Single Lgr5 stem cells build crypt–villus structures in vitro without a mesenchymal niche View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-05

AUTHORS

Toshiro Sato, Robert G. Vries, Hugo J. Snippert, Marc van de Wetering, Nick Barker, Daniel E. Stange, Johan H. van Es, Arie Abo, Pekka Kujala, Peter J. Peters, Hans Clevers

ABSTRACT

The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently demonstrated the presence of about six cycling Lgr5(+) stem cells at the bottoms of small-intestinal crypts. Here we describe the establishment of long-term culture conditions under which single crypts undergo multiple crypt fission events, while simultanously generating villus-like epithelial domains in which all differentiated cell types are present. Single sorted Lgr5(+) stem cells can also initiate these cryptvillus organoids. Tracing experiments indicate that the Lgr5(+) stem-cell hierarchy is maintained in organoids. We conclude that intestinal cryptvillus units are self-organizing structures, which can be built from a single stem cell in the absence of a non-epithelial cellular niche. More... »

PAGES

262

Journal

TITLE

Nature

ISSUE

7244

VOLUME

459

Related Patents

  • Methods And Systems For Converting Precursor Cells Into Intestinal Tissues Through Directed Differentiation
  • A Nod2-Dependent Pathway Of Cytoprotection Of Stem Cells
  • Methods Of Analysing Homeostatic State In Cell Systems
  • Liquid Culturing Of Epithelial Stem Cells
  • Compositions And Methods Relating To Induction Of Intestinal Stem Cell Homeogenesis And/Or Regeneration
  • Culture Medium For Epithelial Stem Cells And Organoids Comprising Said Stem Cells.
  • A Rapid Quantitative Assay To Measure Cftr Function In A Primary Intestinal Culture Model
  • Technique For Isolating/Culturing Colorectal Epithelial Stem Cell, And Technique For Transplanting Colorectal Epithelium Employing Said Technique
  • Compounds
  • Liver Organoid, Uses Thereof And Culture Method For Obtaining Them
  • Organoid Arrays
  • Organoid Tissue Engineering
  • A Nod2-Dependant Pathway Of Cytoprotection Of Stem Cells
  • Compounds And Pharmaceutical Compositions Thereof For Use In The Treatment Of Cystic Fibrosis
  • Methods And Compositions Of P27kip1 Transcriptional Modulators
  • Culture Medium For Epithelial Stem Cells And Organoids Comprising The Stem Cells
  • Cancer Stem Cell Isolation
  • Cancer Stem Cell-Specific Molecule
  • A Rapid Quantitative Assay To Measure Cftr Function In A Primary Intestinal Culture Model
  • Organoid Tissue Engineering
  • Organoid Arrays
  • Fluidic Device And Perfusion System For In Vitro Complex Living Tissue Reconstruction
  • Rapid Quantitative Assay To Measure Cftr Function In A Primary Intestinal Culture Model
  • Production Of Differentiated Enteroendocrine Cells And Insulin Producing Cells
  • Methods For Establishing And Improving The Survival Of A Population Of Pancreatic Progenitor Or Stem Cells
  • Culture Medium For Epithelial Stem Cells And Organoids Comprising Said Stem Cells
  • Culture Media For Stem Cells
  • Method For Culture Of Human And Mouse Prostate Organoids And Uses Thereof
  • Improved Culture Method For Organoids
  • Three Dimensional Hydrogels For Culturing Organoids
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nature07935

