Direct reprogramming of human neural stem cells by OCT4 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-10

AUTHORS

Jeong Beom Kim, Boris Greber, Marcos J. Araúzo-Bravo, Johann Meyer, Kook In Park, Holm Zaehres, Hans R. Schöler

ABSTRACT

Induced pluripotent stem (iPS) cells have been generated from mouse and human somatic cells by ectopic expression of four transcription factors (OCT4 (also called POU5F1), SOX2, c-Myc and KLF4). We previously reported that Oct4 alone is sufficient to reprogram directly adult mouse neural stem cells to iPS cells. Here we report the generation of one-factor human iPS cells from human fetal neural stem cells (one-factor (1F) human NiPS cells) by ectopic expression of OCT4 alone. One-factor human NiPS cells resemble human embryonic stem cells in global gene expression profiles, epigenetic status, as well as pluripotency in vitro and in vivo. These findings demonstrate that the transcription factor OCT4 is sufficient to reprogram human neural stem cells to pluripotency. One-factor iPS cell generation will advance the field further towards understanding reprogramming and generating patient-specific pluripotent stem cells. More... »

PAGES

649

References to SciGraph publications

Journal

TITLE

Nature

ISSUE

7264

VOLUME

461

Related Patents

  • Generating Induced Pluripotent Stem Cells And Progenitor Cells From Fibroblasts
  • Methods And Products For Transfection
  • Methods And Products For Transfecting Cells
  • Protein-Induced Pluripotent Cell Technology And Uses Thereof
  • Methods And Products For Expressing Proteins In Cells
  • Generation Of Lineage-Restricted Progenitor Cells From Differentiated Cells
  • Methods And Products For Expressing Proteins In Cells
  • Methods And Products For Expressing Proteins In Cells
  • A Protein-Induced Pluripotent Cell Technology Uses Thereof
  • Methods And Products For Transfecting Cells
  • Methods And Products For Nucleic Acid Production And Delivery
  • Methods And Products For Expressing Proteins In Cells
  • Human Blood-Brain Endothelial Cells Derived From Pluripotent Stem Cells And Blood-Brain Barrier Model Thereof
  • Bacterial Mediated Delivery Of Nuclear Protein Into Pluripotent And Differentiated Cells
  • Methods And Products For Transfection
  • Methods And Products For Transfection
  • Methods, Systems And Compositions Relating To Cell Conversion Via Protein-Induced In-Vivo Cell Reprogramming
  • Nuclear Reprogrammed Cells Generated By Introduction Of A Histone H2aa Or Th2a Gene, A Histone H2ba Or Th2b Gene, Or A Phosphorylation-Mimic Of Histone Chaperon Npm2 Gene, An Oct Family Gene And A Klf Family Gene Into A Mammalian Somatic Cell
  • Methods And Products For Transfecting Cells
  • Method For Generating Pancreatic Hormone-Producing Cells
  • Methods And Products For Expressing Proteins In Cells
  • Cell Culture Platform For Single Cell Sorting And Enhanced Reprogramming Of Ipscs
  • Epigenomic Induced Pluripotent Stem Cell Signatures
  • Reprogramming Cells To A Less Differentiated State
  • Methods Of Reprogramming Cells To A Less Differentiated State
  • Nucleic Acid Expression Construct And Its Use As A Cell Proliferation Marker
  • Methods And Products For Reprogramming Cells To A Less Differentiated State
  • Products For Transfection And Reprogramming
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nature08436

