Shugoshin collaborates with protein phosphatase 2A to protect cohesin View Full Text


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Article Info

DATE

2006-05

AUTHORS

Tomoya S. Kitajima, Takeshi Sakuno, Kei-ichiro Ishiguro, Shun-ichiro Iemura, Tohru Natsume, Shigehiro A. Kawashima, Yoshinori Watanabe

ABSTRACT

Sister chromatid cohesion, mediated by a complex called cohesin, is crucial--particularly at centromeres--for proper chromosome segregation in mitosis and meiosis. In animal mitotic cells, phosphorylation of cohesin promotes its dissociation from chromosomes, but centromeric cohesin is protected by shugoshin until kinetochores are properly captured by the spindle microtubules. However, the mechanism of shugoshin-dependent protection of cohesin is unknown. Here we find a specific subtype of serine/threonine protein phosphatase 2A (PP2A) associating with human shugoshin. PP2A colocalizes with shugoshin at centromeres and is required for centromeric protection. Purified shugoshin complex has an ability to reverse the phosphorylation of cohesin in vitro, suggesting that dephosphorylation of cohesin is the mechanism of protection at centromeres. Meiotic shugoshin of fission yeast also associates with PP2A, with both proteins collaboratively protecting Rec8-containing cohesin at centromeres. Thus, we have revealed a conserved mechanism of centromeric protection of eukaryotic chromosomes in mitosis and meiosis. More... »

PAGES

46-52

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nature04663

DOI

http://dx.doi.org/10.1038/nature04663

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034816995

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16541025


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