CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-03

AUTHORS

Elitza Deltcheva, Krzysztof Chylinski, Cynthia M. Sharma, Karine Gonzales, Yanjie Chao, Zaid A. Pirzada, Maria R. Eckert, Jörg Vogel, Emmanuelle Charpentier

ABSTRACT

CRISPR/Cas systems constitute a widespread class of immunity systems that protect bacteria and archaea against phages and plasmids, and commonly use repeat/spacer-derived short crRNAs to silence foreign nucleic acids in a sequence-specific manner. Although the maturation of crRNAs represents a key event in CRISPR activation, the responsible endoribonucleases (CasE, Cas6, Csy4) are missing in many CRISPR/Cas subtypes. Here, differential RNA sequencing of the human pathogen Streptococcus pyogenes uncovered tracrRNA, a trans-encoded small RNA with 24-nucleotide complementarity to the repeat regions of crRNA precursor transcripts. We show that tracrRNA directs the maturation of crRNAs by the activities of the widely conserved endogenous RNase III and the CRISPR-associated Csn1 protein; all these components are essential to protect S. pyogenes against prophage-derived DNA. Our study reveals a novel pathway of small guide RNA maturation and the first example of a host factor (RNase III) required for bacterial RNA-mediated immunity against invaders. More... »

PAGES

602

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  • Journal

    TITLE

    Nature

    ISSUE

    7340

    VOLUME

    471

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nature09886

    DOI

    http://dx.doi.org/10.1038/nature09886

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1030591890

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/21455174


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    386 https://www.grid.ac/institutes/grid.8379.5 schema:alternateName University of Würzburg
    387 schema:name RNA Biology Group, Institute for Molecular Infection Biology, University of Würzburg, D-97080 Würzburg, Germany
    388 ZINF Research Center for Infectious Diseases, University of Würzburg, D-97080 Würzburg, Germany
    389 rdf:type schema:Organization
     




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