Single Lgr5 stem cells build crypt–villus structures in vitro without a mesenchymal niche View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-05

AUTHORS

Toshiro Sato, Robert G. Vries, Hugo J. Snippert, Marc van de Wetering, Nick Barker, Daniel E. Stange, Johan H. van Es, Arie Abo, Pekka Kujala, Peter J. Peters, Hans Clevers

ABSTRACT

The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently demonstrated the presence of about six cycling Lgr5(+) stem cells at the bottoms of small-intestinal crypts. Here we describe the establishment of long-term culture conditions under which single crypts undergo multiple crypt fission events, while simultanously generating villus-like epithelial domains in which all differentiated cell types are present. Single sorted Lgr5(+) stem cells can also initiate these cryptvillus organoids. Tracing experiments indicate that the Lgr5(+) stem-cell hierarchy is maintained in organoids. We conclude that intestinal cryptvillus units are self-organizing structures, which can be built from a single stem cell in the absence of a non-epithelial cellular niche. More... »

PAGES

262

Journal

TITLE

Nature

ISSUE

7244

VOLUME

459

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nature07935

    DOI

    http://dx.doi.org/10.1038/nature07935

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1024082069

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/19329995


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