The structural basis for activation of plant immunity by bacterial effector protein AvrPto View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-09

AUTHORS

Weiman Xing, Yan Zou, Qun Liu, Jianing Liu, Xi Luo, Qingqiu Huang, She Chen, Lihuang Zhu, Ruchang Bi, Quan Hao, Jia-Wei Wu, Jian-Min Zhou, Jijie Chai

ABSTRACT

Pathogenic microbes use effectors to enhance susceptibility in host plants. However, plants have evolved a sophisticated immune system to detect these effectors using cognate disease resistance proteins, a recognition that is highly specific, often elicits rapid and localized cell death, known as a hypersensitive response, and thus potentially limits pathogen growth. Despite numerous genetic and biochemical studies on the interactions between pathogen effector proteins and plant resistance proteins, the structural bases for such interactions remain elusive. The direct interaction between the tomato protein kinase Pto and the Pseudomonas syringae effector protein AvrPto is known to trigger disease resistance and programmed cell death through the nucleotide-binding site/leucine-rich repeat (NBS-LRR) class of disease resistance protein Prf. Here we present the crystal structure of an AvrPto-Pto complex. Contrary to the widely held hypothesis that AvrPto activates Pto kinase activity, our structural and biochemical analyses demonstrated that AvrPto is an inhibitor of Pto kinase in vitro. The AvrPto-Pto interaction is mediated by the phosphorylation-stabilized P+1 loop and a second loop in Pto, both of which negatively regulate the Prf-mediated defences in the absence of AvrPto in tomato plants. Together, our results show that AvrPto derepresses host defences by interacting with the two defence-inhibition loops of Pto. More... »

