Dynamics of heat shock factor association with native gene loci in living cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-08

AUTHORS

Jie Yao, Katherine M Munson, Watt W Webb, John T Lis

ABSTRACT

Direct observation of transcription factor action in the living cell nucleus can provide important insights into gene regulatory mechanisms. Live-cell imaging techniques have enabled the visualization of a variety of intranuclear activities, from chromosome dynamics to gene expression. However, progress in studying transcription regulation of specific native genes has been limited, primarily as a result of difficulties in resolving individual gene loci and in detecting the small number of protein molecules functioning within active transcription units. Here we report that multiphoton microscopy imaging of polytene nuclei in living Drosophila salivary glands allows real-time analysis of transcription factor recruitment and exchange on specific native genes. After heat shock, we have visualized the recruitment of RNA polymerase II (Pol II) to native hsp70 gene loci 87A and 87C in real time. We show that heat shock factor (HSF), the transcription activator of hsp70, is localized to the nucleus before heat shock and translocates from nucleoplasm to chromosomal loci after heat shock. Assays based on fluorescence recovery after photobleaching show a rapid exchange of HSF at chromosomal loci under non-heat-shock conditions but a very slow exchange after heat shock. However, this is not a consequence of a change of HSF diffusibility, as shown here directly by fluorescence correlation spectroscopy. Our results provide strong evidence that activated HSF is stably bound to DNA in vivo and that turnover or disassembly of transcription activator is not required for rounds of hsp70 transcription. This and previous studies indicate that transcription activators display diverse dynamic behaviours in their associations with targeted loci in living cells. Our method can be applied to study the dynamics of many factors involved in transcription and RNA processing, and in their regulation at native heat shock genes in vivo. More... »