    DOI

    http://dx.doi.org/10.1038/nature08436

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1002503252

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/19718018


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Biomarkers", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Dedifferentiation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Differentiation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Line", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cellular Reprogramming", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA Methylation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Embryonic Stem Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Epigenesis, Genetic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Fetus", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression Profiling", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Germ Layers", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Neurons", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Octamer Transcription Factor-3", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Pluripotent Stem Cells", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Max Planck Institute for Molecular Biomedicine", 
              "id": "https://www.grid.ac/institutes/grid.461801.a", 
              "name": [
                "Max Planck Institute for Molecular Biomedicine, Department of Cell and Developmental Biology, R\u00f6ntgenstrasse 20, 48149 M\u00fcnster, NRW, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kim", 
            "givenName": "Jeong Beom", 
            "id": "sg:person.01010735310.80", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01010735310.80"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Max Planck Institute for Molecular Biomedicine", 
              "id": "https://www.grid.ac/institutes/grid.461801.a", 
              "name": [
                "Max Planck Institute for Molecular Biomedicine, Department of Cell and Developmental Biology, R\u00f6ntgenstrasse 20, 48149 M\u00fcnster, NRW, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Greber", 
            "givenName": "Boris", 
            "id": "sg:person.01266631275.60", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01266631275.60"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Max Planck Institute for Molecular Biomedicine", 
              "id": "https://www.grid.ac/institutes/grid.461801.a", 
              "name": [
                "Max Planck Institute for Molecular Biomedicine, Department of Cell and Developmental Biology, R\u00f6ntgenstrasse 20, 48149 M\u00fcnster, NRW, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ara\u00fazo-Bravo", 
            "givenName": "Marcos J.", 
            "id": "sg:person.0665400112.20", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0665400112.20"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Hannover Medical School", 
              "id": "https://www.grid.ac/institutes/grid.10423.34", 
              "name": [
                "Hannover Medical School, Department of Experimental Hematology, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Meyer", 
            "givenName": "Johann", 
            "id": "sg:person.01211135755.62", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01211135755.62"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Yonsei University", 
              "id": "https://www.grid.ac/institutes/grid.15444.30", 
              "name": [
                "Department of Pediatrics, BK21 Project for Medical Sciences, Yonsei University College of Medicine, 250 Seongsanno, Seodaemoon-gu, Seoul 120-752, Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Park", 
            "givenName": "Kook In", 
            "id": "sg:person.011645647334.84", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011645647334.84"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Max Planck Institute for Molecular Biomedicine", 
              "id": "https://www.grid.ac/institutes/grid.461801.a", 
              "name": [
                "Max Planck Institute for Molecular Biomedicine, Department of Cell and Developmental Biology, R\u00f6ntgenstrasse 20, 48149 M\u00fcnster, NRW, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zaehres", 
            "givenName": "Holm", 
            "id": "sg:person.0644100423.05", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0644100423.05"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Max Planck Institute for Molecular Biomedicine", 
              "id": "https://www.grid.ac/institutes/grid.461801.a", 
              "name": [
                "Max Planck Institute for Molecular Biomedicine, Department of Cell and Developmental Biology, R\u00f6ntgenstrasse 20, 48149 M\u00fcnster, NRW, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sch\u00f6ler", 
            "givenName": "Hans R.", 
            "id": "sg:person.01172213120.10", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01172213120.10"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/j.stem.2009.05.005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000666854"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0076-6879(06)20004-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002232341"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature05934", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002347899", 
              "https://doi.org/10.1038/nature05934"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1374", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002744128", 
              "https://doi.org/10.1038/nbt1374"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0140-6736(04)16627-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005522228"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2008.05.011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006466689"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/stem.104", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009216037"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/stem.104", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009216037"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2007.11.019", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010904856"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature06534", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011992920", 
              "https://doi.org/10.1038/nature06534"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2007.12.001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012875023"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2006.07.024", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014573758"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature07061", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017686107", 
              "https://doi.org/10.1038/nature07061"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature05944", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019109862", 
              "https://doi.org/10.1038/nature05944"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2009.01.023", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022080492"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt751", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022131564", 
              "https://doi.org/10.1038/nbt751"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt751", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022131564", 
              "https://doi.org/10.1038/nbt751"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0711983105", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024490582"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.1503", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026252022", 
              "https://doi.org/10.1038/nbt.1503"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.282.5391.1145", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028898955"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2007.05.014", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029649252"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1072530", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037567645"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1158799", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039463429"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1154884", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041856503"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1151526", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048905674"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.expneurol.2006.03.015", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049540429"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.1502", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051466228", 
              "https://doi.org/10.1038/nbt.1502"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2009-10", 
        "datePublishedReg": "2009-10-01", 
        "description": "Induced pluripotent stem (iPS) cells have been generated from mouse and human somatic cells by ectopic expression of four transcription factors (OCT4 (also called POU5F1), SOX2, c-Myc and KLF4). We previously reported that Oct4 alone is sufficient to reprogram directly adult mouse neural stem cells to iPS cells. Here we report the generation of one-factor human iPS cells from human fetal neural stem cells (one-factor (1F) human NiPS cells) by ectopic expression of OCT4 alone. One-factor human NiPS cells resemble human embryonic stem cells in global gene expression profiles, epigenetic status, as well as pluripotency in vitro and in vivo. These findings demonstrate that the transcription factor OCT4 is sufficient to reprogram human neural stem cells to pluripotency. One-factor iPS cell generation will advance the field further towards understanding reprogramming and generating patient-specific pluripotent stem cells.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nature08436", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1018957", 
            "issn": [
              "0090-0028", 
              "1476-4687"
            ], 
            "name": "Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "7264", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "461"
          }
        ], 
        "name": "Direct reprogramming of human neural stem cells by OCT4", 
        "pagination": "649", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "0beaf0610b1251ee14bd4e9cf240422751d45440749551b0be146b15f69c239e"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "19718018"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "0410462"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nature08436"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1002503252"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nature08436", 
          "https://app.dimensions.ai/details/publication/pub.1002503252"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T15:38", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8664_00000421.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://www.nature.com/articles/nature08436"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature08436'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature08436'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature08436'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature08436'


