Ontology type: schema:ScholarlyArticle Open Access: True
2008-11
AUTHORSEric T. Wang, Rickard Sandberg, Shujun Luo, Irina Khrebtukova, Lu Zhang, Christine Mayr, Stephen F. Kingsmore, Gary P. Schroth, Christopher B. Burge
ABSTRACTThrough alternative processing of pre-messenger RNAs, individual mammalian genes often produce multiple mRNA and protein isoforms that may have related, distinct or even opposing functions. Here we report an in-depth analysis of 15 diverse human tissue and cell line transcriptomes on the basis of deep sequencing of complementary DNA fragments, yielding a digital inventory of gene and mRNA isoform expression. Analyses in which sequence reads are mapped to exon-exon junctions indicated that 92-94% of human genes undergo alternative splicing, 86% with a minor isoform frequency of 15% or more. Differences in isoform-specific read densities indicated that most alternative splicing and alternative cleavage and polyadenylation events vary between tissues, whereas variation between individuals was approximately twofold to threefold less common. Extreme or 'switch-like' regulation of splicing between tissues was associated with increased sequence conservation in regulatory regions and with generation of full-length open reading frames. Patterns of alternative splicing and alternative cleavage and polyadenylation were strongly correlated across tissues, suggesting coordinated regulation of these processes, and sequence conservation of a subset of known regulatory motifs in both alternative introns and 3' untranslated regions suggested common involvement of specific factors in tissue-level regulation of both splicing and polyadenylation. More... »
PAGES470
http://scigraph.springernature.com/pub.10.1038/nature07509
DOIhttp://dx.doi.org/10.1038/nature07509
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1029002744
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/18978772
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Alternative Splicing",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Base Sequence",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Line",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Exons",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gene Expression Profiling",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Open Reading Frames",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Organ Specificity",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Polyadenylation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Protein Isoforms",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "RNA Splicing Factors",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "RNA, Messenger",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "RNA-Binding Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Repressor Proteins",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Harvard\u2013MIT Division of Health Sciences and Technology",
"id": "https://www.grid.ac/institutes/grid.413735.7",
"name": [
"Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA",
"Harvard-MIT Division of Health Sciences and Technology, Cambridge, Massachusetts 02139, USA"
],
"type": "Organization"
},
"familyName": "Wang",
"givenName": "Eric T.",
"id": "sg:person.01065166141.50",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065166141.50"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Karolinska Institute",
"id": "https://www.grid.ac/institutes/grid.4714.6",
"name": [
"Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA",
"Department of Cell and Molecular Biology, Karolinska Institutet, 171 77 Stockholm, Sweden"
],
"type": "Organization"
},
"familyName": "Sandberg",
"givenName": "Rickard",
"id": "sg:person.01164777574.83",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01164777574.83"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Illumina (United States)",
"id": "https://www.grid.ac/institutes/grid.185669.5",
"name": [
"Illumina Inc., 25861 Industrial Boulevard, Hayward, California 94545, USA"
],
"type": "Organization"
},
"familyName": "Luo",
"givenName": "Shujun",
"id": "sg:person.01201414541.50",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01201414541.50"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Illumina (United States)",
"id": "https://www.grid.ac/institutes/grid.185669.5",
"name": [
"Illumina Inc., 25861 Industrial Boulevard, Hayward, California 94545, USA"
],
"type": "Organization"
},
"familyName": "Khrebtukova",
"givenName": "Irina",
"id": "sg:person.01141657675.39",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01141657675.39"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Illumina (United States)",
"id": "https://www.grid.ac/institutes/grid.185669.5",
"name": [
"Illumina Inc., 25861 Industrial Boulevard, Hayward, California 94545, USA"
],
"type": "Organization"
},
"familyName": "Zhang",
"givenName": "Lu",
"id": "sg:person.01315643141.71",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315643141.71"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Massachusetts Institute of Technology",
"id": "https://www.grid.ac/institutes/grid.116068.8",
"name": [
"Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA"
],
"type": "Organization"
},
"familyName": "Mayr",
"givenName": "Christine",
"id": "sg:person.01252533300.86",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01252533300.86"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "National Center for Genome Resources",
"id": "https://www.grid.ac/institutes/grid.419253.8",
"name": [
"National Center for Genome Resources, 2935 Rodeo Park Drive East, Santa Fe, New Mexico 87505, USA"
],
"type": "Organization"
},
"familyName": "Kingsmore",
"givenName": "Stephen F.",
"id": "sg:person.0617554644.04",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617554644.04"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Illumina (United States)",
"id": "https://www.grid.ac/institutes/grid.185669.5",
"name": [
"Illumina Inc., 25861 Industrial Boulevard, Hayward, California 94545, USA"
],
"type": "Organization"
},
"familyName": "Schroth",
"givenName": "Gary P.",
"id": "sg:person.01314652476.77",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01314652476.77"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Massachusetts Institute of Technology",
"id": "https://www.grid.ac/institutes/grid.116068.8",
"name": [
"Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA"
],
"type": "Organization"
},
"familyName": "Burge",
"givenName": "Christopher B.",
"id": "sg:person.01346557504.20",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01346557504.20"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1016/j.molcel.2004.12.004",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000823839"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pgen.0010034",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001093944"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pgen.0010034",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001093944"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/1471-2164-8-152",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002634676",
"https://doi.org/10.1186/1471-2164-8-152"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.tig.2007.10.004",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004554699"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/gad.828901",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004835689"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrm1645",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006009626",
"https://doi.org/10.1038/nrm1645"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrm1645",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006009626",
"https://doi.org/10.1038/nrm1645"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nrm1645",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006009626",
"https://doi.org/10.1038/nrm1645"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/1471-2164-5-72",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1008202912",
