Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2011-08

AUTHORS

Wim de Lau, Nick Barker, Teck Y. Low, Bon-Kyoung Koo, Vivian S. W. Li, Hans Teunissen, Pekka Kujala, Andrea Haegebarth, Peter J. Peters, Marc van de Wetering, D. E. Stange, J. van Es, Daniele Guardavaccaro, Richard B. M. Schasfoort, Yasuaki Mohri, Katsuhiko Nishimori, Shabaz Mohammed, Albert J. R. Heck, Hans Clevers

ABSTRACT

The adult stem cell marker Lgr5 and its relative Lgr4 are often co-expressed in Wnt-driven proliferative compartments. We find that conditional deletion of both genes in the mouse gut impairs Wnt target gene expression and results in the rapid demise of intestinal crypts, thus phenocopying Wnt pathway inhibition. Mass spectrometry demonstrates that Lgr4 and Lgr5 associate with the Frizzled/Lrp Wnt receptor complex. Each of the four R-spondins, secreted Wnt pathway agonists, can bind to Lgr4, -5 and -6. In HEK293 cells, RSPO1 enhances canonical WNT signals initiated by WNT3A. Removal of LGR4 does not affect WNT3A signalling, but abrogates the RSPO1-mediated signal enhancement, a phenomenon rescued by re-expression of LGR4, -5 or -6. Genetic deletion of Lgr4/5 in mouse intestinal crypt cultures phenocopies withdrawal of Rspo1 and can be rescued by Wnt pathway activation. Lgr5 homologues are facultative Wnt receptor components that mediate Wnt signal enhancement by soluble R-spondin proteins. These results will guide future studies towards the application of R-spondins for regenerative purposes of tissues expressing Lgr5 homologues. More... »

PAGES

293

Journal

TITLE

Nature

ISSUE

7360

VOLUME

476

From Grant

  • Related Patents

  • Rsv G Protein Specific Antibodies
  • Notch Pathway Inhibition
  • Compositions And Methods For Treating And Diagnosing Cancer
  • Methods Of Treating Cancer By Administering Anti-Gpr49 Antibodies
  • Anti-Lgr5 Antibodies And Immunoconjugates
  • Compositions And Methods Relating To Induction Of Intestinal Stem Cell Homeogenesis And/Or Regeneration
  • A Rapid Quantitative Assay To Measure Cftr Function In A Primary Intestinal Culture Model
  • Treatment Of Dermal Fibrosis
  • Anti-Gpr49 Monoclonal Antibodies
  • Liver Organoid, Uses Thereof And Culture Method For Obtaining Them
  • Methods Of Blocking Cancer Stem Cell Growth
  • Anti-Rspo2 Antibodies And Uses Thereof
  • Anti-Lgr5 Antibodies And Immunoconjugates
  • Method For Preparing Induced Paraxial Mesoderm Progenitor (Ipam) Cells And Their Use
  • Method For Identifying, Expanding, And Removing Adult Stem Cells And Cancer Stem Cells
  • Methods Of Inhibiting Solid Tumor Growth By Administering Gpr49 Antibodies
  • Culture Medium For Epithelial Stem Cells And Organoids Comprising The Stem Cells
  • A Rapid Quantitative Assay To Measure Cftr Function In A Primary Intestinal Culture Model
  • Method For Identifying, Expanding And Removing Adult Stem Cells And Cancer Stem Cells
  • Compositions And Methods For Treating And Diagnosing Cancer
  • Methods For Establishing And Improving The Survival Of A Population Of Pancreatic Progenitor Or Stem Cells
  • Rapid Quantitative Assay To Measure Cftr Function In A Primary Intestinal Culture Model
  • Rspo3 Binding Agents And Uses Thereof
  • Humanized Antibodies That Bind Lgr5
  • Methods Of Blocking Cancer Stem Cell Growth
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nature10337

