DNA interrogation by the CRISPR RNA-guided endonuclease Cas9 View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2014-03

AUTHORS

Samuel H. Sternberg, Sy Redding, Martin Jinek, Eric C. Greene, Jennifer A. Doudna

ABSTRACT

The clustered regularly interspaced short palindromic repeats (CRISPR)-associated enzyme Cas9 is an RNA-guided endonuclease that uses RNA-DNA base-pairing to target foreign DNA in bacteria. Cas9-guide RNA complexes are also effective genome engineering agents in animals and plants. Here we use single-molecule and bulk biochemical experiments to determine how Cas9-RNA interrogates DNA to find specific cleavage sites. We show that both binding and cleavage of DNA by Cas9-RNA require recognition of a short trinucleotide protospacer adjacent motif (PAM). Non-target DNA binding affinity scales with PAM density, and sequences fully complementary to the guide RNA but lacking a nearby PAM are ignored by Cas9-RNA. Competition assays provide evidence that DNA strand separation and RNA-DNA heteroduplex formation initiate at the PAM and proceed directionally towards the distal end of the target sequence. Furthermore, PAM interactions trigger Cas9 catalytic activity. These results reveal how Cas9 uses PAM recognition to quickly identify potential target sites while scanning large DNA molecules, and to regulate scission of double-stranded DNA. More... »

PAGES

62

References to SciGraph publications

  • 2013-08. Heritable genome editing in C. elegans via a CRISPR-Cas9 system in NATURE METHODS
  • 2009-10. Single-molecule imaging of DNA curtains reveals intrinsic energy landscapes for nucleosome deposition in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2010-11. The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA in NATURE
  • 2013-08. Targeted genome modification of crop plants using a CRISPR-Cas system in NATURE BIOTECHNOLOGY
  • 2013-09. CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering in NATURE BIOTECHNOLOGY
  • 2013-09. High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity in NATURE BIOTECHNOLOGY
  • 2013-08. Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9 in NATURE BIOTECHNOLOGY
  • 2010-12. Single-molecule imaging reveals mechanisms of protein disruption by a DNA translocase in NATURE
  • 2010-08. Visualizing one-dimensional diffusion of eukaryotic DNA repair factors along a chromatin lattice in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2013-10. CRISPR RNA–guided activation of endogenous human genes in NATURE METHODS
  • 2010-01. Self versus non-self discrimination during CRISPR RNA-directed immunity in NATURE
  • 2012-02. RNA-guided genetic silencing systems in bacteria and archaea in NATURE
  • 2013-10. RNA-guided gene activation by CRISPR-Cas9–based transcription factors in NATURE METHODS
  • 2011-03. CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III in NATURE
  • 2013-11. Orthogonal Cas9 proteins for RNA-guided gene regulation and editing in NATURE METHODS
  • 2013-08. Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease in NATURE BIOTECHNOLOGY
  • 2013-02. The promoter-search mechanism of Escherichia coli RNA polymerase is dominated by three-dimensional diffusion in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2013-09. DNA targeting specificity of RNA-guided Cas9 nucleases in NATURE BIOTECHNOLOGY
  • 2013-09. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells in NATURE BIOTECHNOLOGY
  • 2013-03. RNA-guided editing of bacterial genomes using CRISPR-Cas systems in NATURE BIOTECHNOLOGY
  • 2005-11. Direct observation of base-pair stepping by RNA polymerase in NATURE
  • 2013-03. Efficient genome editing in zebrafish using a CRISPR-Cas system in NATURE BIOTECHNOLOGY
  • Journal

