Natural RNA circles function as efficient microRNA sponges View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2013-03

AUTHORS

Thomas B. Hansen, Trine I. Jensen, Bettina H. Clausen, Jesper B. Bramsen, Bente Finsen, Christian K. Damgaard, Jørgen Kjems

ABSTRACT

MicroRNAs (miRNAs) are important post-transcriptional regulators of gene expression that act by direct base pairing to target sites within untranslated regions of messenger RNAs. Recently, miRNA activity has been shown to be affected by the presence of miRNA sponge transcripts, the so-called competing endogenous RNA in humans and target mimicry in plants. We previously identified a highly expressed circular RNA (circRNA) in human and mouse brain. Here we show that this circRNA acts as a miR-7 sponge; we term this circular transcript ciRS-7 (circular RNA sponge for miR-7). ciRS-7 contains more than 70 selectively conserved miRNA target sites, and it is highly and widely associated with Argonaute (AGO) proteins in a miR-7-dependent manner. Although the circRNA is completely resistant to miRNA-mediated target destabilization, it strongly suppresses miR-7 activity, resulting in increased levels of miR-7 targets. In the mouse brain, we observe overlapping co-expression of ciRS-7 and miR-7, particularly in neocortical and hippocampal neurons, suggesting a high degree of endogenous interaction. We further show that the testis-specific circRNA, sex-determining region Y (Sry), serves as a miR-138 sponge, suggesting that miRNA sponge effects achieved by circRNA formation are a general phenomenon. This study serves as the first, to our knowledge, functional analysis of a naturally expressed circRNA. More... »

PAGES

384

Journal

TITLE

Nature

ISSUE

7441

VOLUME

495

Related Patents

  • Modified Polynucleotides For The Production Of Secreted Proteins
  • Removal Of Dna Fragments In Mrna Production Process
  • Circular Polynucleotides
  • Modified Polynucleotides Encoding Granulysin
  • Modified Polynucleotides For The Production Of Biologics And Proteins Associated With Human Disease
  • Modified Polynucleotides For Treating Galactosylceramidase Protein Deficiency
  • Circular Rna For The Diagnosis Of Cardiovascular And Inflammatory Diseases
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Modified Polynucleotides Encoding Apoptosis Inducing Factor 1
  • Delivery And Formulation Of Engineered Nucleic Acids
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Modified Polynucleotides Encoding Cited4
  • Modified Polynucleotides For The Production Of Nuclear Proteins
  • Modified Polynucleotides Encoding Septin-4
  • Modified Polynucleotides For Treating Protein Deficiency
  • Modified Polynucleotides For The Production Of Proteins Associated With Human Disease
  • Delivery And Formulation Of Engineered Nucleic Acids
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Modified Polynucleotides Encoding Copper Metabolism (Murr1) Domain Containing 1
  • Modified Polynucleotides For The Production Of Proteins
  • Formulation And Delivery Of Plga Microspheres
  • Compositions And Methods Of Altering Cholesterol Levels
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Modified Polynucleotides Encoding Basic Helix-Loop-Helix Family Member E41
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Modified Polynucleotides For The Production Of Oncology-Related Proteins And Peptides
  • Circular Rna For Inhibition Of Microrna
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • A Method For Diagnosing A Disease By Detection Of Circrna In Bodily Fluids
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Terminally Modified Rna
  • Circular Rna For The Diagnosis And Treatment Of Cardiovascular Diseases
  • Modified Polynucleotides For The Production Of Cosmetic Proteins And Peptides
  • In Vivo Production Of Proteins
  • Modified Polynucleotides For The Production Of Secreted Proteins
  • Modified Polynucleotides Encoding Siah E3 Ubiquitin Protein Ligase 1
  • Modified Polynucleotides Encoding Hepatitis A Virus Cellular Receptor 2
  • Modified Polynucleotides For The Production Of Proteins
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Modified Polynucleotides Encoding Aquaporin-5
  • Modified Polynucleotides For The Production Of Proteins Associated With Blood And Lymphatic Disorders
  • Modified Polynucleotides For The Production Of Cytoplasmic And Cytoskeletal Proteins
  • Modified Polynucleotides For The Production Of Cytoplasmic And Cytoskeletal Proteins
  • Modified Polynucleotides Encoding Aryl Hydrocarbon Receptor Nuclear Translocator
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Modified Polynucleotides For The Production Of Proteins Associated With Blood And Lymphatic Disorders
  • Split Dose Administration
  • Formulation And Delivery Of Plga Microspheres
  • Modified Polynucleotides For The Production Of Biologics And Proteins Associated With Human Disease
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nature11993