    DOI

    http://dx.doi.org/10.1038/nature10337

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1053455836

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/21727895


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Adult Stem Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cells, Cultured", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Epithelial Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Frizzled Receptors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Deletion", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "HEK293 Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Binding", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Protein Structure, Tertiary", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Receptors, G-Protein-Coupled", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Regeneration", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Signal Transduction", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Thrombospondins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Wnt Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Wnt3 Protein", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Wnt3A Protein", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "name": [
                "Hubrecht Institute and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "de Lau", 
            "givenName": "Wim", 
            "id": "sg:person.01213722537.34", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01213722537.34"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Hubrecht Institute and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Barker", 
            "givenName": "Nick", 
            "id": "sg:person.01307053755.26", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307053755.26"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Utrecht University", 
              "id": "https://www.grid.ac/institutes/grid.5477.1", 
              "name": [
                "Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center, Utrecht University, 3584 CH Utrecht, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Low", 
            "givenName": "Teck Y.", 
            "id": "sg:person.01313245325.38", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313245325.38"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Hubrecht Institute and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Koo", 
            "givenName": "Bon-Kyoung", 
            "id": "sg:person.01352156370.23", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01352156370.23"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Hubrecht Institute and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Li", 
            "givenName": "Vivian S. W.", 
            "id": "sg:person.012054747172.33", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012054747172.33"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Hubrecht Institute and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Teunissen", 
            "givenName": "Hans", 
            "id": "sg:person.0677136307.17", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677136307.17"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Antoni van Leeuwenhoek Hospital Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kujala", 
            "givenName": "Pekka", 
            "id": "sg:person.0616060543.03", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0616060543.03"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Hubrecht Institute and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Haegebarth", 
            "givenName": "Andrea", 
            "id": "sg:person.01273351443.89", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01273351443.89"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Antoni van Leeuwenhoek Hospital Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Peters", 
            "givenName": "Peter J.", 
            "id": "sg:person.01132006272.03", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132006272.03"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Hubrecht Institute and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "van de Wetering", 
            "givenName": "Marc", 
            "id": "sg:person.0750240753.37", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0750240753.37"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Hubrecht Institute and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Stange", 
            "givenName": "D. E.", 
            "id": "sg:person.0643614125.13", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0643614125.13"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Hubrecht Institute and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "van Es", 
            "givenName": "J.", 
            "id": "sg:person.01064467353.67", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01064467353.67"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Hubrecht Institute and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Guardavaccaro", 
            "givenName": "Daniele", 
            "id": "sg:person.0666067727.95", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0666067727.95"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Twente", 
              "id": "https://www.grid.ac/institutes/grid.6214.1", 
              "name": [
                "Medical Cell Biophysics, MIRA institute, University of Twente, 7500 AE Enschede, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Schasfoort", 
            "givenName": "Richard B. M.", 
            "id": "sg:person.015536155047.43", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015536155047.43"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Tohoku University", 
              "id": "https://www.grid.ac/institutes/grid.69566.3a", 
              "name": [
                "Tohoku University, 981-8555 Sendai, Miyagi, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mohri", 
            "givenName": "Yasuaki", 
            "id": "sg:person.0735472007.92", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0735472007.92"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Tohoku University", 
              "id": "https://www.grid.ac/institutes/grid.69566.3a", 
              "name": [
                "Tohoku University, 981-8555 Sendai, Miyagi, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nishimori", 
            "givenName": "Katsuhiko", 
            "id": "sg:person.01151605507.23", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01151605507.23"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Utrecht University", 
              "id": "https://www.grid.ac/institutes/grid.5477.1", 
              "name": [
                "Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center, Utrecht University, 3584 CH Utrecht, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mohammed", 
            "givenName": "Shabaz", 
            "id": "sg:person.01237336020.34", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01237336020.34"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Utrecht University", 
              "id": "https://www.grid.ac/institutes/grid.5477.1", 
              "name": [
                "Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center, Utrecht University, 3584 CH Utrecht, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Heck", 
            "givenName": "Albert J. R.", 
            "id": "sg:person.07502404177.84", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07502404177.84"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Hubrecht Institute and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Clevers", 
            "givenName": "Hans", 
            "id": "sg:person.0662243670.45", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0662243670.45"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1053/j.gastro.2009.05.035", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001779262"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/1270", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002695839", 
              "https://doi.org/10.1038/1270"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/1270", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002695839", 
              "https://doi.org/10.1038/1270"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature05674", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002848303", 