    TITLE

    Nature

    ISSUE

    7490

    VOLUME

    507

    Related Patents

  • Methods And Compositions For Modifying A Single Stranded Target Nucleic Acid
  • Engineered Crispr-Cas9 Nucleases
  • Using Rna-Guided Foki Nucleases (Rfns) To Increase Specificity For Rna-Guided Genome Editing
  • Use Of Cationic Lipids To Deliver Cas9
  • Methods For Nucleic Acid Editing
  • Compositions And Methods For Cell Targeted Hpv Treatment
  • Cas9 Proteins Including Ligand-Dependent Inteins
  • Engineered Crispr-Cas9 Nucleases With Altered Pam Specificity
  • Compositions And Methods To Treat Latent Viral Infections
  • Methods And Compositions For Rna-Directed Target Dna Modification And For Rna-Directed Modulation Of Transcription
  • Tracking And Manipulating Cellular Rna Via Nuclear Delivery Of Crispr/Cas9
  • Using Truncated Guide Rnas (Tru-Grnas) To Increase Specificity For Rna-Guided Genome Editing
  • Genome Editing For The Treatment Of Huntington's Disease
  • Engineered Crispr-Cas9 Nucleases With Altered Pam Specificity
  • Method For Normalizing A Nucleic Acid Sample
  • Compositions And Methods Of Delivering Treatments For Latent Viral Infections
  • Genome Editing For The Treatment Of Huntington's Disease
  • Engineered Crispr-Cas9 Nucleases With Altered Pam Specificity
  • Crispr-Cas Compositions And Methods
  • Using Rna-Guided Foki Nucleases (Rfns) To Increase Specificity For Rna-Guided Genome Editing
  • Increasing Specificity For Rna-Guided Genome Editing
  • Crispr-Related Methods And Compositions With Governing Grnas
  • Molecular Identification With Sub-Nanometer Localization Accuracy
  • Delivery Of Negatively Charged Proteins Using Cationic Lipids
  • Crispr/Cas-Related Methods And Compositions For Treating Leber's Congenital Amaurosis 10 (Lca10)
  • Programmable Rna Shredding By The Type Iii-A Crispr-Cas System Of Streptococcus Thermophilus
  • Engineered Crispr-Cas9 Nucleases
  • Compositions And Methods To Treat Latent Viral Infections
  • Delivery System For Functional Nucleases
  • Methods And Compositions For Prevention Or Treatment Of A Disease
  • Methods And Compositions For Modifying A Single Stranded Target Nucleic Acid
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nature13011

