Purification and unique properties of mammary epithelial stem cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-01-04

AUTHORS

John Stingl, Peter Eirew, Ian Ricketson, Mark Shackleton, François Vaillant, David Choi, Haiyan I. Li, Connie J. Eaves

ABSTRACT

Elucidation of the cellular and molecular mechanisms that maintain mammary epithelial tissue integrity is of broad interest and paramount to the design of more effective treatments for breast cancer1. Evidence from both in vitro and in vivo experiments suggests that mammary cell differentiation is a hierarchical process originating in an uncommitted stem cell with self-renewal potential2,3,4. However, analysis of the properties and regulation of mammary stem cells has been limited by a lack of methods for their prospective isolation. Here we report the use of multi-parameter cell sorting and limiting dilution transplant analysis to demonstrate the purification of a rare subset of adult mouse mammary cells that are able individually to regenerate an entire mammary gland within 6 weeks in vivo while simultaneously executing up to ten symmetrical self-renewal divisions. These mammary stem cells are phenotypically distinct from and give rise to mammary epithelial progenitor cells that produce adherent colonies in vitro. The mammary stem cells are also a rapidly cycling population in the normal adult and have molecular features indicative of a basal position in the mammary epithelium. More... »

PAGES

993-997

References to SciGraph publications

Journal

TITLE

Nature

ISSUE

7079

VOLUME

439

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nature04496

    DOI

    http://dx.doi.org/10.1038/nature04496

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1040185730

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/16395311


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