              "https://doi.org/10.1038/nature05674"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1184733", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002969630"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.24.22.9736-9743.2004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003545217"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2009.01.031", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003917776"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1053/j.gastro.2007.02.001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004526083"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m701927200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007327575"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gad.267103", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011480346"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gad.267103", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011480346"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.ydbio.2009.04.020", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013884866"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.febslet.2007.08.064", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016812609"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m508324200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017083954"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m109.078261", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018759398"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1091/mbc.e08-02-0187", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022351840"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ng.239", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023194186", 
              "https://doi.org/10.1038/ng.239"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature07935", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024082069", 
              "https://doi.org/10.1038/nature07935"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature07935", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024082069", 
              "https://doi.org/10.1038/nature07935"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.devcel.2004.07.019", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024994615"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1053/j.gastro.2010.03.002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025461503"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1038/embor.2011.52", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028716824"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature09637", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030684957", 
              "https://doi.org/10.1038/nature09637"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/mcp.m800093-mcp200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031511342"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1124/mol.105.014183", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031657105"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/dvdy.20446", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031742698"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/dvdy.20446", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031742698"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0076-6879(03)70037-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035247640"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.93.16.8455", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035953957"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0506580102", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037705714"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0506580102", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037705714"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.devcel.2011.01.006", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038585487"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/pr1011729", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039087538"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2009.11.013", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039362335"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.00821-06", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040375340"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00418-005-0002-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044478492", 
              "https://doi.org/10.1007/s00418-005-0002-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00418-005-0002-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044478492", 
              "https://doi.org/10.1007/s00418-005-0002-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0702305104", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045150931"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature03659", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046707660", 
              "https://doi.org/10.1038/nature03659"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature03659", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046707660", 
              "https://doi.org/10.1038/nature03659"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.1511", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049469421", 
              "https://doi.org/10.1038/nbt.1511"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature06196", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050225537", 
              "https://doi.org/10.1038/nature06196"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.2536800100", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050279034"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.2536800100", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050279034"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1112521", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050697196"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/34848", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051110109", 
              "https://doi.org/10.1038/34848"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/34848", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051110109", 
              "https://doi.org/10.1038/34848"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/34848", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051110109", 
              "https://doi.org/10.1038/34848"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/ac950914h", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055072376"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/ac950914h", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055072376"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.275.5307.1784", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062556119"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2144/03342mt01", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1075245738"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082831306", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2011-08", 
        "datePublishedReg": "2011-08-01", 
        "description": "The adult stem cell marker Lgr5 and its relative Lgr4 are often co-expressed in Wnt-driven proliferative compartments. We find that conditional deletion of both genes in the mouse gut impairs Wnt target gene expression and results in the rapid demise of intestinal crypts, thus phenocopying Wnt pathway inhibition. Mass spectrometry demonstrates that Lgr4 and Lgr5 associate with the Frizzled/Lrp Wnt receptor complex. Each of the four R-spondins, secreted Wnt pathway agonists, can bind to Lgr4, -5 and -6. In HEK293 cells, RSPO1 enhances canonical WNT signals initiated by WNT3A. Removal of LGR4 does not affect WNT3A signalling, but abrogates the RSPO1-mediated signal enhancement, a phenomenon rescued by re-expression of LGR4, -5 or -6. Genetic deletion of Lgr4/5 in mouse intestinal crypt cultures phenocopies withdrawal of Rspo1 and can be rescued by Wnt pathway activation. Lgr5 homologues are facultative Wnt receptor components that mediate Wnt signal enhancement by soluble R-spondin proteins. These results will guide future studies towards the application of R-spondins for regenerative purposes of tissues expressing Lgr5 homologues.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nature10337", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.6058394", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.6047570", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1018957", 
            "issn": [
              "0090-0028", 
              "1476-4687"
            ], 
            "name": "Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "7360", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "476"
          }
        ], 
        "name": "Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling", 
        "pagination": "293", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "fe9de5c111a1cf3076e8fe425b2dc5c3def58db135207348b2f7c594f28bc3fe"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "21727895"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "0410462"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nature10337"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1053455836"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nature10337", 
          "https://app.dimensions.ai/details/publication/pub.1053455836"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T16:30", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8669_00000434.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://www.nature.com/articles/nature10337"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature10337'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature10337'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature10337'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature10337'


