CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-01

AUTHORS

Seiji Tanaka, Toshiko Umemori, Kazuyuki Hirai, Sachiko Muramatsu, Yoichiro Kamimura, Hiroyuki Araki

ABSTRACT

In eukaryotic cells, cyclin-dependent kinases (CDKs) have an important involvement at various points in the cell cycle. At the onset of S phase, active CDK is essential for chromosomal DNA replication, although its precise role is unknown. In budding yeast (Saccharomyces cerevisiae), the replication protein Sld2 (ref. 2) is an essential CDK substrate, but its phospho-mimetic form (Sld2-11D) alone neither affects cell growth nor promotes DNA replication in the absence of CDK activity, suggesting that other essential CDK substrates promote DNA replication. Here we show that both an allele of CDC45 (JET1) and high-copy DPB11, in combination with Sld2-11D, separately confer CDK-independent DNA replication. Although Cdc45 is not an essential CDK substrate, CDK-dependent phosphorylation of Sld3, which associates with Cdc45 (ref. 5), is essential and generates a binding site for Dpb11. Both the JET1 mutation and high-copy DPB11 by-pass the requirement for Sld3 phosphorylation in DNA replication. Because phosphorylated Sld2 binds to the carboxy-terminal pair of BRCT domains in Dpb11 (ref. 4), we propose that Dpb11 connects phosphorylated Sld2 and Sld3 to facilitate interactions between replication proteins, such as Cdc45 and GINS. Our results demonstrate that CDKs regulate interactions between BRCT-domain-containing replication proteins and other phosphorylated proteins for the initiation of chromosomal DNA replication; similar regulation may take place in higher eukaryotes. More... »