    DOI

    http://dx.doi.org/10.1038/nature13011

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1037342101

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24476820


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Apoenzymes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Pairing", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Biocatalysis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "CRISPR-Associated Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "CRISPR-Cas Systems", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Clustered Regularly Interspaced Short Palindromic Repeats", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA Cleavage", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Diffusion", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Endonucleases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Enzyme Activation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Engineering", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genome", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Nucleic Acid Denaturation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Nucleic Acid Heteroduplexes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Nucleotide Motifs", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "RNA", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Streptococcus pyogenes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Substrate Specificity", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Thermodynamics", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "University of California, Berkeley", 
              "id": "https://www.grid.ac/institutes/grid.47840.3f", 
              "name": [
                "Department of Chemistry, University of California, Berkeley, California 94720, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sternberg", 
            "givenName": "Samuel H.", 
            "id": "sg:person.01101567113.85", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101567113.85"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Columbia University", 
              "id": "https://www.grid.ac/institutes/grid.21729.3f", 
              "name": [
                "Department of Chemistry, Columbia University, New York, New York 10032, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Redding", 
            "givenName": "Sy", 
            "id": "sg:person.01331164734.02", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01331164734.02"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of California, Berkeley", 
              "id": "https://www.grid.ac/institutes/grid.47840.3f", 
              "name": [
                "Howard Hughes Medical Institute, University of California, Berkeley, California 94720, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jinek", 
            "givenName": "Martin", 
            "id": "sg:person.01270614127.40", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270614127.40"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Columbia University", 
              "id": "https://www.grid.ac/institutes/grid.21729.3f", 
              "name": [
                "Department of Biochemistry and Molecular Biophysics and Howard Hughes Medical Institute, Columbia University, New York, New York 10032, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Greene", 
            "givenName": "Eric C.", 
            "id": "sg:person.0651230655.34", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0651230655.34"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Lawrence Berkeley National Laboratory", 
              "id": "https://www.grid.ac/institutes/grid.184769.5", 
              "name": [
                "Department of Chemistry, University of California, Berkeley, California 94720, USA", 
                "Howard Hughes Medical Institute, University of California, Berkeley, California 94720, USA", 
                "Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA", 
                "Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Doudna", 
            "givenName": "Jennifer A.", 
            "id": "sg:person.01147702313.96", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01147702313.96"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/j.molcel.2012.03.020", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001707425"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkt520", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002402497"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb.2472", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002701823", 
              "https://doi.org/10.1038/nsmb.2472"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1534/genetics.113.152710", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003783977"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1534/genetics.113.152710", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003783977"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/la902443e", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006512784"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/la902443e", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006512784"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.83.6.1608", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006858339"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2673", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007302590", 
              "https://doi.org/10.1038/nbt.2673"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2013.02.022", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008291683"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2623", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009511624", 
              "https://doi.org/10.1038/nbt.2623"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature09523", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009515358", 
              "https://doi.org/10.1038/nature09523"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature09523", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009515358", 
              "https://doi.org/10.1038/nature09523"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb.1858", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009772617", 
              "https://doi.org/10.1038/nsmb.1858"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1104144108", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011358757"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1159689", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011436615"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1208507109", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011954581"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2647", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012543463", 
              "https://doi.org/10.1038/nbt.2647"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/mp/sst119", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013464650"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2013.04.025", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013935649"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev-biochem-060408-091030", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015194503"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1261/rna.030882.111", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015360819"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2013.06.044", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018287305"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1231143", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019873131"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2508", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019888616", 
              "https://doi.org/10.1038/nbt.2508"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1211364109", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021758662"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1232033", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022072971"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.7554/elife.00471", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023633935"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2532", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023792803", 
              "https://doi.org/10.1038/nmeth.2532"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2600", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024354143", 
              "https://doi.org/10.1038/nmeth.2600"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2501", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024988605", 
              "https://doi.org/10.1038/nbt.2501"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb.1655", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027273705", 
              "https://doi.org/10.1038/nsmb.1655"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nsmb.1655", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027273705", 
              "https://doi.org/10.1038/nsmb.1655"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2681", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028304735", 
              "https://doi.org/10.1038/nmeth.2681"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2655", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028628116", 
              "https://doi.org/10.1038/nbt.2655"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature08703", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029569656", 
              "https://doi.org/10.1038/nature08703"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature08703", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029569656", 
              "https://doi.org/10.1038/nature08703"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature09886", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030591890", 
              "https://doi.org/10.1038/nature09886"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature04268", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033521296", 
              "https://doi.org/10.1038/nature04268"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature04268", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033521296", 
              "https://doi.org/10.1038/nature04268"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature04268", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033521296", 
              "https://doi.org/10.1038/nature04268"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1102716108", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034397462"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1099/mic.0.023960-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034551304"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1138140", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036312168"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2650", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040481921", 
              "https://doi.org/10.1038/nbt.2650"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2675", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041659759", 
              "https://doi.org/10.1038/nbt.2675"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1225829", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041850060"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1313587110", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042978468"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.2654", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045833400", 
              "https://doi.org/10.1038/nbt.2654"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.2598", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049500416", 
              "https://doi.org/10.1038/nmeth.2598"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature09561", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049552724", 
              "https://doi.org/10.1038/nature09561"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.celrep.2013.06.020", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051698837"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature10886", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053669571", 
              "https://doi.org/10.1038/nature10886"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/la801762h", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1056163175"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/la801762h", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1056163175"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079159639", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2014-03", 
        "datePublishedReg": "2014-03-01", 
        "description": "The clustered regularly interspaced short palindromic repeats (CRISPR)-associated enzyme Cas9 is an RNA-guided endonuclease that uses RNA-DNA base-pairing to target foreign DNA in bacteria. Cas9-guide RNA complexes are also effective genome engineering agents in animals and plants. Here we use single-molecule and bulk biochemical experiments to determine how Cas9-RNA interrogates DNA to find specific cleavage sites. We show that both binding and cleavage of DNA by Cas9-RNA require recognition of a short trinucleotide protospacer adjacent motif (PAM). Non-target DNA binding affinity scales with PAM density, and sequences fully complementary to the guide RNA but lacking a nearby PAM are ignored by Cas9-RNA. Competition assays provide evidence that DNA strand separation and RNA-DNA heteroduplex formation initiate at the PAM and proceed directionally towards the distal end of the target sequence. Furthermore, PAM interactions trigger Cas9 catalytic activity. These results reveal how Cas9 uses PAM recognition to quickly identify potential target sites while scanning large DNA molecules, and to regulate scission of double-stranded DNA. ", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nature13011", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2518719", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.3001567", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.3480456", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2684074", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2518838", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1018957", 
            "issn": [
              "0090-0028", 
              "1476-4687"
            ], 
            "name": "Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "7490", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "507"
          }
        ], 
        "name": "DNA interrogation by the CRISPR RNA-guided endonuclease Cas9", 
        "pagination": "62", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "b1e231372a436474334cde2e86a10e4ec7491b258485131d12060e55d2a2a57b"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "24476820"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "0410462"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nature13011"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1037342101"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nature13011", 
          "https://app.dimensions.ai/details/publication/pub.1037342101"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T23:31", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8693_00000550.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://www.nature.com/articles/nature13011"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature13011'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature13011'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature13011'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature13011'


     

    This table displays all metadata directly associated to this object as RDF triples.