     

    This table displays all metadata directly associated to this object as RDF triples.

    268 TRIPLES      21 PREDICATES      71 URIs      38 LITERALS      26 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nature08436 schema:about N2c6cc9706d4a44d7bd3b5859eb3c791b
    2 N2cd7b14f403e429a850afc67d8369861
    3 N41869000eebb4ee38e1a3d6270484b19
    4 N48b683373bb041fcb0d93c591b6e7d7c
    5 N5851453a8fb24498bc36d3f57632e79a
    6 N62f7ef283c4f40a998c1c07432e02e1a
    7 N777c4e293aa24adf8874aeaba7889e63
    8 N89b2e57d447d4390bbd71350412f11c8
    9 Na38772886d0a478bb5f1dfddbe86b2a9
    10 Nc2a2983862f040a6a268c6a49dd1ac9b
    11 Nc76653bedcf44e5c950e1b33fb9def62
    12 Ncde9e9bbe0c6471198cf30e264060b95
    13 Nceede71ef55a4a2999579cd8e6e4cacc
    14 Nd5f101ee2c67439f8c9b431728ce1d27
    15 Ne62240f0409a4c0abd369364799f2af4
    16 Ne984a9b41e114c8c93ffc592d711e1d2
    17 Nfa193ce01a6843689092f1a3111ca934
    18 anzsrc-for:06
    19 anzsrc-for:0604
    20 schema:author N1d5adf38ead04dcba0e639b418ecb7cb
    21 schema:citation sg:pub.10.1038/nature05934
    22 sg:pub.10.1038/nature05944
    23 sg:pub.10.1038/nature06534
    24 sg:pub.10.1038/nature07061
    25 sg:pub.10.1038/nbt.1502
    26 sg:pub.10.1038/nbt.1503
    27 sg:pub.10.1038/nbt1374
    28 sg:pub.10.1038/nbt751
    29 https://doi.org/10.1002/stem.104
    30 https://doi.org/10.1016/j.cell.2006.07.024
    31 https://doi.org/10.1016/j.cell.2007.11.019
    32 https://doi.org/10.1016/j.cell.2009.01.023
    33 https://doi.org/10.1016/j.expneurol.2006.03.015
    34 https://doi.org/10.1016/j.stem.2007.05.014
    35 https://doi.org/10.1016/j.stem.2007.12.001
    36 https://doi.org/10.1016/j.stem.2008.05.011
    37 https://doi.org/10.1016/j.stem.2009.05.005
    38 https://doi.org/10.1016/s0076-6879(06)20004-1
    39 https://doi.org/10.1016/s0140-6736(04)16627-0
    40 https://doi.org/10.1073/pnas.0711983105
    41 https://doi.org/10.1126/science.1072530
    42 https://doi.org/10.1126/science.1151526
    43 https://doi.org/10.1126/science.1154884
    44 https://doi.org/10.1126/science.1158799
    45 https://doi.org/10.1126/science.282.5391.1145
    46 schema:datePublished 2009-10
    47 schema:datePublishedReg 2009-10-01
    48 schema:description Induced pluripotent stem (iPS) cells have been generated from mouse and human somatic cells by ectopic expression of four transcription factors (OCT4 (also called POU5F1), SOX2, c-Myc and KLF4). We previously reported that Oct4 alone is sufficient to reprogram directly adult mouse neural stem cells to iPS cells. Here we report the generation of one-factor human iPS cells from human fetal neural stem cells (one-factor (1F) human NiPS cells) by ectopic expression of OCT4 alone. One-factor human NiPS cells resemble human embryonic stem cells in global gene expression profiles, epigenetic status, as well as pluripotency in vitro and in vivo. These findings demonstrate that the transcription factor OCT4 is sufficient to reprogram human neural stem cells to pluripotency. One-factor iPS cell generation will advance the field further towards understanding reprogramming and generating patient-specific pluripotent stem cells.
    49 schema:genre research_article
    50 schema:inLanguage en
    51 schema:isAccessibleForFree false
    52 schema:isPartOf N3b78430f31de49b9a1a6249176b93ed5
    53 N3f04d728305a400698c230f050f1b8d7
    54 sg:journal.1018957
    55 schema:name Direct reprogramming of human neural stem cells by OCT4
    56 schema:pagination 649
    57 schema:productId N00fda2937adf4ffd8431d15835782603
    58 N0d899d4c22664bc697ad9b1adce9ae3d
    59 N4766b2517e294665a723719434976174
    60 N857c264209714220bac8104834d81eae
    61 N9112f4b5aeb94b2b866074f983563c60
    62 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002503252
    63 https://doi.org/10.1038/nature08436
    64 schema:sdDatePublished 2019-04-10T15:38
    65 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    66 schema:sdPublisher N4a19d8ddbd504d29a7322b29a6b49064
