Genome sequencing in microfabricated high-density picolitre reactors View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2005-09

AUTHORS

Marcel Margulies, Michael Egholm, William E. Altman, Said Attiya, Joel S. Bader, Lisa A. Bemben, Jan Berka, Michael S. Braverman, Yi-Ju Chen, Zhoutao Chen, Scott B. Dewell, Lei Du, Joseph M. Fierro, Xavier V. Gomes, Brian C. Godwin, Wen He, Scott Helgesen, Chun He Ho, Gerard P. Irzyk, Szilveszter C. Jando, Maria L. I. Alenquer, Thomas P. Jarvie, Kshama B. Jirage, Jong-Bum Kim, James R. Knight, Janna R. Lanza, John H. Leamon, Steven M. Lefkowitz, Ming Lei, Jing Li, Kenton L. Lohman, Hong Lu, Vinod B. Makhijani, Keith E. McDade, Michael P. McKenna, Eugene W. Myers, Elizabeth Nickerson, John R. Nobile, Ramona Plant, Bernard P. Puc, Michael T. Ronan, George T. Roth, Gary J. Sarkis, Jan Fredrik Simons, John W. Simpson, Maithreyan Srinivasan, Karrie R. Tartaro, Alexander Tomasz, Kari A. Vogt, Greg A. Volkmer, Shally H. Wang, Yong Wang, Michael P. Weiner, Pengguang Yu, Richard F. Begley, Jonathan M. Rothberg

ABSTRACT

The proliferation of large-scale DNA-sequencing projects in recent years has driven a search for alternative methods to reduce time and cost. Here we describe a scalable, highly parallel sequencing system with raw throughput significantly greater than that of state-of-the-art capillary electrophoresis instruments. The apparatus uses a novel fibre-optic slide of individual wells and is able to sequence 25 million bases, at 99% or better accuracy, in one four-hour run. To achieve an approximately 100-fold increase in throughput over current Sanger sequencing technology, we have developed an emulsion method for DNA amplification and an instrument for sequencing by synthesis using a pyrosequencing protocol optimized for solid support and picolitre-scale volumes. Here we show the utility, throughput, accuracy and robustness of this system by shotgun sequencing and de novo assembly of the Mycoplasma genitalium genome with 96% coverage at 99.96% accuracy in one run of the machine. More... »

