Biochemical reconstitution of topological DNA binding by the cohesin ring View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2014-01

AUTHORS

Yasuto Murayama, Frank Uhlmann

ABSTRACT

Cohesion between sister chromatids, mediated by the chromosomal cohesin complex, is a prerequisite for faithful chromosome segregation in mitosis. Cohesin also has vital roles in DNA repair and transcriptional regulation. The ring-shaped cohesin complex is thought to encircle sister DNA strands, but its molecular mechanism of action is poorly understood and the biochemical reconstitution of cohesin activity in vitro has remained an unattained goal. Here we reconstitute cohesin loading onto DNA using purified fission yeast cohesin and its loader complex, Mis4(Scc2)-Ssl3(Scc4) (Schizosaccharomyces pombe gene names appear throughout with their more commonly known Saccharomyces cerevisiae counterparts added in superscript). Incubation of cohesin with DNA leads to spontaneous topological loading, but this remains inefficient. The loader contacts cohesin at multiple sites around the ring circumference, including the hitherto enigmatic Psc3(Scc3) subunit, and stimulates cohesin's ATPase, resulting in efficient topological loading. The in vitro reconstitution of cohesin loading onto DNA provides mechanistic insight into the initial steps of the establishment of sister chromatid cohesion and other chromosomal processes mediated by cohesin. More... »

PAGES

367

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nature12867

DOI

http://dx.doi.org/10.1038/nature12867

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1052123326

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24291789


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adenosine Triphosphatases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Cycle Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromatids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromosomal Proteins, Non-Histone", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA-Binding Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nucleic Acid Conformation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Binding", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Schizosaccharomyces", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Schizosaccharomyces pombe Proteins", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "The Francis Crick Institute", 
          "id": "https://www.grid.ac/institutes/grid.451388.3", 
          "name": [
            "Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln\u2019s Inn Fields, London WC2A 3LY, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Murayama", 
        "givenName": "Yasuto", 
        "id": "sg:person.0741275461.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0741275461.48"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Francis Crick Institute", 
          "id": "https://www.grid.ac/institutes/grid.451388.3", 
          "name": [
            "Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln\u2019s Inn Fields, London WC2A 3LY, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Uhlmann", 
        "givenName": "Frank", 
        "id": "sg:person.01000126743.46", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01000126743.46"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/s1097-2765(02)00515-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000676117"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)00130-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000699198"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cub.2003.10.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006728348"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.13.3.320", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007713448"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cub.2006.03.037", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007718724"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(01)80008-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010098308"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1097-2765(00)80420-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010218478"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1203619", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011556378"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/cdg222", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013755659"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.12.13.1986", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013758723"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1157774", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015905780"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/gb-2009-10-5-r52", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016806100", 
          "https://doi.org/10.1186/gb-2009-10-5-r52"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0926-6585(66)90333-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017520634"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0926-6585(66)90333-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017520634"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.832000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018652780"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2009.02.035", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020552122"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1206840109", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021847859"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.200111002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023988462"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0076-6879(91)94059-l", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024347016"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng1779", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025561634", 
          "https://doi.org/10.1038/ng1779"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ng1779", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025561634", 
          "https://doi.org/10.1038/ng1779"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsmb.2087", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026469715", 