     

    This table displays all metadata directly associated to this object as RDF triples.

    438 TRIPLES      21 PREDICATES      89 URIs      39 LITERALS      27 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nature10337 schema:about N1ab492614c564633924640e69ad12690
    2 N24a4692a23ea4e91ac8fa85d2976c0c7
    3 N26e7c6934038401d8f0d4fe1f8336e1f
    4 N2b2ba15bafb24b528fa014c17dd7ace1
    5 N2d74d367311d4601a72080c3b6ac8e14
    6 N379f2954543a4b0493caa43c5060bc9f
    7 N38789e6907ca49b2965b1b0cd48fd2cd
    8 N7ed174d666e64bb7ab8b814f550862fd
    9 N833e0485eab34a6dbff2dfe95abf6491
    10 Na31720df68a04c1e9a615bf718f84cc8
    11 Naa6143a80006466d80d2fcd5783c2818
    12 Nab3e5f9906a64082886a5371c769f088
    13 Nb0a7dd555cc748deb6c28e461e4209f3
    14 Nbcb924303e194fdc899656112f4dcc37
    15 Nc70b21eb598c485ebb58a399393ad30a
    16 Nca0e8340bf1440438047bd170dd6328c
    17 Nf8f2c3455ede4029988170c6c1c3fbce
    18 Nfcaec8869c1c4b4ea13be99f6d18b5db
    19 anzsrc-for:06
    20 anzsrc-for:0601
    21 schema:author N679e45205bf742d79e284a6eceb08515
    22 schema:citation sg:pub.10.1007/s00418-005-0002-3
    23 sg:pub.10.1038/1270
    24 sg:pub.10.1038/34848
    25 sg:pub.10.1038/nature03659
    26 sg:pub.10.1038/nature05674
    27 sg:pub.10.1038/nature06196
    28 sg:pub.10.1038/nature07935
    29 sg:pub.10.1038/nature09637
    30 sg:pub.10.1038/nbt.1511
    31 sg:pub.10.1038/ng.239
    32 https://app.dimensions.ai/details/publication/pub.1082831306
    33 https://doi.org/10.1002/dvdy.20446
    34 https://doi.org/10.1016/j.cell.2009.01.031
    35 https://doi.org/10.1016/j.devcel.2004.07.019
    36 https://doi.org/10.1016/j.devcel.2011.01.006
    37 https://doi.org/10.1016/j.febslet.2007.08.064
    38 https://doi.org/10.1016/j.stem.2009.11.013
    39 https://doi.org/10.1016/j.ydbio.2009.04.020
    40 https://doi.org/10.1016/s0076-6879(03)70037-8
    41 https://doi.org/10.1021/ac950914h
    42 https://doi.org/10.1021/pr1011729
    43 https://doi.org/10.1038/embor.2011.52
    44 https://doi.org/10.1053/j.gastro.2007.02.001
    45 https://doi.org/10.1053/j.gastro.2009.05.035
    46 https://doi.org/10.1053/j.gastro.2010.03.002
    47 https://doi.org/10.1073/pnas.0506580102
    48 https://doi.org/10.1073/pnas.0702305104
    49 https://doi.org/10.1073/pnas.2536800100
    50 https://doi.org/10.1073/pnas.93.16.8455
    51 https://doi.org/10.1074/jbc.m109.078261
    52 https://doi.org/10.1074/jbc.m508324200
    53 https://doi.org/10.1074/jbc.m701927200
    54 https://doi.org/10.1074/mcp.m800093-mcp200
    55 https://doi.org/10.1091/mbc.e08-02-0187
    56 https://doi.org/10.1101/gad.267103
    57 https://doi.org/10.1124/mol.105.014183
    58 https://doi.org/10.1126/science.1112521
    59 https://doi.org/10.1126/science.1184733
    60 https://doi.org/10.1126/science.275.5307.1784
    61 https://doi.org/10.1128/mcb.00821-06
    62 https://doi.org/10.1128/mcb.24.22.9736-9743.2004
    63 https://doi.org/10.2144/03342mt01
    64 schema:datePublished 2011-08
    65 schema:datePublishedReg 2011-08-01
    66 schema:description The adult stem cell marker Lgr5 and its relative Lgr4 are often co-expressed in Wnt-driven proliferative compartments. We find that conditional deletion of both genes in the mouse gut impairs Wnt target gene expression and results in the rapid demise of intestinal crypts, thus phenocopying Wnt pathway inhibition. Mass spectrometry demonstrates that Lgr4 and Lgr5 associate with the Frizzled/Lrp Wnt receptor complex. Each of the four R-spondins, secreted Wnt pathway agonists, can bind to Lgr4, -5 and -6. In HEK293 cells, RSPO1 enhances canonical WNT signals initiated by WNT3A. Removal of LGR4 does not affect WNT3A signalling, but abrogates the RSPO1-mediated signal enhancement, a phenomenon rescued by re-expression of LGR4, -5 or -6. Genetic deletion of Lgr4/5 in mouse intestinal crypt cultures phenocopies withdrawal of Rspo1 and can be rescued by Wnt pathway activation. Lgr5 homologues are facultative Wnt receptor components that mediate Wnt signal enhancement by soluble R-spondin proteins. These results will guide future studies towards the application of R-spondins for regenerative purposes of tissues expressing Lgr5 homologues.
    67 schema:genre research_article
    68 schema:inLanguage en
    69 schema:isAccessibleForFree false
    70 schema:isPartOf N2f0b485d9ea54dee89e3f1f7fed341c5
    71 Nd399fd3a797b41239ceb132975282ed5
    72 sg:journal.1018957
    73 schema:name Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling
    74 schema:pagination 293
    75 schema:productId N28db8b98e22f4eed9c958d6db245bc34
    76 N2e5ee4459c9348d09bb53e6513292cd4
    77 N4891b5e2d5f54beb814d9b3993b5dbe3
    78 N6a2e4c8295114f7d8abe3c7d7d13aae6
    79 N7555dfdbef8b4a14a46b4f25effb7c68
    80 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053455836
    81 https://doi.org/10.1038/nature10337
    82 schema:sdDatePublished 2019-04-10T16:30
    83 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    84 schema:sdPublisher N2fc11a91bc6f474ab58d63be1d026691
    85 schema:url https://www.nature.com/articles/nature10337
    86 sgo:license sg:explorer/license/
    87 sgo:sdDataset articles
    88 rdf:type schema:ScholarlyArticle
    89 N0696e7aa77c94cc188f6d5ae3b542aef rdf:first sg:person.01313245325.38
    90 rdf:rest Nfa992ece807149b9b0d4b854b1444f8d