    67 schema:url https://www.nature.com/articles/nature08436
    68 sgo:license sg:explorer/license/
    69 sgo:sdDataset articles
    70 rdf:type schema:ScholarlyArticle
    71 N00fda2937adf4ffd8431d15835782603 schema:name doi
    72 schema:value 10.1038/nature08436
    73 rdf:type schema:PropertyValue
    74 N0d899d4c22664bc697ad9b1adce9ae3d schema:name readcube_id
    75 schema:value 0beaf0610b1251ee14bd4e9cf240422751d45440749551b0be146b15f69c239e
    76 rdf:type schema:PropertyValue
    77 N1d5adf38ead04dcba0e639b418ecb7cb rdf:first sg:person.01010735310.80
    78 rdf:rest N53ba43eaf0cf461f8bfe0ad1af4f06ed
    79 N244afb999c0742398a41970501d2db25 rdf:first sg:person.01211135755.62
    80 rdf:rest N34d0914065de4be9934464a9c6b6a9ab
    81 N2c6cc9706d4a44d7bd3b5859eb3c791b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    82 schema:name Gene Expression Profiling
    83 rdf:type schema:DefinedTerm
    84 N2cd7b14f403e429a850afc67d8369861 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    85 schema:name Neurons
    86 rdf:type schema:DefinedTerm
    87 N34d0914065de4be9934464a9c6b6a9ab rdf:first sg:person.011645647334.84
    88 rdf:rest N9d11aed2c4c543afa335651298e1f41d
    89 N3b78430f31de49b9a1a6249176b93ed5 schema:volumeNumber 461
    90 rdf:type schema:PublicationVolume
    91 N3f04d728305a400698c230f050f1b8d7 schema:issueNumber 7264
    92 rdf:type schema:PublicationIssue
    93 N41869000eebb4ee38e1a3d6270484b19 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    94 schema:name Cellular Reprogramming
    95 rdf:type schema:DefinedTerm
    96 N4766b2517e294665a723719434976174 schema:name nlm_unique_id
    97 schema:value 0410462
    98 rdf:type schema:PropertyValue
    99 N48b683373bb041fcb0d93c591b6e7d7c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    100 schema:name Mice
    101 rdf:type schema:DefinedTerm
    102 N4a19d8ddbd504d29a7322b29a6b49064 schema:name Springer Nature - SN SciGraph project
    103 rdf:type schema:Organization
    104 N53ba43eaf0cf461f8bfe0ad1af4f06ed rdf:first sg:person.01266631275.60
    105 rdf:rest Ne91e38f3cf4845a8a1a071ec597a8fe0
    106 N5851453a8fb24498bc36d3f57632e79a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    107 schema:name Biomarkers
    108 rdf:type schema:DefinedTerm
    109 N62f7ef283c4f40a998c1c07432e02e1a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name Pluripotent Stem Cells
    111 rdf:type schema:DefinedTerm
    112 N777c4e293aa24adf8874aeaba7889e63 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    113 schema:name Fetus
    114 rdf:type schema:DefinedTerm
    115 N857c264209714220bac8104834d81eae schema:name dimensions_id
    116 schema:value pub.1002503252
    117 rdf:type schema:PropertyValue
    118 N89b2e57d447d4390bbd71350412f11c8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    119 schema:name Epigenesis, Genetic
    120 rdf:type schema:DefinedTerm
    121 N9112f4b5aeb94b2b866074f983563c60 schema:name pubmed_id
    122 schema:value 19718018
    123 rdf:type schema:PropertyValue
    124 N9d11aed2c4c543afa335651298e1f41d rdf:first sg:person.0644100423.05
    125 rdf:rest Nbff910cbbbd74f8cb74fac29126a2324
    126 Na38772886d0a478bb5f1dfddbe86b2a9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name Animals
    128 rdf:type schema:DefinedTerm
    129 Nbff910cbbbd74f8cb74fac29126a2324 rdf:first sg:person.01172213120.10
    130 rdf:rest rdf:nil
    131 Nc2a2983862f040a6a268c6a49dd1ac9b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    132 schema:name Cell Line
    133 rdf:type schema:DefinedTerm
    134 Nc76653bedcf44e5c950e1b33fb9def62 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    135 schema:name Germ Layers
    136 rdf:type schema:DefinedTerm
    137 Ncde9e9bbe0c6471198cf30e264060b95 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    138 schema:name Octamer Transcription Factor-3
    139 rdf:type schema:DefinedTerm
    140 Nceede71ef55a4a2999579cd8e6e4cacc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    141 schema:name Humans