          "https://doi.org/10.1038/nsmb.2087"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbrc.2008.07.015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027833810"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(01)00066-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031066418"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(88)90185-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031424271"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(88)90185-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031424271"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/20.24.6605", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032075569"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(01)80007-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033103858"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/cdg247", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033614010"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2007.04.018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033911906"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1097-2765(00)80398-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034891318"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.150.3.405", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035641884"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature02742", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039278013", 
          "https://doi.org/10.1038/nature02742"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature02742", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039278013", 
          "https://doi.org/10.1038/nature02742"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsmb.2038", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039725960", 
          "https://doi.org/10.1038/nsmb.2038"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature07098", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040029942", 
          "https://doi.org/10.1038/nature07098"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.1683308", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042983580"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cub.2003.10.036", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045384304"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2011.02.038", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045713243"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature06634", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045728553", 
          "https://doi.org/10.1038/nature06634"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb2349", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047221444", 
          "https://doi.org/10.1038/ncb2349"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.151.4.749", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047618205"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.12.21.3408", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048291444"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.2037811", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050317466"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(01)00271-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051819979"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1107504108", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053712229"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.1675708", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060407269"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1157880", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062457727"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1096/fasebj.14.3.581", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074598871"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081754819", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2014-01", 
    "datePublishedReg": "2014-01-01", 
    "description": "Cohesion between sister chromatids, mediated by the chromosomal cohesin complex, is a prerequisite for faithful chromosome segregation in mitosis. Cohesin also has vital roles in DNA repair and transcriptional regulation. The ring-shaped cohesin complex is thought to encircle sister DNA strands, but its molecular mechanism of action is poorly understood and the biochemical reconstitution of cohesin activity in vitro has remained an unattained goal. Here we reconstitute cohesin loading onto DNA using purified fission yeast cohesin and its loader complex, Mis4(Scc2)-Ssl3(Scc4) (Schizosaccharomyces pombe gene names appear throughout with their more commonly known Saccharomyces cerevisiae counterparts added in superscript). Incubation of cohesin with DNA leads to spontaneous topological loading, but this remains inefficient. The loader contacts cohesin at multiple sites around the ring circumference, including the hitherto enigmatic Psc3(Scc3) subunit, and stimulates cohesin's ATPase, resulting in efficient topological loading. The in vitro reconstitution of cohesin loading onto DNA provides mechanistic insight into the initial steps of the establishment of sister chromatid cohesion and other chromosomal processes mediated by cohesin. ", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nature12867", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.5149568", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7483", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "505"
      }
    ], 
    "name": "Biochemical reconstitution of topological DNA binding by the cohesin ring", 
    "pagination": "367", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "f8480ba1163e6b686c6b83d950f9ed303697afc8ce5d6047241486e876a873b7"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "24291789"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nature12867"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1052123326"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nature12867", 
      "https://app.dimensions.ai/details/publication/pub.1052123326"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T12:59", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8659_00000426.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/nature12867"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature12867'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature12867'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature12867'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature12867'