PAGES

243

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nature06109

DOI

http://dx.doi.org/10.1038/nature06109

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1032614022

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17694048


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bacterial Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Crystallography, X-Ray", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lycopersicon esculentum", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphorylation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plant Diseases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Kinase Inhibitors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Kinases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pseudomonas syringae", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "National Institute of Biological Sciences, Beijing", 
          "id": "https://www.grid.ac/institutes/grid.410717.4", 
          "name": [
            "National Institute of Biological Sciences, No. 7 Science Park Road, Beijing 102206, China", 
            "Institute of Biophysics,"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xing", 
        "givenName": "Weiman", 
        "id": "sg:person.01107131364.59", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01107131364.59"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Genetics and Developmental Biology", 
          "id": "https://www.grid.ac/institutes/grid.418558.5", 
          "name": [
            "National Institute of Biological Sciences, No. 7 Science Park Road, Beijing 102206, China", 
            "Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zou", 
        "givenName": "Yan", 
        "id": "sg:person.01214157243.71", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01214157243.71"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Cornell University", 
          "id": "https://www.grid.ac/institutes/grid.5386.8", 
          "name": [
            "Cornell High-Energy Synchrotron Source, Cornell University, Ithaca, New York 14853, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liu", 
        "givenName": "Qun", 
        "id": "sg:person.01151737116.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01151737116.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Biological Sciences, Beijing", 
          "id": "https://www.grid.ac/institutes/grid.410717.4", 
          "name": [
            "National Institute of Biological Sciences, No. 7 Science Park Road, Beijing 102206, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liu", 
        "givenName": "Jianing", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Biological Sciences, Beijing", 
          "id": "https://www.grid.ac/institutes/grid.410717.4", 
          "name": [
            "National Institute of Biological Sciences, No. 7 Science Park Road, Beijing 102206, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Luo", 
        "givenName": "Xi", 
        "id": "sg:person.0632135241.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0632135241.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Genetics and Developmental Biology", 
          "id": "https://www.grid.ac/institutes/grid.418558.5", 
          "name": [
            "Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Huang", 
        "givenName": "Qingqiu", 
        "id": "sg:person.01074400210.78", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01074400210.78"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Biological Sciences, Beijing", 
          "id": "https://www.grid.ac/institutes/grid.410717.4", 
          "name": [
            "National Institute of Biological Sciences, No. 7 Science Park Road, Beijing 102206, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chen", 
        "givenName": "She", 
        "id": "sg:person.012131507277.81", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012131507277.81"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Genetics and Developmental Biology", 
          "id": "https://www.grid.ac/institutes/grid.418558.5", 
          "name": [
            "Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhu", 
        "givenName": "Lihuang", 
        "id": "sg:person.01244372656.60", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244372656.60"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Institute of Biophysics,"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bi", 
        "givenName": "Ruchang", 
        "id": "sg:person.0611366642.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611366642.17"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Cornell University", 
          "id": "https://www.grid.ac/institutes/grid.5386.8", 
          "name": [
            "Cornell High-Energy Synchrotron Source, Cornell University, Ithaca, New York 14853, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hao", 
        "givenName": "Quan", 
        "id": "sg:person.0771027361.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0771027361.26"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Tsinghua University", 
          "id": "https://www.grid.ac/institutes/grid.12527.33", 
          "name": [
            "Department of Biological Sciences and Biotechnology, Tsinghua University, 100084, Beijing, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wu", 
        "givenName": "Jia-Wei", 
        "id": "sg:person.01123755765.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123755765.47"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Biological Sciences, Beijing", 
          "id": "https://www.grid.ac/institutes/grid.410717.4", 
          "name": [
            "National Institute of Biological Sciences, No. 7 Science Park Road, Beijing 102206, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhou", 
        "givenName": "Jian-Min", 
        "id": "sg:person.0700747076.84", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0700747076.84"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Biological Sciences, Beijing", 
          "id": "https://www.grid.ac/institutes/grid.410717.4", 
          "name": [
            "National Institute of Biological Sciences, No. 7 Science Park Road, Beijing 102206, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chai", 
        "givenName": "Jijie", 
        "id": "sg:person.01342040611.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342040611.97"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1093/emboj/18.12.3232", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000009088"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0076-6879(97)76066-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004907170"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.cellbio.17.1.53", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006681301"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.104.040410", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008453297"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)80083-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011646452"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0076-6879(03)74002-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012321359"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.104.024141", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013545241"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0108767390010224", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014210169"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444996012255", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020405374"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(02)00741-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020670930"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.98.4.2059", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023738481"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2006.02.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023852338"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.str.2004.04.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024099460"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2004.08.024", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025770611"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1105/tpc.106.044016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034493117"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.pbi.2004.05.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036399123"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35102073", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036684777", 
          "https://doi.org/10.1038/35102073"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35102073", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036684777", 
          "https://doi.org/10.1038/35102073"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(02)00743-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038674410"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbamcr.2004.03.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038803380"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.py.09.090171.001423", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039031882"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1097-2765(00)80134-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046370113"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2006.02.047", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046603836"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.274.5295.2060", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046666314"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.274.5295.2063", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046793251"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.phyto.41.121602.143032", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046922429"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/19.10.2257", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049568588"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1094/mpmi.2002.15.3.281", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060080527"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1993.tb05725.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082784711"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083274811", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2007-09", 
    "datePublishedReg": "2007-09-01", 
    "description": "Pathogenic microbes use effectors to enhance susceptibility in host plants. However, plants have evolved a sophisticated immune system to detect these effectors using cognate disease resistance proteins, a recognition that is highly specific, often elicits rapid and localized cell death, known as a hypersensitive response, and thus potentially limits pathogen growth. Despite numerous genetic and biochemical studies on the interactions between pathogen effector proteins and plant resistance proteins, the structural bases for such interactions remain elusive. The direct interaction between the tomato protein kinase Pto and the Pseudomonas syringae effector protein AvrPto is known to trigger disease resistance and programmed cell death through the nucleotide-binding site/leucine-rich repeat (NBS-LRR) class of disease resistance protein Prf. Here we present the crystal structure of an AvrPto-Pto complex. Contrary to the widely held hypothesis that AvrPto activates Pto kinase activity, our structural and biochemical analyses demonstrated that AvrPto is an inhibitor of Pto kinase in vitro. The AvrPto-Pto interaction is mediated by the phosphorylation-stabilized P+1 loop and a second loop in Pto, both of which negatively regulate the Prf-mediated defences in the absence of AvrPto in tomato plants. Together, our results show that AvrPto derepresses host defences by interacting with the two defence-inhibition loops of Pto.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nature06109", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7159", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "449"
      }
    ], 
    "name": "The structural basis for activation of plant immunity by bacterial effector protein AvrPto", 
    "pagination": "243", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "9b5efe7a900f02f9c1f851dd616309c1ff7933896c742e48325d15c17b354c5d"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "17694048"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nature06109"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1032614022"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nature06109", 
      "https://app.dimensions.ai/details/publication/pub.1032614022"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T20:34", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8684_00000425.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/nature06109"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature06109'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature06109'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature06109'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature06109'


 

This table displays all metadata directly associated to this object as RDF triples.

