Ultrasensitive fluorescent proteins for imaging neuronal activity View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2013-07-17

AUTHORS

Tsai-Wen Chen, Trevor J. Wardill, Yi Sun, Stefan R. Pulver, Sabine L. Renninger, Amy Baohan, Eric R. Schreiter, Rex A. Kerr, Michael B. Orger, Vivek Jayaraman, Loren L. Looger, Karel Svoboda, Douglas S. Kim

ABSTRACT

Fluorescent calcium sensors are widely used to image neural activity. Using structure-based mutagenesis and neuron-based screening, we developed a family of ultrasensitive protein calcium sensors (GCaMP6) that outperformed other sensors in cultured neurons and in zebrafish, flies and mice in vivo. In layer 2/3 pyramidal neurons of the mouse visual cortex, GCaMP6 reliably detected single action potentials in neuronal somata and orientation-tuned synaptic calcium transients in individual dendritic spines. The orientation tuning of structurally persistent spines was largely stable over timescales of weeks. Orientation tuning averaged across spine populations predicted the tuning of their parent cell. Although the somata of GABAergic neurons showed little orientation tuning, their dendrites included highly tuned dendritic segments (5–40-µm long). GCaMP6 sensors thus provide new windows into the organization and dynamics of neural circuits over multiple spatial and temporal scales. More... »

PAGES

295-300

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  • Journal

    TITLE

    Nature

    ISSUE

    7458

    VOLUME

    499

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nature12354

    DOI

    http://dx.doi.org/10.1038/nature12354

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1043516362

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/23868258


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