PAGES

1050-1053

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nature05025

DOI

http://dx.doi.org/10.1038/nature05025

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045553671

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16929308


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Survival", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromosomes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drosophila", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drosophila Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Insect", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Heat-Shock Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Heat-Shock Response", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kinetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA Polymerase II", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Salivary Glands", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transcription, Genetic", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Cornell University", 
          "id": "https://www.grid.ac/institutes/grid.5386.8", 
          "name": [
            "Field of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yao", 
        "givenName": "Jie", 
        "id": "sg:person.01044320207.50", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01044320207.50"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Munson", 
        "givenName": "Katherine M", 
        "id": "sg:person.01172060721.65", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01172060721.65"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Webb", 
        "givenName": "Watt W", 
        "id": "sg:person.0614271700.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0614271700.09"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Lis", 
        "givenName": "John T", 
        "id": "sg:person.0647743124.75", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0647743124.75"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/302676a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000696901", 
          "https://doi.org/10.1038/302676a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/302676a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000696901", 
          "https://doi.org/10.1038/302676a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.cb.11.110195.002301", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004127070"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0959-440x(02)00294-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005454112"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.13.6.3456", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008676022"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(04)00171-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013063905"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.7.12a.2329", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014891809"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.287.5456.1262", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015504530"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.biochem.72.121801.161724", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017161034"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.molcel.2005.04.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018024583"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1073051", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018899674"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm1494", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018927250", 
          "https://doi.org/10.1038/nrm1494"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm1494", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018927250", 
          "https://doi.org/10.1038/nrm1494"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0076-6879(03)76026-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026836991"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.24.7.2682-2697.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030104507"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.11.10.1299", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032824495"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/353822a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034128417", 
          "https://doi.org/10.1038/353822a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt899", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034655204", 
          "https://doi.org/10.1038/nbt899"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt899", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034655204", 
          "https://doi.org/10.1038/nbt899"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04098", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038037436", 
          "https://doi.org/10.1038/nature04098"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04098", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038037436", 
          "https://doi.org/10.1038/nature04098"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature04098", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038037436", 
          "https://doi.org/10.1038/nature04098"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.25.20.9092-9102.2005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041789578"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2004.12.025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041832856"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.200303200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042416880"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-3495(76)85755-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046177476"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm976", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046935422", 
          "https://doi.org/10.1038/nrm976"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrm976", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046935422", 
          "https://doi.org/10.1038/nrm976"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0962-8924(01)02000-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047210244"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05067", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048978888", 
          "https://doi.org/10.1038/nature05067"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05067", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048978888", 
          "https://doi.org/10.1038/nature05067"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature05067", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048978888", 
          "https://doi.org/10.1038/nature05067"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/sj.emboj.7600977", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050769067"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/sj.emboj.7600977", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050769067"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/sqb.1998.63.347", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060415644"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physrevlett.29.705", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060776513"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1103/physrevlett.29.705", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060776513"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2321027", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062532641"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.291.5505.843", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062572878"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/oxfordjournals.jhered.a104022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083617386"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2006-08", 
    "datePublishedReg": "2006-08-01", 
    "description": "Direct observation of transcription factor action in the living cell nucleus can provide important insights into gene regulatory mechanisms. Live-cell imaging techniques have enabled the visualization of a variety of intranuclear activities, from chromosome dynamics to gene expression. However, progress in studying transcription regulation of specific native genes has been limited, primarily as a result of difficulties in resolving individual gene loci and in detecting the small number of protein molecules functioning within active transcription units. Here we report that multiphoton microscopy imaging of polytene nuclei in living Drosophila salivary glands allows real-time analysis of transcription factor recruitment and exchange on specific native genes. After heat shock, we have visualized the recruitment of RNA polymerase II (Pol II) to native hsp70 gene loci 87A and 87C in real time. We show that heat shock factor (HSF), the transcription activator of hsp70, is localized to the nucleus before heat shock and translocates from nucleoplasm to chromosomal loci after heat shock. Assays based on fluorescence recovery after photobleaching show a rapid exchange of HSF at chromosomal loci under non-heat-shock conditions but a very slow exchange after heat shock. However, this is not a consequence of a change of HSF diffusibility, as shown here directly by fluorescence correlation spectroscopy. Our results provide strong evidence that activated HSF is stably bound to DNA in vivo and that turnover or disassembly of transcription activator is not required for rounds of hsp70 transcription. This and previous studies indicate that transcription activators display diverse dynamic behaviours in their associations with targeted loci in living cells. Our method can be applied to study the dynamics of many factors involved in transcription and RNA processing, and in their regulation at native heat shock genes in vivo.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nature05025", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7106", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "442"
      }
    ], 
    "name": "Dynamics of heat shock factor association with native gene loci in living cells", 
    "pagination": "1050-1053", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "d8fe0af1fbf209eb536fe2bb403fa9b24fb8c492755500d0161e02780d3bb3ed"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "16929308"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nature05025"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1045553671"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nature05025", 
      "https://app.dimensions.ai/details/publication/pub.1045553671"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:00", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000365_0000000365/records_71707_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/nature/journal/v442/n7106/full/nature05025.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature05025'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature05025'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature05025'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature05025'


 

This table displays all metadata directly associated to this object as RDF triples.