    DOI

    http://dx.doi.org/10.1038/nature07935

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1024082069

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/19329995


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Culture Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Lineage", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Separation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression Regulation, Developmental", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Intestinal Mucosa", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Intestines", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mesoderm", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Multipotent Stem Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Organoids", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Paneth Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Receptors, G-Protein-Coupled", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Receptors, Notch", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Regeneration", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Stem Cell Niche", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Stem Cells", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Hubrecht Institute", 
              "id": "https://www.grid.ac/institutes/grid.419927.0", 
              "name": [
                "Hubrecht Institute and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sato", 
            "givenName": "Toshiro", 
            "id": "sg:person.0720623733.65", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0720623733.65"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Hubrecht Institute", 
              "id": "https://www.grid.ac/institutes/grid.419927.0", 
              "name": [
                "Hubrecht Institute and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Vries", 
            "givenName": "Robert G.", 
            "id": "sg:person.01123453474.42", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123453474.42"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Hubrecht Institute", 
              "id": "https://www.grid.ac/institutes/grid.419927.0", 
              "name": [
                "Hubrecht Institute and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Snippert", 
            "givenName": "Hugo J.", 
            "id": "sg:person.0623545141.20", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0623545141.20"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Hubrecht Institute", 
              "id": "https://www.grid.ac/institutes/grid.419927.0", 
              "name": [
                "Hubrecht Institute and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "van de Wetering", 
            "givenName": "Marc", 
            "id": "sg:person.0750240753.37", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0750240753.37"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Hubrecht Institute", 
              "id": "https://www.grid.ac/institutes/grid.419927.0", 
              "name": [
                "Hubrecht Institute and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Barker", 
            "givenName": "Nick", 
            "id": "sg:person.01307053755.26", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307053755.26"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Hubrecht Institute", 
              "id": "https://www.grid.ac/institutes/grid.419927.0", 
              "name": [
                "Hubrecht Institute and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Stange", 
            "givenName": "Daniel E.", 
            "id": "sg:person.0643614125.13", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0643614125.13"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Hubrecht Institute", 
              "id": "https://www.grid.ac/institutes/grid.419927.0", 
              "name": [
                "Hubrecht Institute and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "van Es", 
            "givenName": "Johan H.", 
            "id": "sg:person.01064467353.67", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01064467353.67"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Nuvelo, Inc., 201 Industrial Road, Suite 310, San Carlos, California 94070-6211, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Abo", 
            "givenName": "Arie", 
            "id": "sg:person.01216542135.65", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01216542135.65"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Antoni van Leeuwenhoek Hospital", 
              "id": "https://www.grid.ac/institutes/grid.430814.a", 
              "name": [
                "The Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kujala", 
            "givenName": "Pekka", 
            "id": "sg:person.0616060543.03", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0616060543.03"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Antoni van Leeuwenhoek Hospital", 
              "id": "https://www.grid.ac/institutes/grid.430814.a", 
              "name": [
                "The Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Peters", 
            "givenName": "Peter J.", 
            "id": "sg:person.01132006272.03", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132006272.03"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Hubrecht Institute", 
              "id": "https://www.grid.ac/institutes/grid.419927.0", 
              "name": [
                "Hubrecht Institute and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Clevers", 
            "givenName": "Hans", 
            "id": "sg:person.0662243670.45", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0662243670.45"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1006/excr.1996.0145", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001447364"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/1270", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002695839", 
              "https://doi.org/10.1038/1270"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/1270", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002695839", 
              "https://doi.org/10.1038/1270"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/00042737-200107000-00002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003155995"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/00042737-200107000-00002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003155995"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1074-7613(00)80457-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003664648"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12015-006-0048-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006358707", 
              "https://doi.org/10.1007/s12015-006-0048-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s12015-006-0048-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006358707", 