    367 TRIPLES      21 PREDICATES      98 URIs      42 LITERALS      30 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nature13011 schema:about N02f2cc4302b84380bd3215f7e255c3ba
    2 N06e6c37dccf842288fdce71965c8d9f2
    3 N129db923fac4477494b5395f045b5be7
    4 N1579de8d5e7141e382eaf57ea853f7e1
    5 N1b11ef9f3d84453e81b104a282888367
    6 N1d53daf4093f4974932c105142eec984
    7 N23b43a14229f4c178e03498cba7397b7
    8 N23b59e694e81469bab308c2d2d6ee7d8
    9 N3929da74137645b283085a739b5f4ee3
    10 N4cc3efbb2cab47c7aaeb945278b6945a
    11 N56cc3973b6e44c7aa8d8ef4b1b059d00
    12 N60a0eac6d9e7461683b577bd6491727c
    13 N60bbc0a3f82f4c2e8aa108812dcc32b5
    14 N61e7eec5e0c349738e1b607f9b4fd8fb
    15 N6370cf3936964559accc36e9f718a001
    16 N8354e00babe64aea97639ef5685dd317
    17 N8ea02538027b4711884a45b9841b734d
    18 Nad49c318772544ebbd89e66e413be54f
    19 Nd338d58013ca436296845930a12ccbf4
    20 Ned98f44c8ac04da2ab03293201f1af6c
    21 Nf6a00b80550742f6b5ac9f40e390abc5
    22 anzsrc-for:06
    23 anzsrc-for:0604
    24 schema:author N7f68e6b41fbe4da694d4f0046ad961f1
    25 schema:citation sg:pub.10.1038/nature04268
    26 sg:pub.10.1038/nature08703
    27 sg:pub.10.1038/nature09523
    28 sg:pub.10.1038/nature09561
    29 sg:pub.10.1038/nature09886
    30 sg:pub.10.1038/nature10886
    31 sg:pub.10.1038/nbt.2501
    32 sg:pub.10.1038/nbt.2508
    33 sg:pub.10.1038/nbt.2623
    34 sg:pub.10.1038/nbt.2647
    35 sg:pub.10.1038/nbt.2650
    36 sg:pub.10.1038/nbt.2654
    37 sg:pub.10.1038/nbt.2655
    38 sg:pub.10.1038/nbt.2673
    39 sg:pub.10.1038/nbt.2675
    40 sg:pub.10.1038/nmeth.2532
    41 sg:pub.10.1038/nmeth.2598
    42 sg:pub.10.1038/nmeth.2600
    43 sg:pub.10.1038/nmeth.2681
    44 sg:pub.10.1038/nsmb.1655
    45 sg:pub.10.1038/nsmb.1858
    46 sg:pub.10.1038/nsmb.2472
    47 https://app.dimensions.ai/details/publication/pub.1079159639
    48 https://doi.org/10.1016/j.cell.2013.02.022
    49 https://doi.org/10.1016/j.cell.2013.04.025
    50 https://doi.org/10.1016/j.cell.2013.06.044
    51 https://doi.org/10.1016/j.celrep.2013.06.020
    52 https://doi.org/10.1016/j.molcel.2012.03.020
    53 https://doi.org/10.1021/la801762h
    54 https://doi.org/10.1021/la902443e
    55 https://doi.org/10.1073/pnas.1102716108
    56 https://doi.org/10.1073/pnas.1104144108
    57 https://doi.org/10.1073/pnas.1208507109
    58 https://doi.org/10.1073/pnas.1211364109
    59 https://doi.org/10.1073/pnas.1313587110
    60 https://doi.org/10.1073/pnas.83.6.1608
    61 https://doi.org/10.1093/mp/sst119
    62 https://doi.org/10.1093/nar/gkt520
    63 https://doi.org/10.1099/mic.0.023960-0
    64 https://doi.org/10.1126/science.1138140
    65 https://doi.org/10.1126/science.1159689
    66 https://doi.org/10.1126/science.1225829
    67 https://doi.org/10.1126/science.1231143
    68 https://doi.org/10.1126/science.1232033
    69 https://doi.org/10.1146/annurev-biochem-060408-091030
    70 https://doi.org/10.1261/rna.030882.111
    71 https://doi.org/10.1534/genetics.113.152710
    72 https://doi.org/10.7554/elife.00471
    73 schema:datePublished 2014-03
    74 schema:datePublishedReg 2014-03-01
    75 schema:description The clustered regularly interspaced short palindromic repeats (CRISPR)-associated enzyme Cas9 is an RNA-guided endonuclease that uses RNA-DNA base-pairing to target foreign DNA in bacteria. Cas9-guide RNA complexes are also effective genome engineering agents in animals and plants. Here we use single-molecule and bulk biochemical experiments to determine how Cas9-RNA interrogates DNA to find specific cleavage sites. We show that both binding and cleavage of DNA by Cas9-RNA require recognition of a short trinucleotide protospacer adjacent motif (PAM). Non-target DNA binding affinity scales with PAM density, and sequences fully complementary to the guide RNA but lacking a nearby PAM are ignored by Cas9-RNA. Competition assays provide evidence that DNA strand separation and RNA-DNA heteroduplex formation initiate at the PAM and proceed directionally towards the distal end of the target sequence. Furthermore, PAM interactions trigger Cas9 catalytic activity. These results reveal how Cas9 uses PAM recognition to quickly identify potential target sites while scanning large DNA molecules, and to regulate scission of double-stranded DNA.
    76 schema:genre research_article
