Precise genome modification in the crop species Zea mays using zinc-finger nucleases View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-04-29

AUTHORS

Vipula K. Shukla, Yannick Doyon, Jeffrey C. Miller, Russell C. DeKelver, Erica A. Moehle, Sarah E. Worden, Jon C. Mitchell, Nicole L. Arnold, Sunita Gopalan, Xiangdong Meng, Vivian M. Choi, Jeremy M. Rock, Ying-Ying Wu, George E. Katibah, Gao Zhifang, David McCaskill, Matthew A. Simpson, Beth Blakeslee, Scott A. Greenwalt, Holly J. Butler, Sarah J. Hinkley, Lei Zhang, Edward J. Rebar, Philip D. Gregory, Fyodor D. Urnov

ABSTRACT

On target for new plant genesThe scope for improvement of yield and disease resistance of crop plants by genetic engineering has been limited by the lack of an efficient method for targeted gene modification. Zinc-finger protein technology looks set to fill the gap. This relies on the use of designed zinc-finger nucleases, artificial chimaeric proteins that exploit the natural recognition mechanism of cellular DNA repair machinery, to make sequence-specific double-stranded DNA breaks at a target locus. In this issue two groups report the successful application of this emerging technique. Shukla et al. modify the maize gene IPK1, thereby introducing both herbicide tolerance and modified phytate metabolism into this important crop plant. Townsend et al. target the SuR loci in tobacco plants, conferring resistance to imidazolinone and sulphonylurea herbicides. The method achieves a high frequency of gene targeting and should be suitable for the routine modification of endogenous plant genes. More... »

PAGES

437-441

References to SciGraph publications

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  • Journal

    TITLE

    Nature

    ISSUE

    7245

    VOLUME

    459

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nature07992

    DOI

    http://dx.doi.org/10.1038/nature07992

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1028276115

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/19404259


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