              "https://doi.org/10.1007/s12015-006-0048-1"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1053/j.gastro.2006.11.017", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006367821"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1749-6632.1998.tb11107.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008007276"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1749-6632.1998.tb11107.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008007276"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gad.267103", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011480346"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gad.267103", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011480346"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gad.1674008", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011867533"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1023/a:1010615124301", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013545902", 
              "https://doi.org/10.1023/a:1010615124301"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1093587", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014414968"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0076-6879(06)19014-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016186813"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0092-8674(02)01015-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018479938"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-2184.1985.tb00655.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021506214"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/35102160", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021584545", 
              "https://doi.org/10.1038/35102160"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/35102160", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021584545", 
              "https://doi.org/10.1038/35102160"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1310", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024578139", 
              "https://doi.org/10.1038/nbt1310"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/5007", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030839891", 
              "https://doi.org/10.1038/5007"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/5007", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030839891", 
              "https://doi.org/10.1038/5007"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature04496", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040185730", 
              "https://doi.org/10.1038/nature04496"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature04496", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040185730", 
              "https://doi.org/10.1038/nature04496"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature04496", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040185730", 
              "https://doi.org/10.1038/nature04496"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-213x-1-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042905963", 
              "https://doi.org/10.1186/1471-213x-1-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0016-5085(99)70344-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044163755"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0016-5085(99)70344-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044163755"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/aja.1001410403", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045687593"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature03659", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046707660", 
              "https://doi.org/10.1038/nature03659"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature03659", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046707660", 
              "https://doi.org/10.1038/nature03659"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.cellbio.21.012704.131525", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047691540"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.cellbio.21.012704.131525", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047691540"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature06196", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050225537", 
              "https://doi.org/10.1038/nature06196"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.2536800100", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050279034"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.2536800100", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050279034"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/excr.2002.5499", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050399692"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1112521", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050697196"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1152/ajpcell.1999.277.2.c183", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1074506095"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1075305875", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1077003237", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2009-05", 
        "datePublishedReg": "2009-05-01", 
        "description": "The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently demonstrated the presence of about six cycling Lgr5(+) stem cells at the bottoms of small-intestinal crypts. Here we describe the establishment of long-term culture conditions under which single crypts undergo multiple crypt fission events, while simultanously generating villus-like epithelial domains in which all differentiated cell types are present. Single sorted Lgr5(+) stem cells can also initiate these cryptvillus organoids. Tracing experiments indicate that the Lgr5(+) stem-cell hierarchy is maintained in organoids. We conclude that intestinal cryptvillus units are self-organizing structures, which can be built from a single stem cell in the absence of a non-epithelial cellular niche.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nature07935", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1018957", 
            "issn": [
              "0090-0028", 
              "1476-4687"
            ], 
            "name": "Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "7244", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "459"
          }
        ], 
        "name": "Single Lgr5 stem cells build crypt\u2013villus structures in vitro without a mesenchymal niche", 
        "pagination": "262", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "72bba065a24b94be0b8fa347b26c26dd6e820e88f6621845f37e3cb020dbe2ab"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "19329995"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "0410462"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nature07935"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1024082069"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nature07935", 
          "https://app.dimensions.ai/details/publication/pub.1024082069"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T09:10", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000338_0000000338/records_47968_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://www.nature.com/articles/nature07935"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature07935'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature07935'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature07935'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature07935'