    DOI

    http://dx.doi.org/10.1038/nature11993

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1050833467

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/23446346


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Argonaute Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Brain", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression Regulation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "HEK293 Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "HeLa Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Male", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice, Inbred C57BL", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "MicroRNAs", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "RNA", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sex-Determining Region Y Protein", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Aarhus University", 
              "id": "https://www.grid.ac/institutes/grid.7048.b", 
              "name": [
                "Department of Molecular Biology and Genetics, Aarhus University, C.F. M\u00f8llers Alle 3, 8000C, Aarhus, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hansen", 
            "givenName": "Thomas B.", 
            "id": "sg:person.0720515064.53", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0720515064.53"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Aarhus University", 
              "id": "https://www.grid.ac/institutes/grid.7048.b", 
              "name": [
                "Department of Molecular Biology and Genetics, Aarhus University, C.F. M\u00f8llers Alle 3, 8000C, Aarhus, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jensen", 
            "givenName": "Trine I.", 
            "id": "sg:person.0606314447.72", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0606314447.72"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Southern Denmark", 
              "id": "https://www.grid.ac/institutes/grid.10825.3e", 
              "name": [
                "Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, J.B. Winsl\u00f8wsvej 25, 5000C, Odense, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Clausen", 
            "givenName": "Bettina H.", 
            "id": "sg:person.01105541111.00", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105541111.00"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Aarhus University", 
              "id": "https://www.grid.ac/institutes/grid.7048.b", 
              "name": [
                "Department of Molecular Biology and Genetics, Aarhus University, C.F. M\u00f8llers Alle 3, 8000C, Aarhus, Denmark", 
                "Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav\u2009Wiedsvej 14, 8000C, Aarhus, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bramsen", 
            "givenName": "Jesper B.", 
            "id": "sg:person.01122005666.89", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01122005666.89"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Southern Denmark", 
              "id": "https://www.grid.ac/institutes/grid.10825.3e", 
              "name": [
                "Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, J.B. Winsl\u00f8wsvej 25, 5000C, Odense, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Finsen", 
            "givenName": "Bente", 
            "id": "sg:person.01021713024.78", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021713024.78"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Aarhus University", 
              "id": "https://www.grid.ac/institutes/grid.7048.b", 
              "name": [
                "Department of Molecular Biology and Genetics, Aarhus University, C.F. M\u00f8llers Alle 3, 8000C, Aarhus, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Damgaard", 
            "givenName": "Christian K.", 
            "id": "sg:person.01153372710.20", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153372710.20"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Aarhus University", 
              "id": "https://www.grid.ac/institutes/grid.7048.b", 
              "name": [
                "Department of Molecular Biology and Genetics, Aarhus University, C.F. M\u00f8llers Alle 3, 8000C, Aarhus, Denmark", 
                "Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav\u2009Wiedsvej 14, 8000C, Aarhus, Denmark"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kjems", 
            "givenName": "J\u00f8rgen", 
            "id": "sg:person.01100525057.10", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01100525057.10"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/ng2079", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000182181", 
              "https://doi.org/10.1038/ng2079"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/emboj/20.23.6877", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004775429"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(95)00639-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005590983"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj20100859", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008685778"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj20100859", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008685778"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj20100859", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008685778"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(93)90279-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008757749"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0030733", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008798616"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0030733", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008798616"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncb1274", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014076938", 
              "https://doi.org/10.1038/ncb1274"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncb1274", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014076938", 
              "https://doi.org/10.1038/ncb1274"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gad.1282305", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015909772"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00221-006-0526-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016720226", 
