DNA-guided DNA interference by a prokaryotic Argonaute View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2014-02-16

AUTHORS

Daan C. Swarts, Matthijs M. Jore, Edze R. Westra, Yifan Zhu, Jorijn H. Janssen, Ambrosius P. Snijders, Yanli Wang, Dinshaw J. Patel, José Berenguer, Stan J. J. Brouns, John van der Oost

ABSTRACT

RNA interference is widely distributed in eukaryotes and has a variety of functions, including antiviral defence and gene regulation. All RNA interference pathways use small single-stranded RNA (ssRNA) molecules that guide proteins of the Argonaute (Ago) family to complementary ssRNA targets: RNA-guided RNA interference. The role of prokaryotic Ago variants has remained elusive, although bioinformatics analysis has suggested their involvement in host defence. Here we demonstrate that Ago of the bacterium Thermus thermophilus (TtAgo) acts as a barrier for the uptake and propagation of foreign DNA. In vivo, TtAgo is loaded with 5'-phosphorylated DNA guides, 13-25 nucleotides in length, that are mostly plasmid derived and have a strong bias for a 5'-end deoxycytidine. These small interfering DNAs guide TtAgo to cleave complementary DNA strands. Hence, despite structural homology to its eukaryotic counterparts, TtAgo functions in host defence by DNA-guided DNA interference. More... »

PAGES

258

Journal

TITLE

Nature

ISSUE

7491

VOLUME

507

Related Patents

  • Methods For Generating Barcoded Combinatorial Libraries
  • Methods And Compositions For Treatment Of A Beta Thalessemia
  • Engineering Animal Or Plant Genome Using Dna-Guided Argonaute Interference Systems (Dais) From Mesophilic Prokaryotes
  • Nuclease-Mediated Regulation Of Gene Expression
  • Methods And Compositions For Regulation Of Zinc Finger Protein Expression
  • Gene Correction Of Scid-Related Genes In Hematopoietic Stem And Progenitor Cells
  • Method Of Integrating Donor Dna Into Target Dna
  • Cas9 Proteins Including Ligand-Dependent Inteins
  • Crispr Enabled Multiplexed Genome Engineering
  • Methods Of Using Oligonucleotide-Guided Argonaute Proteins
  • Methods And Compositions For Modulating Nuclease-Mediated Genome Engineering In Hematopoietic Stem Cells
  • Methods And Compositions For Treating Hemophilia
  • Delivery Methods And Compositions For Nuclease-Mediated Genome Engineering
  • Nucleic Acid-Guided Nucleases
  • Methods Of Modifying A Target Nucleic Acid With An Argonaute
  • Methods And Compositions For Genome Engineering
  • Gene Correction Of Scid-Related Genes In Hematopoietic Stem And Progenitor Cells
  • Dna-Guided Dna Interference By A Prokaryotic Argonaute
  • Methods And Compositions For Using Argonaute To Modify A Single Stranded Target Nucleic Acid
  • Methods Of Using Oligonucleotide-Guided Argonaute Proteins
  • Gene Editing For Hiv Gene Therapy
  • Compositions For Linking Dna-Binding Domains And Cleavage Domains
  • Engineering Mammalian Genome Using Dna-Guided Argonaute Interference Systems (Dais)
  • Delivery Of Negatively Charged Proteins Using Cationic Lipids
  • Nucleic Acid-Guided Nucleases
  • Compositions And Methods Of Nucleic Acid-Targeting Nucleic Acids
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nature12971

