Structural basis of abscisic acid signalling View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-12

AUTHORS

Ken-ichi Miyazono, Takuya Miyakawa, Yoriko Sawano, Keiko Kubota, Hee-Jin Kang, Atsuko Asano, Yumiko Miyauchi, Mihoko Takahashi, Yuehua Zhi, Yasunari Fujita, Takuya Yoshida, Ken-Suke Kodaira, Kazuko Yamaguchi-Shinozaki, Masaru Tanokura

ABSTRACT

The phytohormone abscisic acid (ABA) mediates the adaptation of plants to environmental stresses such as drought and regulates developmental signals such as seed maturation. Within plants, the PYR/PYL/RCAR family of START proteins receives ABA to inhibit the phosphatase activity of the group-A protein phosphatases 2C (PP2Cs), which are major negative regulators in ABA signalling. Here we present the crystal structures of the ABA receptor PYL1 bound with (+)-ABA, and the complex formed by the further binding of (+)-ABA-bound PYL1 with the PP2C protein ABI1. PYL1 binds (+)-ABA using the START-protein-specific ligand-binding site, thereby forming a hydrophobic pocket on the surface of the closed lid. (+)-ABA-bound PYL1 tightly interacts with a PP2C domain of ABI1 by using the hydrophobic pocket to cover the active site of ABI1 like a plug. Our results reveal the structural basis of the mechanism of (+)-ABA-dependent inhibition of ABI1 by PYL1 in ABA signalling. More... »

PAGES

609

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nature08583

DOI

http://dx.doi.org/10.1038/nature08583

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1021371534

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19855379


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Abscisic Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Arabidopsis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Arabidopsis Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Binding Sites", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Binding", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Structure, Tertiary", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Signal Transduction", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Tokyo", 
          "id": "https://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Miyazono", 
        "givenName": "Ken-ichi", 
        "id": "sg:person.0742444277.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0742444277.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Tokyo", 
          "id": "https://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Miyakawa", 
        "givenName": "Takuya", 
        "id": "sg:person.0750716211.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0750716211.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Tokyo", 
          "id": "https://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sawano", 
        "givenName": "Yoriko", 
        "id": "sg:person.01114577506.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01114577506.28"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Tokyo", 
          "id": "https://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kubota", 
        "givenName": "Keiko", 
        "id": "sg:person.01024031101.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01024031101.41"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Tokyo", 
          "id": "https://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kang", 
        "givenName": "Hee-Jin", 
        "id": "sg:person.01334271626.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01334271626.41"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Tokyo", 
          "id": "https://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Asano", 
        "givenName": "Atsuko", 
        "id": "sg:person.01225217134.96", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01225217134.96"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Tokyo", 
          "id": "https://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Miyauchi", 
        "givenName": "Yumiko", 
        "id": "sg:person.01036554437.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01036554437.18"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Tokyo", 
          "id": "https://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Takahashi", 
        "givenName": "Mihoko", 
        "id": "sg:person.01157103734.63", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01157103734.63"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Tokyo", 
          "id": "https://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhi", 
        "givenName": "Yuehua", 
        "id": "sg:person.0727013105.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0727013105.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Japan International Research Center for Agricultural Sciences", 
          "id": "https://www.grid.ac/institutes/grid.452611.5", 
          "name": [
            "Biological Resources Division, Japan International Research Center for Agricultural Sciences, Ibaraki 305-8686, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fujita", 
        "givenName": "Yasunari", 
        "id": "sg:person.01076661555.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076661555.55"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Tokyo", 
          "id": "https://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Biological Resources Division, Japan International Research Center for Agricultural Sciences, Ibaraki 305-8686, Japan", 
            "Laboratory of Plant Molecular Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yoshida", 
        "givenName": "Takuya", 
        "id": "sg:person.0604543536.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0604543536.27"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Tokyo", 
          "id": "https://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Biological Resources Division, Japan International Research Center for Agricultural Sciences, Ibaraki 305-8686, Japan", 
            "Laboratory of Plant Molecular Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kodaira", 
        "givenName": "Ken-Suke", 
        "id": "sg:person.0631513414.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0631513414.57"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Tokyo", 
          "id": "https://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Biological Resources Division, Japan International Research Center for Agricultural Sciences, Ibaraki 305-8686, Japan", 
            "Laboratory of Plant Molecular Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yamaguchi-Shinozaki", 