              "https://doi.org/10.1007/s00221-006-0526-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00221-006-0526-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016720226", 
              "https://doi.org/10.1007/s00221-006-0526-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1158/0008-5472.can-08-2103", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016862117"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2011.09.041", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019142569"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2009.01.002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023682432"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth1079", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028333288", 
              "https://doi.org/10.1038/nmeth1079"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkq1316", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034030947"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1742-2094-5-46", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036279804", 
              "https://doi.org/10.1186/1742-2094-5-46"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1261/rna.783108", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038932587"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0906277106", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040496385"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2011.09.028", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041816546"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2011.09.032", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044541376"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature08170", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045137706", 
              "https://doi.org/10.1038/nature08170"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature08170", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045137706", 
              "https://doi.org/10.1038/nature08170"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1038/emboj.2011.359", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046169131"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1158/0008-5472.can-07-6639", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046600339"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1371/journal.pone.0002126", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049237260"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature09144", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050391113", 
              "https://doi.org/10.1038/nature09144"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature09144", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050391113", 
              "https://doi.org/10.1038/nature09144"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2011.09.029", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053383331"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1073827", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062446723"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082424111", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082924860", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2013-03", 
        "datePublishedReg": "2013-03-01", 
        "description": "MicroRNAs (miRNAs) are important post-transcriptional regulators of gene expression that act by direct base pairing to target sites within untranslated regions of messenger RNAs. Recently, miRNA activity has been shown to be affected by the presence of miRNA sponge transcripts, the so-called competing endogenous RNA in humans and target mimicry in plants. We previously identified a highly expressed circular RNA (circRNA) in human and mouse brain. Here we show that this circRNA acts as a miR-7 sponge; we term this circular transcript ciRS-7 (circular RNA sponge for miR-7). ciRS-7 contains more than 70 selectively conserved miRNA target sites, and it is highly and widely associated with Argonaute (AGO) proteins in a miR-7-dependent manner. Although the circRNA is completely resistant to miRNA-mediated target destabilization, it strongly suppresses miR-7 activity, resulting in increased levels of miR-7 targets. In the mouse brain, we observe overlapping co-expression of ciRS-7 and miR-7, particularly in neocortical and hippocampal neurons, suggesting a high degree of endogenous interaction. We further show that the testis-specific circRNA, sex-determining region Y (Sry), serves as a miR-138 sponge, suggesting that miRNA sponge effects achieved by circRNA formation are a general phenomenon. This study serves as the first, to our knowledge, functional analysis of a naturally expressed circRNA.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nature11993", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1018957", 
            "issn": [
              "0090-0028", 
              "1476-4687"
            ], 
            "name": "Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "7441", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "495"
          }
        ], 
        "name": "Natural RNA circles function as efficient microRNA sponges", 
        "pagination": "384", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "f2268c5ee977457f536be55d03f7964f94f888bb3463c876a82ab8d77c80ce69"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "23446346"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "0410462"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nature11993"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1050833467"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nature11993", 
          "https://app.dimensions.ai/details/publication/pub.1050833467"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T17:19", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8672_00000426.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://www.nature.com/articles/nature11993"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature11993'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature11993'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature11993'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature11993'


     

    This table displays all metadata directly associated to this object as RDF triples.