     

    This table displays all metadata directly associated to this object as RDF triples.

    310 TRIPLES      21 PREDICATES      76 URIs      38 LITERALS      26 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nature07935 schema:about N0576f4953cb64dce8d4d588e1361b251
    2 N353bb26e56ec44c08bb97632743ec979
    3 N35506e5539614c8c88de7f92bd5eed58
    4 N59cb5aeece50439db418f97ba648f449
    5 N5c1834fff66c4211ac2a609a18b47e7a
    6 N612bf93c349f4ce68ae1b9edd453d865
    7 N62ce6047378e46419d8b5f98858ae6be
    8 N711639dd926e464e9a2578f9f9745908
    9 N7e5e6eea2a554ea7876fcc030b8c4490
    10 N8226bcb2e6cf4173bcc5bd79d0188591
    11 N834ab3e01c23478fb62f246bb2dd4459
    12 N91ea6588f4b844c8a3dbcb06176da138
    13 Nbe85fefca89047e382c2e21aaf210597
    14 Ndbfc3518fb34425d93fa090aadca5f8c
    15 Ndcd331c696d1416d8e24413c375d6bad
    16 Nf861f58d72cc46879032e767311f1a43
    17 Nff01e5d2592647628cf200c7c102d90b
    18 anzsrc-for:06
    19 anzsrc-for:0601
    20 schema:author N25797e1d0ad04217b16997b7ba877628
    21 schema:citation sg:pub.10.1007/s12015-006-0048-1
    22 sg:pub.10.1023/a:1010615124301
    23 sg:pub.10.1038/1270
    24 sg:pub.10.1038/35102160
    25 sg:pub.10.1038/5007
    26 sg:pub.10.1038/nature03659
    27 sg:pub.10.1038/nature04496
    28 sg:pub.10.1038/nature06196
    29 sg:pub.10.1038/nbt1310
    30 sg:pub.10.1186/1471-213x-1-4
    31 https://app.dimensions.ai/details/publication/pub.1075305875
    32 https://app.dimensions.ai/details/publication/pub.1077003237
    33 https://doi.org/10.1002/aja.1001410403
    34 https://doi.org/10.1006/excr.1996.0145
    35 https://doi.org/10.1006/excr.2002.5499
    36 https://doi.org/10.1016/s0016-5085(99)70344-6
    37 https://doi.org/10.1016/s0076-6879(06)19014-x
    38 https://doi.org/10.1016/s0092-8674(02)01015-2
    39 https://doi.org/10.1016/s1074-7613(00)80457-4
    40 https://doi.org/10.1053/j.gastro.2006.11.017
    41 https://doi.org/10.1073/pnas.2536800100
    42 https://doi.org/10.1097/00042737-200107000-00002
    43 https://doi.org/10.1101/gad.1674008
    44 https://doi.org/10.1101/gad.267103
    45 https://doi.org/10.1111/j.1365-2184.1985.tb00655.x
    46 https://doi.org/10.1111/j.1749-6632.1998.tb11107.x
    47 https://doi.org/10.1126/science.1093587
    48 https://doi.org/10.1126/science.1112521
    49 https://doi.org/10.1146/annurev.cellbio.21.012704.131525
    50 https://doi.org/10.1152/ajpcell.1999.277.2.c183
    51 schema:datePublished 2009-05
    52 schema:datePublishedReg 2009-05-01
    53 schema:description The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently demonstrated the presence of about six cycling Lgr5(+) stem cells at the bottoms of small-intestinal crypts. Here we describe the establishment of long-term culture conditions under which single crypts undergo multiple crypt fission events, while simultanously generating villus-like epithelial domains in which all differentiated cell types are present. Single sorted Lgr5(+) stem cells can also initiate these cryptvillus organoids. Tracing experiments indicate that the Lgr5(+) stem-cell hierarchy is maintained in organoids. We conclude that intestinal cryptvillus units are self-organizing structures, which can be built from a single stem cell in the absence of a non-epithelial cellular niche.
    54 schema:genre research_article
    55 schema:inLanguage en
    56 schema:isAccessibleForFree false
    57 schema:isPartOf N7c442d6c635d4411b038fb0b889d0faa
    58 Ne5c0fa5cc2ed480e9ba8dae16318c4f7
    59 sg:journal.1018957
    60 schema:name Single Lgr5 stem cells build crypt–villus structures in vitro without a mesenchymal niche
    61 schema:pagination 262
    62 schema:productId N2d226bdce675497cbbb17c4e43a97bec
    63 N62a4c3ff35f346d3a827a393abdc4006
    64 N6603ad0e11cb4ae2ab3d7e5ed5cff10f
    65 N867f816bd96b4cc2822e50968bbc15a7
    66 Nb61ae69134e64f3dac66d9c69179ad88
    67 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024082069
    68 https://doi.org/10.1038/nature07935
    69 schema:sdDatePublished 2019-04-11T09:10
    70 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    71 schema:sdPublisher Ndfa0b78e510b462cbe3b30f45a49d201
    72 schema:url https://www.nature.com/articles/nature07935
    73 sgo:license sg:explorer/license/
    74 sgo:sdDataset articles
    75 rdf:type schema:ScholarlyArticle
    76 N02bfb53417584c6a9044ae9792851cfe rdf:first sg:person.0643614125.13
    77 rdf:rest Nc272ed69136d4642a55e22847ca4a9e6
    78 N0576f4953cb64dce8d4d588e1361b251 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    79 schema:name Paneth Cells
