Crystal structure of a protein phosphatase 2A heterotrimeric holoenzyme View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-01

AUTHORS

Uhn Soo Cho, Wenqing Xu

ABSTRACT

Protein phosphatase 2A (PP2A) is a principal Ser/Thr phosphatase, the deregulation of which is associated with multiple human cancers, Alzheimer's disease and increased susceptibility to pathogen infections. How PP2A is structurally organized and functionally regulated remains unclear. Here we report the crystal structure of an AB'C heterotrimeric PP2A holoenzyme. The structure reveals that the HEAT repeats of the scaffold A subunit form a horseshoe-shaped fold, holding the catalytic C and regulatory B' subunits together on the same side. The regulatory B' subunit forms pseudo-HEAT repeats and interacts with the C subunit near the active site, thereby defining substrate specificity. The methylated carboxy-terminal tail of the C subunit interacts with a highly negatively charged region at the interface between A and B' subunits, suggesting that the C-terminal carboxyl methylation of the C subunit promotes B' subunit recruitment by neutralizing charge repulsion. Together, our structural results establish a crucial foundation for understanding PP2A assembly, substrate recruitment and regulation. More... »

PAGES

53

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nature05351

DOI

http://dx.doi.org/10.1038/nature05351

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027078509

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17086192


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Binding Sites", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Catalysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Crystallography, X-Ray", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Holoenzymes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Methylation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphoprotein Phosphatases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Phosphatase 2", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Structure, Quaternary", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Subunits", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Substrate Specificity", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Washington", 
          "id": "https://www.grid.ac/institutes/grid.34477.33", 
          "name": [
            "Department of Biological Structure, University of Washington, Seattle, Washington 98195, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cho", 
        "givenName": "Uhn Soo", 
        "id": "sg:person.013650613627.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013650613627.51"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Washington", 
          "id": "https://www.grid.ac/institutes/grid.34477.33", 
          "name": [
            "Department of Biological Structure, University of Washington, Seattle, Washington 98195, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xu", 
        "givenName": "Wenqing", 
        "id": "sg:person.0657623161.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0657623161.58"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1093/emboj/19.21.5672", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000573338"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.pep.2005.06.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001483961"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0955-0674(99)00074-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002618028"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0076-6879(97)76066-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004907170"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jsbi.1999.4094", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005545483"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/19.4.562", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007655288"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.17.3.1692", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014834661"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1997.t01-1-00200.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014894781"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.12.11.4872", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017244907"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1535-6108(04)00026-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017377201"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s090744499801405x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019210052"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444904019158", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020281098"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m008694200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021308181"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.95.25.14693", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023715073"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0907444997018805", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026895546"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/sj.emboj.7600980", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028629832"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/sj.emboj.7600980", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028629832"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jnen/63.10.1080", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029346783"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/19.21.5682", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029752984"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj3530417", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033284032"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bj3530417", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033284032"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/376745a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035124605", 
          "https://doi.org/10.1038/376745a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0076-6879(97)76073-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035972019"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.12.1.185", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040286720"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1204059", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042334898", 
          "https://doi.org/10.1038/sj.onc.1204059"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1204059", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042334898", 
          "https://doi.org/10.1038/sj.onc.1204059"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.270.44.26123", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045295025"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-2952(98)00245-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045456533"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.bjc.6603317", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045720342", 
          "https://doi.org/10.1038/sj.bjc.6603317"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.bjc.6603317", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045720342", 
          "https://doi.org/10.1038/sj.bjc.6603317"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)80963-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046345009"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0898-6568(00)00123-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046751751"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1201259", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048808700", 
          "https://doi.org/10.1038/sj.onc.1201259"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1201259", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048808700", 
          "https://doi.org/10.1038/sj.onc.1201259"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.129.2.397", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050042092"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0065-2571(94)90017-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050406292"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0065-2571(94)90017-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050406292"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(95)90439-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051132304"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.gde.2004.12.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051323142"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/bbrc.1995.1334", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051832367"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/0264-6021:3530417", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056715265"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1325671", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062475372"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.282.5387.284", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062562774"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.283.5410.2089", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062564650"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/stke.2252004pe13", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062692047"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083359444", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2007-01", 
    "datePublishedReg": "2007-01-01", 
    "description": "Protein phosphatase 2A (PP2A) is a principal Ser/Thr phosphatase, the deregulation of which is associated with multiple human cancers, Alzheimer's disease and increased susceptibility to pathogen infections. How PP2A is structurally organized and functionally regulated remains unclear. Here we report the crystal structure of an AB'C heterotrimeric PP2A holoenzyme. The structure reveals that the HEAT repeats of the scaffold A subunit form a horseshoe-shaped fold, holding the catalytic C and regulatory B' subunits together on the same side. The regulatory B' subunit forms pseudo-HEAT repeats and interacts with the C subunit near the active site, thereby defining substrate specificity. The methylated carboxy-terminal tail of the C subunit interacts with a highly negatively charged region at the interface between A and B' subunits, suggesting that the C-terminal carboxyl methylation of the C subunit promotes B' subunit recruitment by neutralizing charge repulsion. Together, our structural results establish a crucial foundation for understanding PP2A assembly, substrate recruitment and regulation.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nature05351", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7123", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "445"
      }
    ], 
    "name": "Crystal structure of a protein phosphatase 2A heterotrimeric holoenzyme", 
    "pagination": "53", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "80fe5c542b76669a423a5a426cc9de52252cbdade48a69768e9e02204b200173"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "17086192"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nature05351"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1027078509"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nature05351", 
      "https://app.dimensions.ai/details/publication/pub.1027078509"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:10", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000361_0000000361/records_53977_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/nature05351"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature05351'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature05351'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature05351'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature05351'


