CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-01

AUTHORS

Seiji Tanaka, Toshiko Umemori, Kazuyuki Hirai, Sachiko Muramatsu, Yoichiro Kamimura, Hiroyuki Araki

ABSTRACT

In eukaryotic cells, cyclin-dependent kinases (CDKs) have an important involvement at various points in the cell cycle. At the onset of S phase, active CDK is essential for chromosomal DNA replication, although its precise role is unknown. In budding yeast (Saccharomyces cerevisiae), the replication protein Sld2 (ref. 2) is an essential CDK substrate, but its phospho-mimetic form (Sld2-11D) alone neither affects cell growth nor promotes DNA replication in the absence of CDK activity, suggesting that other essential CDK substrates promote DNA replication. Here we show that both an allele of CDC45 (JET1) and high-copy DPB11, in combination with Sld2-11D, separately confer CDK-independent DNA replication. Although Cdc45 is not an essential CDK substrate, CDK-dependent phosphorylation of Sld3, which associates with Cdc45 (ref. 5), is essential and generates a binding site for Dpb11. Both the JET1 mutation and high-copy DPB11 by-pass the requirement for Sld3 phosphorylation in DNA replication. Because phosphorylated Sld2 binds to the carboxy-terminal pair of BRCT domains in Dpb11 (ref. 4), we propose that Dpb11 connects phosphorylated Sld2 and Sld3 to facilitate interactions between replication proteins, such as Cdc45 and GINS. Our results demonstrate that CDKs regulate interactions between BRCT-domain-containing replication proteins and other phosphorylated proteins for the initiation of chromosomal DNA replication; similar regulation may take place in higher eukaryotes. More... »

