piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2009-04

AUTHORS

Knut Woltjen, Iacovos P. Michael, Paria Mohseni, Ridham Desai, Maria Mileikovsky, Riikka Hämäläinen, Rebecca Cowling, Wei Wang, Pentao Liu, Marina Gertsenstein, Keisuke Kaji, Hoon-Ki Sung, Andras Nagy

ABSTRACT

Transgenic expression of just four defined transcription factors (c-Myc, Klf4, Oct4 and Sox2) is sufficient to reprogram somatic cells to a pluripotent state. The resulting induced pluripotent stem (iPS) cells resemble embryonic stem cells in their properties and potential to differentiate into a spectrum of adult cell types. Current reprogramming strategies involve retroviral, lentiviral, adenoviral and plasmid transfection to deliver reprogramming factor transgenes. Although the latter two methods are transient and minimize the potential for insertion mutagenesis, they are currently limited by diminished reprogramming efficiencies. piggyBac (PB) transposition is host-factor independent, and has recently been demonstrated to be functional in various human and mouse cell lines. The PB transposon/transposase system requires only the inverted terminal repeats flanking a transgene and transient expression of the transposase enzyme to catalyse insertion or excision events. Here we demonstrate successful and efficient reprogramming of murine and human embryonic fibroblasts using doxycycline-inducible transcription factors delivered by PB transposition. Stable iPS cells thus generated express characteristic pluripotency markers and succeed in a series of rigorous differentiation assays. By taking advantage of the natural propensity of the PB system for seamless excision, we show that the individual PB insertions can be removed from established iPS cell lines, providing an invaluable tool for discovery. In addition, we have demonstrated the traceless removal of reprogramming factors joined with viral 2A sequences delivered by a single transposon from murine iPS lines. We anticipate that the unique properties of this virus-independent simplification of iPS cell production will accelerate this field further towards full exploration of the reprogramming process and future cell-based therapies. More... »

PAGES

766

Journal

TITLE

Nature

ISSUE

7239

VOLUME

458

Related Patents

  • Induced Pluripotent Stem Cells Produced With Oct3/4, Klf4 And Sox2
  • Gene Expression System Using Alternative Splicing In Insects
  • Methods And Compositions For Producing Induced Airway Tissue Progenitor Cells
  • Methods And Systems For Converting Precursor Cells Into Gastric Tissues Through Directed Differentiation
  • Reprogramming Cells
  • Biocontrol
  • Methods And Systems For Converting Precursor Cells Into Intestinal Tissues Through Directed Differentiation
  • Cardiomyocytes From Induced Pluripotent Stem Cells From Patients And Methods Of Use Thereof
  • Enriched Population Of Human Pluripotent Cells With Oct-4 And Sox2 Integrated Into Their Genome
  • Protein-Induced Pluripotent Cell Technology And Uses Thereof
  • Rna Preparations Comprising Purified Modified Rna For Reprogramming Cells
  • Expression System For Insect Pest Control
  • Compositions And Methods For Reprogramming Eukaryotic Cells
  • Somatic Cell Reprogramming By Retroviral Vectors Encoding Oct3/4. Klf4, C-Myc And Sox2
  • Cell Compositions Derived From Dedifferentiated Reprogrammed Cells
  • Methods And Platforms For Drug Discovery Using Induced Pluripotent Stem Cells
  • Rna Preparations Comprising Purified Modified Rna For Reprogramming Cells
  • Methods For The Production Of Ips Cells Using Epstein-Barr (Ebv)-Based Reprogramming Vectors
  • Delivery And Formulation Of Engineered Nucleic Acids
  • Rna Preparations Comprising Purified Modified Rna For Reprogramming Cells
  • In Vitro Differentiation Of Pluripotent Stem Cells To Pancreatic Endoderm Cells (Pec) And Endocrine Cells
  • Induced Pluripotent Stem Cells Produced Using Reprogramming Factors And A Rho Kinase Inhibitor Or A Histone Deacetylase Inhibitor
  • Making And Using In Vitro-Synthesized Ssrna For Introducing Into Mammalian Cells To Induce A Biological Or Biochemical Effect
  • Expression Systems
  • Compositions And Methods For Reprogramming Eukaryotic Cells
  • Methods And Compositions For The Rapid Production Of Retinal Pigmented Epithelial Cells From Pluripotent Cells
  • Methods For Maturing Cardiomyocytes And Uses Thereof
  • In Vitro Model Of Spinal Muscular Atrophy
  • Providing Ipscs To A Customer
  • Methods For The Production Of Ips Cells Using Non-Viral Approach
  • Reprogramming Immortalized B-Cells To Induced Pluripotent Stem Cells
  • Generation Of Mouse Induced Pluripotent Stem Cells Bytransient Expression Of A Single Non- Viral Polycistronic Vector
  • Nuclear Reprogramming Factor
  • Methods, Systems And Compositions Relating To Cell Conversion Via Protein-Induced In-Vivo Cell Reprogramming
  • Making And Using In Vitro-Synthesized Ssrna For Introducing Into Mammalian Cells To Induce A Biological Or Biochemical Effect
  • Reprogramming Cells
  • Methods For The Production Of Ips Cells
  • Generation Of Mouse Induced Pluripotent Stem Cells Bytransient Expression Of A Single Non- Viral Polycistronic Vector
  • Oct3/4, Klf4, C-Myc And Sox2 Produce Induced Pluripotent Stem Cells
  • Cell Culture Platform For Single Cell Sorting And Enhanced Reprogramming Of Ipscs
  • Expression Systems
  • Cell Compositions Derived From Dedifferentiated Reprogrammed Cells
  • Cell Compositions Derived From Dedifferentiated Reprogrammed Cells
  • Human Pluripotent Stem Cells Induced From Undifferentiated Stem Cells Derived From A Human Postnatal Tissue
  • Rna Preparations Comprising Purified Modified Rna For Reprogramming Cells
  • Rna Preparations Comprising Purified Modified Rna For Reprogramming Cells
  • Functionalized Nanoparticles For Intracellular Delivery Of Biologically Active Molecules
  • Rna Preparations Comprising Purified Modified Rna For Reprogramming Cells
  • Biological Control
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nature07863