    256 TRIPLES      21 PREDICATES      70 URIs      34 LITERALS      22 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nature11993 schema:about N0194c9b2292f4dee9bd3cab97dfff90b
    2 N11b5a81361084967bfcf04d4495a7cab
    3 N41cc7eb416094c6a919450b717f349d0
    4 N468f7ce62e0547418ff75942b38cbf04
    5 N519bddcc31c04844a2dc0f6e7b84491c
    6 N61e8ba3e0f98479cb115e52e8983b8a5
    7 N7494f270ec1a4c6ebe7f305fa9f294ac
    8 N8d496d1e6384497db59abe9eba339731
    9 Nb4a03c5037c5428bb194667e3a0c57f8
    10 Nb9cf5be6d0c44c54b40e21b8c458bfb9
    11 Nd62bdc0abae64a6d996267e4a5b503bf
    12 Ndc0e5bb291fe4a31877ed1f62b395030
    13 Neef3ba532e3f418883b3f8541b6102e8
    14 anzsrc-for:06
    15 anzsrc-for:0604
    16 schema:author Nd0b7753dae854d9996b84619abf2e928
    17 schema:citation sg:pub.10.1007/s00221-006-0526-3
    18 sg:pub.10.1038/nature08170
    19 sg:pub.10.1038/nature09144
    20 sg:pub.10.1038/ncb1274
    21 sg:pub.10.1038/ng2079
    22 sg:pub.10.1038/nmeth1079
    23 sg:pub.10.1186/1742-2094-5-46
    24 https://app.dimensions.ai/details/publication/pub.1082424111
    25 https://app.dimensions.ai/details/publication/pub.1082924860
    26 https://doi.org/10.1016/0092-8674(93)90279-y
    27 https://doi.org/10.1016/0378-1119(95)00639-7
    28 https://doi.org/10.1016/j.cell.2009.01.002
    29 https://doi.org/10.1016/j.cell.2011.09.028
    30 https://doi.org/10.1016/j.cell.2011.09.029
    31 https://doi.org/10.1016/j.cell.2011.09.032
    32 https://doi.org/10.1016/j.cell.2011.09.041
    33 https://doi.org/10.1038/emboj.2011.359
    34 https://doi.org/10.1042/bj20100859
    35 https://doi.org/10.1073/pnas.0906277106
    36 https://doi.org/10.1093/emboj/20.23.6877
    37 https://doi.org/10.1093/nar/gkq1316
    38 https://doi.org/10.1101/gad.1282305
    39 https://doi.org/10.1126/science.1073827
    40 https://doi.org/10.1158/0008-5472.can-07-6639
    41 https://doi.org/10.1158/0008-5472.can-08-2103
    42 https://doi.org/10.1261/rna.783108
    43 https://doi.org/10.1371/journal.pone.0002126
    44 https://doi.org/10.1371/journal.pone.0030733
    45 schema:datePublished 2013-03
    46 schema:datePublishedReg 2013-03-01
    47 schema:description MicroRNAs (miRNAs) are important post-transcriptional regulators of gene expression that act by direct base pairing to target sites within untranslated regions of messenger RNAs. Recently, miRNA activity has been shown to be affected by the presence of miRNA sponge transcripts, the so-called competing endogenous RNA in humans and target mimicry in plants. We previously identified a highly expressed circular RNA (circRNA) in human and mouse brain. Here we show that this circRNA acts as a miR-7 sponge; we term this circular transcript ciRS-7 (circular RNA sponge for miR-7). ciRS-7 contains more than 70 selectively conserved miRNA target sites, and it is highly and widely associated with Argonaute (AGO) proteins in a miR-7-dependent manner. Although the circRNA is completely resistant to miRNA-mediated target destabilization, it strongly suppresses miR-7 activity, resulting in increased levels of miR-7 targets. In the mouse brain, we observe overlapping co-expression of ciRS-7 and miR-7, particularly in neocortical and hippocampal neurons, suggesting a high degree of endogenous interaction. We further show that the testis-specific circRNA, sex-determining region Y (Sry), serves as a miR-138 sponge, suggesting that miRNA sponge effects achieved by circRNA formation are a general phenomenon. This study serves as the first, to our knowledge, functional analysis of a naturally expressed circRNA.
    48 schema:genre research_article
    49 schema:inLanguage en
    50 schema:isAccessibleForFree false
    51 schema:isPartOf N191691b0df484415a7ba973d53052eb1
    52 N7be311da3f6946d6bc228daa2f0cb651
    53 sg:journal.1018957
    54 schema:name Natural RNA circles function as efficient microRNA sponges
    55 schema:pagination 384