 

This table displays all metadata directly associated to this object as RDF triples.

263 TRIPLES      21 PREDICATES      85 URIs      31 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nature12867 schema:about N3b1052daa71f4fcabac63e6418e05da8
2 N52245fa65e1349a3b4f512249bca82e4
3 N608173c394d64b1ab09e6bf906a281d5
4 N65f96d98a15a42649a9af2bf9056c143
5 N8235c04af0e84f7b9f5f0d5c939840a7
6 N93742878aef940869fc8eebc868487cc
7 N966ce1753dd24ddc94febc0bd42d06f3
8 Nb9e5f0f4c8194b7287d255b09738dade
9 Nc4d8fb9564e7495b83767a1ad8c6a292
10 Ncd6a76f983094a04a7987ae1a70269c8
11 anzsrc-for:06
12 anzsrc-for:0604
13 schema:author N3d4d396fe46f4fa79e0a6ede2b3caba1
14 schema:citation sg:pub.10.1038/nature02742
15 sg:pub.10.1038/nature06634
16 sg:pub.10.1038/nature07098
17 sg:pub.10.1038/ncb2349
18 sg:pub.10.1038/ng1779
19 sg:pub.10.1038/nsmb.2038
20 sg:pub.10.1038/nsmb.2087
21 sg:pub.10.1186/gb-2009-10-5-r52
22 https://app.dimensions.ai/details/publication/pub.1081754819
23 https://doi.org/10.1016/0076-6879(91)94059-l
24 https://doi.org/10.1016/0378-1119(88)90185-0
25 https://doi.org/10.1016/0926-6585(66)90333-5
26 https://doi.org/10.1016/j.bbrc.2008.07.015
27 https://doi.org/10.1016/j.cell.2009.02.035
28 https://doi.org/10.1016/j.cell.2011.02.038
29 https://doi.org/10.1016/j.cub.2003.10.030
30 https://doi.org/10.1016/j.cub.2003.10.036
31 https://doi.org/10.1016/j.cub.2006.03.037
32 https://doi.org/10.1016/j.molcel.2007.04.018
33 https://doi.org/10.1016/s0092-8674(00)00130-6
34 https://doi.org/10.1016/s0092-8674(01)80007-6
35 https://doi.org/10.1016/s0092-8674(01)80008-8
36 https://doi.org/10.1016/s0960-9822(01)00066-5
37 https://doi.org/10.1016/s0960-9822(01)00271-8
38 https://doi.org/10.1016/s1097-2765(00)80398-6
39 https://doi.org/10.1016/s1097-2765(00)80420-7
40 https://doi.org/10.1016/s1097-2765(02)00515-4
41 https://doi.org/10.1073/pnas.1107504108
42 https://doi.org/10.1073/pnas.1206840109
43 https://doi.org/10.1083/jcb.150.3.405
44 https://doi.org/10.1083/jcb.151.4.749
45 https://doi.org/10.1083/jcb.200111002
46 https://doi.org/10.1093/emboj/cdg222
47 https://doi.org/10.1093/emboj/cdg247
48 https://doi.org/10.1093/nar/20.24.6605
49 https://doi.org/10.1096/fasebj.14.3.581
50 https://doi.org/10.1101/gad.12.13.1986
51 https://doi.org/10.1101/gad.12.21.3408
52 https://doi.org/10.1101/gad.13.3.320
53 https://doi.org/10.1101/gad.1675708
54 https://doi.org/10.1101/gad.1683308
55 https://doi.org/10.1101/gad.2037811
56 https://doi.org/10.1101/gad.832000
57 https://doi.org/10.1126/science.1157774
58 https://doi.org/10.1126/science.1157880
59 https://doi.org/10.1126/science.1203619
60 schema:datePublished 2014-01
61 schema:datePublishedReg 2014-01-01
62 schema:description Cohesion between sister chromatids, mediated by the chromosomal cohesin complex, is a prerequisite for faithful chromosome segregation in mitosis. Cohesin also has vital roles in DNA repair and transcriptional regulation. The ring-shaped cohesin complex is thought to encircle sister DNA strands, but its molecular mechanism of action is poorly understood and the biochemical reconstitution of cohesin activity in vitro has remained an unattained goal. Here we reconstitute cohesin loading onto DNA using purified fission yeast cohesin and its loader complex, Mis4(Scc2)-Ssl3(Scc4) (Schizosaccharomyces pombe gene names appear throughout with their more commonly known Saccharomyces cerevisiae counterparts added in superscript). Incubation of cohesin with DNA leads to spontaneous topological loading, but this remains inefficient. The loader contacts cohesin at multiple sites around the ring circumference, including the hitherto enigmatic Psc3(Scc3) subunit, and stimulates cohesin's ATPase, resulting in efficient topological loading. The in vitro reconstitution of cohesin loading onto DNA provides mechanistic insight into the initial steps of the establishment of sister chromatid cohesion and other chromosomal processes mediated by cohesin.
63 schema:genre research_article
64 schema:inLanguage en
65 schema:isAccessibleForFree true
66 schema:isPartOf Na622ee4bb9d044b78a77bd481ceed7d3
67 Nedfa09e9d9774e6ca8fcad3d43d73284
68 sg:journal.1018957
69 schema:name Biochemical reconstitution of topological DNA binding by the cohesin ring
70 schema:pagination 367
71 schema:productId N59b2b923166e4848a215b4c4c4f2cb77
72 N7e55f60dcd4e42b29b41590de8810b9c
73 N9dc5b2999e014867952daf4a909c0c61
74 Nb5e20a72db834e259c06875156c134a4
75 Ncc162e394a5b45218caf5a1ea89e5038
76 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052123326
77 https://doi.org/10.1038/nature12867
78 schema:sdDatePublished 2019-04-10T12:59
79 schema:sdLicense https://scigraph.springernature.com/explorer/license/
80 schema:sdPublisher N1e896e2cb5474075adbbd7cfb0193e1f
81 schema:url https://www.nature.com/articles/nature12867
82 sgo:license sg:explorer/license/
83 sgo:sdDataset articles
84 rdf:type schema:ScholarlyArticle
85 N1e896e2cb5474075adbbd7cfb0193e1f schema:name Springer Nature - SN SciGraph project
86 rdf:type schema:Organization
87 N3b1052daa71f4fcabac63e6418e05da8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Protein Binding
