Primary cilia are specialized calcium signalling organelles View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2013-12

AUTHORS

Markus Delling, Paul G. DeCaen, Julia F. Doerner, Sebastien Febvay, David E. Clapham

ABSTRACT

Primary cilia are solitary, non-motile extensions of the centriole found on nearly all nucleated eukaryotic cells between cell divisions. Only ∼200-300 nm in diameter and a few micrometres long, they are separated from the cytoplasm by the ciliary neck and basal body. Often called sensory cilia, they are thought to receive chemical and mechanical stimuli and initiate specific cellular signal transduction pathways. When activated by a ligand, hedgehog pathway proteins, such as GLI2 and smoothened (SMO), translocate from the cell into the cilium. Mutations in primary ciliary proteins are associated with severe developmental defects. The ionic conditions, permeability of the primary cilia membrane, and effectiveness of the diffusion barriers between the cilia and cell body are unknown. Here we show that cilia are a unique calcium compartment regulated by a heteromeric TRP channel, PKD1L1-PKD2L1, in mice and humans. In contrast to the hypothesis that polycystin (PKD) channels initiate changes in ciliary calcium that are conducted into the cytoplasm, we show that changes in ciliary calcium concentration occur without substantially altering global cytoplasmic calcium. PKD1L1-PKD2L1 acts as a ciliary calcium channel controlling ciliary calcium concentration and thereby modifying SMO-activated GLI2 translocation and GLI1 expression. More... »