    DOI

    http://dx.doi.org/10.1038/nature12971

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1052410148

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24531762


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Argonaute Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Pairing", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA Cleavage", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Deoxycytidine", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Silencing", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Phosphorylation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plasmids", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Prokaryotic Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Thermus thermophilus", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, the Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Swarts", 
            "givenName": "Daan C.", 
            "id": "sg:person.01260020427.57", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01260020427.57"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, the Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jore", 
            "givenName": "Matthijs M.", 
            "id": "sg:person.0747346652.58", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0747346652.58"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, the Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Westra", 
            "givenName": "Edze R.", 
            "id": "sg:person.0710321253.01", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0710321253.01"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, the Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhu", 
            "givenName": "Yifan", 
            "id": "sg:person.0755330446.22", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0755330446.22"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, the Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Janssen", 
            "givenName": "Jorijn H.", 
            "id": "sg:person.01135023106.43", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01135023106.43"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Clare Hall Laboratories, Cancer Research UK, London Research Institute, South Mimms EN6 3LD, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Snijders", 
            "givenName": "Ambrosius P.", 
            "id": "sg:person.01341405243.01", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01341405243.01"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute of Biophysics", 
              "id": "https://www.grid.ac/institutes/grid.418856.6", 
              "name": [
                "Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wang", 
            "givenName": "Yanli", 
            "id": "sg:person.01127537626.16", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01127537626.16"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Memorial Sloan Kettering Cancer Center", 
              "id": "https://www.grid.ac/institutes/grid.51462.34", 
              "name": [
                "Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10065, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Patel", 
            "givenName": "Dinshaw J.", 
            "id": "sg:person.016225305307.50", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016225305307.50"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Autonomous University of Madrid", 
              "id": "https://www.grid.ac/institutes/grid.5515.4", 
              "name": [
                "Centro de Biolog\u00eda Molecular Severo Ochoa, UAM-CSIC, Campus de Cantoblanco, 28049 Madrid, Spain"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Berenguer", 
            "givenName": "Jos\u00e9", 
            "id": "sg:person.012377366202.40", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012377366202.40"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, the Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Brouns", 
            "givenName": "Stan J. J.", 
            "id": "sg:person.0736732301.90", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0736732301.90"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wageningen University & Research", 
              "id": "https://www.grid.ac/institutes/grid.4818.5", 
              "name": [
                "Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, the Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "van der Oost", 
            "givenName": "John", 
            "id": "sg:person.01321677476.16", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321677476.16"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nrmicro1088", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002249031", 
              "https://doi.org/10.1038/nrmicro1088"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrmicro1088", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002249031", 
              "https://doi.org/10.1038/nrmicro1088"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrmicro1088", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002249031", 
              "https://doi.org/10.1038/nrmicro1088"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/emboj/20.23.6877", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004775429"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1038/emboj.2012.204", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004918093"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-2958.2007.05687.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005339956"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/cshperspect.a003772", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010792735"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0168-9525(02)02690-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011065957"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molcel.2005.07.011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015155076"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1745-6150-4-29", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015797830", 
              "https://doi.org/10.1186/1745-6150-4-29"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/21.3.463", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016360203"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1574-6976.2008.00160.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017595065"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1102513", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018006760"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1321032111", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019838218"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature07315", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020271916", 