    80 rdf:type schema:DefinedTerm
    81 N1d54ca26a652425aa31cde416e81ed58 rdf:first sg:person.0750240753.37
    82 rdf:rest N5fc5f65fd1c74447b3dff9d9264671d3
    83 N25797e1d0ad04217b16997b7ba877628 rdf:first sg:person.0720623733.65
    84 rdf:rest Nfc6beaeafefd49dda424e0a7d997df03
    85 N2d226bdce675497cbbb17c4e43a97bec schema:name readcube_id
    86 schema:value 72bba065a24b94be0b8fa347b26c26dd6e820e88f6621845f37e3cb020dbe2ab
    87 rdf:type schema:PropertyValue
    88 N32610ceeaeee400e8cc3bd90f4e32778 schema:name Nuvelo, Inc., 201 Industrial Road, Suite 310, San Carlos, California 94070-6211, USA
    89 rdf:type schema:Organization
    90 N353bb26e56ec44c08bb97632743ec979 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    91 schema:name Animals
    92 rdf:type schema:DefinedTerm
    93 N35506e5539614c8c88de7f92bd5eed58 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    94 schema:name Intestines
    95 rdf:type schema:DefinedTerm
    96 N54ff5483bd384294aaeae711670ca34a rdf:first sg:person.01132006272.03
    97 rdf:rest Nff075a00efe648089aba687cebd7161a
    98 N59cb5aeece50439db418f97ba648f449 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    99 schema:name Cell Lineage
    100 rdf:type schema:DefinedTerm
    101 N5c1834fff66c4211ac2a609a18b47e7a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name Gene Expression Regulation, Developmental
    103 rdf:type schema:DefinedTerm
    104 N5fc5f65fd1c74447b3dff9d9264671d3 rdf:first sg:person.01307053755.26
    105 rdf:rest N02bfb53417584c6a9044ae9792851cfe
    106 N612bf93c349f4ce68ae1b9edd453d865 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    107 schema:name Cell Culture Techniques
    108 rdf:type schema:DefinedTerm
    109 N62a4c3ff35f346d3a827a393abdc4006 schema:name nlm_unique_id
    110 schema:value 0410462
    111 rdf:type schema:PropertyValue
    112 N62ce6047378e46419d8b5f98858ae6be schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    113 schema:name Receptors, Notch
    114 rdf:type schema:DefinedTerm
    115 N6603ad0e11cb4ae2ab3d7e5ed5cff10f schema:name doi
    116 schema:value 10.1038/nature07935
    117 rdf:type schema:PropertyValue
    118 N711639dd926e464e9a2578f9f9745908 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    119 schema:name Multipotent Stem Cells
    120 rdf:type schema:DefinedTerm
    121 N7c442d6c635d4411b038fb0b889d0faa schema:issueNumber 7244
    122 rdf:type schema:PublicationIssue
    123 N7e5e6eea2a554ea7876fcc030b8c4490 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    124 schema:name Intestinal Mucosa
    125 rdf:type schema:DefinedTerm
    126 N8226bcb2e6cf4173bcc5bd79d0188591 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name Mice
    128 rdf:type schema:DefinedTerm
    129 N834ab3e01c23478fb62f246bb2dd4459 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    130 schema:name Stem Cells
    131 rdf:type schema:DefinedTerm
    132 N867f816bd96b4cc2822e50968bbc15a7 schema:name pubmed_id
    133 schema:value 19329995
    134 rdf:type schema:PropertyValue
    135 N91ea6588f4b844c8a3dbcb06176da138 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    136 schema:name Cell Separation
    137 rdf:type schema:DefinedTerm
    138 N96e18064e86043f9bc1941781f32fd52 rdf:first sg:person.0616060543.03
    139 rdf:rest N54ff5483bd384294aaeae711670ca34a
    140 N9e8547ec42b344f59ba7804c9bd2cbb8 rdf:first sg:person.01216542135.65
    141 rdf:rest N96e18064e86043f9bc1941781f32fd52
    142 Nb61ae69134e64f3dac66d9c69179ad88 schema:name dimensions_id
    143 schema:value pub.1024082069
    144 rdf:type schema:PropertyValue
    145 Nbe85fefca89047e382c2e21aaf210597 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    146 schema:name Organoids
    147 rdf:type schema:DefinedTerm
    148 Nc272ed69136d4642a55e22847ca4a9e6 rdf:first sg:person.01064467353.67
    149 rdf:rest N9e8547ec42b344f59ba7804c9bd2cbb8
    150 Nd6f98c861f6c4dc09e4d8c1ca6a74ca7 rdf:first sg:person.0623545141.20
    151 rdf:rest N1d54ca26a652425aa31cde416e81ed58
    152 Ndbfc3518fb34425d93fa090aadca5f8c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    153 schema:name Mesoderm
    154 rdf:type schema:DefinedTerm
    155 Ndcd331c696d1416d8e24413c375d6bad schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    156 schema:name Receptors, G-Protein-Coupled
    157 rdf:type schema:DefinedTerm
    158 Ndfa0b78e510b462cbe3b30f45a49d201 schema:name Springer Nature - SN SciGraph project
    159 rdf:type schema:Organization
    160 Ne5c0fa5cc2ed480e9ba8dae16318c4f7 schema:volumeNumber 459