PAGES

311

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nature12833

DOI

http://dx.doi.org/10.1038/nature12833

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1023302129

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24336288


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Calcium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Calcium Channels", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Calcium Signaling", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cilia", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytoplasm", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hedgehog Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kruppel-Like Transcription Factors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Membrane Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nuclear Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Organelles", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Receptors, Cell Surface", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Receptors, G-Protein-Coupled", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Smoothened Receptor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Zinc Finger Protein GLI1", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Zinc Finger Protein Gli2", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Boston Children's Hospital", 
          "id": "https://www.grid.ac/institutes/grid.2515.3", 
          "name": [
            "Howard Hughes Medical Institute, Department of Cardiology, Boston Children\u2019s Hospital, 320 Longwood Avenue, Boston, Massachusetts 02115, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Delling", 
        "givenName": "Markus", 
        "id": "sg:person.01012045614.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01012045614.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Boston Children's Hospital", 
          "id": "https://www.grid.ac/institutes/grid.2515.3", 
          "name": [
            "Howard Hughes Medical Institute, Department of Cardiology, Boston Children\u2019s Hospital, 320 Longwood Avenue, Boston, Massachusetts 02115, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "DeCaen", 
        "givenName": "Paul G.", 
        "id": "sg:person.01060161014.72", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01060161014.72"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Boston Children's Hospital", 
          "id": "https://www.grid.ac/institutes/grid.2515.3", 
          "name": [
            "Howard Hughes Medical Institute, Department of Cardiology, Boston Children\u2019s Hospital, 320 Longwood Avenue, Boston, Massachusetts 02115, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Doerner", 
        "givenName": "Julia F.", 
        "id": "sg:person.0725133462.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0725133462.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Boston Children's Hospital", 
          "id": "https://www.grid.ac/institutes/grid.2515.3", 
          "name": [
            "Howard Hughes Medical Institute, Department of Cardiology, Boston Children\u2019s Hospital, 320 Longwood Avenue, Boston, Massachusetts 02115, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Febvay", 
        "givenName": "Sebastien", 
        "id": "sg:person.01024301701.91", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01024301701.91"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Harvard University", 
          "id": "https://www.grid.ac/institutes/grid.38142.3c", 
          "name": [
            "Howard Hughes Medical Institute, Department of Cardiology, Boston Children\u2019s Hospital, 320 Longwood Avenue, Boston, Massachusetts 02115, USA", 
            "Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Clapham", 
        "givenName": "David E.", 
        "id": "sg:person.0672201126.59", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0672201126.59"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1073/pnas.0912180106", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002649166"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/stke.2192004pl5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008052869"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nn.2928", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009160296", 
          "https://doi.org/10.1038/nn.2928"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature12832", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010806074", 
          "https://doi.org/10.1038/nature12832"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08895", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016398543", 
          "https://doi.org/10.1038/nature08895"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature08895", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016398543", 
          "https://doi.org/10.1038/nature08895"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0505328102", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023197118"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00383-010-2622-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023230919", 
          "https://doi.org/10.1007/s00383-010-2622-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00383-010-2622-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023230919", 
          "https://doi.org/10.1007/s00383-010-2622-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0020007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024505509"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.devcel.2012.04.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030047319"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.1902910", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031351817"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1398", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035553144", 
          "https://doi.org/10.1038/nmeth.1398"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth.1398", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035553144", 
          "https://doi.org/10.1038/nmeth.1398"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/emboj.2011.110", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037015123"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.201004108", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037335995"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00232-001-0075-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037575656", 
          "https://doi.org/10.1007/s00232-001-0075-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2007.11.028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041047694"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nn1698", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046380817", 
          "https://doi.org/10.1038/nn1698"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nn1698", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046380817", 
          "https://doi.org/10.1038/nn1698"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4615-0067-4_44", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047319687", 
          "https://doi.org/10.1007/978-1-4615-0067-4_44"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4615-0067-4_44", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047319687", 
          "https://doi.org/10.1007/978-1-4615-0067-4_44"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/bioinformatics/btp184", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047735637"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrg2774", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047735807", 
          "https://doi.org/10.1038/nrg2774"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrg2774", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047735807", 
          "https://doi.org/10.1038/nrg2774"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04117", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048565750", 
          "https://doi.org/10.1038/nature04117"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04117", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048565750", 
          "https://doi.org/10.1038/nature04117"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04117", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048565750", 
          "https://doi.org/10.1038/nature04117"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cub.2013.02.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048566772"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1208592", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049468469"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/0300985809353553", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051976376"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/0300985809353553", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051976376"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/dev.058149", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053246160"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074643455", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2013-12", 
    "datePublishedReg": "2013-12-01", 
    "description": "Primary cilia are solitary, non-motile extensions of the centriole found on nearly all nucleated eukaryotic cells between cell divisions. Only \u223c200-300\u2009nm in diameter and a few micrometres long, they are separated from the cytoplasm by the ciliary neck and basal body. Often called sensory cilia, they are thought to receive chemical and mechanical stimuli and initiate specific cellular signal transduction pathways. When activated by a ligand, hedgehog pathway proteins, such as GLI2 and smoothened (SMO), translocate from the cell into the cilium. Mutations in primary ciliary proteins are associated with severe developmental defects. The ionic conditions, permeability of the primary cilia membrane, and effectiveness of the diffusion barriers between the cilia and cell body are unknown. Here we show that cilia are a unique calcium compartment regulated by a heteromeric TRP channel, PKD1L1-PKD2L1, in mice and humans. In contrast to the hypothesis that polycystin (PKD) channels initiate changes in ciliary calcium that are conducted into the cytoplasm, we show that changes in ciliary calcium concentration occur without substantially altering global cytoplasmic calcium. PKD1L1-PKD2L1 acts as a ciliary calcium channel controlling ciliary calcium concentration and thereby modifying SMO-activated GLI2 translocation and GLI1 expression. ", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nature12833", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2684748", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2437483", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2439252", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7479", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "504"
      }
    ], 
    "name": "Primary cilia are specialized calcium signalling organelles", 
    "pagination": "311", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "6f0464825814db0a71e509c92312ed58a7979584967fb014dbf5b4f36734417f"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "24336288"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nature12833"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1023302129"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nature12833", 
      "https://app.dimensions.ai/details/publication/pub.1023302129"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T14:47", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8663_00000424.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/nature12833"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature12833'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature12833'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature12833'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature12833'


 

This table displays all metadata directly associated to this object as RDF triples.

