The pathogen protein EspFU hijacks actin polymerization using mimicry and multivalency View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2008-07-23

AUTHORS

Nathan A. Sallee, Gonzalo M. Rivera, John E. Dueber, Dan Vasilescu, R. Dyche Mullins, Bruce J. Mayer, Wendell A. Lim

ABSTRACT

Enterohaemorrhagic Escherichia coli attaches to the intestine through actin pedestals that are formed when the bacterium injects its protein EspFU (also known as TccP) into host cells1. EspFU potently activates the host WASP (Wiskott–Aldrich syndrome protein) family of actin-nucleating factors, which are normally activated by the GTPase CDC42, among other signalling molecules. Apart from its amino-terminal type III secretion signal, EspFU consists of five-and-a-half 47-amino-acid repeats. Here we show that a 17-residue motif within this EspFU repeat is sufficient for interaction with N-WASP (also known as WASL). Unlike most pathogen proteins that interface with the cytoskeletal machinery, this motif does not mimic natural upstream activators: instead of mimicking an activated state of CDC42, EspFU mimics an autoinhibitory element found within N-WASP. Thus, EspFU activates N-WASP by competitively disrupting the autoinhibited state. By mimicking an internal regulatory element and not the natural activator, EspFU selectively activates only a precise subset of CDC42-activated processes. Although one repeat is able to stimulate actin polymerization, we show that multiple-repeat fragments have notably increased potency. The activities of these EspFU fragments correlate with their ability to coordinate activation of at least two N-WASP proteins. Thus, this pathogen has used a simple autoinhibitory fragment as a component to build a highly effective actin polymerization machine. More... »

PAGES

1005-1008

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nature07170

DOI

http://dx.doi.org/10.1038/nature07170

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1032710589

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18650806


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