236 TRIPLES      21 PREDICATES      72 URIs      34 LITERALS      22 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nature05025 schema:about N01b09618e8644b4c98d71052bc240979
2 N14fb53326b304990ba9e98d01cb5b17b
3 N26bcf8ecc11f4b51a53e5e09f8885d3e
4 N2c0b1b70048d4c32a550d059628b9157
5 N6e9548a3788c4e318c23485fa1d06556
6 N730b4935858b4697ab96d426e25f4086
7 N78eeffec2f0448ef83aa57b343c77ee0
8 N88c8f54ca4a048b3b7b885899ed51116
9 N991596c9029d40c1884e3df3727fb768
10 N998661c6a36e471e941a26f17cec29f6
11 Nad5e09180a8d40caab38218ab40b8214
12 Nae531ed77d1e4e3798d1f489467aba1e
13 Nc941e859590f4ecc9bb24d53d155a086
14 anzsrc-for:06
15 anzsrc-for:0604
16 schema:author N839f0af722e44683ad04e122c93abf47
17 schema:citation sg:pub.10.1038/302676a0
18 sg:pub.10.1038/353822a0
19 sg:pub.10.1038/nature04098
20 sg:pub.10.1038/nature05067
21 sg:pub.10.1038/nbt899
22 sg:pub.10.1038/nrm1494
23 sg:pub.10.1038/nrm976
24 https://doi.org/10.1016/j.cell.2004.12.025
25 https://doi.org/10.1016/j.molcel.2005.04.017
26 https://doi.org/10.1016/s0006-3495(76)85755-4
27 https://doi.org/10.1016/s0076-6879(03)76026-1
28 https://doi.org/10.1016/s0092-8674(04)00171-0
29 https://doi.org/10.1016/s0959-440x(02)00294-4
30 https://doi.org/10.1016/s0962-8924(01)02000-1
31 https://doi.org/10.1038/sj.emboj.7600977
32 https://doi.org/10.1083/jcb.200303200
33 https://doi.org/10.1093/oxfordjournals.jhered.a104022
34 https://doi.org/10.1101/gad.11.10.1299
35 https://doi.org/10.1101/gad.7.12a.2329
36 https://doi.org/10.1101/sqb.1998.63.347
37 https://doi.org/10.1103/physrevlett.29.705
38 https://doi.org/10.1126/science.1073051
39 https://doi.org/10.1126/science.2321027
40 https://doi.org/10.1126/science.287.5456.1262
41 https://doi.org/10.1126/science.291.5505.843
42 https://doi.org/10.1128/mcb.13.6.3456
43 https://doi.org/10.1128/mcb.24.7.2682-2697.2004
44 https://doi.org/10.1128/mcb.25.20.9092-9102.2005
45 https://doi.org/10.1146/annurev.biochem.72.121801.161724
46 https://doi.org/10.1146/annurev.cb.11.110195.002301
47 schema:datePublished 2006-08
48 schema:datePublishedReg 2006-08-01
49 schema:description Direct observation of transcription factor action in the living cell nucleus can provide important insights into gene regulatory mechanisms. Live-cell imaging techniques have enabled the visualization of a variety of intranuclear activities, from chromosome dynamics to gene expression. However, progress in studying transcription regulation of specific native genes has been limited, primarily as a result of difficulties in resolving individual gene loci and in detecting the small number of protein molecules functioning within active transcription units. Here we report that multiphoton microscopy imaging of polytene nuclei in living Drosophila salivary glands allows real-time analysis of transcription factor recruitment and exchange on specific native genes. After heat shock, we have visualized the recruitment of RNA polymerase II (Pol II) to native hsp70 gene loci 87A and 87C in real time. We show that heat shock factor (HSF), the transcription activator of hsp70, is localized to the nucleus before heat shock and translocates from nucleoplasm to chromosomal loci after heat shock. Assays based on fluorescence recovery after photobleaching show a rapid exchange of HSF at chromosomal loci under non-heat-shock conditions but a very slow exchange after heat shock. However, this is not a consequence of a change of HSF diffusibility, as shown here directly by fluorescence correlation spectroscopy. Our results provide strong evidence that activated HSF is stably bound to DNA in vivo and that turnover or disassembly of transcription activator is not required for rounds of hsp70 transcription. This and previous studies indicate that transcription activators display diverse dynamic behaviours in their associations with targeted loci in living cells. Our method can be applied to study the dynamics of many factors involved in transcription and RNA processing, and in their regulation at native heat shock genes in vivo.
50 schema:genre research_article
51 schema:inLanguage en
52 schema:isAccessibleForFree false
53 schema:isPartOf Nf1b8baac893a406c9548b295e41ecc96
54 Nfd018253a6ab4d10b776efa1091d6d8a
55 sg:journal.1018957
56 schema:name Dynamics of heat shock factor association with native gene loci in living cells
57 schema:pagination 1050-1053
58 schema:productId N11fa673ec5f24a79b73ecc62dc0b1e57
59 N8540de92abee45f29027f19223f8d461
60 N8963090008674261be503cfe522dd872
61 Nd655035ab6624940b5f351526982f7bb
62 Ndf6ba6834b694ec9b4ea9596597109d8
63 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045553671
64 https://doi.org/10.1038/nature05025
