Structural basis for ligase-specific conjugation of linear ubiquitin chains by HOIP View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2013-11

AUTHORS

Benjamin Stieglitz, Rohini R. Rana, Marios G. Koliopoulos, Aylin C. Morris-Davies, Veronique Schaeffer, Evangelos Christodoulou, Steven Howell, Nicholas R. Brown, Ivan Dikic, Katrin Rittinger

ABSTRACT

Linear ubiquitin chains are important regulators of cellular signalling pathways that control innate immunity and inflammation through nuclear factor (NF)-κB activation and protection against tumour necrosis factor-α-induced apoptosis. They are synthesized by HOIP, which belongs to the RBR (RING-between-RING) family of E3 ligases and is the catalytic component of LUBAC (linear ubiquitin chain assembly complex), a multisubunit E3 ligase. RBR family members act as RING/HECT hybrids, employing RING1 to recognize ubiquitin-loaded E2 while a conserved cysteine in RING2 subsequently forms a thioester intermediate with the transferred or 'donor' ubiquitin. Here we report the crystal structure of the catalytic core of HOIP in its apo form and in complex with ubiquitin. The carboxy-terminal portion of HOIP adopts a novel fold that, together with a zinc-finger, forms a ubiquitin-binding platform that orients the acceptor ubiquitin and positions its α-amino group for nucleophilic attack on the E3∼ubiquitin thioester. The C-terminal tail of a second ubiquitin molecule is located in close proximity to the catalytic cysteine, providing a unique snapshot of the ubiquitin transfer complex containing both donor and acceptor ubiquitin. These interactions are required for activation of the NF-κB pathway in vivo, and they explain the determinants of linear ubiquitin chain specificity by LUBAC. More... »

PAGES

422

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nature12638

DOI

http://dx.doi.org/10.1038/nature12638

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010223778

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24141947


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Download the RDF metadata as:  json-ld nt turtle xml License info

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N-Triples is a line-based linked data format ideal for batch operations.

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Turtle is a human-readable linked data format.

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RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature12638'


 

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