    91 N06d507ff28b841c68dc282edee3165fd rdf:first sg:person.0750240753.37
    92 rdf:rest Ndd0fea9310e74be980883f68f34697f8
    93 N0b1664d7e1a7478ebea4004652e5038d schema:name Hubrecht Institute and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands
    94 rdf:type schema:Organization
    95 N104483b5fccd4f4d81d9dc1a176213e5 rdf:first sg:person.01151605507.23
    96 rdf:rest Nac7d662ddc4b4d04abd9ed5afcfd4ed5
    97 N138f437fc84d4bc1a14195f03fe66d12 schema:name Hubrecht Institute and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands
    98 rdf:type schema:Organization
    99 N1ab492614c564633924640e69ad12690 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    100 schema:name Receptors, G-Protein-Coupled
    101 rdf:type schema:DefinedTerm
    102 N24a4692a23ea4e91ac8fa85d2976c0c7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    103 schema:name Animals
    104 rdf:type schema:DefinedTerm
    105 N26e7c6934038401d8f0d4fe1f8336e1f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    106 schema:name Gene Deletion
    107 rdf:type schema:DefinedTerm
    108 N28db8b98e22f4eed9c958d6db245bc34 schema:name dimensions_id
    109 schema:value pub.1053455836
    110 rdf:type schema:PropertyValue
    111 N2b2ba15bafb24b528fa014c17dd7ace1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    112 schema:name Wnt3 Protein
    113 rdf:type schema:DefinedTerm
    114 N2d74d367311d4601a72080c3b6ac8e14 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    115 schema:name Humans
    116 rdf:type schema:DefinedTerm
    117 N2e5ee4459c9348d09bb53e6513292cd4 schema:name nlm_unique_id
    118 schema:value 0410462
    119 rdf:type schema:PropertyValue
    120 N2f0b485d9ea54dee89e3f1f7fed341c5 schema:volumeNumber 476
    121 rdf:type schema:PublicationVolume
    122 N2fc11a91bc6f474ab58d63be1d026691 schema:name Springer Nature - SN SciGraph project
    123 rdf:type schema:Organization
    124 N31ddc3601a4745bf9920d1464c37c3d1 rdf:first sg:person.0666067727.95
    125 rdf:rest Nf6a948cdf57f4659a458cdaf67d7bcee
    126 N379f2954543a4b0493caa43c5060bc9f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name Protein Structure, Tertiary
    128 rdf:type schema:DefinedTerm
    129 N38789e6907ca49b2965b1b0cd48fd2cd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    130 schema:name Signal Transduction
    131 rdf:type schema:DefinedTerm
    132 N4891b5e2d5f54beb814d9b3993b5dbe3 schema:name pubmed_id
    133 schema:value 21727895
    134 rdf:type schema:PropertyValue
    135 N4b7788da699e42199d49e29de82f0a28 rdf:first sg:person.01273351443.89
    136 rdf:rest N5b1c3e6c29a745068a3c2f339196e08a
    137 N54dd04d5a72a4bd78e4d68428c10e97a rdf:first sg:person.01307053755.26
    138 rdf:rest N0696e7aa77c94cc188f6d5ae3b542aef
    139 N5b1c3e6c29a745068a3c2f339196e08a rdf:first sg:person.01132006272.03
    140 rdf:rest N06d507ff28b841c68dc282edee3165fd
    141 N5b4ceb134f9947e2bbc22c9221323204 rdf:first sg:person.01064467353.67
    142 rdf:rest N31ddc3601a4745bf9920d1464c37c3d1
    143 N60b98b29aebd4f7fa2ce9b621bdc8a29 rdf:first sg:person.012054747172.33
    144 rdf:rest Ncec0746d78224c58a8f4ae86f47effa3
    145 N679e45205bf742d79e284a6eceb08515 rdf:first sg:person.01213722537.34
    146 rdf:rest N54dd04d5a72a4bd78e4d68428c10e97a
    147 N6a2e4c8295114f7d8abe3c7d7d13aae6 schema:name readcube_id
    148 schema:value fe9de5c111a1cf3076e8fe425b2dc5c3def58db135207348b2f7c594f28bc3fe
    149 rdf:type schema:PropertyValue
    150 N6c07a1d7578d481a947603598f4d1d3c schema:name Hubrecht Institute and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands
    151 rdf:type schema:Organization
    152 N6e0e2ea51a904535ae306b7833d4acab schema:name Hubrecht Institute and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands
    153 rdf:type schema:Organization
    154 N70602f03eb9040a884649e2c66bbe7e1 schema:name Hubrecht Institute and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands
    155 rdf:type schema:Organization
    156 N7555dfdbef8b4a14a46b4f25effb7c68 schema:name doi
    157 schema:value 10.1038/nature10337
    158 rdf:type schema:PropertyValue
    159 N7ed174d666e64bb7ab8b814f550862fd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    160 schema:name Adult Stem Cells
    161 rdf:type schema:DefinedTerm
    162 N80be3f01a8ee47f8a4ba0a9b7fb50f30 schema:name Antoni van Leeuwenhoek Hospital Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    163 rdf:type schema:Organization
    164 N833e0485eab34a6dbff2dfe95abf6491 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    165 schema:name Epithelial Cells
    166 rdf:type schema:DefinedTerm
    167 N87a320c08ea74b14bfe84a0c44149d3f rdf:first sg:person.07502404177.84
    168 rdf:rest Nc7e43e8a8a4547638c0ed0b18294c774
    169 N98dd24c35fcf44078457cbfcf150f256 schema:name Hubrecht Institute and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands
    170 rdf:type schema:Organization
    171 Na31720df68a04c1e9a615bf718f84cc8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    172 schema:name Regeneration
    173 rdf:type schema:DefinedTerm
    174 Naa6143a80006466d80d2fcd5783c2818 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    175 schema:name Wnt Proteins
    176 rdf:type schema:DefinedTerm
    177 Nab3e5f9906a64082886a5371c769f088 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    178 schema:name Wnt3A Protein