65 schema:sdDatePublished 2019-04-11T13:00
66 schema:sdLicense https://scigraph.springernature.com/explorer/license/
67 schema:sdPublisher N798c30ef2c004b4c86a3be92005506be
68 schema:url http://www.nature.com/nature/journal/v442/n7106/full/nature05025.html
69 sgo:license sg:explorer/license/
70 sgo:sdDataset articles
71 rdf:type schema:ScholarlyArticle
72 N01b09618e8644b4c98d71052bc240979 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
73 schema:name Kinetics
74 rdf:type schema:DefinedTerm
75 N11fa673ec5f24a79b73ecc62dc0b1e57 schema:name nlm_unique_id
76 schema:value 0410462
77 rdf:type schema:PropertyValue
78 N14fb53326b304990ba9e98d01cb5b17b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Heat-Shock Response
80 rdf:type schema:DefinedTerm
81 N1b631dc4f6db47e68f445849db26a930 rdf:first sg:person.01172060721.65
82 rdf:rest Nb03f22aea0b7416492c8eacffdd7272c
83 N26bcf8ecc11f4b51a53e5e09f8885d3e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Drosophila
85 rdf:type schema:DefinedTerm
86 N2c0b1b70048d4c32a550d059628b9157 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Salivary Glands
88 rdf:type schema:DefinedTerm
89 N6e9548a3788c4e318c23485fa1d06556 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Animals
91 rdf:type schema:DefinedTerm
92 N730b4935858b4697ab96d426e25f4086 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Gene Expression Regulation
94 rdf:type schema:DefinedTerm
95 N78eeffec2f0448ef83aa57b343c77ee0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name RNA Polymerase II
97 rdf:type schema:DefinedTerm
98 N798c30ef2c004b4c86a3be92005506be schema:name Springer Nature - SN SciGraph project
99 rdf:type schema:Organization
100 N839f0af722e44683ad04e122c93abf47 rdf:first sg:person.01044320207.50
101 rdf:rest N1b631dc4f6db47e68f445849db26a930
102 N8540de92abee45f29027f19223f8d461 schema:name pubmed_id
103 schema:value 16929308
104 rdf:type schema:PropertyValue
105 N88c8f54ca4a048b3b7b885899ed51116 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Genes, Insect
107 rdf:type schema:DefinedTerm
108 N8963090008674261be503cfe522dd872 schema:name readcube_id
109 schema:value d8fe0af1fbf209eb536fe2bb403fa9b24fb8c492755500d0161e02780d3bb3ed
110 rdf:type schema:PropertyValue
111 N991596c9029d40c1884e3df3727fb768 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Drosophila Proteins
113 rdf:type schema:DefinedTerm
114 N998661c6a36e471e941a26f17cec29f6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Chromosomes
116 rdf:type schema:DefinedTerm
117 Nad5e09180a8d40caab38218ab40b8214 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Heat-Shock Proteins
119 rdf:type schema:DefinedTerm
120 Nae531ed77d1e4e3798d1f489467aba1e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Transcription, Genetic
122 rdf:type schema:DefinedTerm
123 Nb03f22aea0b7416492c8eacffdd7272c rdf:first sg:person.0614271700.09
124 rdf:rest Nde73d83a0c834ce3a7fd9542e9a798b4
125 Nc941e859590f4ecc9bb24d53d155a086 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Cell Survival
127 rdf:type schema:DefinedTerm
128 Nd655035ab6624940b5f351526982f7bb schema:name doi
129 schema:value 10.1038/nature05025
130 rdf:type schema:PropertyValue
131 Nde73d83a0c834ce3a7fd9542e9a798b4 rdf:first sg:person.0647743124.75
132 rdf:rest rdf:nil
133 Ndf6ba6834b694ec9b4ea9596597109d8 schema:name dimensions_id
134 schema:value pub.1045553671
135 rdf:type schema:PropertyValue
136 Nf1b8baac893a406c9548b295e41ecc96 schema:volumeNumber 442
137 rdf:type schema:PublicationVolume
138 Nfd018253a6ab4d10b776efa1091d6d8a schema:issueNumber 7106
139 rdf:type schema:PublicationIssue
140 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
141 schema:name Biological Sciences
142 rdf:type schema:DefinedTerm
143 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
144 schema:name Genetics
145 rdf:type schema:DefinedTerm
146 sg:journal.1018957 schema:issn 0090-0028
147 1476-4687
148 schema:name Nature
149 rdf:type schema:Periodical
150 sg:person.01044320207.50 schema:affiliation https://www.grid.ac/institutes/grid.5386.8
151 schema:familyName Yao
152 schema:givenName Jie
153 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01044320207.50
154 rdf:type schema:Person
155 sg:person.01172060721.65 schema:familyName Munson
156 schema:givenName Katherine M
157 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01172060721.65
158 rdf:type schema:Person
159 sg:person.0614271700.09 schema:familyName Webb
160 schema:givenName Watt W
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0614271700.09
162 rdf:type schema:Person
163 sg:person.0647743124.75 schema:familyName Lis
164 schema:givenName John T