              "https://doi.org/10.1038/nature07315"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature03514", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021329082", 
              "https://doi.org/10.1038/nature03514"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature03514", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021329082", 
              "https://doi.org/10.1038/nature03514"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature09039", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022404163", 
              "https://doi.org/10.1038/nature09039"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature09039", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022404163", 
              "https://doi.org/10.1038/nature09039"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/bib/bbs012", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022817326"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/bioinformatics/btp352", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023014918"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molcel.2012.03.018", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024792403"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature07666", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029382170", 
              "https://doi.org/10.1038/nature07666"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1038/sj.emboj.7601094", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029981632"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1574-6968.2008.01389.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035594214"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0020425", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035941706"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature11211", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037387037", 
              "https://doi.org/10.1038/nature11211"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1574-6968.1988.tb13940.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040837810"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1574-6968.1988.tb13940.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040837810"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-1-4419-7823-3_1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041025541", 
              "https://doi.org/10.1007/978-1-4419-7823-3_1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-1-4419-7823-3_1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041025541", 
              "https://doi.org/10.1007/978-1-4419-7823-3_1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.molcel.2013.08.014", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041420574"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature08434", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042379011", 
              "https://doi.org/10.1038/nature08434"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature08434", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042379011", 
              "https://doi.org/10.1038/nature08434"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1462-2920.2007.01482.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051181196"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jb.166.1.338-340.1986", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062714841"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jb.176.5.1251-1259.1994", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062723464"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2014-02-16", 
        "datePublishedReg": "2014-02-16", 
        "description": "RNA interference is widely distributed in eukaryotes and has a variety of functions, including antiviral defence and gene regulation. All RNA interference pathways use small single-stranded RNA (ssRNA) molecules that guide proteins of the Argonaute (Ago) family to complementary ssRNA targets: RNA-guided RNA interference. The role of prokaryotic Ago variants has remained elusive, although bioinformatics analysis has suggested their involvement in host defence. Here we demonstrate that Ago of the bacterium Thermus thermophilus (TtAgo) acts as a barrier for the uptake and propagation of foreign DNA. In vivo, TtAgo is loaded with 5'-phosphorylated DNA guides, 13-25 nucleotides in length, that are mostly plasmid derived and have a strong bias for a 5'-end deoxycytidine. These small interfering DNAs guide TtAgo to cleave complementary DNA strands. Hence, despite structural homology to its eukaryotic counterparts, TtAgo functions in host defence by DNA-guided DNA interference. ", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nature12971", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2438793", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.4699840", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.4698598", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2521976", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1018957", 
            "issn": [
              "0090-0028", 
              "1476-4687"
            ], 
            "name": "Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "7491", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "507"
          }
        ], 
        "name": "DNA-guided DNA interference by a prokaryotic Argonaute", 
        "pagination": "258", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "f9a57e24eece9d743a5c566cf854ee434d6c2c4e4f8441e283817c9a2d8d62e9"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "24531762"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "0410462"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nature12971"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1052410148"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nature12971", 
          "https://app.dimensions.ai/details/publication/pub.1052410148"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T21:25", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8687_00000426.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://www.nature.com/articles/nature12971"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature12971'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature12971'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature12971'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature12971'