    179 rdf:type schema:DefinedTerm
    180 Nabffae3999f944cea1cd6fa4cf5c685a schema:name Hubrecht Institute and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands
    181 rdf:type schema:Organization
    182 Nac7d662ddc4b4d04abd9ed5afcfd4ed5 rdf:first sg:person.01237336020.34
    183 rdf:rest N87a320c08ea74b14bfe84a0c44149d3f
    184 Nae411acda6254586a03f37b693976cb3 schema:name Hubrecht Institute and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands
    185 rdf:type schema:Organization
    186 Nb0a7dd555cc748deb6c28e461e4209f3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    187 schema:name Cells, Cultured
    188 rdf:type schema:DefinedTerm
    189 Nb0de664971e34fe59bc68ccca5db28cc schema:name Hubrecht Institute and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands
    190 rdf:type schema:Organization
    191 Nbcb924303e194fdc899656112f4dcc37 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    192 schema:name Protein Binding
    193 rdf:type schema:DefinedTerm
    194 Nc70b21eb598c485ebb58a399393ad30a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    195 schema:name Mice
    196 rdf:type schema:DefinedTerm
    197 Nc7e43e8a8a4547638c0ed0b18294c774 rdf:first sg:person.0662243670.45
    198 rdf:rest rdf:nil
    199 Nca0e8340bf1440438047bd170dd6328c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    200 schema:name Frizzled Receptors
    201 rdf:type schema:DefinedTerm
    202 Ncec0746d78224c58a8f4ae86f47effa3 rdf:first sg:person.0677136307.17
    203 rdf:rest Ndfaf08fc4eb644b599ed8a7a4ec45660
    204 Nd399fd3a797b41239ceb132975282ed5 schema:issueNumber 7360
    205 rdf:type schema:PublicationIssue
    206 Ndd0fea9310e74be980883f68f34697f8 rdf:first sg:person.0643614125.13
    207 rdf:rest N5b4ceb134f9947e2bbc22c9221323204
    208 Ndfaf08fc4eb644b599ed8a7a4ec45660 rdf:first sg:person.0616060543.03
    209 rdf:rest N4b7788da699e42199d49e29de82f0a28
    210 Ne970c10778ee4e15ba004ac6d5328891 schema:name Antoni van Leeuwenhoek Hospital Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    211 rdf:type schema:Organization
    212 Nf021819a8e4f474c8e9315ae80deea20 rdf:first sg:person.0735472007.92
    213 rdf:rest N104483b5fccd4f4d81d9dc1a176213e5
    214 Nf3dedc2a832a4cebbd4d1822ac9faaa4 schema:name Hubrecht Institute and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands
    215 rdf:type schema:Organization
    216 Nf602a83b31ea4eb1aee1dbdcbd20c4fd schema:name Hubrecht Institute and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands
    217 rdf:type schema:Organization
    218 Nf6a948cdf57f4659a458cdaf67d7bcee rdf:first sg:person.015536155047.43
    219 rdf:rest Nf021819a8e4f474c8e9315ae80deea20
    220 Nf8f2c3455ede4029988170c6c1c3fbce schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    221 schema:name HEK293 Cells
    222 rdf:type schema:DefinedTerm
    223 Nfa992ece807149b9b0d4b854b1444f8d rdf:first sg:person.01352156370.23
    224 rdf:rest N60b98b29aebd4f7fa2ce9b621bdc8a29
    225 Nfcaec8869c1c4b4ea13be99f6d18b5db schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    226 schema:name Thrombospondins
    227 rdf:type schema:DefinedTerm
    228 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    229 schema:name Biological Sciences
    230 rdf:type schema:DefinedTerm
    231 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    232 schema:name Biochemistry and Cell Biology
    233 rdf:type schema:DefinedTerm
    234 sg:grant.6047570 http://pending.schema.org/fundedItem sg:pub.10.1038/nature10337
    235 rdf:type schema:MonetaryGrant
    236 sg:grant.6058394 http://pending.schema.org/fundedItem sg:pub.10.1038/nature10337
    237 rdf:type schema:MonetaryGrant
    238 sg:journal.1018957 schema:issn 0090-0028
    239 1476-4687
    240 schema:name Nature
    241 rdf:type schema:Periodical
    242 sg:person.01064467353.67 schema:affiliation N70602f03eb9040a884649e2c66bbe7e1
    243 schema:familyName van Es
    244 schema:givenName J.
    245 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01064467353.67
    246 rdf:type schema:Person
    247 sg:person.01132006272.03 schema:affiliation Ne970c10778ee4e15ba004ac6d5328891
    248 schema:familyName Peters
    249 schema:givenName Peter J.
    250 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132006272.03
    251 rdf:type schema:Person
    252 sg:person.01151605507.23 schema:affiliation https://www.grid.ac/institutes/grid.69566.3a
    253 schema:familyName Nishimori
    254 schema:givenName Katsuhiko
    255 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01151605507.23
    256 rdf:type schema:Person
    257 sg:person.012054747172.33 schema:affiliation Nf602a83b31ea4eb1aee1dbdcbd20c4fd
    258 schema:familyName Li
    259 schema:givenName Vivian S. W.
    260 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012054747172.33
    261 rdf:type schema:Person
    262 sg:person.01213722537.34 schema:affiliation Nae411acda6254586a03f37b693976cb3
    263 schema:familyName de Lau
    264 schema:givenName Wim
    265 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01213722537.34
    266 rdf:type schema:Person
    267 sg:person.01237336020.34 schema:affiliation https://www.grid.ac/institutes/grid.5477.1
    268 schema:familyName Mohammed
    269 schema:givenName Shabaz