    77 schema:inLanguage en
    78 schema:isAccessibleForFree true
    79 schema:isPartOf N43705afe6ec5465eab90788126c33913
    80 N624f32f56a45447eb67d51fbb052ff96
    81 sg:journal.1018957
    82 schema:name DNA interrogation by the CRISPR RNA-guided endonuclease Cas9
    83 schema:pagination 62
    84 schema:productId N71f4345115374b8ab3e26477d92fa798
    85 N7dd581857d694f4c805bc2dd2fe72984
    86 N8d7b78d1dd9a4f40b6e038ad5431cce4
    87 N99a95a94d2e348ea84f5a405d22f3e11
    88 Neb6410e383684b8f81f07a4ab7ad6a9c
    89 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037342101
    90 https://doi.org/10.1038/nature13011
    91 schema:sdDatePublished 2019-04-10T23:31
    92 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    93 schema:sdPublisher N4f956e4b5b844c5280e22384c599c26d
    94 schema:url https://www.nature.com/articles/nature13011
    95 sgo:license sg:explorer/license/
    96 sgo:sdDataset articles
    97 rdf:type schema:ScholarlyArticle
    98 N02f2cc4302b84380bd3215f7e255c3ba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    99 schema:name CRISPR-Cas Systems
    100 rdf:type schema:DefinedTerm
    101 N06e6c37dccf842288fdce71965c8d9f2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name DNA
    103 rdf:type schema:DefinedTerm
    104 N129db923fac4477494b5395f045b5be7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    105 schema:name Apoenzymes
    106 rdf:type schema:DefinedTerm
    107 N1579de8d5e7141e382eaf57ea853f7e1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    108 schema:name Nucleotide Motifs
    109 rdf:type schema:DefinedTerm
    110 N1b11ef9f3d84453e81b104a282888367 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    111 schema:name Thermodynamics
    112 rdf:type schema:DefinedTerm
    113 N1d53daf4093f4974932c105142eec984 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    114 schema:name Nucleic Acid Heteroduplexes
    115 rdf:type schema:DefinedTerm
    116 N23b43a14229f4c178e03498cba7397b7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    117 schema:name Streptococcus pyogenes
    118 rdf:type schema:DefinedTerm
    119 N23b59e694e81469bab308c2d2d6ee7d8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    120 schema:name Diffusion
    121 rdf:type schema:DefinedTerm
    122 N3929da74137645b283085a739b5f4ee3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    123 schema:name Nucleic Acid Denaturation
    124 rdf:type schema:DefinedTerm
    125 N43705afe6ec5465eab90788126c33913 schema:issueNumber 7490
    126 rdf:type schema:PublicationIssue
    127 N4cc3efbb2cab47c7aaeb945278b6945a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    128 schema:name Biocatalysis
    129 rdf:type schema:DefinedTerm
    130 N4f956e4b5b844c5280e22384c599c26d schema:name Springer Nature - SN SciGraph project
    131 rdf:type schema:Organization
    132 N56cc3973b6e44c7aa8d8ef4b1b059d00 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    133 schema:name Endonucleases
    134 rdf:type schema:DefinedTerm
    135 N60a0eac6d9e7461683b577bd6491727c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    136 schema:name DNA Cleavage
    137 rdf:type schema:DefinedTerm
    138 N60bbc0a3f82f4c2e8aa108812dcc32b5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    139 schema:name Base Sequence
    140 rdf:type schema:DefinedTerm
    141 N61e7eec5e0c349738e1b607f9b4fd8fb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    142 schema:name Base Pairing
    143 rdf:type schema:DefinedTerm
    144 N624f32f56a45447eb67d51fbb052ff96 schema:volumeNumber 507
    145 rdf:type schema:PublicationVolume
    146 N633b5929ff0c46e997ef658a6db3a1ec rdf:first sg:person.01331164734.02
    147 rdf:rest Ndce4d75849f543369126259aa6749006
    148 N6370cf3936964559accc36e9f718a001 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    149 schema:name RNA
    150 rdf:type schema:DefinedTerm
    151 N71f4345115374b8ab3e26477d92fa798 schema:name nlm_unique_id
    152 schema:value 0410462
    153 rdf:type schema:PropertyValue
    154 N72a6323dd15444d8911cce6fc996a34a rdf:first sg:person.01147702313.96
    155 rdf:rest rdf:nil
    156 N7dd581857d694f4c805bc2dd2fe72984 schema:name dimensions_id
    157 schema:value pub.1037342101
    158 rdf:type schema:PropertyValue
    159 N7f68e6b41fbe4da694d4f0046ad961f1 rdf:first sg:person.01101567113.85