    161 rdf:type schema:PublicationVolume
    162 Nf861f58d72cc46879032e767311f1a43 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    163 schema:name Stem Cell Niche
    164 rdf:type schema:DefinedTerm
    165 Nfc6beaeafefd49dda424e0a7d997df03 rdf:first sg:person.01123453474.42
    166 rdf:rest Nd6f98c861f6c4dc09e4d8c1ca6a74ca7
    167 Nff01e5d2592647628cf200c7c102d90b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    168 schema:name Regeneration
    169 rdf:type schema:DefinedTerm
    170 Nff075a00efe648089aba687cebd7161a rdf:first sg:person.0662243670.45
    171 rdf:rest rdf:nil
    172 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    173 schema:name Biological Sciences
    174 rdf:type schema:DefinedTerm
    175 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    176 schema:name Biochemistry and Cell Biology
    177 rdf:type schema:DefinedTerm
    178 sg:journal.1018957 schema:issn 0090-0028
    179 1476-4687
    180 schema:name Nature
    181 rdf:type schema:Periodical
    182 sg:person.01064467353.67 schema:affiliation https://www.grid.ac/institutes/grid.419927.0
    183 schema:familyName van Es
    184 schema:givenName Johan H.
    185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01064467353.67
    186 rdf:type schema:Person
    187 sg:person.01123453474.42 schema:affiliation https://www.grid.ac/institutes/grid.419927.0
    188 schema:familyName Vries
    189 schema:givenName Robert G.
    190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123453474.42
    191 rdf:type schema:Person
    192 sg:person.01132006272.03 schema:affiliation https://www.grid.ac/institutes/grid.430814.a
    193 schema:familyName Peters
    194 schema:givenName Peter J.
    195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01132006272.03
    196 rdf:type schema:Person
    197 sg:person.01216542135.65 schema:affiliation N32610ceeaeee400e8cc3bd90f4e32778
    198 schema:familyName Abo
    199 schema:givenName Arie
    200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01216542135.65
    201 rdf:type schema:Person
    202 sg:person.01307053755.26 schema:affiliation https://www.grid.ac/institutes/grid.419927.0
    203 schema:familyName Barker
    204 schema:givenName Nick
    205 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307053755.26
    206 rdf:type schema:Person
    207 sg:person.0616060543.03 schema:affiliation https://www.grid.ac/institutes/grid.430814.a
    208 schema:familyName Kujala
    209 schema:givenName Pekka
    210 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0616060543.03
    211 rdf:type schema:Person
    212 sg:person.0623545141.20 schema:affiliation https://www.grid.ac/institutes/grid.419927.0
    213 schema:familyName Snippert
    214 schema:givenName Hugo J.
    215 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0623545141.20
    216 rdf:type schema:Person
    217 sg:person.0643614125.13 schema:affiliation https://www.grid.ac/institutes/grid.419927.0
    218 schema:familyName Stange
    219 schema:givenName Daniel E.
    220 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0643614125.13
    221 rdf:type schema:Person
    222 sg:person.0662243670.45 schema:affiliation https://www.grid.ac/institutes/grid.419927.0
    223 schema:familyName Clevers
    224 schema:givenName Hans
    225 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0662243670.45
    226 rdf:type schema:Person
    227 sg:person.0720623733.65 schema:affiliation https://www.grid.ac/institutes/grid.419927.0
    228 schema:familyName Sato
    229 schema:givenName Toshiro
    230 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0720623733.65
    231 rdf:type schema:Person
    232 sg:person.0750240753.37 schema:affiliation https://www.grid.ac/institutes/grid.419927.0
    233 schema:familyName van de Wetering
    234 schema:givenName Marc
    235 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0750240753.37
    236 rdf:type schema:Person
    237 sg:pub.10.1007/s12015-006-0048-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006358707
    238 https://doi.org/10.1007/s12015-006-0048-1
    239 rdf:type schema:CreativeWork
    240 sg:pub.10.1023/a:1010615124301 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013545902
    241 https://doi.org/10.1023/a:1010615124301
    242 rdf:type schema:CreativeWork
    243 sg:pub.10.1038/1270 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002695839
    244 https://doi.org/10.1038/1270
    245 rdf:type schema:CreativeWork
    246 sg:pub.10.1038/35102160 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021584545
