Pyruvate kinase M2 is a phosphotyrosine-binding protein View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-03

AUTHORS

Heather R. Christofk, Matthew G. Vander Heiden, Ning Wu, John M. Asara, Lewis C. Cantley

ABSTRACT

Growth factors stimulate cells to take up excess nutrients and to use them for anabolic processes. The biochemical mechanism by which this is accomplished is not fully understood but it is initiated by phosphorylation of signalling proteins on tyrosine residues. Using a novel proteomic screen for phosphotyrosine-binding proteins, we have made the observation that an enzyme involved in glycolysis, the human M2 (fetal) isoform of pyruvate kinase (PKM2), binds directly and selectively to tyrosine-phosphorylated peptides. We show that binding of phosphotyrosine peptides to PKM2 results in release of the allosteric activator fructose-1,6-bisphosphate, leading to inhibition of PKM2 enzymatic activity. We also provide evidence that this regulation of PKM2 by phosphotyrosine signalling diverts glucose metabolites from energy production to anabolic processes when cells are stimulated by certain growth factors. Collectively, our results indicate that expression of this phosphotyrosine-binding form of pyruvate kinase is critical for rapid growth in cancer cells. More... »

PAGES

181

Journal

TITLE

Nature

ISSUE

7184

VOLUME

452

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nature06667

DOI

http://dx.doi.org/10.1038/nature06667

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1030770844

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18337815


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