    142 rdf:type schema:DefinedTerm
    143 Nd5f101ee2c67439f8c9b431728ce1d27 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    144 schema:name Embryonic Stem Cells
    145 rdf:type schema:DefinedTerm
    146 Ne62240f0409a4c0abd369364799f2af4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    147 schema:name DNA Methylation
    148 rdf:type schema:DefinedTerm
    149 Ne91e38f3cf4845a8a1a071ec597a8fe0 rdf:first sg:person.0665400112.20
    150 rdf:rest N244afb999c0742398a41970501d2db25
    151 Ne984a9b41e114c8c93ffc592d711e1d2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    152 schema:name Cell Dedifferentiation
    153 rdf:type schema:DefinedTerm
    154 Nfa193ce01a6843689092f1a3111ca934 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    155 schema:name Cell Differentiation
    156 rdf:type schema:DefinedTerm
    157 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    158 schema:name Biological Sciences
    159 rdf:type schema:DefinedTerm
    160 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    161 schema:name Genetics
    162 rdf:type schema:DefinedTerm
    163 sg:journal.1018957 schema:issn 0090-0028
    164 1476-4687
    165 schema:name Nature
    166 rdf:type schema:Periodical
    167 sg:person.01010735310.80 schema:affiliation https://www.grid.ac/institutes/grid.461801.a
    168 schema:familyName Kim
    169 schema:givenName Jeong Beom
    170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01010735310.80
    171 rdf:type schema:Person
    172 sg:person.011645647334.84 schema:affiliation https://www.grid.ac/institutes/grid.15444.30
    173 schema:familyName Park
    174 schema:givenName Kook In
    175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011645647334.84
    176 rdf:type schema:Person
    177 sg:person.01172213120.10 schema:affiliation https://www.grid.ac/institutes/grid.461801.a
    178 schema:familyName Schöler
    179 schema:givenName Hans R.
    180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01172213120.10
    181 rdf:type schema:Person
    182 sg:person.01211135755.62 schema:affiliation https://www.grid.ac/institutes/grid.10423.34
    183 schema:familyName Meyer
    184 schema:givenName Johann
    185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01211135755.62
    186 rdf:type schema:Person
    187 sg:person.01266631275.60 schema:affiliation https://www.grid.ac/institutes/grid.461801.a
    188 schema:familyName Greber
    189 schema:givenName Boris
    190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01266631275.60
    191 rdf:type schema:Person
    192 sg:person.0644100423.05 schema:affiliation https://www.grid.ac/institutes/grid.461801.a
    193 schema:familyName Zaehres
    194 schema:givenName Holm
    195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0644100423.05
    196 rdf:type schema:Person
    197 sg:person.0665400112.20 schema:affiliation https://www.grid.ac/institutes/grid.461801.a
    198 schema:familyName Araúzo-Bravo
    199 schema:givenName Marcos J.
    200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0665400112.20
    201 rdf:type schema:Person
    202 sg:pub.10.1038/nature05934 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002347899
    203 https://doi.org/10.1038/nature05934
    204 rdf:type schema:CreativeWork
    205 sg:pub.10.1038/nature05944 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019109862
    206 https://doi.org/10.1038/nature05944
    207 rdf:type schema:CreativeWork
    208 sg:pub.10.1038/nature06534 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011992920
    209 https://doi.org/10.1038/nature06534
    210 rdf:type schema:CreativeWork
    211 sg:pub.10.1038/nature07061 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017686107
    212 https://doi.org/10.1038/nature07061
    213 rdf:type schema:CreativeWork
    214 sg:pub.10.1038/nbt.1502 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051466228
    215 https://doi.org/10.1038/nbt.1502