 

This table displays all metadata directly associated to this object as RDF triples.

247 TRIPLES      21 PREDICATES      81 URIs      33 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nature05351 schema:about N3264f01aa5e84826b8a0fb3d26b1c085
2 N4bff952fc1214a7b9fb2470539b25116
3 N4c9877793f5e4b709cf7dbeffee088d0
4 N6c5685ed7442424eab51664715bb8db9
5 N7422f44cc5644d2e8ef5176bd787f01d
6 N80df1819a84342fbaaf7f22d68be68ec
7 N973b67b2422446d3b9334c12a47bcc27
8 Na72bea762f7442c4812eef3e4bf9709c
9 Nd8acd7d99cff48c79e0b50741762f5ba
10 Ne0615e50ae534fc8bc1ea30cf32d91b0
11 Ne5ccebc1b7124b0cabe1008c1283a308
12 Nf0f53bea76dc4aa1ac356afce014a719
13 anzsrc-for:06
14 anzsrc-for:0601
15 schema:author Ne79ec8de5bae4e81a868d1521a3e7b6e
16 schema:citation sg:pub.10.1038/376745a0
17 sg:pub.10.1038/sj.bjc.6603317
18 sg:pub.10.1038/sj.onc.1201259
19 sg:pub.10.1038/sj.onc.1204059
20 https://app.dimensions.ai/details/publication/pub.1083359444
21 https://doi.org/10.1006/bbrc.1995.1334
22 https://doi.org/10.1006/jsbi.1999.4094
23 https://doi.org/10.1016/0065-2571(94)90017-5
24 https://doi.org/10.1016/0092-8674(95)90439-5
25 https://doi.org/10.1016/j.gde.2004.12.004
26 https://doi.org/10.1016/j.pep.2005.06.002
27 https://doi.org/10.1016/s0006-2952(98)00245-7
28 https://doi.org/10.1016/s0076-6879(97)76066-x
29 https://doi.org/10.1016/s0076-6879(97)76073-7
30 https://doi.org/10.1016/s0092-8674(00)80963-0
31 https://doi.org/10.1016/s0898-6568(00)00123-6
32 https://doi.org/10.1016/s0955-0674(99)00074-5
33 https://doi.org/10.1016/s1535-6108(04)00026-1
34 https://doi.org/10.1038/sj.emboj.7600980
35 https://doi.org/10.1042/0264-6021:3530417
36 https://doi.org/10.1042/bj3530417
37 https://doi.org/10.1073/pnas.95.25.14693
38 https://doi.org/10.1074/jbc.270.44.26123
39 https://doi.org/10.1074/jbc.m008694200
40 https://doi.org/10.1083/jcb.129.2.397
41 https://doi.org/10.1091/mbc.12.1.185
42 https://doi.org/10.1093/emboj/19.21.5672
43 https://doi.org/10.1093/emboj/19.21.5682
44 https://doi.org/10.1093/emboj/19.4.562
45 https://doi.org/10.1093/jnen/63.10.1080
46 https://doi.org/10.1107/s0907444904019158
47 https://doi.org/10.1107/s0907444997018805
48 https://doi.org/10.1107/s090744499801405x
49 https://doi.org/10.1111/j.1432-1033.1997.t01-1-00200.x
50 https://doi.org/10.1126/science.1325671
51 https://doi.org/10.1126/science.282.5387.284
52 https://doi.org/10.1126/science.283.5410.2089
53 https://doi.org/10.1126/stke.2252004pe13
54 https://doi.org/10.1128/mcb.12.11.4872
55 https://doi.org/10.1128/mcb.17.3.1692
56 schema:datePublished 2007-01
57 schema:datePublishedReg 2007-01-01
58 schema:description Protein phosphatase 2A (PP2A) is a principal Ser/Thr phosphatase, the deregulation of which is associated with multiple human cancers, Alzheimer's disease and increased susceptibility to pathogen infections. How PP2A is structurally organized and functionally regulated remains unclear. Here we report the crystal structure of an AB'C heterotrimeric PP2A holoenzyme. The structure reveals that the HEAT repeats of the scaffold A subunit form a horseshoe-shaped fold, holding the catalytic C and regulatory B' subunits together on the same side. The regulatory B' subunit forms pseudo-HEAT repeats and interacts with the C subunit near the active site, thereby defining substrate specificity. The methylated carboxy-terminal tail of the C subunit interacts with a highly negatively charged region at the interface between A and B' subunits, suggesting that the C-terminal carboxyl methylation of the C subunit promotes B' subunit recruitment by neutralizing charge repulsion. Together, our structural results establish a crucial foundation for understanding PP2A assembly, substrate recruitment and regulation.
59 schema:genre research_article
60 schema:inLanguage en
61 schema:isAccessibleForFree false
62 schema:isPartOf Nb01d32a9de40452fa2fd9b08c7ba4f08
63 Nbd129d13de0d488dbb97463ffe8ac764
64 sg:journal.1018957
65 schema:name Crystal structure of a protein phosphatase 2A heterotrimeric holoenzyme
66 schema:pagination 53
67 schema:productId N007df1da2c7f4559a1744ae8a7088672
68 N413de81819ad4da58728d8adbeb16bf6
69 N9042ba71f4b74e44a3ed12e5c673bd19
70 Na40f1a1398294e8cbb108e986a7a5150
71 Naac9d1b1bded4c19b4a3f789721763fe
72 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027078509
73 https://doi.org/10.1038/nature05351
74 schema:sdDatePublished 2019-04-11T12:10
75 schema:sdLicense https://scigraph.springernature.com/explorer/license/
76 schema:sdPublisher Nad5fe2aac94d4f9a96d7f3b8572fd6f9
77 schema:url https://www.nature.com/articles/nature05351
78 sgo:license sg:explorer/license/
79 sgo:sdDataset articles
80 rdf:type schema:ScholarlyArticle
81 N007df1da2c7f4559a1744ae8a7088672 schema:name readcube_id
