455159
351 rdf:type schema:CreativeWork
352 https://doi.org/10.1126/science.1153803 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050940808
353 rdf:type schema:CreativeWork
354 https://doi.org/10.1126/science.1235490 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015444206
355 rdf:type schema:CreativeWork
356 https://doi.org/10.1126/science.8266094 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062653902
357 rdf:type schema:CreativeWork
358 https://doi.org/10.1126/science.aac7629 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043702597
359 rdf:type schema:CreativeWork
360 https://doi.org/10.1126/science.aac8159 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010514152
361 rdf:type schema:CreativeWork
362 https://doi.org/10.1146/annurev-biochem-062708-134043 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034927678
363 rdf:type schema:CreativeWork
364 https://doi.org/10.1146/annurev.biophys.35.040405.101953 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026456216
365 rdf:type schema:CreativeWork
366 https://doi.org/10.1146/annurev.ge.28.120194.000245 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037969108
367 rdf:type schema:CreativeWork
368 https://doi.org/10.1261/rna.039024.113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033721337
369 rdf:type schema:CreativeWork
370 https://doi.org/10.1261/rna.040980.113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010894553
371 rdf:type schema:CreativeWork
372 https://doi.org/10.1261/rna.2229706 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048835685
373 rdf:type schema:CreativeWork
374 https://doi.org/10.1261/rna.2319806 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045305977
375 rdf:type schema:CreativeWork
376 https://doi.org/10.1534/genetics.105.042044 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026799986
377 rdf:type schema:CreativeWork
378 https://doi.org/10.1534/genetics.109.107243 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052339206
379 rdf:type schema:CreativeWork
380 https://doi.org/10.7554/elife.03665 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024693389
381 rdf:type schema:CreativeWork
382 https://doi.org/10.7554/elife.07320 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011907345
383 rdf:type schema:CreativeWork
384 https://doi.org/10.7554/elife.11182 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023050774
385 rdf:type schema:CreativeWork
386 https://www.grid.ac/institutes/grid.42475.30 schema:alternateName MRC Laboratory of Molecular Biology
387 schema:name MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK
388 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...