    DOI

    http://dx.doi.org/10.1038/nature07863

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1019816783

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/19252478


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Differentiation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Line", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cells, Cultured", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cellular Reprogramming", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA Transposable Elements", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Fibroblasts", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Order", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Transfer Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Vectors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice, Nude", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Pluripotent Stem Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sequence Alignment", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transcription Factors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transgenes", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Lunenfeld-Tanenbaum Research Institute", 
              "id": "https://www.grid.ac/institutes/grid.250674.2", 
              "name": [
                "Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Woltjen", 
            "givenName": "Knut", 
            "id": "sg:person.01123666274.06", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123666274.06"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Toronto", 
              "id": "https://www.grid.ac/institutes/grid.17063.33", 
              "name": [
                "Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada", 
                "Department of Molecular Genetics, University of Toronto, Toronto M5S 1A8, Canada"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Michael", 
            "givenName": "Iacovos P.", 
            "id": "sg:person.01172001474.37", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01172001474.37"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Toronto", 
              "id": "https://www.grid.ac/institutes/grid.17063.33", 
              "name": [
                "Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada", 
                "Department of Molecular Genetics, University of Toronto, Toronto M5S 1A8, Canada"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mohseni", 
            "givenName": "Paria", 
            "id": "sg:person.01007437674.97", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01007437674.97"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Toronto", 
              "id": "https://www.grid.ac/institutes/grid.17063.33", 
              "name": [
                "Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada", 
                "Department of Molecular Genetics, University of Toronto, Toronto M5S 1A8, Canada"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Desai", 
            "givenName": "Ridham", 
            "id": "sg:person.01306230074.68", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01306230074.68"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Lunenfeld-Tanenbaum Research Institute", 
              "id": "https://www.grid.ac/institutes/grid.250674.2", 
              "name": [
                "Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mileikovsky", 
            "givenName": "Maria", 
            "id": "sg:person.0661735626.03", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0661735626.03"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Lunenfeld-Tanenbaum Research Institute", 
              "id": "https://www.grid.ac/institutes/grid.250674.2", 
              "name": [
                "Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada"
              ], 
              "type": "Organization"
            }, 
            "familyName": "H\u00e4m\u00e4l\u00e4inen", 
            "givenName": "Riikka", 
            "id": "sg:person.01222706623.75", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01222706623.75"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Lunenfeld-Tanenbaum Research Institute", 
              "id": "https://www.grid.ac/institutes/grid.250674.2", 
              "name": [
                "Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Cowling", 
            "givenName": "Rebecca", 
            "id": "sg:person.01123573677.73", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123573677.73"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wellcome Sanger Institute", 
              "id": "https://www.grid.ac/institutes/grid.10306.34", 
              "name": [
                "The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wang", 
            "givenName": "Wei", 
            "id": "sg:person.0736732221.30", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0736732221.30"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Wellcome Sanger Institute", 
              "id": "https://www.grid.ac/institutes/grid.10306.34", 
              "name": [
                "The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Liu", 
            "givenName": "Pentao", 
            "id": "sg:person.011706755557.44", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011706755557.44"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Lunenfeld-Tanenbaum Research Institute", 
              "id": "https://www.grid.ac/institutes/grid.250674.2", 
              "name": [
                "Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gertsenstein", 
            "givenName": "Marina", 
            "id": "sg:person.0600752164.08", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0600752164.08"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "MRC Centre for Regenerative Medicine", 
              "id": "https://www.grid.ac/institutes/grid.483689.8", 
              "name": [
                "MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, University of Edinburgh, Edinburgh EH9 3JQ, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kaji", 
            "givenName": "Keisuke", 
            "id": "sg:person.01350502174.86", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01350502174.86"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Lunenfeld-Tanenbaum Research Institute", 
              "id": "https://www.grid.ac/institutes/grid.250674.2", 
              "name": [
                "Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sung", 
            "givenName": "Hoon-Ki", 
            "id": "sg:person.01331133070.20", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01331133070.20"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Toronto", 
              "id": "https://www.grid.ac/institutes/grid.17063.33", 
              "name": [
                "Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada", 
                "Department of Molecular Genetics, University of Toronto, Toronto M5S 1A8, Canada"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nagy", 
            "givenName": "Andras", 
            "id": "sg:person.0703776201.27", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0703776201.27"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/nature07864", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002136274", 
              "https://doi.org/10.1038/nature07864"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature05934", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002347899", 
              "https://doi.org/10.1038/nature05934"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nprot.2007.418", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003600573", 