    160 rdf:rest N633b5929ff0c46e997ef658a6db3a1ec
    161 N8354e00babe64aea97639ef5685dd317 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    162 schema:name CRISPR-Associated Proteins
    163 rdf:type schema:DefinedTerm
    164 N8d7b78d1dd9a4f40b6e038ad5431cce4 schema:name pubmed_id
    165 schema:value 24476820
    166 rdf:type schema:PropertyValue
    167 N8ea02538027b4711884a45b9841b734d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    168 schema:name Substrate Specificity
    169 rdf:type schema:DefinedTerm
    170 N99a95a94d2e348ea84f5a405d22f3e11 schema:name doi
    171 schema:value 10.1038/nature13011
    172 rdf:type schema:PropertyValue
    173 Nad49c318772544ebbd89e66e413be54f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    174 schema:name Enzyme Activation
    175 rdf:type schema:DefinedTerm
    176 Nd338d58013ca436296845930a12ccbf4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    177 schema:name Genetic Engineering
    178 rdf:type schema:DefinedTerm
    179 Ndce4d75849f543369126259aa6749006 rdf:first sg:person.01270614127.40
    180 rdf:rest Nefdf4033fdc24b8ea807d57aa52ff6c6
    181 Neb6410e383684b8f81f07a4ab7ad6a9c schema:name readcube_id
    182 schema:value b1e231372a436474334cde2e86a10e4ec7491b258485131d12060e55d2a2a57b
    183 rdf:type schema:PropertyValue
    184 Ned98f44c8ac04da2ab03293201f1af6c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    185 schema:name Genome
    186 rdf:type schema:DefinedTerm
    187 Nefdf4033fdc24b8ea807d57aa52ff6c6 rdf:first sg:person.0651230655.34
    188 rdf:rest N72a6323dd15444d8911cce6fc996a34a
    189 Nf6a00b80550742f6b5ac9f40e390abc5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    190 schema:name Clustered Regularly Interspaced Short Palindromic Repeats
    191 rdf:type schema:DefinedTerm
    192 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    193 schema:name Biological Sciences
    194 rdf:type schema:DefinedTerm
    195 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    196 schema:name Genetics
    197 rdf:type schema:DefinedTerm
    198 sg:grant.2518719 http://pending.schema.org/fundedItem sg:pub.10.1038/nature13011
    199 rdf:type schema:MonetaryGrant
    200 sg:grant.2518838 http://pending.schema.org/fundedItem sg:pub.10.1038/nature13011
    201 rdf:type schema:MonetaryGrant
    202 sg:grant.2684074 http://pending.schema.org/fundedItem sg:pub.10.1038/nature13011
    203 rdf:type schema:MonetaryGrant
    204 sg:grant.3001567 http://pending.schema.org/fundedItem sg:pub.10.1038/nature13011
    205 rdf:type schema:MonetaryGrant
    206 sg:grant.3480456 http://pending.schema.org/fundedItem sg:pub.10.1038/nature13011
    207 rdf:type schema:MonetaryGrant
    208 sg:journal.1018957 schema:issn 0090-0028
    209 1476-4687
    210 schema:name Nature
    211 rdf:type schema:Periodical
    212 sg:person.01101567113.85 schema:affiliation https://www.grid.ac/institutes/grid.47840.3f
    213 schema:familyName Sternberg
    214 schema:givenName Samuel H.
    215 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101567113.85
    216 rdf:type schema:Person
    217 sg:person.01147702313.96 schema:affiliation https://www.grid.ac/institutes/grid.184769.5
    218 schema:familyName Doudna
    219 schema:givenName Jennifer A.
    220 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01147702313.96
    221 rdf:type schema:Person
    222 sg:person.01270614127.40 schema:affiliation https://www.grid.ac/institutes/grid.47840.3f
    223 schema:familyName Jinek
    224 schema:givenName Martin
    225 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270614127.40
    226 rdf:type schema:Person
    227 sg:person.01331164734.02 schema:affiliation https://www.grid.ac/institutes/grid.21729.3f
    228 schema:familyName Redding
    229 schema:givenName Sy
    230 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01331164734.02
    231 rdf:type schema:Person
    232 sg:person.0651230655.34 schema:affiliation https://www.grid.ac/institutes/grid.21729.3f
    233 schema:familyName Greene
    234 schema:givenName Eric C.
    235 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0651230655.34
    236 rdf:type schema:Person
    237 sg:pub.10.1038/nature04268 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033521296
    238 https://doi.org/10.1038/nature04268
    239 rdf:type schema:CreativeWork