89 rdf:type schema:DefinedTerm
90 N3d4d396fe46f4fa79e0a6ede2b3caba1 rdf:first sg:person.0741275461.48
91 rdf:rest N9e51f6abbfb74f3f9a7a035b332141c3
92 N52245fa65e1349a3b4f512249bca82e4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name DNA
94 rdf:type schema:DefinedTerm
95 N59b2b923166e4848a215b4c4c4f2cb77 schema:name doi
96 schema:value 10.1038/nature12867
97 rdf:type schema:PropertyValue
98 N608173c394d64b1ab09e6bf906a281d5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name DNA-Binding Proteins
100 rdf:type schema:DefinedTerm
101 N65f96d98a15a42649a9af2bf9056c143 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Schizosaccharomyces
103 rdf:type schema:DefinedTerm
104 N7e55f60dcd4e42b29b41590de8810b9c schema:name readcube_id
105 schema:value f8480ba1163e6b686c6b83d950f9ed303697afc8ce5d6047241486e876a873b7
106 rdf:type schema:PropertyValue
107 N8235c04af0e84f7b9f5f0d5c939840a7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Chromatids
109 rdf:type schema:DefinedTerm
110 N93742878aef940869fc8eebc868487cc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Chromosomal Proteins, Non-Histone
112 rdf:type schema:DefinedTerm
113 N966ce1753dd24ddc94febc0bd42d06f3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Nucleic Acid Conformation
115 rdf:type schema:DefinedTerm
116 N9dc5b2999e014867952daf4a909c0c61 schema:name dimensions_id
117 schema:value pub.1052123326
118 rdf:type schema:PropertyValue
119 N9e51f6abbfb74f3f9a7a035b332141c3 rdf:first sg:person.01000126743.46
120 rdf:rest rdf:nil
121 Na622ee4bb9d044b78a77bd481ceed7d3 schema:issueNumber 7483
122 rdf:type schema:PublicationIssue
123 Nb5e20a72db834e259c06875156c134a4 schema:name nlm_unique_id
124 schema:value 0410462
125 rdf:type schema:PropertyValue
126 Nb9e5f0f4c8194b7287d255b09738dade schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Schizosaccharomyces pombe Proteins
128 rdf:type schema:DefinedTerm
129 Nc4d8fb9564e7495b83767a1ad8c6a292 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Adenosine Triphosphatases
131 rdf:type schema:DefinedTerm
132 Ncc162e394a5b45218caf5a1ea89e5038 schema:name pubmed_id
133 schema:value 24291789
134 rdf:type schema:PropertyValue
135 Ncd6a76f983094a04a7987ae1a70269c8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Cell Cycle Proteins
137 rdf:type schema:DefinedTerm
138 Nedfa09e9d9774e6ca8fcad3d43d73284 schema:volumeNumber 505
139 rdf:type schema:PublicationVolume
140 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
141 schema:name Biological Sciences
142 rdf:type schema:DefinedTerm
143 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
144 schema:name Genetics
145 rdf:type schema:DefinedTerm
146 sg:grant.5149568 http://pending.schema.org/fundedItem sg:pub.10.1038/nature12867
147 rdf:type schema:MonetaryGrant
148 sg:journal.1018957 schema:issn 0090-0028
149 1476-4687
150 schema:name Nature
151 rdf:type schema:Periodical
152 sg:person.01000126743.46 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
153 schema:familyName Uhlmann
154 schema:givenName Frank
155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01000126743.46
156 rdf:type schema:Person
157 sg:person.0741275461.48 schema:affiliation https://www.grid.ac/institutes/grid.451388.3
158 schema:familyName Murayama
159 schema:givenName Yasuto
160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0741275461.48
161 rdf:type schema:Person
162 sg:pub.10.1038/nature02742 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039278013
163 https://doi.org/10.1038/nature02742
164 rdf:type schema:CreativeWork
165 sg:pub.10.1038/nature06634 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045728553
166 https://doi.org/10.1038/nature06634
167 rdf:type schema:CreativeWork
168 sg:pub.10.1038/nature07098 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040029942
169 https://doi.org/10.1038/nature07098
170 rdf:type schema:CreativeWork
171 sg:pub.10.1038/ncb2349 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047221444
172 https://doi.org/10.1038/ncb2349
173 rdf:type schema:CreativeWork
174 sg:pub.10.1038/ng1779 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025561634
175 https://doi.org/10.1038/ng1779
176 rdf:type schema:CreativeWork
177 sg:pub.10.1038/nsmb.2038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039725960
178 https://doi.org/10.1038/nsmb.2038
179 rdf:type schema:CreativeWork
180 sg:pub.10.1038/nsmb.2087 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026469715
181 https://doi.org/10.1038/nsmb.2087
182 rdf:type schema:CreativeWork
183 sg:pub.10.1186/gb-2009-10-5-r52 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016806100
184 https://doi.org/10.1186/gb-2009-10-5-r52
185 rdf:type schema:CreativeWork
186 https://app.dimensions.ai/details/publication/pub.1081754819 schema:CreativeWork
187 https://doi.org/10.1016/0076-6879(91)94059-l schema:sameAs https://app.dimensions.ai/details/publication/pub.1024347016
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1016/0378-1119(88)90185-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031424271