              "https://doi.org/10.1038/nprot.2007.418"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1483", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004160248", 
              "https://doi.org/10.1038/nbt1483"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0801017105", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006009044"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/jgm.566", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006527442"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2008.02.001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008490443"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncb1372", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009099013", 
              "https://doi.org/10.1038/ncb1372"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ncb1372", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009099013", 
              "https://doi.org/10.1038/ncb1372"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2006.07.024", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014573758"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature07061", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017686107", 
              "https://doi.org/10.1038/nature07061"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1221", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019022311", 
              "https://doi.org/10.1038/nbt1221"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1221", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019022311", 
              "https://doi.org/10.1038/nbt1221"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1038/emboj.2008.41", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020644418"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.0606979103", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020814347"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1162494", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021871278"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.90.18.8424", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023550877"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gni051", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027796295"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2007.05.014", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029649252"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-2583.1996.tb00048.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031210115"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1038/sj.mt.6300028", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033716290"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0042-6822(89)90117-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033876784"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature07056", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037743725", 
              "https://doi.org/10.1038/nature07056"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.cell.2005.07.013", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039880587"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.91.25.12258", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042614837"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkm446", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045124061"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt1335", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049953935", 
              "https://doi.org/10.1038/nbt1335"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt.1502", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051466228", 
              "https://doi.org/10.1038/nbt.1502"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.stem.2008.01.004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053248333"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1089/hum.2005.16.1241", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1059272465"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1164270", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062458687"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2009-04", 
        "datePublishedReg": "2009-04-01", 
        "description": "Transgenic expression of just four defined transcription factors (c-Myc, Klf4, Oct4 and Sox2) is sufficient to reprogram somatic cells to a pluripotent state. The resulting induced pluripotent stem (iPS) cells resemble embryonic stem cells in their properties and potential to differentiate into a spectrum of adult cell types. Current reprogramming strategies involve retroviral, lentiviral, adenoviral and plasmid transfection to deliver reprogramming factor transgenes. Although the latter two methods are transient and minimize the potential for insertion mutagenesis, they are currently limited by diminished reprogramming efficiencies. piggyBac (PB) transposition is host-factor independent, and has recently been demonstrated to be functional in various human and mouse cell lines. The PB transposon/transposase system requires only the inverted terminal repeats flanking a transgene and transient expression of the transposase enzyme to catalyse insertion or excision events. Here we demonstrate successful and efficient reprogramming of murine and human embryonic fibroblasts using doxycycline-inducible transcription factors delivered by PB transposition. Stable iPS cells thus generated express characteristic pluripotency markers and succeed in a series of rigorous differentiation assays. By taking advantage of the natural propensity of the PB system for seamless excision, we show that the individual PB insertions can be removed from established iPS cell lines, providing an invaluable tool for discovery. In addition, we have demonstrated the traceless removal of reprogramming factors joined with viral 2A sequences delivered by a single transposon from murine iPS lines. We anticipate that the unique properties of this virus-independent simplification of iPS cell production will accelerate this field further towards full exploration of the reprogramming process and future cell-based therapies.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nature07863", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2758863", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1018957", 
            "issn": [
              "0090-0028", 
              "1476-4687"
            ], 
            "name": "Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "7239", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "458"
          }
        ], 
        "name": "piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells", 
        "pagination": "766", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "5e6b1cc604ca35c2085d5f09c34aaf2efc9685696c76c26de51aa722e7248776"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "19252478"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "0410462"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nature07863"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1019816783"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nature07863", 
          "https://app.dimensions.ai/details/publication/pub.1019816783"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T21:24", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8687_00000424.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://www.nature.com/articles/nature07863"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature07863'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature07863'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature07863'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature07863'