    240 sg:pub.10.1038/nature08703 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029569656
    241 https://doi.org/10.1038/nature08703
    242 rdf:type schema:CreativeWork
    243 sg:pub.10.1038/nature09523 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009515358
    244 https://doi.org/10.1038/nature09523
    245 rdf:type schema:CreativeWork
    246 sg:pub.10.1038/nature09561 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049552724
    247 https://doi.org/10.1038/nature09561
    248 rdf:type schema:CreativeWork
    249 sg:pub.10.1038/nature09886 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030591890
    250 https://doi.org/10.1038/nature09886
    251 rdf:type schema:CreativeWork
    252 sg:pub.10.1038/nature10886 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053669571
    253 https://doi.org/10.1038/nature10886
    254 rdf:type schema:CreativeWork
    255 sg:pub.10.1038/nbt.2501 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024988605
    256 https://doi.org/10.1038/nbt.2501
    257 rdf:type schema:CreativeWork
    258 sg:pub.10.1038/nbt.2508 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019888616
    259 https://doi.org/10.1038/nbt.2508
    260 rdf:type schema:CreativeWork
    261 sg:pub.10.1038/nbt.2623 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009511624
    262 https://doi.org/10.1038/nbt.2623
    263 rdf:type schema:CreativeWork
    264 sg:pub.10.1038/nbt.2647 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012543463
    265 https://doi.org/10.1038/nbt.2647
    266 rdf:type schema:CreativeWork
    267 sg:pub.10.1038/nbt.2650 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040481921
    268 https://doi.org/10.1038/nbt.2650
    269 rdf:type schema:CreativeWork
    270 sg:pub.10.1038/nbt.2654 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045833400
    271 https://doi.org/10.1038/nbt.2654
    272 rdf:type schema:CreativeWork
    273 sg:pub.10.1038/nbt.2655 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028628116
    274 https://doi.org/10.1038/nbt.2655
    275 rdf:type schema:CreativeWork
    276 sg:pub.10.1038/nbt.2673 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007302590
    277 https://doi.org/10.1038/nbt.2673
    278 rdf:type schema:CreativeWork
    279 sg:pub.10.1038/nbt.2675 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041659759
    280 https://doi.org/10.1038/nbt.2675
    281 rdf:type schema:CreativeWork
    282 sg:pub.10.1038/nmeth.2532 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023792803
    283 https://doi.org/10.1038/nmeth.2532
    284 rdf:type schema:CreativeWork
    285 sg:pub.10.1038/nmeth.2598 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049500416
    286 https://doi.org/10.1038/nmeth.2598
    287 rdf:type schema:CreativeWork
    288 sg:pub.10.1038/nmeth.2600 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024354143
    289 https://doi.org/10.1038/nmeth.2600
    290 rdf:type schema:CreativeWork
    291 sg:pub.10.1038/nmeth.2681 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028304735
    292 https://doi.org/10.1038/nmeth.2681
    293 rdf:type schema:CreativeWork
    294 sg:pub.10.1038/nsmb.1655 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027273705
    295 https://doi.org/10.1038/nsmb.1655
    296 rdf:type schema:CreativeWork
    297 sg:pub.10.1038/nsmb.1858 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009772617
    298 https://doi.org/10.1038/nsmb.1858
    299 rdf:type schema:CreativeWork
    300 sg:pub.10.1038/nsmb.2472 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002701823
    301 https://doi.org/10.1038/nsmb.2472
    302 rdf:type schema:CreativeWork
    303 https://app.dimensions.ai/details/publication/pub.1079159639 schema:CreativeWork
    304 https://doi.org/10.1016/j.cell.2013.02.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008291683
    305 rdf:type schema:CreativeWork
    306 https://doi.org/10.1016/j.cell.2013.04.025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013935649
    307 rdf:type schema:CreativeWork
    308 https://doi.org/10.1016/j.cell.2013.06.044 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018287305
    309 rdf:type schema:CreativeWork
    310 https://doi.org/10.1016/j.celrep.2013.06.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051698837