PAGES

328

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nature05465

DOI

http://dx.doi.org/10.1038/nature05465

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1042319022

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17167415


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Cycle Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromosomes, Fungal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cyclin-Dependent Kinases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Replication", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Fungal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA-Binding Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nuclear Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphorylation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Binding", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "S Phase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomycetales", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Japan Science and Technology Agency", 
          "id": "https://www.grid.ac/institutes/grid.419082.6", 
          "name": [
            "Division of Microbial Genetics, National Institute of Genetics, Research Organization of Information and Systems, and,", 
            "Department of Genetics, SOKENDAI, Yata 1111, Mishima, Shizuoka 411-8540, Japan", 
            "CREST, Kawaguchi, Saitama 332-0012, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tanaka", 
        "givenName": "Seiji", 
        "id": "sg:person.0654321475.75", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0654321475.75"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Japan Science and Technology Agency", 
          "id": "https://www.grid.ac/institutes/grid.419082.6", 
          "name": [
            "Division of Microbial Genetics, National Institute of Genetics, Research Organization of Information and Systems, and,", 
            "CREST, Kawaguchi, Saitama 332-0012, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Umemori", 
        "givenName": "Toshiko", 
        "id": "sg:person.01071206076.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01071206076.27"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Japan Science and Technology Agency", 
          "id": "https://www.grid.ac/institutes/grid.419082.6", 
          "name": [
            "Division of Microbial Genetics, National Institute of Genetics, Research Organization of Information and Systems, and,", 
            "CREST, Kawaguchi, Saitama 332-0012, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hirai", 
        "givenName": "Kazuyuki", 
        "id": "sg:person.01355370016.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01355370016.67"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Division of Microbial Genetics, National Institute of Genetics, Research Organization of Information and Systems, and,"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Muramatsu", 
        "givenName": "Sachiko", 
        "id": "sg:person.01205434476.75", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01205434476.75"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Japan Science and Technology Agency", 
          "id": "https://www.grid.ac/institutes/grid.419082.6", 
          "name": [
            "Division of Microbial Genetics, National Institute of Genetics, Research Organization of Information and Systems, and,", 
            "Department of Genetics, SOKENDAI, Yata 1111, Mishima, Shizuoka 411-8540, Japan", 
            "CREST, Kawaguchi, Saitama 332-0012, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kamimura", 
        "givenName": "Yoichiro", 
        "id": "sg:person.01321663076.98", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321663076.98"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Japan Science and Technology Agency", 
          "id": "https://www.grid.ac/institutes/grid.419082.6", 
          "name": [
            "Division of Microbial Genetics, National Institute of Genetics, Research Organization of Information and Systems, and,", 
            "Department of Genetics, SOKENDAI, Yata 1111, Mishima, Shizuoka 411-8540, Japan", 
            "CREST, Kawaguchi, Saitama 332-0012, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Araki", 
        "givenName": "Hiroyuki", 
        "id": "sg:person.015157102075.61", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015157102075.61"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1038/sj.emboj.7601075", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003172621"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35030148", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007129994", 
          "https://doi.org/10.1038/35030148"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35030148", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007129994", 
          "https://doi.org/10.1038/35030148"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cub.2004.09.019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007533389"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0602400103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009060164"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.92.25.11791", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009601194"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/20.8.2097", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010219249"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.dnarep.2005.04.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011349997"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.dnarep.2005.04.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011349997"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcp.10070", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012453312"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm1663", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012862888", 
          "https://doi.org/10.1038/nrm1663"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm1663", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012862888", 
          "https://doi.org/10.1038/nrm1663"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tibs.2004.09.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015162467"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05432", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017905987", 
          "https://doi.org/10.1038/nature05432"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05432", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017905987", 
          "https://doi.org/10.1038/nature05432"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb1382", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020448729", 
          "https://doi.org/10.1038/ncb1382"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb1382", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020448729", 
          "https://doi.org/10.1038/ncb1382"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/sj.emboj.7601063", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027987961"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/sj.emboj.7601063", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027987961"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(87)90642-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030825149"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.20.8.2809-2817.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035125901"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35082600", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036007845", 
          "https://doi.org/10.1038/35082600"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35082600", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036007845", 
          "https://doi.org/10.1038/35082600"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bies.10370", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038377320"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/15649", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039277346", 
          "https://doi.org/10.1038/15649"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/15649", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039277346", 
          "https://doi.org/10.1038/15649"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.23.8.2733-2748.2003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039698497"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.biochem.71.110601.135425", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040856314"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.18.10.6102", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045383676"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.1065903", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046475412"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2006.01.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047999181"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature713", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049620350", 
          "https://doi.org/10.1038/nature713"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature713", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049620350", 
          "https://doi.org/10.1038/nature713"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1097-2765(03)00441-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050158520"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083357036", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2007-01", 
    "datePublishedReg": "2007-01-01", 
    "description": "In eukaryotic cells, cyclin-dependent kinases (CDKs) have an important involvement at various points in the cell cycle. At the onset of S phase, active CDK is essential for chromosomal DNA replication, although its precise role is unknown. In budding yeast (Saccharomyces cerevisiae), the replication protein Sld2 (ref. 2) is an essential CDK substrate, but its phospho-mimetic form (Sld2-11D) alone neither affects cell growth nor promotes DNA replication in the absence of CDK activity, suggesting that other essential CDK substrates promote DNA replication. Here we show that both an allele of CDC45 (JET1) and high-copy DPB11, in combination with Sld2-11D, separately confer CDK-independent DNA replication. Although Cdc45 is not an essential CDK substrate, CDK-dependent phosphorylation of Sld3, which associates with Cdc45 (ref. 5), is essential and generates a binding site for Dpb11. Both the JET1 mutation and high-copy DPB11 by-pass the requirement for Sld3 phosphorylation in DNA replication. Because phosphorylated Sld2 binds to the carboxy-terminal pair of BRCT domains in Dpb11 (ref. 4), we propose that Dpb11 connects phosphorylated Sld2 and Sld3 to facilitate interactions between replication proteins, such as Cdc45 and GINS. Our results demonstrate that CDKs regulate interactions between BRCT-domain-containing replication proteins and other phosphorylated proteins for the initiation of chromosomal DNA replication; similar regulation may take place in higher eukaryotes.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nature05465", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7125", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "445"
      }
    ], 
    "name": "CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast", 
    "pagination": "328", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "c995a5cbcfb9e50a3b990b950dc46fd11e623a96c2d12751d4a7f0e82fbbb84c"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "17167415"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nature05465"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1042319022"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nature05465", 
      "https://app.dimensions.ai/details/publication/pub.1042319022"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:12", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000361_0000000361/records_53990_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/nature05465"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature05465'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature05465'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature05465'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature05465'


 

This table displays all metadata directly associated to this object as RDF triples.