    56 schema:productId N1a2abaede9974badbc46f2bc800246cf
    57 N395bac565d3a49b1abeb28aad962ca68
    58 Na9c08dbbd9c24d0293db6d022870efb4
    59 Nb3dcd6fb025e43c08c7d6f224f4c6b0b
    60 Nd1dc7c0b9d0e4f989f40cacc125ccf10
    61 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050833467
    62 https://doi.org/10.1038/nature11993
    63 schema:sdDatePublished 2019-04-10T17:19
    64 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    65 schema:sdPublisher Ndf3ff0e3134041059f70335dc872feac
    66 schema:url https://www.nature.com/articles/nature11993
    67 sgo:license sg:explorer/license/
    68 sgo:sdDataset articles
    69 rdf:type schema:ScholarlyArticle
    70 N0194c9b2292f4dee9bd3cab97dfff90b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    71 schema:name Sex-Determining Region Y Protein
    72 rdf:type schema:DefinedTerm
    73 N11b5a81361084967bfcf04d4495a7cab schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    74 schema:name Mice, Inbred C57BL
    75 rdf:type schema:DefinedTerm
    76 N191691b0df484415a7ba973d53052eb1 schema:issueNumber 7441
    77 rdf:type schema:PublicationIssue
    78 N1a2abaede9974badbc46f2bc800246cf schema:name dimensions_id
    79 schema:value pub.1050833467
    80 rdf:type schema:PropertyValue
    81 N220ad978d3b54f5197f7979d52428e6b rdf:first sg:person.01021713024.78
    82 rdf:rest N5f72bb884d4a4a448ae97e68f0f75825
    83 N2eaa5f30f1fd40c7801b557ec29286c4 rdf:first sg:person.0606314447.72
    84 rdf:rest N7d8bb3c44d844c7e82d9bb12aaeadd21
    85 N395bac565d3a49b1abeb28aad962ca68 schema:name pubmed_id
    86 schema:value 23446346
    87 rdf:type schema:PropertyValue
    88 N41cc7eb416094c6a919450b717f349d0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    89 schema:name Argonaute Proteins
    90 rdf:type schema:DefinedTerm
    91 N468f7ce62e0547418ff75942b38cbf04 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    92 schema:name HEK293 Cells
    93 rdf:type schema:DefinedTerm
    94 N519bddcc31c04844a2dc0f6e7b84491c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    95 schema:name Brain
    96 rdf:type schema:DefinedTerm
    97 N5f72bb884d4a4a448ae97e68f0f75825 rdf:first sg:person.01153372710.20
    98 rdf:rest Nf919a0d58032479899821610ba469c4f
    99 N61e8ba3e0f98479cb115e52e8983b8a5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    100 schema:name RNA
    101 rdf:type schema:DefinedTerm
    102 N7494f270ec1a4c6ebe7f305fa9f294ac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    103 schema:name Mice
    104 rdf:type schema:DefinedTerm
    105 N7be311da3f6946d6bc228daa2f0cb651 schema:volumeNumber 495
    106 rdf:type schema:PublicationVolume
    107 N7d8bb3c44d844c7e82d9bb12aaeadd21 rdf:first sg:person.01105541111.00
    108 rdf:rest N8261a46f18a74d048ed79cf37378c320
    109 N8261a46f18a74d048ed79cf37378c320 rdf:first sg:person.01122005666.89
    110 rdf:rest N220ad978d3b54f5197f7979d52428e6b
    111 N8d496d1e6384497db59abe9eba339731 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    112 schema:name Male
    113 rdf:type schema:DefinedTerm
    114 Na9c08dbbd9c24d0293db6d022870efb4 schema:name doi
    115 schema:value 10.1038/nature11993
    116 rdf:type schema:PropertyValue
    117 Nb3dcd6fb025e43c08c7d6f224f4c6b0b schema:name readcube_id
    118 schema:value f2268c5ee977457f536be55d03f7964f94f888bb3463c876a82ab8d77c80ce69
    119 rdf:type schema:PropertyValue
    120 Nb4a03c5037c5428bb194667e3a0c57f8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    121 schema:name Animals
    122 rdf:type schema:DefinedTerm
    123 Nb9cf5be6d0c44c54b40e21b8c458bfb9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    124 schema:name Gene Expression Regulation
    125 rdf:type schema:DefinedTerm
    126 Nd0b7753dae854d9996b84619abf2e928 rdf:first sg:person.0720515064.53
    127 rdf:rest N2eaa5f30f1fd40c7801b557ec29286c4
    128 Nd1dc7c0b9d0e4f989f40cacc125ccf10 schema:name nlm_unique_id
    129 schema:value 0410462