    311 rdf:type schema:CreativeWork
    312 https://doi.org/10.1016/j.molcel.2012.03.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001707425
    313 rdf:type schema:CreativeWork
    314 https://doi.org/10.1021/la801762h schema:sameAs https://app.dimensions.ai/details/publication/pub.1056163175
    315 rdf:type schema:CreativeWork
    316 https://doi.org/10.1021/la902443e schema:sameAs https://app.dimensions.ai/details/publication/pub.1006512784
    317 rdf:type schema:CreativeWork
    318 https://doi.org/10.1073/pnas.1102716108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034397462
    319 rdf:type schema:CreativeWork
    320 https://doi.org/10.1073/pnas.1104144108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011358757
    321 rdf:type schema:CreativeWork
    322 https://doi.org/10.1073/pnas.1208507109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011954581
    323 rdf:type schema:CreativeWork
    324 https://doi.org/10.1073/pnas.1211364109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021758662
    325 rdf:type schema:CreativeWork
    326 https://doi.org/10.1073/pnas.1313587110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042978468
    327 rdf:type schema:CreativeWork
    328 https://doi.org/10.1073/pnas.83.6.1608 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006858339
    329 rdf:type schema:CreativeWork
    330 https://doi.org/10.1093/mp/sst119 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013464650
    331 rdf:type schema:CreativeWork
    332 https://doi.org/10.1093/nar/gkt520 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002402497
    333 rdf:type schema:CreativeWork
    334 https://doi.org/10.1099/mic.0.023960-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034551304
    335 rdf:type schema:CreativeWork
    336 https://doi.org/10.1126/science.1138140 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036312168
    337 rdf:type schema:CreativeWork
    338 https://doi.org/10.1126/science.1159689 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011436615
    339 rdf:type schema:CreativeWork
    340 https://doi.org/10.1126/science.1225829 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041850060
    341 rdf:type schema:CreativeWork
    342 https://doi.org/10.1126/science.1231143 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019873131
    343 rdf:type schema:CreativeWork
    344 https://doi.org/10.1126/science.1232033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022072971
    345 rdf:type schema:CreativeWork
    346 https://doi.org/10.1146/annurev-biochem-060408-091030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015194503
    347 rdf:type schema:CreativeWork
    348 https://doi.org/10.1261/rna.030882.111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015360819
    349 rdf:type schema:CreativeWork
    350 https://doi.org/10.1534/genetics.113.152710 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003783977
    351 rdf:type schema:CreativeWork
    352 https://doi.org/10.7554/elife.00471 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023633935
    353 rdf:type schema:CreativeWork
    354 https://www.grid.ac/institutes/grid.184769.5 schema:alternateName Lawrence Berkeley National Laboratory
    355 schema:name Department of Chemistry, University of California, Berkeley, California 94720, USA
    356 Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    357 Howard Hughes Medical Institute, University of California, Berkeley, California 94720, USA
    358 Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    359 rdf:type schema:Organization
    360 https://www.grid.ac/institutes/grid.21729.3f schema:alternateName Columbia University
    361 schema:name Department of Biochemistry and Molecular Biophysics and Howard Hughes Medical Institute, Columbia University, New York, New York 10032, USA
    362 Department of Chemistry, Columbia University, New York, New York 10032, USA
    363 rdf:type schema:Organization
    364 https://www.grid.ac/institutes/grid.47840.3f schema:alternateName University of California, Berkeley
    365 schema:name Department of Chemistry, University of California, Berkeley, California 94720, USA
    366 Howard Hughes Medical Institute, University of California, Berkeley, California 94720, USA
    367 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...