240 TRIPLES      21 PREDICATES      67 URIs      33 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nature05465 schema:about N017c2f370b494f118bbb4320ac2d4196
2 N0d6242aeb4d04e9c93719fdba62c0773
3 N1ca6ec176c3a4a1fbf6ed72e88a9e03e
4 N38efa8d5452742f288233cf6a0227ad8
5 N399e47981eb047e08a05aa6863f12bbe
6 N7033d2b1484547f5a6bedc7abda08e77
7 N762810f1ea224dc59275bc87bd4042b9
8 N7b08f1b8137346b488ec8f0915d60e0c
9 N85eb7675c2c544a28a5cf7bf36bfb64e
10 Na6cc1d31044c422999cd0399719cc3a0
11 Ndcd82a58ff39470abe0ccda0f17c0ff1
12 Nfabdd197f2d946b4984689ec9821bc9e
13 anzsrc-for:06
14 anzsrc-for:0601
15 schema:author N8acf84bf7b5c4135bf9af130c459d260
16 schema:citation sg:pub.10.1038/15649
17 sg:pub.10.1038/35030148
18 sg:pub.10.1038/35082600
19 sg:pub.10.1038/nature05432
20 sg:pub.10.1038/nature713
21 sg:pub.10.1038/ncb1382
22 sg:pub.10.1038/nrm1663
23 https://app.dimensions.ai/details/publication/pub.1083357036
24 https://doi.org/10.1002/bies.10370
25 https://doi.org/10.1002/jcp.10070
26 https://doi.org/10.1016/0092-8674(87)90642-8
27 https://doi.org/10.1016/j.cub.2004.09.019
28 https://doi.org/10.1016/j.dnarep.2005.04.001
29 https://doi.org/10.1016/j.molcel.2006.01.030
30 https://doi.org/10.1016/j.tibs.2004.09.010
31 https://doi.org/10.1016/s1097-2765(03)00441-6
32 https://doi.org/10.1038/sj.emboj.7601063
33 https://doi.org/10.1038/sj.emboj.7601075
34 https://doi.org/10.1073/pnas.0602400103
35 https://doi.org/10.1073/pnas.92.25.11791
36 https://doi.org/10.1093/emboj/20.8.2097
37 https://doi.org/10.1101/gad.1065903
38 https://doi.org/10.1128/mcb.18.10.6102
39 https://doi.org/10.1128/mcb.20.8.2809-2817.2000
40 https://doi.org/10.1128/mcb.23.8.2733-2748.2003
41 https://doi.org/10.1146/annurev.biochem.71.110601.135425
42 schema:datePublished 2007-01
43 schema:datePublishedReg 2007-01-01
44 schema:description In eukaryotic cells, cyclin-dependent kinases (CDKs) have an important involvement at various points in the cell cycle. At the onset of S phase, active CDK is essential for chromosomal DNA replication, although its precise role is unknown. In budding yeast (Saccharomyces cerevisiae), the replication protein Sld2 (ref. 2) is an essential CDK substrate, but its phospho-mimetic form (Sld2-11D) alone neither affects cell growth nor promotes DNA replication in the absence of CDK activity, suggesting that other essential CDK substrates promote DNA replication. Here we show that both an allele of CDC45 (JET1) and high-copy DPB11, in combination with Sld2-11D, separately confer CDK-independent DNA replication. Although Cdc45 is not an essential CDK substrate, CDK-dependent phosphorylation of Sld3, which associates with Cdc45 (ref. 5), is essential and generates a binding site for Dpb11. Both the JET1 mutation and high-copy DPB11 by-pass the requirement for Sld3 phosphorylation in DNA replication. Because phosphorylated Sld2 binds to the carboxy-terminal pair of BRCT domains in Dpb11 (ref. 4), we propose that Dpb11 connects phosphorylated Sld2 and Sld3 to facilitate interactions between replication proteins, such as Cdc45 and GINS. Our results demonstrate that CDKs regulate interactions between BRCT-domain-containing replication proteins and other phosphorylated proteins for the initiation of chromosomal DNA replication; similar regulation may take place in higher eukaryotes.
45 schema:genre research_article
46 schema:inLanguage en
47 schema:isAccessibleForFree false
48 schema:isPartOf N835e99ab025e45daaa9f2496f1d4ec85
49 N83d190882ec047098ecc50603f06a694
50 sg:journal.1018957
51 schema:name CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast
52 schema:pagination 328
53 schema:productId N0bcfccc7724b4d488048487869423e5e
54 N454ca8d69da947f1b836820b67e3c7b2
55 N543718ed84334656b384aae337b4613d
56 N875d9a6f90ee4c4f95f1fa8a1fbdf4a8
57 Ne8d87af3011a4cdda449a396231c2d24
58 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042319022
59 https://doi.org/10.1038/nature05465
60 schema:sdDatePublished 2019-04-11T12:12
61 schema:sdLicense https://scigraph.springernature.com/explorer/license/
62 schema:sdPublisher Nd2ae663536514717a9ff6e6290c86c77
63 schema:url https://www.nature.com/articles/nature05465
64 sgo:license sg:explorer/license/
65 sgo:sdDataset articles
66 rdf:type schema:ScholarlyArticle
67 N017c2f370b494f118bbb4320ac2d4196 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
68 schema:name Saccharomyces cerevisiae Proteins
69 rdf:type schema:DefinedTerm
70 N0bcfccc7724b4d488048487869423e5e schema:name pubmed_id
71 schema:value 17167415
72 rdf:type schema:PropertyValue
73 N0d6242aeb4d04e9c93719fdba62c0773 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