    247 https://doi.org/10.1038/35102160
    248 rdf:type schema:CreativeWork
    249 sg:pub.10.1038/5007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030839891
    250 https://doi.org/10.1038/5007
    251 rdf:type schema:CreativeWork
    252 sg:pub.10.1038/nature03659 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046707660
    253 https://doi.org/10.1038/nature03659
    254 rdf:type schema:CreativeWork
    255 sg:pub.10.1038/nature04496 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040185730
    256 https://doi.org/10.1038/nature04496
    257 rdf:type schema:CreativeWork
    258 sg:pub.10.1038/nature06196 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050225537
    259 https://doi.org/10.1038/nature06196
    260 rdf:type schema:CreativeWork
    261 sg:pub.10.1038/nbt1310 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024578139
    262 https://doi.org/10.1038/nbt1310
    263 rdf:type schema:CreativeWork
    264 sg:pub.10.1186/1471-213x-1-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042905963
    265 https://doi.org/10.1186/1471-213x-1-4
    266 rdf:type schema:CreativeWork
    267 https://app.dimensions.ai/details/publication/pub.1075305875 schema:CreativeWork
    268 https://app.dimensions.ai/details/publication/pub.1077003237 schema:CreativeWork
    269 https://doi.org/10.1002/aja.1001410403 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045687593
    270 rdf:type schema:CreativeWork
    271 https://doi.org/10.1006/excr.1996.0145 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001447364
    272 rdf:type schema:CreativeWork
    273 https://doi.org/10.1006/excr.2002.5499 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050399692
    274 rdf:type schema:CreativeWork
    275 https://doi.org/10.1016/s0016-5085(99)70344-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044163755
    276 rdf:type schema:CreativeWork
    277 https://doi.org/10.1016/s0076-6879(06)19014-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1016186813
    278 rdf:type schema:CreativeWork
    279 https://doi.org/10.1016/s0092-8674(02)01015-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018479938
    280 rdf:type schema:CreativeWork
    281 https://doi.org/10.1016/s1074-7613(00)80457-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003664648
    282 rdf:type schema:CreativeWork
    283 https://doi.org/10.1053/j.gastro.2006.11.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006367821
    284 rdf:type schema:CreativeWork
    285 https://doi.org/10.1073/pnas.2536800100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050279034
    286 rdf:type schema:CreativeWork
    287 https://doi.org/10.1097/00042737-200107000-00002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003155995
    288 rdf:type schema:CreativeWork
    289 https://doi.org/10.1101/gad.1674008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011867533
    290 rdf:type schema:CreativeWork
    291 https://doi.org/10.1101/gad.267103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011480346
    292 rdf:type schema:CreativeWork
    293 https://doi.org/10.1111/j.1365-2184.1985.tb00655.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1021506214
    294 rdf:type schema:CreativeWork
    295 https://doi.org/10.1111/j.1749-6632.1998.tb11107.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1008007276
    296 rdf:type schema:CreativeWork
    297 https://doi.org/10.1126/science.1093587 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014414968
    298 rdf:type schema:CreativeWork
    299 https://doi.org/10.1126/science.1112521 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050697196
    300 rdf:type schema:CreativeWork
    301 https://doi.org/10.1146/annurev.cellbio.21.012704.131525 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047691540
    302 rdf:type schema:CreativeWork
    303 https://doi.org/10.1152/ajpcell.1999.277.2.c183 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074506095
    304 rdf:type schema:CreativeWork
    305 https://www.grid.ac/institutes/grid.419927.0 schema:alternateName Hubrecht Institute
    306 schema:name Hubrecht Institute and University Medical Center Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands
    307 rdf:type schema:Organization
    308 https://www.grid.ac/institutes/grid.430814.a schema:alternateName Antoni van Leeuwenhoek Hospital
    309 schema:name The Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    310 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...