PAGES

328

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nature05465

DOI

http://dx.doi.org/10.1038/nature05465

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1042319022

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17167415


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Cycle Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromosomes, Fungal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cyclin-Dependent Kinases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Replication", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Fungal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA-Binding Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nuclear Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphorylation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Binding", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "S Phase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomycetales", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Japan Science and Technology Agency", 
          "id": "https://www.grid.ac/institutes/grid.419082.6", 
          "name": [
            "Division of Microbial Genetics, National Institute of Genetics, Research Organization of Information and Systems, and,", 
            "Department of Genetics, SOKENDAI, Yata 1111, Mishima, Shizuoka 411-8540, Japan", 
            "CREST, Kawaguchi, Saitama 332-0012, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tanaka", 
        "givenName": "Seiji", 
        "id": "sg:person.0654321475.75", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0654321475.75"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Japan Science and Technology Agency", 
          "id": "https://www.grid.ac/institutes/grid.419082.6", 
          "name": [
            "Division of Microbial Genetics, National Institute of Genetics, Research Organization of Information and Systems, and,", 
            "CREST, Kawaguchi, Saitama 332-0012, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Umemori", 
        "givenName": "Toshiko", 
        "id": "sg:person.01071206076.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01071206076.27"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Japan Science and Technology Agency", 
          "id": "https://www.grid.ac/institutes/grid.419082.6", 
          "name": [
            "Division of Microbial Genetics, National Institute of Genetics, Research Organization of Information and Systems, and,", 
            "CREST, Kawaguchi, Saitama 332-0012, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hirai", 
        "givenName": "Kazuyuki", 
        "id": "sg:person.01355370016.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01355370016.67"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Division of Microbial Genetics, National Institute of Genetics, Research Organization of Information and Systems, and,"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Muramatsu", 
        "givenName": "Sachiko", 
        "id": "sg:person.01205434476.75", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01205434476.75"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Japan Science and Technology Agency", 
          "id": "https://www.grid.ac/institutes/grid.419082.6", 
          "name": [
            "Division of Microbial Genetics, National Institute of Genetics, Research Organization of Information and Systems, and,", 
            "Department of Genetics, SOKENDAI, Yata 1111, Mishima, Shizuoka 411-8540, Japan", 
            "CREST, Kawaguchi, Saitama 332-0012, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kamimura", 
        "givenName": "Yoichiro", 
        "id": "sg:person.01321663076.98", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321663076.98"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Japan Science and Technology Agency", 
          "id": "https://www.grid.ac/institutes/grid.419082.6", 
          "name": [
            "Division of Microbial Genetics, National Institute of Genetics, Research Organization of Information and Systems, and,", 
            "Department of Genetics, SOKENDAI, Yata 1111, Mishima, Shizuoka 411-8540, Japan", 
            "CREST, Kawaguchi, Saitama 332-0012, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Araki", 
        "givenName": "Hiroyuki", 
        "id": "sg:person.015157102075.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015157102075.61"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1038/sj.emboj.7601075", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003172621"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35030148", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007129994", 
          "https://doi.org/10.1038/35030148"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35030148", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007129994", 
          "https://doi.org/10.1038/35030148"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cub.2004.09.019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007533389"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0602400103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009060164"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.92.25.11791", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009601194"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/20.8.2097", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010219249"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.dnarep.2005.04.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011349997"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.dnarep.2005.04.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011349997"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcp.10070", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012453312"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm1663", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012862888", 
          "https://doi.org/10.1038/nrm1663"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm1663", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012862888", 
          "https://doi.org/10.1038/nrm1663"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tibs.2004.09.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015162467"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05432", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017905987", 
          "https://doi.org/10.1038/nature05432"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05432", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017905987", 
          "https://doi.org/10.1038/nature05432"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb1382", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020448729", 
          "https://doi.org/10.1038/ncb1382"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb1382", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020448729", 
          "https://doi.org/10.1038/ncb1382"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/sj.emboj.7601063", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027987961"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/sj.emboj.7601063", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027987961"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(87)90642-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030825149"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.20.8.2809-2817.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035125901"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35082600", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036007845", 
          "https://doi.org/10.1038/35082600"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35082600", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036007845", 
          "https://doi.org/10.1038/35082600"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bies.10370", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038377320"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/15649", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039277346", 
          "https://doi.org/10.1038/15649"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/15649", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039277346", 
          "https://doi.org/10.1038/15649"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.23.8.2733-2748.2003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039698497"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.biochem.71.110601.135425", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040856314"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.18.10.6102", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045383676"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.1065903", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046475412"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2006.01.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047999181"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature713", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049620350", 
          "https://doi.org/10.1038/nature713"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature713", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049620350", 
          "https://doi.org/10.1038/nature713"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1097-2765(03)00441-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050158520"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083357036", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2007-01", 
    "datePublishedReg": "2007-01-01", 
    "description": "In eukaryotic cells, cyclin-dependent kinases (CDKs) have an important involvement at various points in the cell cycle. At the onset of S phase, active CDK is essential for chromosomal DNA replication, although its precise role is unknown. In budding yeast (Saccharomyces cerevisiae), the replication protein Sld2 (ref. 2) is an essential CDK substrate, but its phospho-mimetic form (Sld2-11D) alone neither affects cell growth nor promotes DNA replication in the absence of CDK activity, suggesting that other essential CDK substrates promote DNA replication. Here we show that both an allele of CDC45 (JET1) and high-copy DPB11, in combination with Sld2-11D, separately confer CDK-independent DNA replication. Although Cdc45 is not an essential CDK substrate, CDK-dependent phosphorylation of Sld3, which associates with Cdc45 (ref. 5), is essential and generates a binding site for Dpb11. Both the JET1 mutation and high-copy DPB11 by-pass the requirement for Sld3 phosphorylation in DNA replication. Because phosphorylated Sld2 binds to the carboxy-terminal pair of BRCT domains in Dpb11 (ref. 4), we propose that Dpb11 connects phosphorylated Sld2 and Sld3 to facilitate interactions between replication proteins, such as Cdc45 and GINS. Our results demonstrate that CDKs regulate interactions between BRCT-domain-containing replication proteins and other phosphorylated proteins for the initiation of chromosomal DNA replication; similar regulation may take place in higher eukaryotes.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nature05465", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7125", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "445"
      }
    ], 
    "name": "CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast", 
    "pagination": "328", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "c995a5cbcfb9e50a3b990b950dc46fd11e623a96c2d12751d4a7f0e82fbbb84c"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "17167415"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nature05465"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1042319022"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nature05465", 
      "https://app.dimensions.ai/details/publication/pub.1042319022"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:12", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000361_0000000361/records_53990_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/nature05465"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature05465'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature05465'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature05465'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature05465'


 

This table displays all metadata directly associated to this object as RDF triples.