    216 rdf:type schema:CreativeWork
    217 sg:pub.10.1038/nbt.1503 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026252022
    218 https://doi.org/10.1038/nbt.1503
    219 rdf:type schema:CreativeWork
    220 sg:pub.10.1038/nbt1374 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002744128
    221 https://doi.org/10.1038/nbt1374
    222 rdf:type schema:CreativeWork
    223 sg:pub.10.1038/nbt751 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022131564
    224 https://doi.org/10.1038/nbt751
    225 rdf:type schema:CreativeWork
    226 https://doi.org/10.1002/stem.104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009216037
    227 rdf:type schema:CreativeWork
    228 https://doi.org/10.1016/j.cell.2006.07.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014573758
    229 rdf:type schema:CreativeWork
    230 https://doi.org/10.1016/j.cell.2007.11.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010904856
    231 rdf:type schema:CreativeWork
    232 https://doi.org/10.1016/j.cell.2009.01.023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022080492
    233 rdf:type schema:CreativeWork
    234 https://doi.org/10.1016/j.expneurol.2006.03.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049540429
    235 rdf:type schema:CreativeWork
    236 https://doi.org/10.1016/j.stem.2007.05.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029649252
    237 rdf:type schema:CreativeWork
    238 https://doi.org/10.1016/j.stem.2007.12.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012875023
    239 rdf:type schema:CreativeWork
    240 https://doi.org/10.1016/j.stem.2008.05.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006466689
    241 rdf:type schema:CreativeWork
    242 https://doi.org/10.1016/j.stem.2009.05.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000666854
    243 rdf:type schema:CreativeWork
    244 https://doi.org/10.1016/s0076-6879(06)20004-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002232341
    245 rdf:type schema:CreativeWork
    246 https://doi.org/10.1016/s0140-6736(04)16627-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005522228
    247 rdf:type schema:CreativeWork
    248 https://doi.org/10.1073/pnas.0711983105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024490582
    249 rdf:type schema:CreativeWork
    250 https://doi.org/10.1126/science.1072530 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037567645
    251 rdf:type schema:CreativeWork
    252 https://doi.org/10.1126/science.1151526 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048905674
    253 rdf:type schema:CreativeWork
    254 https://doi.org/10.1126/science.1154884 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041856503
    255 rdf:type schema:CreativeWork
    256 https://doi.org/10.1126/science.1158799 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039463429
    257 rdf:type schema:CreativeWork
    258 https://doi.org/10.1126/science.282.5391.1145 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028898955
    259 rdf:type schema:CreativeWork
    260 https://www.grid.ac/institutes/grid.10423.34 schema:alternateName Hannover Medical School
    261 schema:name Hannover Medical School, Department of Experimental Hematology, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany
    262 rdf:type schema:Organization
    263 https://www.grid.ac/institutes/grid.15444.30 schema:alternateName Yonsei University
    264 schema:name Department of Pediatrics, BK21 Project for Medical Sciences, Yonsei University College of Medicine, 250 Seongsanno, Seodaemoon-gu, Seoul 120-752, Korea
    265 rdf:type schema:Organization
    266 https://www.grid.ac/institutes/grid.461801.a schema:alternateName Max Planck Institute for Molecular Biomedicine
    267 schema:name Max Planck Institute for Molecular Biomedicine, Department of Cell and Developmental Biology, Röntgenstrasse 20, 48149 Münster, NRW, Germany
    268 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...