    130 rdf:type schema:PropertyValue
    131 Nd62bdc0abae64a6d996267e4a5b503bf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    132 schema:name HeLa Cells
    133 rdf:type schema:DefinedTerm
    134 Ndc0e5bb291fe4a31877ed1f62b395030 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    135 schema:name MicroRNAs
    136 rdf:type schema:DefinedTerm
    137 Ndf3ff0e3134041059f70335dc872feac schema:name Springer Nature - SN SciGraph project
    138 rdf:type schema:Organization
    139 Neef3ba532e3f418883b3f8541b6102e8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    140 schema:name Humans
    141 rdf:type schema:DefinedTerm
    142 Nf919a0d58032479899821610ba469c4f rdf:first sg:person.01100525057.10
    143 rdf:rest rdf:nil
    144 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    145 schema:name Biological Sciences
    146 rdf:type schema:DefinedTerm
    147 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    148 schema:name Genetics
    149 rdf:type schema:DefinedTerm
    150 sg:journal.1018957 schema:issn 0090-0028
    151 1476-4687
    152 schema:name Nature
    153 rdf:type schema:Periodical
    154 sg:person.01021713024.78 schema:affiliation https://www.grid.ac/institutes/grid.10825.3e
    155 schema:familyName Finsen
    156 schema:givenName Bente
    157 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01021713024.78
    158 rdf:type schema:Person
    159 sg:person.01100525057.10 schema:affiliation https://www.grid.ac/institutes/grid.7048.b
    160 schema:familyName Kjems
    161 schema:givenName Jørgen
    162 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01100525057.10
    163 rdf:type schema:Person
    164 sg:person.01105541111.00 schema:affiliation https://www.grid.ac/institutes/grid.10825.3e
    165 schema:familyName Clausen
    166 schema:givenName Bettina H.
    167 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105541111.00
    168 rdf:type schema:Person
    169 sg:person.01122005666.89 schema:affiliation https://www.grid.ac/institutes/grid.7048.b
    170 schema:familyName Bramsen
    171 schema:givenName Jesper B.
    172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01122005666.89
    173 rdf:type schema:Person
    174 sg:person.01153372710.20 schema:affiliation https://www.grid.ac/institutes/grid.7048.b
    175 schema:familyName Damgaard
    176 schema:givenName Christian K.
    177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153372710.20
    178 rdf:type schema:Person
    179 sg:person.0606314447.72 schema:affiliation https://www.grid.ac/institutes/grid.7048.b
    180 schema:familyName Jensen
    181 schema:givenName Trine I.
    182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0606314447.72
    183 rdf:type schema:Person
    184 sg:person.0720515064.53 schema:affiliation https://www.grid.ac/institutes/grid.7048.b
    185 schema:familyName Hansen
    186 schema:givenName Thomas B.
    187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0720515064.53
    188 rdf:type schema:Person
    189 sg:pub.10.1007/s00221-006-0526-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016720226
    190 https://doi.org/10.1007/s00221-006-0526-3
    191 rdf:type schema:CreativeWork
    192 sg:pub.10.1038/nature08170 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045137706
    193 https://doi.org/10.1038/nature08170
    194 rdf:type schema:CreativeWork
    195 sg:pub.10.1038/nature09144 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050391113
    196 https://doi.org/10.1038/nature09144
    197 rdf:type schema:CreativeWork
    198 sg:pub.10.1038/ncb1274 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014076938
    199 https://doi.org/10.1038/ncb1274
    200 rdf:type schema:CreativeWork
    201 sg:pub.10.1038/ng2079 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000182181
    202 https://doi.org/10.1038/ng2079
    203 rdf:type schema:CreativeWork
    204 sg:pub.10.1038/nmeth1079 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028333288