        "givenName": "Kazuko", 
        "id": "sg:person.01201751012.72", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01201751012.72"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Tokyo", 
          "id": "https://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tanokura", 
        "givenName": "Masaru", 
        "id": "sg:person.01130231021.49", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01130231021.49"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1126/science.1173041", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001347012"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00197809", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001469691", 
          "https://doi.org/10.1007/bf00197809"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00197809", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001469691", 
          "https://doi.org/10.1007/bf00197809"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0903144106", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001786029"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1172408", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002554612"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/prot.10286", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002914406"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jsbi.1999.4094", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005545483"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tplants.2007.06.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005792124"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/pcp/pcf188", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006712983"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444902018036", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015609779"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444996012255", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020405374"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444902011678", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021532775"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-3054.1984.tb06343.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022864617"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444903017694", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030505707"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(71)90324-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032169433"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.019943", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033952129"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m405259200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038455205"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.106.048538", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038995574"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(68)90205-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039875707"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.007906", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045021619"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0505667103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045711607"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/pcp/pcp083", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047784337"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0021889893005588", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053164838"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.324_1012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062601286"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.7910981", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062650617"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.8197457", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062653088"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076880564", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/oxfordjournals.jbchem.a134653", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1081924567"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082816658", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1996.tb01071.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083035063"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2009-12", 
    "datePublishedReg": "2009-12-01", 
    "description": "The phytohormone abscisic acid (ABA) mediates the adaptation of plants to environmental stresses such as drought and regulates developmental signals such as seed maturation. Within plants, the PYR/PYL/RCAR family of START proteins receives ABA to inhibit the phosphatase activity of the group-A protein phosphatases 2C (PP2Cs), which are major negative regulators in ABA signalling. Here we present the crystal structures of the ABA receptor PYL1 bound with (+)-ABA, and the complex formed by the further binding of (+)-ABA-bound PYL1 with the PP2C protein ABI1. PYL1 binds (+)-ABA using the START-protein-specific ligand-binding site, thereby forming a hydrophobic pocket on the surface of the closed lid. (+)-ABA-bound PYL1 tightly interacts with a PP2C domain of ABI1 by using the hydrophobic pocket to cover the active site of ABI1 like a plug. Our results reveal the structural basis of the mechanism of (+)-ABA-dependent inhibition of ABI1 by PYL1 in ABA signalling.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nature08583", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7273", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "462"
      }
    ], 
    "name": "Structural basis of abscisic acid signalling", 
    "pagination": "609", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "65d2efd942c1e15214ef259f018ce2a91d9b5dd206ef3b029c28f24f3249d899"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "19855379"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nature08583"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1021371534"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nature08583", 
      "https://app.dimensions.ai/details/publication/pub.1021371534"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T09:31", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000346_0000000346/records_99803_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/nature08583"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature08583'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature08583'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature08583'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature08583'