     

    This table displays all metadata directly associated to this object as RDF triples.

    301 TRIPLES      21 PREDICATES      69 URIs      31 LITERALS      20 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nature12971 schema:about N0137db05516b49a89c1204e924411b06
    2 N08549b96eca246d98b205bb2e997a847
    3 N0da0a991a07b49db9a618220473af76e
    4 N27211189a5694fa191e091ee3e2bfb86
    5 N5a5146cfa57e4b22b510951cab67cf0b
    6 N726479d2d0c846768271801d7618a794
    7 N8926e7d52ebb4fcea1f424dc8beb1946
    8 Nab2610c23cdc43df990174350c5e1042
    9 Naeff6c7d31ca4507ad1b39362cd856f4
    10 Naf6c516fced7426c82083317af08358b
    11 Nc38d0efcfe904d5c892021d6301f487f
    12 anzsrc-for:06
    13 anzsrc-for:0604
    14 schema:author Nac1a98f3ca69492798b36bd4e0fc2034
    15 schema:citation sg:pub.10.1007/978-1-4419-7823-3_1
    16 sg:pub.10.1038/nature03514
    17 sg:pub.10.1038/nature07315
    18 sg:pub.10.1038/nature07666
    19 sg:pub.10.1038/nature08434
    20 sg:pub.10.1038/nature09039
    21 sg:pub.10.1038/nature11211
    22 sg:pub.10.1038/nrmicro1088
    23 sg:pub.10.1186/1745-6150-4-29
    24 https://doi.org/10.1016/j.molcel.2005.07.011
    25 https://doi.org/10.1016/j.molcel.2012.03.018
    26 https://doi.org/10.1016/j.molcel.2013.08.014
    27 https://doi.org/10.1016/s0168-9525(02)02690-2
    28 https://doi.org/10.1038/emboj.2012.204
    29 https://doi.org/10.1038/sj.emboj.7601094
    30 https://doi.org/10.1073/pnas.1321032111
    31 https://doi.org/10.1093/bib/bbs012
    32 https://doi.org/10.1093/bioinformatics/btp352
    33 https://doi.org/10.1093/emboj/20.23.6877
    34 https://doi.org/10.1093/nar/21.3.463
    35 https://doi.org/10.1101/cshperspect.a003772
    36 https://doi.org/10.1111/j.1365-2958.2007.05687.x
    37 https://doi.org/10.1111/j.1462-2920.2007.01482.x
    38 https://doi.org/10.1111/j.1574-6968.1988.tb13940.x
    39 https://doi.org/10.1111/j.1574-6968.2008.01389.x
    40 https://doi.org/10.1111/j.1574-6976.2008.00160.x
    41 https://doi.org/10.1126/science.1102513
    42 https://doi.org/10.1128/jb.166.1.338-340.1986
    43 https://doi.org/10.1128/jb.176.5.1251-1259.1994
    44 https://doi.org/10.1371/journal.pone.0020425
    45 schema:datePublished 2014-02-16
    46 schema:datePublishedReg 2014-02-16
    47 schema:description RNA interference is widely distributed in eukaryotes and has a variety of functions, including antiviral defence and gene regulation. All RNA interference pathways use small single-stranded RNA (ssRNA) molecules that guide proteins of the Argonaute (Ago) family to complementary ssRNA targets: RNA-guided RNA interference. The role of prokaryotic Ago variants has remained elusive, although bioinformatics analysis has suggested their involvement in host defence. Here we demonstrate that Ago of the bacterium Thermus thermophilus (TtAgo) acts as a barrier for the uptake and propagation of foreign DNA. In vivo, TtAgo is loaded with 5'-phosphorylated DNA guides, 13-25 nucleotides in length, that are mostly plasmid derived and have a strong bias for a 5'-end deoxycytidine. These small interfering DNAs guide TtAgo to cleave complementary DNA strands. Hence, despite structural homology to its eukaryotic counterparts, TtAgo functions in host defence by DNA-guided DNA interference.
    48 schema:genre research_article
    49 schema:inLanguage en
    50 schema:isAccessibleForFree true
    51 schema:isPartOf N9cd385b0c8ee473795b8c5fde75ea102
    52 Nf5ca5cc5efc446d5b0602d04b1527f6f
    53 sg:journal.1018957
    54 schema:name DNA-guided DNA interference by a prokaryotic Argonaute
    55 schema:pagination 258
    56 schema:productId N06019cb774ca40b08bbd61bdc027cfd9
    57 N69a68dc46b3d4c8496b90c697594e8e6
    58 N84b2ce8f766347beb4bc5162957b526c
    59 N8fca0d7cde5e467e926eacf326c29bc2
    60 Ncecd3fff50904a4a8ee3b36132240418
    61 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052410148
    62 https://doi.org/10.1038/nature12971
    63 schema:sdDatePublished 2019-04-10T21:25
    64 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    65 schema:sdPublisher N52f12cf8a32849e7afd7d0e4d6364eef
    66 schema:url https://www.nature.com/articles/nature12971
    67 sgo:license sg:explorer/license/
    68 sgo:sdDataset articles
    69 rdf:type schema:ScholarlyArticle
    70 N0137db05516b49a89c1204e924411b06 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    71 schema:name DNA
    72 rdf:type schema:DefinedTerm
    73 N06019cb774ca40b08bbd61bdc027cfd9 schema:name nlm_unique_id
    74 schema:value 0410462
    75 rdf:type schema:PropertyValue