275 TRIPLES      21 PREDICATES      75 URIs      42 LITERALS      30 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nature12833 schema:about N198d0aa3bbb5463ab30a496354b2e187
2 N299aa623680d4b53a380dc39e23e56fd
3 N318486309ec1400aae94b9725f1bd785
4 N4257df4c1c704557a1953435fa41383b
5 N5039f20cab874e759ed7a06ef9e50426
6 N6f17a8618f0e42e9a845038881a0dd79
7 N7cf237dedc8b45988e66f34424d34368
8 N8c724a0677144ab4a8e55f5b2532e8b2
9 N8fdfd0c322494472a8e9c76126c83ff8
10 N9602ccf5181247ffae00a8ca8f8ed80b
11 N9bf542b177864166ad3587855bba445f
12 Na2846660669247fd99a1ab6500c1bec2
13 Nb4062dbd35b14721905baf5eeaa201b8
14 Nb6e413d58f7a442191170d814ac746c8
15 Ncc5ab08c0def4ba7869044036e6eff16
16 Nd89e3568f34e41818c3b3183d349a726
17 Ndd397bf18fb7465288416c8caa003ef3
18 Ne7d813eed97e467c9ca549d4a6892a46
19 Neaf0928bc66d48ce93c44040021e9fc0
20 Nf1fc3df24aca4d7d8b36ce852ca9f190
21 Nf2b75a20f1064bf480e580bff92e898a
22 anzsrc-for:06
23 anzsrc-for:0601
24 schema:author N9d907d74912d467eaad1a01a82a97629
25 schema:citation sg:pub.10.1007/978-1-4615-0067-4_44
26 sg:pub.10.1007/s00232-001-0075-4
27 sg:pub.10.1007/s00383-010-2622-5
28 sg:pub.10.1038/nature04117
29 sg:pub.10.1038/nature08895
30 sg:pub.10.1038/nature12832
31 sg:pub.10.1038/nmeth.1398
32 sg:pub.10.1038/nn.2928
33 sg:pub.10.1038/nn1698
34 sg:pub.10.1038/nrg2774
35 https://app.dimensions.ai/details/publication/pub.1074643455
36 https://doi.org/10.1016/j.cell.2007.11.028
37 https://doi.org/10.1016/j.cub.2013.02.007
38 https://doi.org/10.1016/j.devcel.2012.04.009
39 https://doi.org/10.1038/emboj.2011.110
40 https://doi.org/10.1073/pnas.0505328102
41 https://doi.org/10.1073/pnas.0912180106
42 https://doi.org/10.1083/jcb.201004108
43 https://doi.org/10.1093/bioinformatics/btp184
44 https://doi.org/10.1101/gad.1902910
45 https://doi.org/10.1126/science.1208592
46 https://doi.org/10.1126/stke.2192004pl5
47 https://doi.org/10.1177/0300985809353553
48 https://doi.org/10.1242/dev.058149
49 https://doi.org/10.1371/journal.pone.0020007
50 schema:datePublished 2013-12
51 schema:datePublishedReg 2013-12-01
52 schema:description Primary cilia are solitary, non-motile extensions of the centriole found on nearly all nucleated eukaryotic cells between cell divisions. Only ∼200-300 nm in diameter and a few micrometres long, they are separated from the cytoplasm by the ciliary neck and basal body. Often called sensory cilia, they are thought to receive chemical and mechanical stimuli and initiate specific cellular signal transduction pathways. When activated by a ligand, hedgehog pathway proteins, such as GLI2 and smoothened (SMO), translocate from the cell into the cilium. Mutations in primary ciliary proteins are associated with severe developmental defects. The ionic conditions, permeability of the primary cilia membrane, and effectiveness of the diffusion barriers between the cilia and cell body are unknown. Here we show that cilia are a unique calcium compartment regulated by a heteromeric TRP channel, PKD1L1-PKD2L1, in mice and humans. In contrast to the hypothesis that polycystin (PKD) channels initiate changes in ciliary calcium that are conducted into the cytoplasm, we show that changes in ciliary calcium concentration occur without substantially altering global cytoplasmic calcium. PKD1L1-PKD2L1 acts as a ciliary calcium channel controlling ciliary calcium concentration and thereby modifying SMO-activated GLI2 translocation and GLI1 expression.
53 schema:genre research_article
54 schema:inLanguage en
55 schema:isAccessibleForFree true
56 schema:isPartOf N821b7ea275f64f529c162d78daf97030
57 Nbc22feafc8f14bdf9bbfa4298aa5b4eb
58 sg:journal.1018957
59 schema:name Primary cilia are specialized calcium signalling organelles
60 schema:pagination 311
61 schema:productId N2432d9093f0c4d1693cc343a52e423b9