240 TRIPLES      21 PREDICATES      67 URIs      33 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nature05465 schema:about N0d638e8a83c74beda54d293563f90a5a
2 N325d4fed800f4e42a43791e913e00491
3 N369635eef59947998f6caa613629e65f
4 N3889b696a60a4c3d9e2fc65042783cbe
5 N540a24d7a81c490593bdfcd3f405195a
6 N6d3d78c4f8834f8996d4debdf5441789
7 N71b3a8e2be374ba496d9ae4e414ebe07
8 N809a6a1de99a4c31923e96dc6f859195
9 Na7ec8996c9a14dcebf8044bf766212a1
10 Nb8e30cc87993481b9a0b7bc3cab0590b
11 Nf59a823fc2dd41b49a99237e68d503b6
12 Nffea6abb651c4ffdac991322f7f7ec5a
13 anzsrc-for:06
14 anzsrc-for:0601
15 schema:author N2a1a4c81a2c04353866a568676aeb27d
16 schema:citation sg:pub.10.1038/15649
17 sg:pub.10.1038/35030148
18 sg:pub.10.1038/35082600
19 sg:pub.10.1038/nature05432
20 sg:pub.10.1038/nature713
21 sg:pub.10.1038/ncb1382
22 sg:pub.10.1038/nrm1663
23 https://app.dimensions.ai/details/publication/pub.1083357036
24 https://doi.org/10.1002/bies.10370
25 https://doi.org/10.1002/jcp.10070
26 https://doi.org/10.1016/0092-8674(87)90642-8
27 https://doi.org/10.1016/j.cub.2004.09.019
28 https://doi.org/10.1016/j.dnarep.2005.04.001
29 https://doi.org/10.1016/j.molcel.2006.01.030
30 https://doi.org/10.1016/j.tibs.2004.09.010
31 https://doi.org/10.1016/s1097-2765(03)00441-6
32 https://doi.org/10.1038/sj.emboj.7601063
33 https://doi.org/10.1038/sj.emboj.7601075
34 https://doi.org/10.1073/pnas.0602400103
35 https://doi.org/10.1073/pnas.92.25.11791
36 https://doi.org/10.1093/emboj/20.8.2097
37 https://doi.org/10.1101/gad.1065903
38 https://doi.org/10.1128/mcb.18.10.6102
39 https://doi.org/10.1128/mcb.20.8.2809-2817.2000
40 https://doi.org/10.1128/mcb.23.8.2733-2748.2003
41 https://doi.org/10.1146/annurev.biochem.71.110601.135425
42 schema:datePublished 2007-01
43 schema:datePublishedReg 2007-01-01
44 schema:description In eukaryotic cells, cyclin-dependent kinases (CDKs) have an important involvement at various points in the cell cycle. At the onset of S phase, active CDK is essential for chromosomal DNA replication, although its precise role is unknown. In budding yeast (Saccharomyces cerevisiae), the replication protein Sld2 (ref. 2) is an essential CDK substrate, but its phospho-mimetic form (Sld2-11D) alone neither affects cell growth nor promotes DNA replication in the absence of CDK activity, suggesting that other essential CDK substrates promote DNA replication. Here we show that both an allele of CDC45 (JET1) and high-copy DPB11, in combination with Sld2-11D, separately confer CDK-independent DNA replication. Although Cdc45 is not an essential CDK substrate, CDK-dependent phosphorylation of Sld3, which associates with Cdc45 (ref. 5), is essential and generates a binding site for Dpb11. Both the JET1 mutation and high-copy DPB11 by-pass the requirement for Sld3 phosphorylation in DNA replication. Because phosphorylated Sld2 binds to the carboxy-terminal pair of BRCT domains in Dpb11 (ref. 4), we propose that Dpb11 connects phosphorylated Sld2 and Sld3 to facilitate interactions between replication proteins, such as Cdc45 and GINS. Our results demonstrate that CDKs regulate interactions between BRCT-domain-containing replication proteins and other phosphorylated proteins for the initiation of chromosomal DNA replication; similar regulation may take place in higher eukaryotes.
45 schema:genre research_article
46 schema:inLanguage en
47 schema:isAccessibleForFree false
48 schema:isPartOf N24f5b22c341745169739a6ec3d9dabea
49 N97e6b2a7aa7c495d904cb56040f62494
50 sg:journal.1018957
51 schema:name CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast
52 schema:pagination 328
53 schema:productId N3dd99017be8a4d57bb911b68dd459bcc
54 N5857d5a758c641b9aec5ae877bd61f48
55 N937b8b4e61804ca2a422216affaedc7f
56 N9bfecfc89bf74c2991fce345020bfbb0
57 Ndae9d186361a4583b2eaf7fd95b6aed2
58 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042319022
59 https://doi.org/10.1038/nature05465
60 schema:sdDatePublished 2019-04-11T12:12
61 schema:sdLicense https://scigraph.springernature.com/explorer/license/
62 schema:sdPublisher Nc3918655b2664602b8e72a6d8d58fd23
63 schema:url https://www.nature.com/articles/nature05465
64 sgo:license sg:explorer/license/
65 sgo:sdDataset articles
66 rdf:type schema:ScholarlyArticle
67 N0d638e8a83c74beda54d293563f90a5a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
68 schema:name Protein Binding
69 rdf:type schema:DefinedTerm
70 N24f5b22c341745169739a6ec3d9dabea schema:issueNumber 7125
71 rdf:type schema:PublicationIssue
72 N2a1a4c81a2c04353866a568676aeb27d rdf:first sg:person.0654321475.75
73 rdf:rest N8cd01b3d4cfd4db3acb36764b2096176