284 TRIPLES      21 PREDICATES      66 URIs      29 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nature06109 schema:about N1129f3cb67e04d258bf6ec35beb647c0
2 N343bfbf50e3b4b9491a50c269a6365e7
3 N78a8ca0a87eb41d3947c0b2d98cdeca1
4 N7ca83be213384fb280d14659997ada8c
5 N9b54a0744f8242689b2ba7323ce491e3
6 Na43ea597a2d04f32a8a0d7877f13d0a6
7 Ncf0ada8073544d45809f353d9b6b980c
8 Ndec69ed79d1e4e92ab350bbe57bda16a
9 anzsrc-for:06
10 anzsrc-for:0601
11 schema:author N2f2db948497a48e78841e9da3650e86b
12 schema:citation sg:pub.10.1038/35102073
13 https://app.dimensions.ai/details/publication/pub.1083274811
14 https://doi.org/10.1002/j.1460-2075.1993.tb05725.x
15 https://doi.org/10.1016/j.bbamcr.2004.03.011
16 https://doi.org/10.1016/j.cell.2006.02.008
17 https://doi.org/10.1016/j.cell.2006.02.047
18 https://doi.org/10.1016/j.molcel.2004.08.024
19 https://doi.org/10.1016/j.pbi.2004.05.009
20 https://doi.org/10.1016/j.str.2004.04.017
21 https://doi.org/10.1016/s0076-6879(03)74002-6
22 https://doi.org/10.1016/s0076-6879(97)76066-x
23 https://doi.org/10.1016/s0092-8674(00)80083-5
24 https://doi.org/10.1016/s0092-8674(02)00741-9
25 https://doi.org/10.1016/s0092-8674(02)00743-2
26 https://doi.org/10.1016/s1097-2765(00)80134-3
27 https://doi.org/10.1073/pnas.98.4.2059
28 https://doi.org/10.1093/emboj/18.12.3232
29 https://doi.org/10.1093/emboj/19.10.2257
30 https://doi.org/10.1094/mpmi.2002.15.3.281
31 https://doi.org/10.1104/pp.104.040410
32 https://doi.org/10.1105/tpc.104.024141
33 https://doi.org/10.1105/tpc.106.044016
34 https://doi.org/10.1107/s0108767390010224
35 https://doi.org/10.1107/s0907444996012255
36 https://doi.org/10.1126/science.274.5295.2060
37 https://doi.org/10.1126/science.274.5295.2063
38 https://doi.org/10.1146/annurev.cellbio.17.1.53
39 https://doi.org/10.1146/annurev.phyto.41.121602.143032
40 https://doi.org/10.1146/annurev.py.09.090171.001423
41 schema:datePublished 2007-09
42 schema:datePublishedReg 2007-09-01
43 schema:description Pathogenic microbes use effectors to enhance susceptibility in host plants. However, plants have evolved a sophisticated immune system to detect these effectors using cognate disease resistance proteins, a recognition that is highly specific, often elicits rapid and localized cell death, known as a hypersensitive response, and thus potentially limits pathogen growth. Despite numerous genetic and biochemical studies on the interactions between pathogen effector proteins and plant resistance proteins, the structural bases for such interactions remain elusive. The direct interaction between the tomato protein kinase Pto and the Pseudomonas syringae effector protein AvrPto is known to trigger disease resistance and programmed cell death through the nucleotide-binding site/leucine-rich repeat (NBS-LRR) class of disease resistance protein Prf. Here we present the crystal structure of an AvrPto-Pto complex. Contrary to the widely held hypothesis that AvrPto activates Pto kinase activity, our structural and biochemical analyses demonstrated that AvrPto is an inhibitor of Pto kinase in vitro. The AvrPto-Pto interaction is mediated by the phosphorylation-stabilized P+1 loop and a second loop in Pto, both of which negatively regulate the Prf-mediated defences in the absence of AvrPto in tomato plants. Together, our results show that AvrPto derepresses host defences by interacting with the two defence-inhibition loops of Pto.
44 schema:genre research_article
45 schema:inLanguage en
46 schema:isAccessibleForFree false
47 schema:isPartOf N5eb545b0df43464a82b3727437156258
48 Nd4813be47df74bbba06f8016af2d232d
49 sg:journal.1018957
50 schema:name The structural basis for activation of plant immunity by bacterial effector protein AvrPto
51 schema:pagination 243
52 schema:productId N2280e9778d7645af9cd16ae965939602
53 N4e68a001a49843a7b105a6260be295b9
54 N9b8fbaea84224b70991da8b177ae33a2
55 N9f0458a633de4f5e8e4482d57e0d4a59
56 Ndfdf198d0cfc4348b2ef44815eef3eb4
57 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032614022
58 https://doi.org/10.1038/nature06109
59 schema:sdDatePublished 2019-04-10T20:34
60 schema:sdLicense https://scigraph.springernature.com/explorer/license/