 

This table displays all metadata directly associated to this object as RDF triples.

287 TRIPLES      21 PREDICATES      67 URIs      30 LITERALS      18 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nature08583 schema:about N219c5eb939d14fcba7929feaf3e875c8
2 N2afe4b7665bd428a9d12f558dc308f3b
3 N55e16ebf59f944bc83eef65f22ba0d75
4 N9b47515424d1415e8400d139f5248fa4
5 Nbc56f5cbac7f415885aae840c31200ab
6 Nc0383a43e2984b11b1f76531b03bb18b
7 Nc1e39f0216344b708a799d6fda5226cd
8 Ndcb8aaef04144a739fc1622ecbda4ebe
9 Ne359dc45ebcf448e830d3f7d52e92e4a
10 anzsrc-for:06
11 anzsrc-for:0601
12 schema:author N14458a4b2c584746b8b6fb437cdb96e0
13 schema:citation sg:pub.10.1007/bf00197809
14 https://app.dimensions.ai/details/publication/pub.1076880564
15 https://app.dimensions.ai/details/publication/pub.1082816658
16 https://doi.org/10.1002/j.1460-2075.1996.tb01071.x
17 https://doi.org/10.1002/prot.10286
18 https://doi.org/10.1006/jsbi.1999.4094
19 https://doi.org/10.1016/0022-2836(68)90205-2
20 https://doi.org/10.1016/0022-2836(71)90324-x
21 https://doi.org/10.1016/j.tplants.2007.06.013
22 https://doi.org/10.1073/pnas.0505667103
23 https://doi.org/10.1073/pnas.0903144106
24 https://doi.org/10.1074/jbc.m405259200
25 https://doi.org/10.1093/oxfordjournals.jbchem.a134653
26 https://doi.org/10.1093/pcp/pcf188
27 https://doi.org/10.1093/pcp/pcp083
28 https://doi.org/10.1105/tpc.007906
29 https://doi.org/10.1105/tpc.019943
30 https://doi.org/10.1105/tpc.106.048538
31 https://doi.org/10.1107/s0021889893005588
32 https://doi.org/10.1107/s0907444902011678
33 https://doi.org/10.1107/s0907444902018036
34 https://doi.org/10.1107/s0907444903017694
35 https://doi.org/10.1107/s0907444996012255
36 https://doi.org/10.1111/j.1399-3054.1984.tb06343.x
37 https://doi.org/10.1126/science.1172408
38 https://doi.org/10.1126/science.1173041
39 https://doi.org/10.1126/science.324_1012
40 https://doi.org/10.1126/science.7910981
41 https://doi.org/10.1126/science.8197457
42 schema:datePublished 2009-12
43 schema:datePublishedReg 2009-12-01
44 schema:description The phytohormone abscisic acid (ABA) mediates the adaptation of plants to environmental stresses such as drought and regulates developmental signals such as seed maturation. Within plants, the PYR/PYL/RCAR family of START proteins receives ABA to inhibit the phosphatase activity of the group-A protein phosphatases 2C (PP2Cs), which are major negative regulators in ABA signalling. Here we present the crystal structures of the ABA receptor PYL1 bound with (+)-ABA, and the complex formed by the further binding of (+)-ABA-bound PYL1 with the PP2C protein ABI1. PYL1 binds (+)-ABA using the START-protein-specific ligand-binding site, thereby forming a hydrophobic pocket on the surface of the closed lid. (+)-ABA-bound PYL1 tightly interacts with a PP2C domain of ABI1 by using the hydrophobic pocket to cover the active site of ABI1 like a plug. Our results reveal the structural basis of the mechanism of (+)-ABA-dependent inhibition of ABI1 by PYL1 in ABA signalling.
45 schema:genre research_article
46 schema:inLanguage en
47 schema:isAccessibleForFree false
48 schema:isPartOf N2493bd1db5084ec68b2462bf2b29686e
49 N877045da556b4b6b883510f9e4a89fa6
50 sg:journal.1018957
51 schema:name Structural basis of abscisic acid signalling
52 schema:pagination 609
53 schema:productId N092a98f7db914ea88afacaa5a26baee9
54 N15f2c054c94640af84a3b3f1ec0de9ed
55 N206d1b314d0e49828f8f38c003f42053
56 Na5b6df26a0f044ac845bb8d6f5077022
57 Nea47f3d6d96e48b7b9edf9632be8ced7
58 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021371534
59 https://doi.org/10.1038/nature08583
60 schema:sdDatePublished 2019-04-11T09:31
61 schema:sdLicense https://scigraph.springernature.com/explorer/license/
62 schema:sdPublisher Na87cbbdaf4a74b31b2cb1600f3d54b99
63 schema:url https://www.nature.com/articles/nature08583
64 sgo:license sg:explorer/license/
65 sgo:sdDataset articles
66 rdf:type schema:ScholarlyArticle
67 N092a98f7db914ea88afacaa5a26baee9 schema:name readcube_id
68 schema:value 65d2efd942c1e15214ef259f018ce2a91d9b5dd206ef3b029c28f24f3249d899
69 rdf:type schema:PropertyValue
70 N10171f78d992415097f14e2c72e21ce5 rdf:first sg:person.01024031101.41
71 rdf:rest N65985024e52547e6a1f53d07ea3e239e
72 N14458a4b2c584746b8b6fb437cdb96e0 rdf:first sg:person.0742444277.09
73 rdf:rest Ne9cb96a6d3e84e4e820a68c96e04dcf5
74 N15f2c054c94640af84a3b3f1ec0de9ed schema:name pubmed_id
75 schema:value 19855379
76 rdf:type schema:PropertyValue
77 N206d1b314d0e49828f8f38c003f42053 schema:name doi
78 schema:value 10.1038/nature08583
79 rdf:type schema:PropertyValue
80 N219c5eb939d14fcba7929feaf3e875c8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name Arabidopsis Proteins
82 rdf:type schema:DefinedTerm
83 N2493bd1db5084ec68b2462bf2b29686e schema:volumeNumber 462
84 rdf:type schema:PublicationVolume
85 N2afe4b7665bd428a9d12f558dc308f3b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Signal Transduction
87 rdf:type schema:DefinedTerm
88 N55e16ebf59f944bc83eef65f22ba0d75 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Arabidopsis
90 rdf:type schema:DefinedTerm
91 N5e5cb672952a4343b668e4cc3640754e rdf:first sg:person.01114577506.28
92 rdf:rest N10171f78d992415097f14e2c72e21ce5
93 N65985024e52547e6a1f53d07ea3e239e rdf:first sg:person.01334271626.41
94 rdf:rest Nb7a03ae04f7449eea02fedfcc91f6e5c
95 N877045da556b4b6b883510f9e4a89fa6 schema:issueNumber 7273
96 rdf:type schema:PublicationIssue