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1016/0926-6585(66)90333-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017520634
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1016/j.bbrc.2008.07.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027833810
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1016/j.cell.2009.02.035 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020552122
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1016/j.cell.2011.02.038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045713243
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1016/j.cub.2003.10.030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006728348
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1016/j.cub.2003.10.036 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045384304
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1016/j.cub.2006.03.037 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007718724
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1016/j.molcel.2007.04.018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033911906
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1016/s0092-8674(00)00130-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000699198
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1016/s0092-8674(01)80007-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033103858
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1016/s0092-8674(01)80008-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010098308
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1016/s0960-9822(01)00066-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031066418
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1016/s0960-9822(01)00271-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051819979
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1016/s1097-2765(00)80398-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034891318
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1016/s1097-2765(00)80420-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010218478
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1016/s1097-2765(02)00515-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000676117
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1073/pnas.1107504108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053712229
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1073/pnas.1206840109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021847859
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1083/jcb.150.3.405 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035641884
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1083/jcb.151.4.749 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047618205
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1083/jcb.200111002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023988462
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1093/emboj/cdg222 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013755659
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1093/emboj/cdg247 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033614010
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1093/nar/20.24.6605 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032075569
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1096/fasebj.14.3.581 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074598871
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1101/gad.12.13.1986 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013758723
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1101/gad.12.21.3408 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048291444
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1101/gad.13.3.320 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007713448
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1101/gad.1675708 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060407269
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1101/gad.1683308 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042983580
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1101/gad.2037811 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050317466
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1101/gad.832000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018652780
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1126/science.1157774 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015905780
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1126/science.1157880 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062457727
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1126/science.1203619 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011556378
260 rdf:type schema:CreativeWork
261 https://www.grid.ac/institutes/grid.451388.3 schema:alternateName The Francis Crick Institute
262 schema:name Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, 44 Lincoln’s Inn Fields, London WC2A 3LY, UK
263 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...