61 schema:sdPublisher Nfc786f411f604555818c8c4b6896204b
62 schema:url https://www.nature.com/articles/nature06109
63 sgo:license sg:explorer/license/
64 sgo:sdDataset articles
65 rdf:type schema:ScholarlyArticle
66 N092476bdc1ca48a6a0c0061c37a83d02 rdf:first sg:person.01123755765.47
67 rdf:rest N637a6bee070246a4aca0ec5c90cbfb09
68 N10bd17bd9de943c3b8244593692ada30 rdf:first sg:person.01151737116.54
69 rdf:rest Nef56aef2b4164182978921825340185b
70 N1129f3cb67e04d258bf6ec35beb647c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
71 schema:name Plant Diseases
72 rdf:type schema:DefinedTerm
73 N1a7a7f1858d04283a5fa91348eb4d09b rdf:first sg:person.01214157243.71
74 rdf:rest N10bd17bd9de943c3b8244593692ada30
75 N2280e9778d7645af9cd16ae965939602 schema:name doi
76 schema:value 10.1038/nature06109
77 rdf:type schema:PropertyValue
78 N2f2db948497a48e78841e9da3650e86b rdf:first sg:person.01107131364.59
79 rdf:rest N1a7a7f1858d04283a5fa91348eb4d09b
80 N343bfbf50e3b4b9491a50c269a6365e7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name Protein Kinase Inhibitors
82 rdf:type schema:DefinedTerm
83 N35705238edbd4e1ab37f5e3d2d38fb3e rdf:first sg:person.012131507277.81
84 rdf:rest N667d5dfc77034c579d0d02170bef8df4
85 N4e68a001a49843a7b105a6260be295b9 schema:name nlm_unique_id
86 schema:value 0410462
87 rdf:type schema:PropertyValue
88 N59eed0ef3fdd4af9b17509f9ef6f3b12 rdf:first sg:person.01074400210.78
89 rdf:rest N35705238edbd4e1ab37f5e3d2d38fb3e
90 N5d6c07f1bbb240d2b51a3e97f221782a rdf:first sg:person.0632135241.07
91 rdf:rest N59eed0ef3fdd4af9b17509f9ef6f3b12
92 N5eb545b0df43464a82b3727437156258 schema:issueNumber 7159
93 rdf:type schema:PublicationIssue
94 N637a6bee070246a4aca0ec5c90cbfb09 rdf:first sg:person.0700747076.84
95 rdf:rest Nefa6e3afcfab4dd0947d4a3cfbf71e65
96 N667d5dfc77034c579d0d02170bef8df4 rdf:first sg:person.01244372656.60
97 rdf:rest N9ab7f87b02544123b8df52901b5c9a09
98 N78a8ca0a87eb41d3947c0b2d98cdeca1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Bacterial Proteins
100 rdf:type schema:DefinedTerm
101 N7ca83be213384fb280d14659997ada8c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Phosphorylation
103 rdf:type schema:DefinedTerm
104 N9aa7f0f502ef4e97b9f79132de787086 rdf:first sg:person.0771027361.26
105 rdf:rest N092476bdc1ca48a6a0c0061c37a83d02
106 N9ab7f87b02544123b8df52901b5c9a09 rdf:first sg:person.0611366642.17
107 rdf:rest N9aa7f0f502ef4e97b9f79132de787086
108 N9b54a0744f8242689b2ba7323ce491e3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Crystallography, X-Ray
110 rdf:type schema:DefinedTerm
111 N9b8fbaea84224b70991da8b177ae33a2 schema:name readcube_id
112 schema:value 9b5efe7a900f02f9c1f851dd616309c1ff7933896c742e48325d15c17b354c5d
113 rdf:type schema:PropertyValue
114 N9f0458a633de4f5e8e4482d57e0d4a59 schema:name dimensions_id
115 schema:value pub.1032614022
116 rdf:type schema:PropertyValue
117 N9f41c2d817954a9280118bf487ce602c schema:affiliation https://www.grid.ac/institutes/grid.410717.4
118 schema:familyName Liu
119 schema:givenName Jianing
120 rdf:type schema:Person
121 Na43ea597a2d04f32a8a0d7877f13d0a6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Pseudomonas syringae
123 rdf:type schema:DefinedTerm
124 Ncf0ada8073544d45809f353d9b6b980c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Lycopersicon esculentum
126 rdf:type schema:DefinedTerm
127 Nd4813be47df74bbba06f8016af2d232d schema:volumeNumber 449
128 rdf:type schema:PublicationVolume
129 Ndec69ed79d1e4e92ab350bbe57bda16a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Protein Kinases
131 rdf:type schema:DefinedTerm
132 Ndfdf198d0cfc4348b2ef44815eef3eb4 schema:name pubmed_id
133 schema:value 17694048
134 rdf:type schema:PropertyValue
135 Nef56aef2b4164182978921825340185b rdf:first N9f41c2d817954a9280118bf487ce602c
136 rdf:rest N5d6c07f1bbb240d2b51a3e97f221782a
137 Nefa6e3afcfab4dd0947d4a3cfbf71e65 rdf:first sg:person.01342040611.97
138 rdf:rest rdf:nil
139 Nf70648b7b5e24f2b9c9949f948ddd0f8 schema:name Institute of Biophysics,
140 rdf:type schema:Organization
141 Nfc786f411f604555818c8c4b6896204b schema:name Springer Nature - SN SciGraph project
142 rdf:type schema:Organization