    76 N08549b96eca246d98b205bb2e997a847 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    77 schema:name DNA Cleavage
    78 rdf:type schema:DefinedTerm
    79 N0da0a991a07b49db9a618220473af76e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    80 schema:name Base Sequence
    81 rdf:type schema:DefinedTerm
    82 N27211189a5694fa191e091ee3e2bfb86 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    83 schema:name Thermus thermophilus
    84 rdf:type schema:DefinedTerm
    85 N334c2b495f164f7198a57fe6fe467339 rdf:first sg:person.0710321253.01
    86 rdf:rest Nf0aaee7df496428bb938135a36a01b99
    87 N3d3b88af892144d0bde57a952b7501b6 rdf:first sg:person.01321677476.16
    88 rdf:rest rdf:nil
    89 N4e21f74f77374430ad611bcf9c04a154 rdf:first sg:person.01135023106.43
    90 rdf:rest Nd4e357c520e5436fb350f627526eec8b
    91 N52f12cf8a32849e7afd7d0e4d6364eef schema:name Springer Nature - SN SciGraph project
    92 rdf:type schema:Organization
    93 N538b623d52c04fda8dff4621afd57c72 rdf:first sg:person.016225305307.50
    94 rdf:rest Nce8c5d5adfa648989d1af749a8c414bc
    95 N5a5146cfa57e4b22b510951cab67cf0b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    96 schema:name Argonaute Proteins
    97 rdf:type schema:DefinedTerm
    98 N69a68dc46b3d4c8496b90c697594e8e6 schema:name doi
    99 schema:value 10.1038/nature12971
    100 rdf:type schema:PropertyValue
    101 N726479d2d0c846768271801d7618a794 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name Phosphorylation
    103 rdf:type schema:DefinedTerm
    104 N84b2ce8f766347beb4bc5162957b526c schema:name readcube_id
    105 schema:value f9a57e24eece9d743a5c566cf854ee434d6c2c4e4f8441e283817c9a2d8d62e9
    106 rdf:type schema:PropertyValue
    107 N8926e7d52ebb4fcea1f424dc8beb1946 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    108 schema:name Gene Silencing
    109 rdf:type schema:DefinedTerm
    110 N8fca0d7cde5e467e926eacf326c29bc2 schema:name pubmed_id
    111 schema:value 24531762
    112 rdf:type schema:PropertyValue
    113 N9849db88eece4b10880c0c2d187f0a1b rdf:first sg:person.0736732301.90
    114 rdf:rest N3d3b88af892144d0bde57a952b7501b6
    115 N9cd385b0c8ee473795b8c5fde75ea102 schema:volumeNumber 507
    116 rdf:type schema:PublicationVolume
    117 Na45126e9e6ef4384837b428b7c7d01e0 schema:name Clare Hall Laboratories, Cancer Research UK, London Research Institute, South Mimms EN6 3LD, UK
    118 rdf:type schema:Organization
    119 Nab2610c23cdc43df990174350c5e1042 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    120 schema:name Deoxycytidine
    121 rdf:type schema:DefinedTerm
    122 Nac1a98f3ca69492798b36bd4e0fc2034 rdf:first sg:person.01260020427.57
    123 rdf:rest Nf61655e867ea47ec939bef5af9b8d7f2
    124 Nac9c86bf37c54161b69a7f8252629174 rdf:first sg:person.01127537626.16
    125 rdf:rest N538b623d52c04fda8dff4621afd57c72
    126 Naeff6c7d31ca4507ad1b39362cd856f4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name Prokaryotic Cells
    128 rdf:type schema:DefinedTerm
    129 Naf6c516fced7426c82083317af08358b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    130 schema:name Plasmids
    131 rdf:type schema:DefinedTerm
    132 Nc38d0efcfe904d5c892021d6301f487f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    133 schema:name Base Pairing
    134 rdf:type schema:DefinedTerm
    135 Nce8c5d5adfa648989d1af749a8c414bc rdf:first sg:person.012377366202.40
    136 rdf:rest N9849db88eece4b10880c0c2d187f0a1b
    137 Ncecd3fff50904a4a8ee3b36132240418 schema:name dimensions_id
    138 schema:value pub.1052410148
    139 rdf:type schema:PropertyValue
    140 Nd4e357c520e5436fb350f627526eec8b rdf:first sg:person.01341405243.01
    141 rdf:rest Nac9c86bf37c54161b69a7f8252629174
    142 Nf0aaee7df496428bb938135a36a01b99 rdf:first sg:person.0755330446.22
    143 rdf:rest N4e21f74f77374430ad611bcf9c04a154
    144 Nf5ca5cc5efc446d5b0602d04b1527f6f schema:issueNumber 7491
    145 rdf:type schema:PublicationIssue
    146 Nf61655e867ea47ec939bef5af9b8d7f2 rdf:first sg:person.0747346652.58
    147 rdf:rest N334c2b495f164f7198a57fe6fe467339
    148 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    149 schema:name Biological Sciences
    150 rdf:type schema:DefinedTerm
    151 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    152 schema:name Genetics
    153 rdf:type schema:DefinedTerm
    154 sg:grant.2438793 http://pending.schema.org/fundedItem sg:pub.10.1038/nature12971
    155 rdf:type schema:MonetaryGrant