    270 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01237336020.34
    271 rdf:type schema:Person
    272 sg:person.01273351443.89 schema:affiliation N0b1664d7e1a7478ebea4004652e5038d
    273 schema:familyName Haegebarth
    274 schema:givenName Andrea
    275 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01273351443.89
    276 rdf:type schema:Person
    277 sg:person.01307053755.26 schema:affiliation N6c07a1d7578d481a947603598f4d1d3c
    278 schema:familyName Barker
    279 schema:givenName Nick
    280 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307053755.26
    281 rdf:type schema:Person
    282 sg:person.01313245325.38 schema:affiliation https://www.grid.ac/institutes/grid.5477.1
    283 schema:familyName Low
    284 schema:givenName Teck Y.
    285 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313245325.38
    286 rdf:type schema:Person
    287 sg:person.01352156370.23 schema:affiliation N6e0e2ea51a904535ae306b7833d4acab
    288 schema:familyName Koo
    289 schema:givenName Bon-Kyoung
    290 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01352156370.23
    291 rdf:type schema:Person
    292 sg:person.015536155047.43 schema:affiliation https://www.grid.ac/institutes/grid.6214.1
    293 schema:familyName Schasfoort
    294 schema:givenName Richard B. M.
    295 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015536155047.43
    296 rdf:type schema:Person
    297 sg:person.0616060543.03 schema:affiliation N80be3f01a8ee47f8a4ba0a9b7fb50f30
    298 schema:familyName Kujala
    299 schema:givenName Pekka
    300 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0616060543.03
    301 rdf:type schema:Person
    302 sg:person.0643614125.13 schema:affiliation N98dd24c35fcf44078457cbfcf150f256
    303 schema:familyName Stange
    304 schema:givenName D. E.
    305 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0643614125.13
    306 rdf:type schema:Person
    307 sg:person.0662243670.45 schema:affiliation Nabffae3999f944cea1cd6fa4cf5c685a
    308 schema:familyName Clevers
    309 schema:givenName Hans
    310 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0662243670.45
    311 rdf:type schema:Person
    312 sg:person.0666067727.95 schema:affiliation N138f437fc84d4bc1a14195f03fe66d12
    313 schema:familyName Guardavaccaro
    314 schema:givenName Daniele
    315 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0666067727.95
    316 rdf:type schema:Person
    317 sg:person.0677136307.17 schema:affiliation Nf3dedc2a832a4cebbd4d1822ac9faaa4
    318 schema:familyName Teunissen
    319 schema:givenName Hans
    320 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677136307.17
    321 rdf:type schema:Person
    322 sg:person.0735472007.92 schema:affiliation https://www.grid.ac/institutes/grid.69566.3a
    323 schema:familyName Mohri
    324 schema:givenName Yasuaki
    325 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0735472007.92
    326 rdf:type schema:Person
    327 sg:person.07502404177.84 schema:affiliation https://www.grid.ac/institutes/grid.5477.1
    328 schema:familyName Heck
    329 schema:givenName Albert J. R.
    330 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07502404177.84
    331 rdf:type schema:Person
    332 sg:person.0750240753.37 schema:affiliation Nb0de664971e34fe59bc68ccca5db28cc
    333 schema:familyName van de Wetering
    334 schema:givenName Marc
    335 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0750240753.37
    336 rdf:type schema:Person
    337 sg:pub.10.1007/s00418-005-0002-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044478492
    338 https://doi.org/10.1007/s00418-005-0002-3
    339 rdf:type schema:CreativeWork
    340 sg:pub.10.1038/1270 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002695839
    341 https://doi.org/10.1038/1270
    342 rdf:type schema:CreativeWork
    343 sg:pub.10.1038/34848 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051110109
    344 https://doi.org/10.1038/34848
    345 rdf:type schema:CreativeWork
    346 sg:pub.10.1038/nature03659 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046707660
    347 https://doi.org/10.1038/nature03659
    348 rdf:type schema:CreativeWork
    349 sg:pub.10.1038/nature05674 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002848303
    350 https://doi.org/10.1038/nature05674
    351 rdf:type schema:CreativeWork
    352 sg:pub.10.1038/nature06196 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050225537
    353 https://doi.org/10.1038/nature06196
    354 rdf:type schema:CreativeWork
    355 sg:pub.10.1038/nature07935 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024082069
    356 https://doi.org/10.1038/nature07935
    357 rdf:type schema:CreativeWork
    358 sg:pub.10.1038/nature09637 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030684957
    359 https://doi.org/10.1038/nature09637
    360 rdf:type schema:CreativeWork
    361 sg:pub.10.1038/nbt.1511 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049469421
    362 https://doi.org/10.1038/nbt.1511
    363 rdf:type schema:CreativeWork
    364 sg:pub.10.1038/ng.239 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023194186
    365 https://doi.org/10.1038/ng.239
    366 rdf:type schema:CreativeWork
    367 https://app.dimensions.ai/details/publication/pub.1082831306 schema:CreativeWork