     

    This table displays all metadata directly associated to this object as RDF triples.

    330 TRIPLES      21 PREDICATES      75 URIs      38 LITERALS      26 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nature07863 schema:about N039ab681010e40a5ad2ea32a25abbf6c
    2 N413817ea276143b68e34677e1a9545b6
    3 N49cc7ff4c23741a69717ddd5dc29d4d7
    4 N4c33d5d587b44d19934f1f7efa640d64
    5 N508610d90a8a4c368484d3a641c3591e
    6 N5c72d78c2a2a4e20a02788ae8f878e32
    7 N6602f56a5b1442a6a75ff90e3193af11
    8 N7396df57ae324c5f82063a27eba64413
    9 N76df988db8fd438ca6337c7303de87c6
    10 N7d0f465fef5e4ca780fdf8108d78ccbb
    11 N9aacaea56dc24e408abebf740bfc83ed
    12 Nad41ab1dd5c444e09a3dae7cbe42d3b6
    13 Nb37c4e3d95074cbe8869b0f644354066
    14 Nb497614051e34df196165d11a49648a2
    15 Nc01e1a22ca2c4196950f98ad3b62b3af
    16 Nd4cb8e5f8fbe4d28a390f0116646e369
    17 Nd6b496400b74454a9a9f06a6456f294a
    18 anzsrc-for:06
    19 anzsrc-for:0601
    20 schema:author N9e65282151be4c7a8db9dfb57ff33e83
    21 schema:citation sg:pub.10.1038/nature05934
    22 sg:pub.10.1038/nature07056
    23 sg:pub.10.1038/nature07061
    24 sg:pub.10.1038/nature07864
    25 sg:pub.10.1038/nbt.1502
    26 sg:pub.10.1038/nbt1221
    27 sg:pub.10.1038/nbt1335
    28 sg:pub.10.1038/nbt1483
    29 sg:pub.10.1038/ncb1372
    30 sg:pub.10.1038/nprot.2007.418
    31 https://doi.org/10.1002/jgm.566
    32 https://doi.org/10.1016/0042-6822(89)90117-7
    33 https://doi.org/10.1016/j.cell.2005.07.013
    34 https://doi.org/10.1016/j.cell.2006.07.024
    35 https://doi.org/10.1016/j.stem.2007.05.014
    36 https://doi.org/10.1016/j.stem.2008.01.004
    37 https://doi.org/10.1016/j.stem.2008.02.001
    38 https://doi.org/10.1038/emboj.2008.41
    39 https://doi.org/10.1038/sj.mt.6300028
    40 https://doi.org/10.1073/pnas.0606979103
    41 https://doi.org/10.1073/pnas.0801017105
    42 https://doi.org/10.1073/pnas.90.18.8424
    43 https://doi.org/10.1073/pnas.91.25.12258
    44 https://doi.org/10.1089/hum.2005.16.1241
    45 https://doi.org/10.1093/nar/gkm446
    46 https://doi.org/10.1093/nar/gni051
    47 https://doi.org/10.1111/j.1365-2583.1996.tb00048.x
    48 https://doi.org/10.1126/science.1162494
    49 https://doi.org/10.1126/science.1164270
    50 schema:datePublished 2009-04
    51 schema:datePublishedReg 2009-04-01
    52 schema:description Transgenic expression of just four defined transcription factors (c-Myc, Klf4, Oct4 and Sox2) is sufficient to reprogram somatic cells to a pluripotent state. The resulting induced pluripotent stem (iPS) cells resemble embryonic stem cells in their properties and potential to differentiate into a spectrum of adult cell types. Current reprogramming strategies involve retroviral, lentiviral, adenoviral and plasmid transfection to deliver reprogramming factor transgenes. Although the latter two methods are transient and minimize the potential for insertion mutagenesis, they are currently limited by diminished reprogramming efficiencies. piggyBac (PB) transposition is host-factor independent, and has recently been demonstrated to be functional in various human and mouse cell lines. The PB transposon/transposase system requires only the inverted terminal repeats flanking a transgene and transient expression of the transposase enzyme to catalyse insertion or excision events. Here we demonstrate successful and efficient reprogramming of murine and human embryonic fibroblasts using doxycycline-inducible transcription factors delivered by PB transposition. Stable iPS cells thus generated express characteristic pluripotency markers and succeed in a series of rigorous differentiation assays. By taking advantage of the natural propensity of the PB system for seamless excision, we show that the individual PB insertions can be removed from established iPS cell lines, providing an invaluable tool for discovery. In addition, we have demonstrated the traceless removal of reprogramming factors joined with viral 2A sequences delivered by a single transposon from murine iPS lines. We anticipate that the unique properties of this virus-independent simplification of iPS cell production will accelerate this field further towards full exploration of the reprogramming process and future cell-based therapies.