    156 sg:grant.2521976 http://pending.schema.org/fundedItem sg:pub.10.1038/nature12971
    157 rdf:type schema:MonetaryGrant
    158 sg:grant.4698598 http://pending.schema.org/fundedItem sg:pub.10.1038/nature12971
    159 rdf:type schema:MonetaryGrant
    160 sg:grant.4699840 http://pending.schema.org/fundedItem sg:pub.10.1038/nature12971
    161 rdf:type schema:MonetaryGrant
    162 sg:journal.1018957 schema:issn 0090-0028
    163 1476-4687
    164 schema:name Nature
    165 rdf:type schema:Periodical
    166 sg:person.01127537626.16 schema:affiliation https://www.grid.ac/institutes/grid.418856.6
    167 schema:familyName Wang
    168 schema:givenName Yanli
    169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01127537626.16
    170 rdf:type schema:Person
    171 sg:person.01135023106.43 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    172 schema:familyName Janssen
    173 schema:givenName Jorijn H.
    174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01135023106.43
    175 rdf:type schema:Person
    176 sg:person.012377366202.40 schema:affiliation https://www.grid.ac/institutes/grid.5515.4
    177 schema:familyName Berenguer
    178 schema:givenName José
    179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012377366202.40
    180 rdf:type schema:Person
    181 sg:person.01260020427.57 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    182 schema:familyName Swarts
    183 schema:givenName Daan C.
    184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01260020427.57
    185 rdf:type schema:Person
    186 sg:person.01321677476.16 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    187 schema:familyName van der Oost
    188 schema:givenName John
    189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321677476.16
    190 rdf:type schema:Person
    191 sg:person.01341405243.01 schema:affiliation Na45126e9e6ef4384837b428b7c7d01e0
    192 schema:familyName Snijders
    193 schema:givenName Ambrosius P.
    194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01341405243.01
    195 rdf:type schema:Person
    196 sg:person.016225305307.50 schema:affiliation https://www.grid.ac/institutes/grid.51462.34
    197 schema:familyName Patel
    198 schema:givenName Dinshaw J.
    199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016225305307.50
    200 rdf:type schema:Person
    201 sg:person.0710321253.01 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    202 schema:familyName Westra
    203 schema:givenName Edze R.
    204 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0710321253.01
    205 rdf:type schema:Person
    206 sg:person.0736732301.90 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    207 schema:familyName Brouns
    208 schema:givenName Stan J. J.
    209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0736732301.90
    210 rdf:type schema:Person
    211 sg:person.0747346652.58 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    212 schema:familyName Jore
    213 schema:givenName Matthijs M.
    214 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0747346652.58
    215 rdf:type schema:Person
    216 sg:person.0755330446.22 schema:affiliation https://www.grid.ac/institutes/grid.4818.5
    217 schema:familyName Zhu
    218 schema:givenName Yifan
    219 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0755330446.22
    220 rdf:type schema:Person
    221 sg:pub.10.1007/978-1-4419-7823-3_1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041025541
    222 https://doi.org/10.1007/978-1-4419-7823-3_1
    223 rdf:type schema:CreativeWork
    224 sg:pub.10.1038/nature03514 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021329082
    225 https://doi.org/10.1038/nature03514
    226 rdf:type schema:CreativeWork
    227 sg:pub.10.1038/nature07315 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020271916
    228 https://doi.org/10.1038/nature07315
    229 rdf:type schema:CreativeWork
    230 sg:pub.10.1038/nature07666 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029382170
    231 https://doi.org/10.1038/nature07666
    232 rdf:type schema:CreativeWork
    233 sg:pub.10.1038/nature08434 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042379011
    234 https://doi.org/10.1038/nature08434
    235 rdf:type schema:CreativeWork
    236 sg:pub.10.1038/nature09039 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022404163
    237 https://doi.org/10.1038/nature09039
    238 rdf:type schema:CreativeWork
    239 sg:pub.10.1038/nature11211 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037387037