97 N88c544e173a846b9b6ec0e6db99c4037 rdf:first sg:person.01201751012.72
98 rdf:rest Nbf12dc4e743642408a2f21ea5f599b05
99 N9b47515424d1415e8400d139f5248fa4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Protein Structure, Tertiary
101 rdf:type schema:DefinedTerm
102 Na5b6df26a0f044ac845bb8d6f5077022 schema:name nlm_unique_id
103 schema:value 0410462
104 rdf:type schema:PropertyValue
105 Na87cbbdaf4a74b31b2cb1600f3d54b99 schema:name Springer Nature - SN SciGraph project
106 rdf:type schema:Organization
107 Na8f4c098dfef4477adadf35ae8185c01 rdf:first sg:person.0727013105.11
108 rdf:rest Nfd9ff33ce2404fa2bf813d4b37c32fa1
109 Nb7a03ae04f7449eea02fedfcc91f6e5c rdf:first sg:person.01225217134.96
110 rdf:rest Nf80e54535a614c74a8116fbc60176fff
111 Nbad3cb2ada5e43a8a6d4cef799a0e1af rdf:first sg:person.0604543536.27
112 rdf:rest Nca0d18a183b94c63b32df9e7ba894f28
113 Nbb24dcbab67e4566834543180d190b9e rdf:first sg:person.01157103734.63
114 rdf:rest Na8f4c098dfef4477adadf35ae8185c01
115 Nbc56f5cbac7f415885aae840c31200ab schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Models, Molecular
117 rdf:type schema:DefinedTerm
118 Nbf12dc4e743642408a2f21ea5f599b05 rdf:first sg:person.01130231021.49
119 rdf:rest rdf:nil
120 Nc0383a43e2984b11b1f76531b03bb18b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Recombinant Proteins
122 rdf:type schema:DefinedTerm
123 Nc1e39f0216344b708a799d6fda5226cd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Abscisic Acid
125 rdf:type schema:DefinedTerm
126 Nca0d18a183b94c63b32df9e7ba894f28 rdf:first sg:person.0631513414.57
127 rdf:rest N88c544e173a846b9b6ec0e6db99c4037
128 Ndcb8aaef04144a739fc1622ecbda4ebe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Binding Sites
130 rdf:type schema:DefinedTerm
131 Ne359dc45ebcf448e830d3f7d52e92e4a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Protein Binding
133 rdf:type schema:DefinedTerm
134 Ne9cb96a6d3e84e4e820a68c96e04dcf5 rdf:first sg:person.0750716211.20
135 rdf:rest N5e5cb672952a4343b668e4cc3640754e
136 Nea47f3d6d96e48b7b9edf9632be8ced7 schema:name dimensions_id
137 schema:value pub.1021371534
138 rdf:type schema:PropertyValue
139 Nf80e54535a614c74a8116fbc60176fff rdf:first sg:person.01036554437.18
140 rdf:rest Nbb24dcbab67e4566834543180d190b9e
141 Nfd9ff33ce2404fa2bf813d4b37c32fa1 rdf:first sg:person.01076661555.55
142 rdf:rest Nbad3cb2ada5e43a8a6d4cef799a0e1af
143 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
144 schema:name Biological Sciences
145 rdf:type schema:DefinedTerm
146 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
147 schema:name Biochemistry and Cell Biology
148 rdf:type schema:DefinedTerm
149 sg:journal.1018957 schema:issn 0090-0028
150 1476-4687
151 schema:name Nature
152 rdf:type schema:Periodical
153 sg:person.01024031101.41 schema:affiliation https://www.grid.ac/institutes/grid.26999.3d
154 schema:familyName Kubota
155 schema:givenName Keiko
156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01024031101.41
157 rdf:type schema:Person
158 sg:person.01036554437.18 schema:affiliation https://www.grid.ac/institutes/grid.26999.3d
159 schema:familyName Miyauchi
160 schema:givenName Yumiko
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01036554437.18
162 rdf:type schema:Person
163 sg:person.01076661555.55 schema:affiliation https://www.grid.ac/institutes/grid.452611.5
164 schema:familyName Fujita
165 schema:givenName Yasunari
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076661555.55
167 rdf:type schema:Person
168 sg:person.01114577506.28 schema:affiliation https://www.grid.ac/institutes/grid.26999.3d
169 schema:familyName Sawano
170 schema:givenName Yoriko
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01114577506.28
172 rdf:type schema:Person
173 sg:person.01130231021.49 schema:affiliation https://www.grid.ac/institutes/grid.26999.3d
174 schema:familyName Tanokura
175 schema:givenName Masaru
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01130231021.49
177 rdf:type schema:Person
178 sg:person.01157103734.63 schema:affiliation https://www.grid.ac/institutes/grid.26999.3d
179 schema:familyName Takahashi
180 schema:givenName Mihoko
181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01157103734.63
182 rdf:type schema:Person
183 sg:person.01201751012.72 schema:affiliation https://www.grid.ac/institutes/grid.26999.3d
184 schema:familyName Yamaguchi-Shinozaki
185 schema:givenName Kazuko
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01201751012.72
187 rdf:type schema:Person
188 sg:person.01225217134.96 schema:affiliation https://www.grid.ac/institutes/grid.26999.3d
189 schema:familyName Asano
190 schema:givenName Atsuko
191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01225217134.96
192 rdf:type schema:Person
193 sg:person.01334271626.41 schema:affiliation https://www.grid.ac/institutes/grid.26999.3d
194 schema:familyName Kang
195 schema:givenName Hee-Jin
196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01334271626.41
197 rdf:type schema:Person
198 sg:person.0604543536.27 schema:affiliation https://www.grid.ac/institutes/grid.26999.3d
199 schema:familyName Yoshida
200 schema:givenName Takuya
201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0604543536.27
202 rdf:type schema:Person
203 sg:person.0631513414.57 schema:affiliation https://www.grid.ac/institutes/grid.26999.3d
204 schema:familyName Kodaira
205 schema:givenName Ken-Suke
206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0631513414.57