74 schema:name Protein Binding
75 rdf:type schema:DefinedTerm
76 N1ca6ec176c3a4a1fbf6ed72e88a9e03e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
77 schema:name S Phase
78 rdf:type schema:DefinedTerm
79 N3001031c7e3e48d1b9fee9d96634f153 rdf:first sg:person.01321663076.98
80 rdf:rest N6bca92ac48464f32814b680526babdca
81 N38efa8d5452742f288233cf6a0227ad8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Cyclin-Dependent Kinases
83 rdf:type schema:DefinedTerm
84 N399e47981eb047e08a05aa6863f12bbe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name DNA-Binding Proteins
86 rdf:type schema:DefinedTerm
87 N454ca8d69da947f1b836820b67e3c7b2 schema:name dimensions_id
88 schema:value pub.1042319022
89 rdf:type schema:PropertyValue
90 N543718ed84334656b384aae337b4613d schema:name doi
91 schema:value 10.1038/nature05465
92 rdf:type schema:PropertyValue
93 N6733d30ae1f0413886caf924a1466851 schema:name Division of Microbial Genetics, National Institute of Genetics, Research Organization of Information and Systems, and,
94 rdf:type schema:Organization
95 N6bca92ac48464f32814b680526babdca rdf:first sg:person.015157102075.61
96 rdf:rest rdf:nil
97 N7033d2b1484547f5a6bedc7abda08e77 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Saccharomycetales
99 rdf:type schema:DefinedTerm
100 N762810f1ea224dc59275bc87bd4042b9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Nuclear Proteins
102 rdf:type schema:DefinedTerm
103 N7b08f1b8137346b488ec8f0915d60e0c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Chromosomes, Fungal
105 rdf:type schema:DefinedTerm
106 N835e99ab025e45daaa9f2496f1d4ec85 schema:volumeNumber 445
107 rdf:type schema:PublicationVolume
108 N83d190882ec047098ecc50603f06a694 schema:issueNumber 7125
109 rdf:type schema:PublicationIssue
110 N85eb7675c2c544a28a5cf7bf36bfb64e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Phosphorylation
112 rdf:type schema:DefinedTerm
113 N875d9a6f90ee4c4f95f1fa8a1fbdf4a8 schema:name readcube_id
114 schema:value c995a5cbcfb9e50a3b990b950dc46fd11e623a96c2d12751d4a7f0e82fbbb84c
115 rdf:type schema:PropertyValue
116 N8acf84bf7b5c4135bf9af130c459d260 rdf:first sg:person.0654321475.75
117 rdf:rest N99812801a15945b6bf1c2307b9a34ff4
118 N99812801a15945b6bf1c2307b9a34ff4 rdf:first sg:person.01071206076.27
119 rdf:rest Nf05041d0cd4b45f4a369a92f2e90ee94
120 Na1f07a5c68474fca92a4192fb64095d7 rdf:first sg:person.01205434476.75
121 rdf:rest N3001031c7e3e48d1b9fee9d96634f153
122 Na6cc1d31044c422999cd0399719cc3a0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Cell Cycle Proteins
124 rdf:type schema:DefinedTerm
125 Nd2ae663536514717a9ff6e6290c86c77 schema:name Springer Nature - SN SciGraph project
126 rdf:type schema:Organization
127 Ndcd82a58ff39470abe0ccda0f17c0ff1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name DNA Replication
129 rdf:type schema:DefinedTerm
130 Ne8d87af3011a4cdda449a396231c2d24 schema:name nlm_unique_id
131 schema:value 0410462
132 rdf:type schema:PropertyValue
133 Nf05041d0cd4b45f4a369a92f2e90ee94 rdf:first sg:person.01355370016.67
134 rdf:rest Na1f07a5c68474fca92a4192fb64095d7
135 Nfabdd197f2d946b4984689ec9821bc9e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name DNA, Fungal
137 rdf:type schema:DefinedTerm
138 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
139 schema:name Biological Sciences
140 rdf:type schema:DefinedTerm
141 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
142 schema:name Biochemistry and Cell Biology
143 rdf:type schema:DefinedTerm
144 sg:journal.1018957 schema:issn 0090-0028
145 1476-4687
146 schema:name Nature
147 rdf:type schema:Periodical
148 sg:person.01071206076.27 schema:affiliation https://www.grid.ac/institutes/grid.419082.6
149 schema:familyName Umemori
150 schema:givenName Toshiko
151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01071206076.27
152 rdf:type schema:Person
153 sg:person.01205434476.75 schema:affiliation N6733d30ae1f0413886caf924a1466851
154 schema:familyName Muramatsu
155 schema:givenName Sachiko
156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01205434476.75
157 rdf:type schema:Person
158 sg:person.01321663076.98 schema:affiliation https://www.grid.ac/institutes/grid.419082.6
159 schema:familyName Kamimura
160 schema:givenName Yoichiro
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321663076.98
162 rdf:type schema:Person
163 sg:person.01355370016.67 schema:affiliation https://www.grid.ac/institutes/grid.419082.6
164 schema:familyName Hirai
165 schema:givenName Kazuyuki
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01355370016.67
167 rdf:type schema:Person