62 N5875219efaa74079a4a8f85c49936621
63 N5b275b91c33941a290d835c9e2c71476
64 Na25437586e0243c0bb193db8229a7276
65 Nb6071e1cc54642f3944e53a99907de6f
66 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023302129
67 https://doi.org/10.1038/nature12833
68 schema:sdDatePublished 2019-04-10T14:47
69 schema:sdLicense https://scigraph.springernature.com/explorer/license/
70 schema:sdPublisher N1c7223ed32454b388352afa17e83a241
71 schema:url https://www.nature.com/articles/nature12833
72 sgo:license sg:explorer/license/
73 sgo:sdDataset articles
74 rdf:type schema:ScholarlyArticle
75 N198d0aa3bbb5463ab30a496354b2e187 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
76 schema:name Humans
77 rdf:type schema:DefinedTerm
78 N1c7223ed32454b388352afa17e83a241 schema:name Springer Nature - SN SciGraph project
79 rdf:type schema:Organization
80 N2432d9093f0c4d1693cc343a52e423b9 schema:name nlm_unique_id
81 schema:value 0410462
82 rdf:type schema:PropertyValue
83 N299aa623680d4b53a380dc39e23e56fd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Hedgehog Proteins
85 rdf:type schema:DefinedTerm
86 N318486309ec1400aae94b9725f1bd785 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Calcium Channels
88 rdf:type schema:DefinedTerm
89 N4257df4c1c704557a1953435fa41383b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Mice
91 rdf:type schema:DefinedTerm
92 N428b2f99de90487586bfc72bb4cc7e9c rdf:first sg:person.0672201126.59
93 rdf:rest rdf:nil
94 N5039f20cab874e759ed7a06ef9e50426 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Receptors, Cell Surface
96 rdf:type schema:DefinedTerm
97 N5875219efaa74079a4a8f85c49936621 schema:name readcube_id
98 schema:value 6f0464825814db0a71e509c92312ed58a7979584967fb014dbf5b4f36734417f
99 rdf:type schema:PropertyValue
100 N5b275b91c33941a290d835c9e2c71476 schema:name pubmed_id
101 schema:value 24336288
102 rdf:type schema:PropertyValue
103 N6f17a8618f0e42e9a845038881a0dd79 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Cytoplasm
105 rdf:type schema:DefinedTerm
106 N7ced153a574c4177af9275de1c6240d4 rdf:first sg:person.0725133462.06
107 rdf:rest N9b7905023ebf45b0a0c6b4bde9dee043
108 N7cf237dedc8b45988e66f34424d34368 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Cilia
110 rdf:type schema:DefinedTerm
111 N821b7ea275f64f529c162d78daf97030 schema:issueNumber 7479
112 rdf:type schema:PublicationIssue
113 N8c724a0677144ab4a8e55f5b2532e8b2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Organelles
115 rdf:type schema:DefinedTerm
116 N8fdfd0c322494472a8e9c76126c83ff8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Smoothened Receptor
118 rdf:type schema:DefinedTerm
119 N9602ccf5181247ffae00a8ca8f8ed80b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Animals
121 rdf:type schema:DefinedTerm
122 N9b7905023ebf45b0a0c6b4bde9dee043 rdf:first sg:person.01024301701.91
123 rdf:rest N428b2f99de90487586bfc72bb4cc7e9c
124 N9bf542b177864166ad3587855bba445f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Female
126 rdf:type schema:DefinedTerm
127 N9d907d74912d467eaad1a01a82a97629 rdf:first sg:person.01012045614.13
128 rdf:rest Nc8ae70cb57f34dc2b12d30d2d55567be
129 Na25437586e0243c0bb193db8229a7276 schema:name dimensions_id
130 schema:value pub.1023302129
131 rdf:type schema:PropertyValue
132 Na2846660669247fd99a1ab6500c1bec2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Calcium Signaling
134 rdf:type schema:DefinedTerm
135 Nb4062dbd35b14721905baf5eeaa201b8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Cells, Cultured
137 rdf:type schema:DefinedTerm
138 Nb6071e1cc54642f3944e53a99907de6f schema:name doi
139 schema:value 10.1038/nature12833
140 rdf:type schema:PropertyValue
141 Nb6e413d58f7a442191170d814ac746c8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Calcium
143 rdf:type schema:DefinedTerm