    240 https://doi.org/10.1038/nature11211
    241 rdf:type schema:CreativeWork
    242 sg:pub.10.1038/nrmicro1088 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002249031
    243 https://doi.org/10.1038/nrmicro1088
    244 rdf:type schema:CreativeWork
    245 sg:pub.10.1186/1745-6150-4-29 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015797830
    246 https://doi.org/10.1186/1745-6150-4-29
    247 rdf:type schema:CreativeWork
    248 https://doi.org/10.1016/j.molcel.2005.07.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015155076
    249 rdf:type schema:CreativeWork
    250 https://doi.org/10.1016/j.molcel.2012.03.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024792403
    251 rdf:type schema:CreativeWork
    252 https://doi.org/10.1016/j.molcel.2013.08.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041420574
    253 rdf:type schema:CreativeWork
    254 https://doi.org/10.1016/s0168-9525(02)02690-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011065957
    255 rdf:type schema:CreativeWork
    256 https://doi.org/10.1038/emboj.2012.204 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004918093
    257 rdf:type schema:CreativeWork
    258 https://doi.org/10.1038/sj.emboj.7601094 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029981632
    259 rdf:type schema:CreativeWork
    260 https://doi.org/10.1073/pnas.1321032111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019838218
    261 rdf:type schema:CreativeWork
    262 https://doi.org/10.1093/bib/bbs012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022817326
    263 rdf:type schema:CreativeWork
    264 https://doi.org/10.1093/bioinformatics/btp352 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023014918
    265 rdf:type schema:CreativeWork
    266 https://doi.org/10.1093/emboj/20.23.6877 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004775429
    267 rdf:type schema:CreativeWork
    268 https://doi.org/10.1093/nar/21.3.463 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016360203
    269 rdf:type schema:CreativeWork
    270 https://doi.org/10.1101/cshperspect.a003772 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010792735
    271 rdf:type schema:CreativeWork
    272 https://doi.org/10.1111/j.1365-2958.2007.05687.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1005339956
    273 rdf:type schema:CreativeWork
    274 https://doi.org/10.1111/j.1462-2920.2007.01482.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1051181196
    275 rdf:type schema:CreativeWork
    276 https://doi.org/10.1111/j.1574-6968.1988.tb13940.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1040837810
    277 rdf:type schema:CreativeWork
    278 https://doi.org/10.1111/j.1574-6968.2008.01389.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1035594214
    279 rdf:type schema:CreativeWork
    280 https://doi.org/10.1111/j.1574-6976.2008.00160.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1017595065
    281 rdf:type schema:CreativeWork
    282 https://doi.org/10.1126/science.1102513 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018006760
    283 rdf:type schema:CreativeWork
    284 https://doi.org/10.1128/jb.166.1.338-340.1986 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062714841
    285 rdf:type schema:CreativeWork
    286 https://doi.org/10.1128/jb.176.5.1251-1259.1994 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062723464
    287 rdf:type schema:CreativeWork
    288 https://doi.org/10.1371/journal.pone.0020425 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035941706
    289 rdf:type schema:CreativeWork
    290 https://www.grid.ac/institutes/grid.418856.6 schema:alternateName Institute of Biophysics
    291 schema:name Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    292 rdf:type schema:Organization
    293 https://www.grid.ac/institutes/grid.4818.5 schema:alternateName Wageningen University & Research
    294 schema:name Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, the Netherlands
    295 rdf:type schema:Organization
    296 https://www.grid.ac/institutes/grid.51462.34 schema:alternateName Memorial Sloan Kettering Cancer Center
    297 schema:name Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10065, USA
    298 rdf:type schema:Organization
    299 https://www.grid.ac/institutes/grid.5515.4 schema:alternateName Autonomous University of Madrid
    300 schema:name Centro de Biología Molecular Severo Ochoa, UAM-CSIC, Campus de Cantoblanco, 28049 Madrid, Spain
    301 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...