    53 schema:genre research_article
    54 schema:inLanguage en
    55 schema:isAccessibleForFree true
    56 schema:isPartOf N291bffa6d61d4601bc6913ef6e4e648e
    57 Na629a481a4494d859bdfaee3337baa4a
    58 sg:journal.1018957
    59 schema:name piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells
    60 schema:pagination 766
    61 schema:productId N04500679e6034e549c2e7915429c2fd2
    62 N0adb94eccd964d858410c3406bacab33
    63 N2e4c145038e941c983ede8dd9c83cd98
    64 N4afa3e78572d4ea6bf041941861015d6
    65 N72e35615f09b4f85bcb626bef344c678
    66 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019816783
    67 https://doi.org/10.1038/nature07863
    68 schema:sdDatePublished 2019-04-10T21:24
    69 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    70 schema:sdPublisher Nf71c4850c87943e88bc493d5b02a1948
    71 schema:url https://www.nature.com/articles/nature07863
    72 sgo:license sg:explorer/license/
    73 sgo:sdDataset articles
    74 rdf:type schema:ScholarlyArticle
    75 N039ab681010e40a5ad2ea32a25abbf6c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    76 schema:name Mice, Nude
    77 rdf:type schema:DefinedTerm
    78 N04500679e6034e549c2e7915429c2fd2 schema:name dimensions_id
    79 schema:value pub.1019816783
    80 rdf:type schema:PropertyValue
    81 N0adb94eccd964d858410c3406bacab33 schema:name readcube_id
    82 schema:value 5e6b1cc604ca35c2085d5f09c34aaf2efc9685696c76c26de51aa722e7248776
    83 rdf:type schema:PropertyValue
    84 N1225b9a620114670b0e960de64a3122e rdf:first sg:person.011706755557.44
    85 rdf:rest Ne8d411c54c254f818cab7195f590926c
    86 N1d94e46be0054a26bbd26d06fd6b69bb rdf:first sg:person.01350502174.86
    87 rdf:rest Nf26fcdf72fe34a6087fa66e9675e9bb4
    88 N2472791f039b4f5eb967e1ed65e78833 rdf:first sg:person.01172001474.37
    89 rdf:rest N2bf384a354f2418ab72bb6b2210b231c
    90 N291bffa6d61d4601bc6913ef6e4e648e schema:issueNumber 7239
    91 rdf:type schema:PublicationIssue
    92 N2bf384a354f2418ab72bb6b2210b231c rdf:first sg:person.01007437674.97
    93 rdf:rest Na79b5f2d98fe42188d05243a86532076
    94 N2e4c145038e941c983ede8dd9c83cd98 schema:name nlm_unique_id
    95 schema:value 0410462
    96 rdf:type schema:PropertyValue
    97 N413817ea276143b68e34677e1a9545b6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    98 schema:name Cell Line
    99 rdf:type schema:DefinedTerm
    100 N49cc7ff4c23741a69717ddd5dc29d4d7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    101 schema:name Transcription Factors
    102 rdf:type schema:DefinedTerm
    103 N4afa3e78572d4ea6bf041941861015d6 schema:name doi
    104 schema:value 10.1038/nature07863
    105 rdf:type schema:PropertyValue
    106 N4c33d5d587b44d19934f1f7efa640d64 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    107 schema:name Sequence Alignment
    108 rdf:type schema:DefinedTerm
    109 N508610d90a8a4c368484d3a641c3591e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name Genetic Vectors
    111 rdf:type schema:DefinedTerm
    112 N5b23063aceb0427da7ceaa775c81c27b rdf:first sg:person.0703776201.27
    113 rdf:rest rdf:nil
    114 N5c72d78c2a2a4e20a02788ae8f878e32 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    115 schema:name Fibroblasts
    116 rdf:type schema:DefinedTerm
    117 N6602f56a5b1442a6a75ff90e3193af11 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    118 schema:name Mice
    119 rdf:type schema:DefinedTerm
    120 N72e35615f09b4f85bcb626bef344c678 schema:name pubmed_id
    121 schema:value 19252478
    122 rdf:type schema:PropertyValue
    123 N7396df57ae324c5f82063a27eba64413 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    124 schema:name Humans
    125 rdf:type schema:DefinedTerm
    126 N76df988db8fd438ca6337c7303de87c6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name Gene Order