144 Nbc22feafc8f14bdf9bbfa4298aa5b4eb schema:volumeNumber 504
145 rdf:type schema:PublicationVolume
146 Nc8ae70cb57f34dc2b12d30d2d55567be rdf:first sg:person.01060161014.72
147 rdf:rest N7ced153a574c4177af9275de1c6240d4
148 Ncc5ab08c0def4ba7869044036e6eff16 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Kruppel-Like Transcription Factors
150 rdf:type schema:DefinedTerm
151 Nd89e3568f34e41818c3b3183d349a726 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Zinc Finger Protein Gli2
153 rdf:type schema:DefinedTerm
154 Ndd397bf18fb7465288416c8caa003ef3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Nuclear Proteins
156 rdf:type schema:DefinedTerm
157 Ne7d813eed97e467c9ca549d4a6892a46 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Male
159 rdf:type schema:DefinedTerm
160 Neaf0928bc66d48ce93c44040021e9fc0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
161 schema:name Receptors, G-Protein-Coupled
162 rdf:type schema:DefinedTerm
163 Nf1fc3df24aca4d7d8b36ce852ca9f190 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
164 schema:name Zinc Finger Protein GLI1
165 rdf:type schema:DefinedTerm
166 Nf2b75a20f1064bf480e580bff92e898a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
167 schema:name Membrane Proteins
168 rdf:type schema:DefinedTerm
169 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
170 schema:name Biological Sciences
171 rdf:type schema:DefinedTerm
172 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
173 schema:name Biochemistry and Cell Biology
174 rdf:type schema:DefinedTerm
175 sg:grant.2437483 http://pending.schema.org/fundedItem sg:pub.10.1038/nature12833
176 rdf:type schema:MonetaryGrant
177 sg:grant.2439252 http://pending.schema.org/fundedItem sg:pub.10.1038/nature12833
178 rdf:type schema:MonetaryGrant
179 sg:grant.2684748 http://pending.schema.org/fundedItem sg:pub.10.1038/nature12833
180 rdf:type schema:MonetaryGrant
181 sg:journal.1018957 schema:issn 0090-0028
182 1476-4687
183 schema:name Nature
184 rdf:type schema:Periodical
185 sg:person.01012045614.13 schema:affiliation https://www.grid.ac/institutes/grid.2515.3
186 schema:familyName Delling
187 schema:givenName Markus
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01012045614.13
189 rdf:type schema:Person
190 sg:person.01024301701.91 schema:affiliation https://www.grid.ac/institutes/grid.2515.3
191 schema:familyName Febvay
192 schema:givenName Sebastien
193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01024301701.91
194 rdf:type schema:Person
195 sg:person.01060161014.72 schema:affiliation https://www.grid.ac/institutes/grid.2515.3
196 schema:familyName DeCaen
197 schema:givenName Paul G.
198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01060161014.72
199 rdf:type schema:Person
200 sg:person.0672201126.59 schema:affiliation https://www.grid.ac/institutes/grid.38142.3c
201 schema:familyName Clapham
202 schema:givenName David E.
203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0672201126.59
204 rdf:type schema:Person
205 sg:person.0725133462.06 schema:affiliation https://www.grid.ac/institutes/grid.2515.3
206 schema:familyName Doerner
207 schema:givenName Julia F.
208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0725133462.06
209 rdf:type schema:Person
210 sg:pub.10.1007/978-1-4615-0067-4_44 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047319687
211 https://doi.org/10.1007/978-1-4615-0067-4_44
212 rdf:type schema:CreativeWork
213 sg:pub.10.1007/s00232-001-0075-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037575656
214 https://doi.org/10.1007/s00232-001-0075-4
215 rdf:type schema:CreativeWork
216 sg:pub.10.1007/s00383-010-2622-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023230919
217 https://doi.org/10.1007/s00383-010-2622-5
218 rdf:type schema:CreativeWork