    205 https://doi.org/10.1038/nmeth1079
    206 rdf:type schema:CreativeWork
    207 sg:pub.10.1186/1742-2094-5-46 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036279804
    208 https://doi.org/10.1186/1742-2094-5-46
    209 rdf:type schema:CreativeWork
    210 https://app.dimensions.ai/details/publication/pub.1082424111 schema:CreativeWork
    211 https://app.dimensions.ai/details/publication/pub.1082924860 schema:CreativeWork
    212 https://doi.org/10.1016/0092-8674(93)90279-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1008757749
    213 rdf:type schema:CreativeWork
    214 https://doi.org/10.1016/0378-1119(95)00639-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005590983
    215 rdf:type schema:CreativeWork
    216 https://doi.org/10.1016/j.cell.2009.01.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023682432
    217 rdf:type schema:CreativeWork
    218 https://doi.org/10.1016/j.cell.2011.09.028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041816546
    219 rdf:type schema:CreativeWork
    220 https://doi.org/10.1016/j.cell.2011.09.029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053383331
    221 rdf:type schema:CreativeWork
    222 https://doi.org/10.1016/j.cell.2011.09.032 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044541376
    223 rdf:type schema:CreativeWork
    224 https://doi.org/10.1016/j.cell.2011.09.041 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019142569
    225 rdf:type schema:CreativeWork
    226 https://doi.org/10.1038/emboj.2011.359 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046169131
    227 rdf:type schema:CreativeWork
    228 https://doi.org/10.1042/bj20100859 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008685778
    229 rdf:type schema:CreativeWork
    230 https://doi.org/10.1073/pnas.0906277106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040496385
    231 rdf:type schema:CreativeWork
    232 https://doi.org/10.1093/emboj/20.23.6877 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004775429
    233 rdf:type schema:CreativeWork
    234 https://doi.org/10.1093/nar/gkq1316 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034030947
    235 rdf:type schema:CreativeWork
    236 https://doi.org/10.1101/gad.1282305 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015909772
    237 rdf:type schema:CreativeWork
    238 https://doi.org/10.1126/science.1073827 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062446723
    239 rdf:type schema:CreativeWork
    240 https://doi.org/10.1158/0008-5472.can-07-6639 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046600339
    241 rdf:type schema:CreativeWork
    242 https://doi.org/10.1158/0008-5472.can-08-2103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016862117
    243 rdf:type schema:CreativeWork
    244 https://doi.org/10.1261/rna.783108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038932587
    245 rdf:type schema:CreativeWork
    246 https://doi.org/10.1371/journal.pone.0002126 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049237260
    247 rdf:type schema:CreativeWork
    248 https://doi.org/10.1371/journal.pone.0030733 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008798616
    249 rdf:type schema:CreativeWork
    250 https://www.grid.ac/institutes/grid.10825.3e schema:alternateName University of Southern Denmark
    251 schema:name Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, J.B. Winsløwsvej 25, 5000C, Odense, Denmark
    252 rdf:type schema:Organization
    253 https://www.grid.ac/institutes/grid.7048.b schema:alternateName Aarhus University
    254 schema:name Department of Molecular Biology and Genetics, Aarhus University, C.F. Møllers Alle 3, 8000C, Aarhus, Denmark
    255 Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wiedsvej 14, 8000C, Aarhus, Denmark
    256 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...