168 sg:person.015157102075.61 schema:affiliation https://www.grid.ac/institutes/grid.419082.6
169 schema:familyName Araki
170 schema:givenName Hiroyuki
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015157102075.61
172 rdf:type schema:Person
173 sg:person.0654321475.75 schema:affiliation https://www.grid.ac/institutes/grid.419082.6
174 schema:familyName Tanaka
175 schema:givenName Seiji
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0654321475.75
177 rdf:type schema:Person
178 sg:pub.10.1038/15649 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039277346
179 https://doi.org/10.1038/15649
180 rdf:type schema:CreativeWork
181 sg:pub.10.1038/35030148 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007129994
182 https://doi.org/10.1038/35030148
183 rdf:type schema:CreativeWork
184 sg:pub.10.1038/35082600 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036007845
185 https://doi.org/10.1038/35082600
186 rdf:type schema:CreativeWork
187 sg:pub.10.1038/nature05432 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017905987
188 https://doi.org/10.1038/nature05432
189 rdf:type schema:CreativeWork
190 sg:pub.10.1038/nature713 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049620350
191 https://doi.org/10.1038/nature713
192 rdf:type schema:CreativeWork
193 sg:pub.10.1038/ncb1382 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020448729
194 https://doi.org/10.1038/ncb1382
195 rdf:type schema:CreativeWork
196 sg:pub.10.1038/nrm1663 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012862888
197 https://doi.org/10.1038/nrm1663
198 rdf:type schema:CreativeWork
199 https://app.dimensions.ai/details/publication/pub.1083357036 schema:CreativeWork
200 https://doi.org/10.1002/bies.10370 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038377320
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1002/jcp.10070 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012453312
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1016/0092-8674(87)90642-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030825149
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1016/j.cub.2004.09.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007533389
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1016/j.dnarep.2005.04.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011349997
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1016/j.molcel.2006.01.030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047999181
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1016/j.tibs.2004.09.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015162467
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1016/s1097-2765(03)00441-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050158520
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1038/sj.emboj.7601063 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027987961
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1038/sj.emboj.7601075 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003172621
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1073/pnas.0602400103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009060164
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1073/pnas.92.25.11791 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009601194
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1093/emboj/20.8.2097 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010219249
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1101/gad.1065903 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046475412
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1128/mcb.18.10.6102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045383676
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1128/mcb.20.8.2809-2817.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035125901
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1128/mcb.23.8.2733-2748.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039698497
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1146/annurev.biochem.71.110601.135425 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040856314
235 rdf:type schema:CreativeWork
236 https://www.grid.ac/institutes/grid.419082.6 schema:alternateName Japan Science and Technology Agency
237 schema:name CREST, Kawaguchi, Saitama 332-0012, Japan
238 Department of Genetics, SOKENDAI, Yata 1111, Mishima, Shizuoka 411-8540, Japan
239 Division of Microbial Genetics, National Institute of Genetics, Research Organization of Information and Systems, and,
240 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...