219 sg:pub.10.1038/nature04117 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048565750
220 https://doi.org/10.1038/nature04117
221 rdf:type schema:CreativeWork
222 sg:pub.10.1038/nature08895 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016398543
223 https://doi.org/10.1038/nature08895
224 rdf:type schema:CreativeWork
225 sg:pub.10.1038/nature12832 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010806074
226 https://doi.org/10.1038/nature12832
227 rdf:type schema:CreativeWork
228 sg:pub.10.1038/nmeth.1398 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035553144
229 https://doi.org/10.1038/nmeth.1398
230 rdf:type schema:CreativeWork
231 sg:pub.10.1038/nn.2928 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009160296
232 https://doi.org/10.1038/nn.2928
233 rdf:type schema:CreativeWork
234 sg:pub.10.1038/nn1698 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046380817
235 https://doi.org/10.1038/nn1698
236 rdf:type schema:CreativeWork
237 sg:pub.10.1038/nrg2774 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047735807
238 https://doi.org/10.1038/nrg2774
239 rdf:type schema:CreativeWork
240 https://app.dimensions.ai/details/publication/pub.1074643455 schema:CreativeWork
241 https://doi.org/10.1016/j.cell.2007.11.028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041047694
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1016/j.cub.2013.02.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048566772
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1016/j.devcel.2012.04.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030047319
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1038/emboj.2011.110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037015123
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1073/pnas.0505328102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023197118
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1073/pnas.0912180106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002649166
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1083/jcb.201004108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037335995
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1093/bioinformatics/btp184 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047735637
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1101/gad.1902910 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031351817
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1126/science.1208592 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049468469
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1126/stke.2192004pl5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008052869
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1177/0300985809353553 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051976376
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1242/dev.058149 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053246160
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1371/journal.pone.0020007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024505509
268 rdf:type schema:CreativeWork
269 https://www.grid.ac/institutes/grid.2515.3 schema:alternateName Boston Children's Hospital
270 schema:name Howard Hughes Medical Institute, Department of Cardiology, Boston Children’s Hospital, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
271 rdf:type schema:Organization
272 https://www.grid.ac/institutes/grid.38142.3c schema:alternateName Harvard University
273 schema:name Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA
274 Howard Hughes Medical Institute, Department of Cardiology, Boston Children’s Hospital, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
275 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...