    128 rdf:type schema:DefinedTerm
    129 N7d0f465fef5e4ca780fdf8108d78ccbb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    130 schema:name Pluripotent Stem Cells
    131 rdf:type schema:DefinedTerm
    132 N9aacaea56dc24e408abebf740bfc83ed schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    133 schema:name Cells, Cultured
    134 rdf:type schema:DefinedTerm
    135 N9e65282151be4c7a8db9dfb57ff33e83 rdf:first sg:person.01123666274.06
    136 rdf:rest N2472791f039b4f5eb967e1ed65e78833
    137 Na629a481a4494d859bdfaee3337baa4a schema:volumeNumber 458
    138 rdf:type schema:PublicationVolume
    139 Na79b5f2d98fe42188d05243a86532076 rdf:first sg:person.01306230074.68
    140 rdf:rest Nf09e2a52e8304710a00abfe7960e7d89
    141 Nad41ab1dd5c444e09a3dae7cbe42d3b6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    142 schema:name Animals
    143 rdf:type schema:DefinedTerm
    144 Naff516ca24e247f298ad9ac2b2d3be57 rdf:first sg:person.0736732221.30
    145 rdf:rest N1225b9a620114670b0e960de64a3122e
    146 Nb37c4e3d95074cbe8869b0f644354066 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    147 schema:name Transgenes
    148 rdf:type schema:DefinedTerm
    149 Nb497614051e34df196165d11a49648a2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    150 schema:name Cell Differentiation
    151 rdf:type schema:DefinedTerm
    152 Nc01e1a22ca2c4196950f98ad3b62b3af schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    153 schema:name Cellular Reprogramming
    154 rdf:type schema:DefinedTerm
    155 Nd4cb8e5f8fbe4d28a390f0116646e369 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    156 schema:name DNA Transposable Elements
    157 rdf:type schema:DefinedTerm
    158 Nd6b496400b74454a9a9f06a6456f294a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    159 schema:name Gene Transfer Techniques
    160 rdf:type schema:DefinedTerm
    161 Ndfcb67ba3b04490cbb349a514bf035e2 rdf:first sg:person.01222706623.75
    162 rdf:rest Ne2554e12cef74bfb8629ac2b490c45f1
    163 Ne2554e12cef74bfb8629ac2b490c45f1 rdf:first sg:person.01123573677.73
    164 rdf:rest Naff516ca24e247f298ad9ac2b2d3be57
    165 Ne8d411c54c254f818cab7195f590926c rdf:first sg:person.0600752164.08
    166 rdf:rest N1d94e46be0054a26bbd26d06fd6b69bb
    167 Nf09e2a52e8304710a00abfe7960e7d89 rdf:first sg:person.0661735626.03
    168 rdf:rest Ndfcb67ba3b04490cbb349a514bf035e2
    169 Nf26fcdf72fe34a6087fa66e9675e9bb4 rdf:first sg:person.01331133070.20
    170 rdf:rest N5b23063aceb0427da7ceaa775c81c27b
    171 Nf71c4850c87943e88bc493d5b02a1948 schema:name Springer Nature - SN SciGraph project
    172 rdf:type schema:Organization
    173 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    174 schema:name Biological Sciences
    175 rdf:type schema:DefinedTerm
    176 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    177 schema:name Biochemistry and Cell Biology
    178 rdf:type schema:DefinedTerm
    179 sg:grant.2758863 http://pending.schema.org/fundedItem sg:pub.10.1038/nature07863
    180 rdf:type schema:MonetaryGrant
    181 sg:journal.1018957 schema:issn 0090-0028
    182 1476-4687
    183 schema:name Nature
    184 rdf:type schema:Periodical
    185 sg:person.01007437674.97 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
    186 schema:familyName Mohseni
    187 schema:givenName Paria
    188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01007437674.97
    189 rdf:type schema:Person
    190 sg:person.01123573677.73 schema:affiliation https://www.grid.ac/institutes/grid.250674.2
    191 schema:familyName Cowling
    192 schema:givenName Rebecca
    193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123573677.73
    194 rdf:type schema:Person
    195 sg:person.01123666274.06 schema:affiliation https://www.grid.ac/institutes/grid.250674.2
    196 schema:familyName Woltjen
    197 schema:givenName Knut
    198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123666274.06
    199 rdf:type schema:Person
    200 sg:person.011706755557.44 schema:affiliation https://www.grid.ac/institutes/grid.10306.34