207 rdf:type schema:Person
208 sg:person.0727013105.11 schema:affiliation https://www.grid.ac/institutes/grid.26999.3d
209 schema:familyName Zhi
210 schema:givenName Yuehua
211 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0727013105.11
212 rdf:type schema:Person
213 sg:person.0742444277.09 schema:affiliation https://www.grid.ac/institutes/grid.26999.3d
214 schema:familyName Miyazono
215 schema:givenName Ken-ichi
216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0742444277.09
217 rdf:type schema:Person
218 sg:person.0750716211.20 schema:affiliation https://www.grid.ac/institutes/grid.26999.3d
219 schema:familyName Miyakawa
220 schema:givenName Takuya
221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0750716211.20
222 rdf:type schema:Person
223 sg:pub.10.1007/bf00197809 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001469691
224 https://doi.org/10.1007/bf00197809
225 rdf:type schema:CreativeWork
226 https://app.dimensions.ai/details/publication/pub.1076880564 schema:CreativeWork
227 https://app.dimensions.ai/details/publication/pub.1082816658 schema:CreativeWork
228 https://doi.org/10.1002/j.1460-2075.1996.tb01071.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1083035063
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1002/prot.10286 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002914406
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1006/jsbi.1999.4094 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005545483
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1016/0022-2836(68)90205-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039875707
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1016/0022-2836(71)90324-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1032169433
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1016/j.tplants.2007.06.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005792124
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1073/pnas.0505667103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045711607
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1073/pnas.0903144106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001786029
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1074/jbc.m405259200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038455205
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1093/oxfordjournals.jbchem.a134653 schema:sameAs https://app.dimensions.ai/details/publication/pub.1081924567
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1093/pcp/pcf188 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006712983
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1093/pcp/pcp083 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047784337
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1105/tpc.007906 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045021619
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1105/tpc.019943 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033952129
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1105/tpc.106.048538 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038995574
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1107/s0021889893005588 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053164838
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1107/s0907444902011678 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021532775
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1107/s0907444902018036 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015609779
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1107/s0907444903017694 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030505707
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1107/s0907444996012255 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020405374
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1111/j.1399-3054.1984.tb06343.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1022864617
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1126/science.1172408 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002554612
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1126/science.1173041 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001347012
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1126/science.324_1012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062601286
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1126/science.7910981 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062650617
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1126/science.8197457 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062653088
279 rdf:type schema:CreativeWork
280 https://www.grid.ac/institutes/grid.26999.3d schema:alternateName University of Tokyo
281 schema:name Biological Resources Division, Japan International Research Center for Agricultural Sciences, Ibaraki 305-8686, Japan
282 Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
283 Laboratory of Plant Molecular Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
284 rdf:type schema:Organization
285 https://www.grid.ac/institutes/grid.452611.5 schema:alternateName Japan International Research Center for Agricultural Sciences
286 schema:name Biological Resources Division, Japan International Research Center for Agricultural Sciences, Ibaraki 305-8686, Japan
287 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...