74 N325d4fed800f4e42a43791e913e00491 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
75 schema:name Cyclin-Dependent Kinases
76 rdf:type schema:DefinedTerm
77 N369635eef59947998f6caa613629e65f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
78 schema:name S Phase
79 rdf:type schema:DefinedTerm
80 N384c822dca4244329b5b5abb545ae57b rdf:first sg:person.01355370016.67
81 rdf:rest N77d165889b4a4ca6b9c9d01fccfeb668
82 N3889b696a60a4c3d9e2fc65042783cbe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Saccharomyces cerevisiae Proteins
84 rdf:type schema:DefinedTerm
85 N3dd99017be8a4d57bb911b68dd459bcc schema:name nlm_unique_id
86 schema:value 0410462
87 rdf:type schema:PropertyValue
88 N540a24d7a81c490593bdfcd3f405195a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Nuclear Proteins
90 rdf:type schema:DefinedTerm
91 N5857d5a758c641b9aec5ae877bd61f48 schema:name dimensions_id
92 schema:value pub.1042319022
93 rdf:type schema:PropertyValue
94 N6d3d78c4f8834f8996d4debdf5441789 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name DNA, Fungal
96 rdf:type schema:DefinedTerm
97 N71b3a8e2be374ba496d9ae4e414ebe07 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name DNA-Binding Proteins
99 rdf:type schema:DefinedTerm
100 N77d165889b4a4ca6b9c9d01fccfeb668 rdf:first sg:person.01205434476.75
101 rdf:rest Na2fb579f10874e069e372221326ba04b
102 N809a6a1de99a4c31923e96dc6f859195 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Chromosomes, Fungal
104 rdf:type schema:DefinedTerm
105 N8cd01b3d4cfd4db3acb36764b2096176 rdf:first sg:person.01071206076.27
106 rdf:rest N384c822dca4244329b5b5abb545ae57b
107 N9100c4d2a13e458c9d2ee2675ab53004 schema:name Division of Microbial Genetics, National Institute of Genetics, Research Organization of Information and Systems, and,
108 rdf:type schema:Organization
109 N937b8b4e61804ca2a422216affaedc7f schema:name readcube_id
110 schema:value c995a5cbcfb9e50a3b990b950dc46fd11e623a96c2d12751d4a7f0e82fbbb84c
111 rdf:type schema:PropertyValue
112 N97e6b2a7aa7c495d904cb56040f62494 schema:volumeNumber 445
113 rdf:type schema:PublicationVolume
114 N9bfecfc89bf74c2991fce345020bfbb0 schema:name doi
115 schema:value 10.1038/nature05465
116 rdf:type schema:PropertyValue
117 Na2fb579f10874e069e372221326ba04b rdf:first sg:person.01321663076.98
118 rdf:rest Nd5430bcfebe74e92a3b5a47008cb7543
119 Na7ec8996c9a14dcebf8044bf766212a1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name DNA Replication
121 rdf:type schema:DefinedTerm
122 Nb8e30cc87993481b9a0b7bc3cab0590b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Cell Cycle Proteins
124 rdf:type schema:DefinedTerm
125 Nc3918655b2664602b8e72a6d8d58fd23 schema:name Springer Nature - SN SciGraph project
126 rdf:type schema:Organization
127 Nd5430bcfebe74e92a3b5a47008cb7543 rdf:first sg:person.015157102075.61
128 rdf:rest rdf:nil
129 Ndae9d186361a4583b2eaf7fd95b6aed2 schema:name pubmed_id
130 schema:value 17167415
131 rdf:type schema:PropertyValue
132 Nf59a823fc2dd41b49a99237e68d503b6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Saccharomycetales
134 rdf:type schema:DefinedTerm
135 Nffea6abb651c4ffdac991322f7f7ec5a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Phosphorylation
137 rdf:type schema:DefinedTerm
138 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
139 schema:name Biological Sciences
140 rdf:type schema:DefinedTerm
141 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
142 schema:name Biochemistry and Cell Biology
143 rdf:type schema:DefinedTerm
144 sg:journal.1018957 schema:issn 0090-0028
145 1476-4687
146 schema:name Nature
147 rdf:type schema:Periodical
148 sg:person.01071206076.27 schema:affiliation https://www.grid.ac/institutes/grid.419082.6
149 schema:familyName Umemori
150 schema:givenName Toshiko
151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01071206076.27
152 rdf:type schema:Person
153 sg:person.01205434476.75 schema:affiliation N9100c4d2a13e458c9d2ee2675ab53004
154 schema:familyName Muramatsu
155 schema:givenName Sachiko
156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01205434476.75
157 rdf:type schema:Person
158 sg:person.01321663076.98 schema:affiliation https://www.grid.ac/institutes/grid.419082.6
159 schema:familyName Kamimura
160 schema:givenName Yoichiro
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321663076.98
162 rdf:type schema:Person
163 sg:person.01355370016.67 schema:affiliation https://www.grid.ac/institutes/grid.419082.6
164 schema:familyName Hirai
165 schema:givenName Kazuyuki
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01355370016.67
167 rdf:type schema:Person