165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0647743124.75
166 rdf:type schema:Person
167 sg:pub.10.1038/302676a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000696901
168 https://doi.org/10.1038/302676a0
169 rdf:type schema:CreativeWork
170 sg:pub.10.1038/353822a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034128417
171 https://doi.org/10.1038/353822a0
172 rdf:type schema:CreativeWork
173 sg:pub.10.1038/nature04098 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038037436
174 https://doi.org/10.1038/nature04098
175 rdf:type schema:CreativeWork
176 sg:pub.10.1038/nature05067 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048978888
177 https://doi.org/10.1038/nature05067
178 rdf:type schema:CreativeWork
179 sg:pub.10.1038/nbt899 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034655204
180 https://doi.org/10.1038/nbt899
181 rdf:type schema:CreativeWork
182 sg:pub.10.1038/nrm1494 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018927250
183 https://doi.org/10.1038/nrm1494
184 rdf:type schema:CreativeWork
185 sg:pub.10.1038/nrm976 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046935422
186 https://doi.org/10.1038/nrm976
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1016/j.cell.2004.12.025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041832856
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1016/j.molcel.2005.04.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018024583
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1016/s0006-3495(76)85755-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046177476
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1016/s0076-6879(03)76026-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026836991
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1016/s0092-8674(04)00171-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013063905
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1016/s0959-440x(02)00294-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005454112
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1016/s0962-8924(01)02000-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047210244
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1038/sj.emboj.7600977 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050769067
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1083/jcb.200303200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042416880
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1093/oxfordjournals.jhered.a104022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083617386
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1101/gad.11.10.1299 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032824495
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1101/gad.7.12a.2329 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014891809
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1101/sqb.1998.63.347 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060415644
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1103/physrevlett.29.705 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060776513
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1126/science.1073051 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018899674
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1126/science.2321027 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062532641
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1126/science.287.5456.1262 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015504530
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1126/science.291.5505.843 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062572878
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1128/mcb.13.6.3456 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008676022
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1128/mcb.24.7.2682-2697.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030104507
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1128/mcb.25.20.9092-9102.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041789578
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1146/annurev.biochem.72.121801.161724 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017161034
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1146/annurev.cb.11.110195.002301 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004127070
233 rdf:type schema:CreativeWork
234 https://www.grid.ac/institutes/grid.5386.8 schema:alternateName Cornell University
235 schema:name Field of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA.
236 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...