    201 schema:familyName Liu
    202 schema:givenName Pentao
    203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011706755557.44
    204 rdf:type schema:Person
    205 sg:person.01172001474.37 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
    206 schema:familyName Michael
    207 schema:givenName Iacovos P.
    208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01172001474.37
    209 rdf:type schema:Person
    210 sg:person.01222706623.75 schema:affiliation https://www.grid.ac/institutes/grid.250674.2
    211 schema:familyName Hämäläinen
    212 schema:givenName Riikka
    213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01222706623.75
    214 rdf:type schema:Person
    215 sg:person.01306230074.68 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
    216 schema:familyName Desai
    217 schema:givenName Ridham
    218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01306230074.68
    219 rdf:type schema:Person
    220 sg:person.01331133070.20 schema:affiliation https://www.grid.ac/institutes/grid.250674.2
    221 schema:familyName Sung
    222 schema:givenName Hoon-Ki
    223 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01331133070.20
    224 rdf:type schema:Person
    225 sg:person.01350502174.86 schema:affiliation https://www.grid.ac/institutes/grid.483689.8
    226 schema:familyName Kaji
    227 schema:givenName Keisuke
    228 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01350502174.86
    229 rdf:type schema:Person
    230 sg:person.0600752164.08 schema:affiliation https://www.grid.ac/institutes/grid.250674.2
    231 schema:familyName Gertsenstein
    232 schema:givenName Marina
    233 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0600752164.08
    234 rdf:type schema:Person
    235 sg:person.0661735626.03 schema:affiliation https://www.grid.ac/institutes/grid.250674.2
    236 schema:familyName Mileikovsky
    237 schema:givenName Maria
    238 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0661735626.03
    239 rdf:type schema:Person
    240 sg:person.0703776201.27 schema:affiliation https://www.grid.ac/institutes/grid.17063.33
    241 schema:familyName Nagy
    242 schema:givenName Andras
    243 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0703776201.27
    244 rdf:type schema:Person
    245 sg:person.0736732221.30 schema:affiliation https://www.grid.ac/institutes/grid.10306.34
    246 schema:familyName Wang
    247 schema:givenName Wei
    248 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0736732221.30
    249 rdf:type schema:Person
    250 sg:pub.10.1038/nature05934 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002347899
    251 https://doi.org/10.1038/nature05934
    252 rdf:type schema:CreativeWork
    253 sg:pub.10.1038/nature07056 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037743725
    254 https://doi.org/10.1038/nature07056
    255 rdf:type schema:CreativeWork
    256 sg:pub.10.1038/nature07061 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017686107
    257 https://doi.org/10.1038/nature07061
    258 rdf:type schema:CreativeWork
    259 sg:pub.10.1038/nature07864 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002136274
    260 https://doi.org/10.1038/nature07864
    261 rdf:type schema:CreativeWork
    262 sg:pub.10.1038/nbt.1502 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051466228
    263 https://doi.org/10.1038/nbt.1502
    264 rdf:type schema:CreativeWork
    265 sg:pub.10.1038/nbt1221 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019022311
    266 https://doi.org/10.1038/nbt1221
    267 rdf:type schema:CreativeWork
    268 sg:pub.10.1038/nbt1335 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049953935
    269 https://doi.org/10.1038/nbt1335
    270 rdf:type schema:CreativeWork
    271 sg:pub.10.1038/nbt1483 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004160248
    272 https://doi.org/10.1038/nbt1483
    273 rdf:type schema:CreativeWork
    274 sg:pub.10.1038/ncb1372 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009099013