143 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
144 schema:name Biological Sciences
145 rdf:type schema:DefinedTerm
146 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
147 schema:name Biochemistry and Cell Biology
148 rdf:type schema:DefinedTerm
149 sg:journal.1018957 schema:issn 0090-0028
150 1476-4687
151 schema:name Nature
152 rdf:type schema:Periodical
153 sg:person.01074400210.78 schema:affiliation https://www.grid.ac/institutes/grid.418558.5
154 schema:familyName Huang
155 schema:givenName Qingqiu
156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01074400210.78
157 rdf:type schema:Person
158 sg:person.01107131364.59 schema:affiliation https://www.grid.ac/institutes/grid.410717.4
159 schema:familyName Xing
160 schema:givenName Weiman
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01107131364.59
162 rdf:type schema:Person
163 sg:person.01123755765.47 schema:affiliation https://www.grid.ac/institutes/grid.12527.33
164 schema:familyName Wu
165 schema:givenName Jia-Wei
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123755765.47
167 rdf:type schema:Person
168 sg:person.01151737116.54 schema:affiliation https://www.grid.ac/institutes/grid.5386.8
169 schema:familyName Liu
170 schema:givenName Qun
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01151737116.54
172 rdf:type schema:Person
173 sg:person.012131507277.81 schema:affiliation https://www.grid.ac/institutes/grid.410717.4
174 schema:familyName Chen
175 schema:givenName She
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012131507277.81
177 rdf:type schema:Person
178 sg:person.01214157243.71 schema:affiliation https://www.grid.ac/institutes/grid.418558.5
179 schema:familyName Zou
180 schema:givenName Yan
181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01214157243.71
182 rdf:type schema:Person
183 sg:person.01244372656.60 schema:affiliation https://www.grid.ac/institutes/grid.418558.5
184 schema:familyName Zhu
185 schema:givenName Lihuang
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244372656.60
187 rdf:type schema:Person
188 sg:person.01342040611.97 schema:affiliation https://www.grid.ac/institutes/grid.410717.4
189 schema:familyName Chai
190 schema:givenName Jijie
191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342040611.97
192 rdf:type schema:Person
193 sg:person.0611366642.17 schema:affiliation Nf70648b7b5e24f2b9c9949f948ddd0f8
194 schema:familyName Bi
195 schema:givenName Ruchang
196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611366642.17
197 rdf:type schema:Person
198 sg:person.0632135241.07 schema:affiliation https://www.grid.ac/institutes/grid.410717.4
199 schema:familyName Luo
200 schema:givenName Xi
201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0632135241.07
202 rdf:type schema:Person
203 sg:person.0700747076.84 schema:affiliation https://www.grid.ac/institutes/grid.410717.4
204 schema:familyName Zhou
205 schema:givenName Jian-Min
206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0700747076.84
207 rdf:type schema:Person
208 sg:person.0771027361.26 schema:affiliation https://www.grid.ac/institutes/grid.5386.8
209 schema:familyName Hao
210 schema:givenName Quan
211 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0771027361.26
212 rdf:type schema:Person
213 sg:pub.10.1038/35102073 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036684777
214 https://doi.org/10.1038/35102073
215 rdf:type schema:CreativeWork
216 https://app.dimensions.ai/details/publication/pub.1083274811 schema:CreativeWork
217 https://doi.org/10.1002/j.1460-2075.1993.tb05725.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082784711
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1016/j.bbamcr.2004.03.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038803380
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1016/j.cell.2006.02.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023852338
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1016/j.cell.2006.02.047 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046603836
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1016/j.molcel.2004.08.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025770611