82 schema:value 80fe5c542b76669a423a5a426cc9de52252cbdade48a69768e9e02204b200173
83 rdf:type schema:PropertyValue
84 N0dafa398a9e848ba821cc739fa87a26a rdf:first sg:person.0657623161.58
85 rdf:rest rdf:nil
86 N3264f01aa5e84826b8a0fb3d26b1c085 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Catalysis
88 rdf:type schema:DefinedTerm
89 N413de81819ad4da58728d8adbeb16bf6 schema:name doi
90 schema:value 10.1038/nature05351
91 rdf:type schema:PropertyValue
92 N4bff952fc1214a7b9fb2470539b25116 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Protein Phosphatase 2
94 rdf:type schema:DefinedTerm
95 N4c9877793f5e4b709cf7dbeffee088d0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Phosphoprotein Phosphatases
97 rdf:type schema:DefinedTerm
98 N6c5685ed7442424eab51664715bb8db9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Protein Subunits
100 rdf:type schema:DefinedTerm
101 N7422f44cc5644d2e8ef5176bd787f01d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Methylation
103 rdf:type schema:DefinedTerm
104 N80df1819a84342fbaaf7f22d68be68ec schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Holoenzymes
106 rdf:type schema:DefinedTerm
107 N9042ba71f4b74e44a3ed12e5c673bd19 schema:name pubmed_id
108 schema:value 17086192
109 rdf:type schema:PropertyValue
110 N973b67b2422446d3b9334c12a47bcc27 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Crystallography, X-Ray
112 rdf:type schema:DefinedTerm
113 Na40f1a1398294e8cbb108e986a7a5150 schema:name dimensions_id
114 schema:value pub.1027078509
115 rdf:type schema:PropertyValue
116 Na72bea762f7442c4812eef3e4bf9709c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Substrate Specificity
118 rdf:type schema:DefinedTerm
119 Naac9d1b1bded4c19b4a3f789721763fe schema:name nlm_unique_id
120 schema:value 0410462
121 rdf:type schema:PropertyValue
122 Nad5fe2aac94d4f9a96d7f3b8572fd6f9 schema:name Springer Nature - SN SciGraph project
123 rdf:type schema:Organization
124 Nb01d32a9de40452fa2fd9b08c7ba4f08 schema:volumeNumber 445
125 rdf:type schema:PublicationVolume
126 Nbd129d13de0d488dbb97463ffe8ac764 schema:issueNumber 7123
127 rdf:type schema:PublicationIssue
128 Nd8acd7d99cff48c79e0b50741762f5ba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Humans
130 rdf:type schema:DefinedTerm
131 Ne0615e50ae534fc8bc1ea30cf32d91b0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Binding Sites
133 rdf:type schema:DefinedTerm
134 Ne5ccebc1b7124b0cabe1008c1283a308 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Models, Molecular
136 rdf:type schema:DefinedTerm
137 Ne79ec8de5bae4e81a868d1521a3e7b6e rdf:first sg:person.013650613627.51
138 rdf:rest N0dafa398a9e848ba821cc739fa87a26a
139 Nf0f53bea76dc4aa1ac356afce014a719 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Protein Structure, Quaternary
141 rdf:type schema:DefinedTerm
142 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
143 schema:name Biological Sciences
144 rdf:type schema:DefinedTerm
145 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
146 schema:name Biochemistry and Cell Biology
147 rdf:type schema:DefinedTerm
148 sg:journal.1018957 schema:issn 0090-0028
149 1476-4687
150 schema:name Nature
151 rdf:type schema:Periodical
152 sg:person.013650613627.51 schema:affiliation https://www.grid.ac/institutes/grid.34477.33
153 schema:familyName Cho
154 schema:givenName Uhn Soo
155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013650613627.51
156 rdf:type schema:Person
157 sg:person.0657623161.58 schema:affiliation https://www.grid.ac/institutes/grid.34477.33
158 schema:familyName Xu
159 schema:givenName Wenqing
160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0657623161.58
161 rdf:type schema:Person
162 sg:pub.10.1038/376745a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035124605
163 https://doi.org/10.1038/376745a0
164 rdf:type schema:CreativeWork
165 sg:pub.10.1038/sj.bjc.6603317 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045720342
166 https://doi.org/10.1038/sj.bjc.6603317
167 rdf:type schema:CreativeWork
168 sg:pub.10.1038/sj.onc.1201259 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048808700
169 https://doi.org/10.1038/sj.onc.1201259
170 rdf:type schema:CreativeWork
171 sg:pub.10.1038/sj.onc.1204059 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042334898
172 https://doi.org/10.1038/sj.onc.1204059
173 rdf:type schema:CreativeWork
174 https://app.dimensions.ai/details/publication/pub.1083359444 schema:CreativeWork
175 https://doi.org/10.1006/bbrc.1995.1334 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051832367
176 rdf:type schema:CreativeWork
177 https://doi.org/10.1006/jsbi.1999.4094 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005545483
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1016/0065-2571(94)90017-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050406292
