Reconstructing lineage hierarchies of the distal lung epithelium using single-cell RNA-seq View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2014-05

AUTHORS

Barbara Treutlein, Doug G. Brownfield, Angela R. Wu, Norma F. Neff, Gary L. Mantalas, F. Hernan Espinoza, Tushar J. Desai, Mark A. Krasnow, Stephen R. Quake

ABSTRACT

The mammalian lung is a highly branched network in which the distal regions of the bronchial tree transform during development into a densely packed honeycomb of alveolar air sacs that mediate gas exchange. Although this transformation has been studied by marker expression analysis and fate-mapping, the mechanisms that control the progression of lung progenitors along distinct lineages into mature alveolar cell types are still incompletely known, in part because of the limited number of lineage markers and the effects of ensemble averaging in conventional transcriptome analysis experiments on cell populations. Here we show that single-cell transcriptome analysis circumvents these problems and enables direct measurement of the various cell types and hierarchies in the developing lung. We used microfluidic single-cell RNA sequencing (RNA-seq) on 198 individual cells at four different stages encompassing alveolar differentiation to measure the transcriptional states which define the developmental and cellular hierarchy of the distal mouse lung epithelium. We empirically classified cells into distinct groups by using an unbiased genome-wide approach that did not require a priori knowledge of the underlying cell types or the previous purification of cell populations. The results confirmed the basic outlines of the classical model of epithelial cell-type diversity in the distal lung and led to the discovery of many previously unknown cell-type markers, including transcriptional regulators that discriminate between the different populations. We reconstructed the molecular steps during maturation of bipotential progenitors along both alveolar lineages and elucidated the full life cycle of the alveolar type 2 cell lineage. This single-cell genomics approach is applicable to any developing or mature tissue to robustly delineate molecularly distinct cell types, define progenitors and lineage hierarchies, and identify lineage-specific regulatory factors. More... »

PAGES

371

Journal

TITLE

Nature

ISSUE

7500

VOLUME

509

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nature13173

DOI

http://dx.doi.org/10.1038/nature13173

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1042638061

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24739965


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