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1016/j.pbi.2004.05.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036399123
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1016/j.str.2004.04.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024099460
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1016/s0076-6879(03)74002-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012321359
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1016/s0076-6879(97)76066-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1004907170
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1016/s0092-8674(00)80083-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011646452
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1016/s0092-8674(02)00741-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020670930
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1016/s0092-8674(02)00743-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038674410
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1016/s1097-2765(00)80134-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046370113
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1073/pnas.98.4.2059 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023738481
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1093/emboj/18.12.3232 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000009088
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1093/emboj/19.10.2257 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049568588
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1094/mpmi.2002.15.3.281 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060080527
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1104/pp.104.040410 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008453297
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1105/tpc.104.024141 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013545241
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1105/tpc.106.044016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034493117
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1107/s0108767390010224 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014210169
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1107/s0907444996012255 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020405374
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1126/science.274.5295.2060 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046666314
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1126/science.274.5295.2063 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046793251
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1146/annurev.cellbio.17.1.53 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006681301
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1146/annurev.phyto.41.121602.143032 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046922429
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1146/annurev.py.09.090171.001423 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039031882
270 rdf:type schema:CreativeWork
271 https://www.grid.ac/institutes/grid.12527.33 schema:alternateName Tsinghua University
272 schema:name Department of Biological Sciences and Biotechnology, Tsinghua University, 100084, Beijing, China
273 rdf:type schema:Organization
274 https://www.grid.ac/institutes/grid.410717.4 schema:alternateName National Institute of Biological Sciences, Beijing
275 schema:name Institute of Biophysics,
276 National Institute of Biological Sciences, No. 7 Science Park Road, Beijing 102206, China
277 rdf:type schema:Organization
278 https://www.grid.ac/institutes/grid.418558.5 schema:alternateName Institute of Genetics and Developmental Biology
279 schema:name Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
280 National Institute of Biological Sciences, No. 7 Science Park Road, Beijing 102206, China
281 rdf:type schema:Organization
282 https://www.grid.ac/institutes/grid.5386.8 schema:alternateName Cornell University
283 schema:name Cornell High-Energy Synchrotron Source, Cornell University, Ithaca, New York 14853, USA
284 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...