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1016/0092-8674(95)90439-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051132304
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1016/j.gde.2004.12.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051323142
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1016/j.pep.2005.06.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001483961
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1016/s0006-2952(98)00245-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045456533
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1016/s0076-6879(97)76066-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1004907170
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1016/s0076-6879(97)76073-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035972019
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1016/s0092-8674(00)80963-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046345009
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1016/s0898-6568(00)00123-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046751751
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1016/s0955-0674(99)00074-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002618028
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1016/s1535-6108(04)00026-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017377201
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1038/sj.emboj.7600980 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028629832
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1042/0264-6021:3530417 schema:sameAs https://app.dimensions.ai/details/publication/pub.1056715265
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1042/bj3530417 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033284032
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1073/pnas.95.25.14693 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023715073
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1074/jbc.270.44.26123 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045295025
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1074/jbc.m008694200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021308181
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1083/jcb.129.2.397 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050042092
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1091/mbc.12.1.185 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040286720
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1093/emboj/19.21.5672 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000573338
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1093/emboj/19.21.5682 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029752984
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1093/emboj/19.4.562 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007655288
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1093/jnen/63.10.1080 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029346783
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1107/s0907444904019158 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020281098
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1107/s0907444997018805 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026895546
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1107/s090744499801405x schema:sameAs https://app.dimensions.ai/details/publication/pub.1019210052
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1111/j.1432-1033.1997.t01-1-00200.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1014894781
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1126/science.1325671 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062475372
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1126/science.282.5387.284 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062562774
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1126/science.283.5410.2089 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062564650
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1126/stke.2252004pe13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062692047
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1128/mcb.12.11.4872 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017244907
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1128/mcb.17.3.1692 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014834661
244 rdf:type schema:CreativeWork
245 https://www.grid.ac/institutes/grid.34477.33 schema:alternateName University of Washington
246 schema:name Department of Biological Structure, University of Washington, Seattle, Washington 98195, USA
247 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...