168 sg:person.015157102075.61 schema:affiliation https://www.grid.ac/institutes/grid.419082.6
169 schema:familyName Araki
170 schema:givenName Hiroyuki
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015157102075.61
172 rdf:type schema:Person
173 sg:person.0654321475.75 schema:affiliation https://www.grid.ac/institutes/grid.419082.6
174 schema:familyName Tanaka
175 schema:givenName Seiji
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0654321475.75
177 rdf:type schema:Person
178 sg:pub.10.1038/15649 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039277346
179 https://doi.org/10.1038/15649
180 rdf:type schema:CreativeWork
181 sg:pub.10.1038/35030148 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007129994
182 https://doi.org/10.1038/35030148
183 rdf:type schema:CreativeWork
184 sg:pub.10.1038/35082600 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036007845
185 https://doi.org/10.1038/35082600
186 rdf:type schema:CreativeWork
187 sg:pub.10.1038/nature05432 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017905987
188 https://doi.org/10.1038/nature05432
189 rdf:type schema:CreativeWork
190 sg:pub.10.1038/nature713 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049620350
191 https://doi.org/10.1038/nature713
192 rdf:type schema:CreativeWork
193 sg:pub.10.1038/ncb1382 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020448729
194 https://doi.org/10.1038/ncb1382
195 rdf:type schema:CreativeWork
196 sg:pub.10.1038/nrm1663 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012862888
197 https://doi.org/10.1038/nrm1663
198 rdf:type schema:CreativeWork
199 https://app.dimensions.ai/details/publication/pub.1083357036 schema:CreativeWork
200 https://doi.org/10.1002/bies.10370 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038377320
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1002/jcp.10070 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012453312
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1016/0092-8674(87)90642-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030825149
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1016/j.cub.2004.09.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007533389
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1016/j.dnarep.2005.04.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011349997
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1016/j.molcel.2006.01.030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047999181
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1016/j.tibs.2004.09.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015162467
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1016/s1097-2765(03)00441-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050158520
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1038/sj.emboj.7601063 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027987961
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1038/sj.emboj.7601075 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003172621
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1073/pnas.0602400103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009060164
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1073/pnas.92.25.11791 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009601194
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1093/emboj/20.8.2097 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010219249
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1101/gad.1065903 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046475412
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1128/mcb.18.10.6102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045383676
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1128/mcb.20.8.2809-2817.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035125901
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1128/mcb.23.8.2733-2748.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039698497
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1146/annurev.biochem.71.110601.135425 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040856314
235 rdf:type schema:CreativeWork
236 https://www.grid.ac/institutes/grid.419082.6 schema:alternateName Japan Science and Technology Agency
237 schema:name CREST, Kawaguchi, Saitama 332-0012, Japan
238 Department of Genetics, SOKENDAI, Yata 1111, Mishima, Shizuoka 411-8540, Japan
239 Division of Microbial Genetics, National Institute of Genetics, Research Organization of Information and Systems, and,
240 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...