Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-01

AUTHORS

Shota Atsumi, Taizo Hanai, James C. Liao

ABSTRACT

Global energy and environmental problems have stimulated increased efforts towards synthesizing biofuels from renewable resources. Compared to the traditional biofuel, ethanol, higher alcohols offer advantages as gasoline substitutes because of their higher energy density and lower hygroscopicity. In addition, branched-chain alcohols have higher octane numbers compared with their straight-chain counterparts. However, these alcohols cannot be synthesized economically using native organisms. Here we present a metabolic engineering approach using Escherichia coli to produce higher alcohols including isobutanol, 1-butanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol from glucose, a renewable carbon source. This strategy uses the host's highly active amino acid biosynthetic pathway and diverts its 2-keto acid intermediates for alcohol synthesis. In particular, we have achieved high-yield, high-specificity production of isobutanol from glucose. The strategy enables the exploration of biofuels beyond those naturally accumulated to high quantities in microbial fermentation. More... »

PAGES

86

References to SciGraph publications

Journal

TITLE

Nature

ISSUE

7174

VOLUME

451

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nature06450

    DOI

    http://dx.doi.org/10.1038/nature06450

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1048303418

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/18172501


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