Ultrasensitive fluorescent proteins for imaging neuronal activity View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2013-07-17

AUTHORS

Tsai-Wen Chen, Trevor J. Wardill, Yi Sun, Stefan R. Pulver, Sabine L. Renninger, Amy Baohan, Eric R. Schreiter, Rex A. Kerr, Michael B. Orger, Vivek Jayaraman, Loren L. Looger, Karel Svoboda, Douglas S. Kim

ABSTRACT

Fluorescent calcium sensors are widely used to image neural activity. Using structure-based mutagenesis and neuron-based screening, we developed a family of ultrasensitive protein calcium sensors (GCaMP6) that outperformed other sensors in cultured neurons and in zebrafish, flies and mice in vivo. In layer 2/3 pyramidal neurons of the mouse visual cortex, GCaMP6 reliably detected single action potentials in neuronal somata and orientation-tuned synaptic calcium transients in individual dendritic spines. The orientation tuning of structurally persistent spines was largely stable over timescales of weeks. Orientation tuning averaged across spine populations predicted the tuning of their parent cell. Although the somata of GABAergic neurons showed little orientation tuning, their dendrites included highly tuned dendritic segments (5–40-µm long). GCaMP6 sensors thus provide new windows into the organization and dynamics of neural circuits over multiple spatial and temporal scales. More... »

PAGES

295-300

References to SciGraph publications

  • 2002-12. Long-term in vivo imaging of experience-dependent synaptic plasticity in adult cortex in NATURE
  • 2012-04-25. Multiple dynamic representations in the motor cortex during sensorimotor learning in NATURE
  • 1997-08. Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin in NATURE
  • 2011-11-27. Optical recording of action potentials in mammalian neurons using a microbial rhodopsin in NATURE METHODS
  • 2001-02. A high signal-to-noise Ca2+ probe composed of a single green fluorescent protein in NATURE BIOTECHNOLOGY
  • 2003-05-17. ScanImage: Flexible software for operating laser scanning microscopes in BIOMEDICAL ENGINEERING ONLINE
  • 2012-11-11. Nonlinear dendritic integration of sensory and motor input during an active sensing task in NATURE
  • 2005-08-14. Heterogeneity in synaptic transmission along a Drosophila larval motor axon in NATURE NEUROSCIENCE
  • 2010-08-08. Spontaneous network activity visualized by ultrasensitive Ca2+ indicators, yellow Cameleon-Nano in NATURE METHODS
  • 2004-11-21. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein in NATURE BIOTECHNOLOGY
  • 2009-04-12. Enzymatic assembly of DNA molecules up to several hundred kilobases in NATURE METHODS
  • 2011-07-17. Differential connectivity and response dynamics of excitatory and inhibitory neurons in visual cortex in NATURE NEUROSCIENCE
  • 2011-03-09. Network anatomy and in vivo physiology of visual cortical neurons in NATURE
  • 1999-05. Synaptic calcium transients in single spines indicate that NMDA receptors are not saturated in NATURE
  • 2011-04-10. Functional specificity of local synaptic connections in neocortical networks in NATURE
  • 2009-11-08. A genetically encoded reporter of synaptic activity in vivo in NATURE METHODS
  • 2009-11-08. Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators in NATURE METHODS
  • 2012-08-26. Activity in motor–sensory projections reveals distributed coding in somatosensation in NATURE
  • 2010-01-31. Dichotomy of functional organization in the mouse auditory cortex in NATURE NEUROSCIENCE
  • 1997-01. In vivo dendritic calcium dynamics in neocortical pyramidal neurons in NATURE
  • 2010-04-01. Dendritic organization of sensory input to cortical neurons in vivo in NATURE
  • 2011-06-26. Functional mapping of single spines in cortical neurons in vivo in NATURE
  • 2005-01-19. Functional imaging with cellular resolution reveals precise micro-architecture in visual cortex in NATURE
  • 2008-08-10. A genetically encoded calcium indicator for chronic in vivo two-photon imaging in NATURE METHODS
  • Journal

    TITLE

    Nature

    ISSUE

    7458

    VOLUME

    499

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nature12354

    DOI

    http://dx.doi.org/10.1038/nature12354

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1043516362

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/23868258


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