    275 https://doi.org/10.1038/ncb1372
    276 rdf:type schema:CreativeWork
    277 sg:pub.10.1038/nprot.2007.418 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003600573
    278 https://doi.org/10.1038/nprot.2007.418
    279 rdf:type schema:CreativeWork
    280 https://doi.org/10.1002/jgm.566 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006527442
    281 rdf:type schema:CreativeWork
    282 https://doi.org/10.1016/0042-6822(89)90117-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033876784
    283 rdf:type schema:CreativeWork
    284 https://doi.org/10.1016/j.cell.2005.07.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039880587
    285 rdf:type schema:CreativeWork
    286 https://doi.org/10.1016/j.cell.2006.07.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014573758
    287 rdf:type schema:CreativeWork
    288 https://doi.org/10.1016/j.stem.2007.05.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029649252
    289 rdf:type schema:CreativeWork
    290 https://doi.org/10.1016/j.stem.2008.01.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053248333
    291 rdf:type schema:CreativeWork
    292 https://doi.org/10.1016/j.stem.2008.02.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008490443
    293 rdf:type schema:CreativeWork
    294 https://doi.org/10.1038/emboj.2008.41 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020644418
    295 rdf:type schema:CreativeWork
    296 https://doi.org/10.1038/sj.mt.6300028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033716290
    297 rdf:type schema:CreativeWork
    298 https://doi.org/10.1073/pnas.0606979103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020814347
    299 rdf:type schema:CreativeWork
    300 https://doi.org/10.1073/pnas.0801017105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006009044
    301 rdf:type schema:CreativeWork
    302 https://doi.org/10.1073/pnas.90.18.8424 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023550877
    303 rdf:type schema:CreativeWork
    304 https://doi.org/10.1073/pnas.91.25.12258 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042614837
    305 rdf:type schema:CreativeWork
    306 https://doi.org/10.1089/hum.2005.16.1241 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059272465
    307 rdf:type schema:CreativeWork
    308 https://doi.org/10.1093/nar/gkm446 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045124061
    309 rdf:type schema:CreativeWork
    310 https://doi.org/10.1093/nar/gni051 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027796295
    311 rdf:type schema:CreativeWork
    312 https://doi.org/10.1111/j.1365-2583.1996.tb00048.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1031210115
    313 rdf:type schema:CreativeWork
    314 https://doi.org/10.1126/science.1162494 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021871278
    315 rdf:type schema:CreativeWork
    316 https://doi.org/10.1126/science.1164270 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062458687
    317 rdf:type schema:CreativeWork
    318 https://www.grid.ac/institutes/grid.10306.34 schema:alternateName Wellcome Sanger Institute
    319 schema:name The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
    320 rdf:type schema:Organization
    321 https://www.grid.ac/institutes/grid.17063.33 schema:alternateName University of Toronto
    322 schema:name Department of Molecular Genetics, University of Toronto, Toronto M5S 1A8, Canada
    323 Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
    324 rdf:type schema:Organization
    325 https://www.grid.ac/institutes/grid.250674.2 schema:alternateName Lunenfeld-Tanenbaum Research Institute
    326 schema:name Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
    327 rdf:type schema:Organization
    328 https://www.grid.ac/institutes/grid.483689.8 schema:alternateName MRC Centre for Regenerative Medicine
    329 schema:name MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, University of Edinburgh, Edinburgh EH9 3JQ, UK
    330 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...