"https://doi.org/10.1186/1471-2164-5-72"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/gki338",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1008699746"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.1090100",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009574243"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.072090399",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009718657"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1074/jbc.m802803200",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010098413"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1038/sj.emboj.7600918",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010160876"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1038/sj.emboj.7600918",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010160876"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/mcb.22.1.148-160.2002",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1011010976"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.cell.2006.06.023",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013282821"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.0136770100",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018919463"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.cell.2004.12.035",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020132624"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nsmb963",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021800877",
"https://doi.org/10.1038/nsmb963"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nsmb963",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021800877",
"https://doi.org/10.1038/nsmb963"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nsmb963",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021800877",
"https://doi.org/10.1038/nsmb963"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.febslet.2004.10.018",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025719627"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/gb-2002-3-11-reviews0008",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027554256",
"https://doi.org/10.1186/gb-2002-3-11-reviews0008"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature05304",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027883190",
"https://doi.org/10.1038/nature05304"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature05304",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027883190",
"https://doi.org/10.1038/nature05304"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature05304",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027883190",
"https://doi.org/10.1038/nature05304"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/416499a",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030329468",
"https://doi.org/10.1038/416499a"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/416499a",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030329468",
"https://doi.org/10.1038/416499a"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/mcb.24.10.4174-4183.2004",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030654404"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1239",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037875102",
"https://doi.org/10.1038/nbt1239"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1239",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037875102",
"https://doi.org/10.1038/nbt1239"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/gkf492",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1041173262"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.molcel.2006.05.018",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1041494953"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.1160342",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042163407"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/35057062",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042854081",
"https://doi.org/10.1038/35057062"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/35057062",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042854081",
"https://doi.org/10.1038/35057062"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ng.2007.57",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043566648",
"https://doi.org/10.1038/ng.2007.57"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/gb-2004-5-10-r74",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044654895",
"https://doi.org/10.1186/gb-2004-5-10-r74"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.molcel.2008.01.005",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045011034"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nmeth.1226",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045381177",
"https://doi.org/10.1038/nmeth.1226"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1146/annurev.biochem.72.121801.161720",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045905529"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/mcb.25.22.10005-10016.2005",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046767346"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ng.259",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050283464",
"https://doi.org/10.1038/ng.259"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature03441",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050742226",
"https://doi.org/10.1038/nature03441"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature03441",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050742226",
"https://doi.org/10.1038/nature03441"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1371/journal.pcbi.0020004",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050910069"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1086/511788",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1058788107"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.1114066",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062452326"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1176/ajp.2006.163.10.1829",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1063486226"
],
"type": "CreativeWork"
}
],
"datePublished": "2008-11",
"datePublishedReg": "2008-11-01",
"description": "Through alternative processing of pre-messenger RNAs, individual mammalian genes often produce multiple mRNA and protein isoforms that may have related, distinct or even opposing functions. Here we report an in-depth analysis of 15 diverse human tissue and cell line transcriptomes on the basis of deep sequencing of complementary DNA fragments, yielding a digital inventory of gene and mRNA isoform expression. Analyses in which sequence reads are mapped to exon-exon junctions indicated that 92-94% of human genes undergo alternative splicing, 86% with a minor isoform frequency of 15% or more. Differences in isoform-specific read densities indicated that most alternative splicing and alternative cleavage and polyadenylation events vary between tissues, whereas variation between individuals was approximately twofold to threefold less common. Extreme or 'switch-like' regulation of splicing between tissues was associated with increased sequence conservation in regulatory regions and with generation of full-length open reading frames. Patterns of alternative splicing and alternative cleavage and polyadenylation were strongly correlated across tissues, suggesting coordinated regulation of these processes, and sequence conservation of a subset of known regulatory motifs in both alternative introns and 3' untranslated regions suggested common involvement of specific factors in tissue-level regulation of both splicing and polyadenylation.",
"genre": "research_article",
"id": "sg:pub.10.1038/nature07509",
"inLanguage": [
"en"
],
"isAccessibleForFree": true,
"isFundedItemOf": [
{
"id": "sg:grant.2529117",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.2520165",
"type": "MonetaryGrant"
}
],
"isPartOf": [
{
"id": "sg:journal.1018957",
"issn": [
"0090-0028",
"1476-4687"
],
"name": "Nature",
"type": "Periodical"
},
{
"issueNumber": "7221",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "456"
}
],
"name": "Alternative isoform regulation in human tissue transcriptomes",
"pagination": "470",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"8a29abb1b72e71e2a5d71b11d28379779466868e53bf608f3980b5bc143765dc"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"18978772"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"0410462"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/nature07509"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1029002744"
]
}
],
"sameAs": [
"https://doi.org/10.1038/nature07509",
"https://app.dimensions.ai/details/publication/pub.1029002744"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-10T21:24",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8687_00000425.jsonl",
"type": "ScholarlyArticle",
"url": "https://www.nature.com/articles/nature07509"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature07509'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature07509'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature07509'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature07509'
This table displays all metadata directly associated to this object as RDF triples.
331 TRIPLES
21 PREDICATES
82 URIs
35 LITERALS
23 BLANK NODES