    368 https://doi.org/10.1002/dvdy.20446 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031742698
    369 rdf:type schema:CreativeWork
    370 https://doi.org/10.1016/j.cell.2009.01.031 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003917776
    371 rdf:type schema:CreativeWork
    372 https://doi.org/10.1016/j.devcel.2004.07.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024994615
    373 rdf:type schema:CreativeWork
    374 https://doi.org/10.1016/j.devcel.2011.01.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038585487
    375 rdf:type schema:CreativeWork
    376 https://doi.org/10.1016/j.febslet.2007.08.064 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016812609
    377 rdf:type schema:CreativeWork
    378 https://doi.org/10.1016/j.stem.2009.11.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039362335
    379 rdf:type schema:CreativeWork
    380 https://doi.org/10.1016/j.ydbio.2009.04.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013884866
    381 rdf:type schema:CreativeWork
    382 https://doi.org/10.1016/s0076-6879(03)70037-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035247640
    383 rdf:type schema:CreativeWork
    384 https://doi.org/10.1021/ac950914h schema:sameAs https://app.dimensions.ai/details/publication/pub.1055072376
    385 rdf:type schema:CreativeWork
    386 https://doi.org/10.1021/pr1011729 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039087538
    387 rdf:type schema:CreativeWork
    388 https://doi.org/10.1038/embor.2011.52 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028716824
    389 rdf:type schema:CreativeWork
    390 https://doi.org/10.1053/j.gastro.2007.02.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004526083
    391 rdf:type schema:CreativeWork
    392 https://doi.org/10.1053/j.gastro.2009.05.035 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001779262
    393 rdf:type schema:CreativeWork
    394 https://doi.org/10.1053/j.gastro.2010.03.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025461503
    395 rdf:type schema:CreativeWork
    396 https://doi.org/10.1073/pnas.0506580102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037705714
    397 rdf:type schema:CreativeWork
    398 https://doi.org/10.1073/pnas.0702305104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045150931
    399 rdf:type schema:CreativeWork
    400 https://doi.org/10.1073/pnas.2536800100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050279034
    401 rdf:type schema:CreativeWork
    402 https://doi.org/10.1073/pnas.93.16.8455 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035953957
    403 rdf:type schema:CreativeWork
    404 https://doi.org/10.1074/jbc.m109.078261 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018759398
    405 rdf:type schema:CreativeWork
    406 https://doi.org/10.1074/jbc.m508324200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017083954
    407 rdf:type schema:CreativeWork
    408 https://doi.org/10.1074/jbc.m701927200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007327575
    409 rdf:type schema:CreativeWork
    410 https://doi.org/10.1074/mcp.m800093-mcp200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031511342
    411 rdf:type schema:CreativeWork
    412 https://doi.org/10.1091/mbc.e08-02-0187 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022351840
    413 rdf:type schema:CreativeWork
    414 https://doi.org/10.1101/gad.267103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011480346
    415 rdf:type schema:CreativeWork
    416 https://doi.org/10.1124/mol.105.014183 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031657105
    417 rdf:type schema:CreativeWork
    418 https://doi.org/10.1126/science.1112521 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050697196
    419 rdf:type schema:CreativeWork
    420 https://doi.org/10.1126/science.1184733 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002969630
    421 rdf:type schema:CreativeWork
    422 https://doi.org/10.1126/science.275.5307.1784 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062556119
    423 rdf:type schema:CreativeWork
    424 https://doi.org/10.1128/mcb.00821-06 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040375340
    425 rdf:type schema:CreativeWork
    426 https://doi.org/10.1128/mcb.24.22.9736-9743.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003545217
    427 rdf:type schema:CreativeWork
    428 https://doi.org/10.2144/03342mt01 schema:sameAs https://app.dimensions.ai/details/publication/pub.1075245738
    429 rdf:type schema:CreativeWork
    430 https://www.grid.ac/institutes/grid.5477.1 schema:alternateName Utrecht University
    431 schema:name Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center, Utrecht University, 3584 CH Utrecht, The Netherlands
    432 rdf:type schema:Organization
    433 https://www.grid.ac/institutes/grid.6214.1 schema:alternateName University of Twente
    434 schema:name Medical Cell Biophysics, MIRA institute, University of Twente, 7500 AE Enschede, The Netherlands
    435 rdf:type schema:Organization
    436 https://www.grid.ac/institutes/grid.69566.3a schema:alternateName Tohoku University
    437 schema:name Tohoku University, 981-8555 Sendai, Miyagi, Japan
    438 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...