PAGES

376

References to SciGraph publications

Journal

TITLE

Nature

ISSUE

7057

VOLUME

437

Related Patents

  • Efficient Arrays Of Amplified Polynucleotides
  • Novel Macrocycles And Methods For Their Production
  • A Cytolytic Rtx-Toxin From Gallibacterium Anatis
  • Polyketides Analogues And Methods For Their Production
  • Nucleic Acid Sequencing System And Method
  • Method For Pair-Wise Sequencing A Plurality Of Double Stranded Target Polynucleotides
  • Methods And Apparatus For Measuring Analytes Using Large Scale Fet Arrays
  • Improved Methods For Beaming
  • Methods And Apparatus For Measuring Analytes Using Large Scale Fet Arrays
  • Next-Generation Sequencing Libraries
  • Channels With Cross-Sectional Thermal Gradients
  • Nucleic Acid Preparation Compositions And Methods
  • Methods For Non-Invasive Prenatal Ploidy Calling
  • Digital Counting Of Individual Molecules By Stochastic Attachment Of Diverse Labels
  • Methods And Apparatus For Measuring Analytes
  • Methods And Systems For Digitally Counting Features On Arrays
  • Method And System For Determining Cancer Status
  • Method Of Determining The Nucleotide Sequence Of Oligonucleotides And Dna Molecules
  • Means And Methods For Non-Invasive Diagnosis Of Chromosomal Aneuploidy
  • High Throughput Screening Of Populations Carrying Naturally Occurring Mutations
  • Method For The Simultaneous Amplification Of A Plurality Of Different Nucleic Acid Target Sequences
  • Chemical Sensor Array With Leakage Compensation Circuit
  • Device And Method For Producing A Replicate Or Derivative From An Array Of Molecules, And Applications Thereof
  • Clonal Pre-Amplification In Emulsion
  • Chemically Sensitive Sensor With Lightly Doped Drains
  • Lipases, Polynucleotides Encoding Them And Their Uses
  • Biological Analysis Arrangement And Approach Therefor
  • Fluorocarbon Emulsion Stabilizing Surfactants
  • Systems And Methods For Processing Fluids
  • Titanium Nitride As Sensing Layer For Microwell Structure
  • Methods And Apparatus For Measuring Analytes Using Large Scale Fet Arrays
  • Design And Synthesis Of Cleavable Fluorescent Nucleotides As Reversible Terminators For Dna Sequencing By Synthesis
  • Integrated Sensor Arrays For Biological And Chemical Analysis
  • Two-Adaptor Library For High-Throughput Sequencing On Dna Arrays
  • Manipulation Of Fluids And Reactions In Microfluidic Systems
  • Methods And Compositions For Incorporating Nucleotides
  • Luminescent 1-Hydroxy-2-Pyridinone Chelates Of Lanthanides
  • Capture Primers And Capture Sequence Linked Solid Supports For Molecular Diagnostic Tests
  • Detecting And Classifying Copy Number Variation
  • Methods For Genome Assembly And Haplotype Phasing
  • In Vitro Evolution In Microfluidic Systems
  • Fabrication Of Tunneling Junction For Nanopore Dna Sequencing
  • Chemical Detection Circuit Including A Serializer Circuit
  • System And Method For Correcting Primer Extension Errors In Nucleic Acid Sequence Data
  • Massively Parallel Continguity Mapping
  • Chemically-Sensitive Sample And Hold Sensors
  • Enrichment Of A Target Sequence
  • One-Transistor Pixel Array With Cascoded Column Circuit
  • Two-Transistor Pixel Array
  • Methods And Apparatus For Measuring Analytes Using Large Scale Fet Arrays
  • Sequence Analysis Of Complex Amplicons
  • Microfluidic Devices, Solid Supports For Reagents And Related Methods
  • Tools And Methods For Genetic Tests Using Next Generation Sequencing
  • Methods And Compositions For Base Calling Nucleic Acids
  • Devices And Methods For Target Molecule Characterization
  • Integrated Optical Biosensor Array Including Charge Injection Circuit And Quantizer Circuit
  • Single Molecule Nucleic Acid Sequence Analysis Processes And Compositions
  • Dna Sequencing Using Multiple Metal Layer Structure With Different Organic Coatings Forming Different Transient Bondings To Dna
  • Assembly Of High Fidelity Polynucleotides
  • Methods Of Storing Information Using Nucleic Acids
  • Methods And Apparatus For Measuring Analytes Using Large Scale Fet Arrays
  • Methods And Solutions For Inhibiting Undesired Cleaving Of Labels
  • Methods And Processes For Non-Invasive Assessment Of Genetic Variations
  • Selection By Compartmentalised Screening
  • Nucleic Acid Analysis Device
  • Chemically Sensitive Sensors With Sample And Hold Capacitors
  • Asymmetric Adapter Library Construction
  • Nucleic Acid Interaction Analysis
  • Clonal Pre-Amplification In Emulsion
  • Digital Analyte Analysis
  • Detecting And Classifying Copy Number Variation
  • Diagnosis Of Lymphoid Malignancies And Minimal Residual Disease Detection
  • Massively Parallel Single Cell Analysis
  • Manipulation Of Fluids, Fluid Components And Reactions In Microfluidic Systems
  • Self-Aligned Well Structures For Low-Noise Chemical Sensors
  • Array Configuration And Readout Scheme
  • Methods And Apparatuses For Chip-Based Dna Error Reduction
  • Chemically Sensitive Sensors With Sample And Hold Capacitors
  • High Throughput Screening Of Populations Carrying Naturally Occurring Mutations
  • Recombinant Dna Constructs And Methods For Controlling Gene Expression
  • Digital Counting Of Individual Molecules By Stochastic Attachment Of Diverse Labels
  • Deducing Exon Connectivity By Rna-Templated Dna Ligation/Sequencing
  • Scaffolded Nucleic Acid Polymer Particles And Methods Of Making And Using
  • High Density Sensor Array Without Wells
  • Method And System For Delta Double Sampling
  • Dna Analysis Apparatus
  • Multipurpose Analysis Using Second Harmonic Generating Nanoprobes
  • Method For Pairwise Sequencing Of Target Polynucleotides
  • Massive Parallel Method For Decoding Dna And Rna
  • In Situ Methanogenesis Modeling And Risk Analysis
  • Apparatus And Method For Making A Replication Process Cats Or A Derivative Of An Array Of Molecules And Uses Thereof
  • Method For High-Throughtput Aflp-Based Polymorphism Detection
  • Nucleic Acid Analysis Using Sequence Tokens
  • Asymmetric Adapter Library Construction
  • Nucleic Acid Analyzer, Reaction Device For Nucleic Acid Analysis And Substrate Of Reaction Device For Nucleic Acid Analysis
  • Dna Analysis Method And Dna Analyzer
  • Method For Pair-Wise Sequencing A Plurity Of Target Polynucleotides
  • Chemical Sensor With Protruded Sensor Surface
  • Hiv-1 Detection
  • System For Reducing Noise In A Chemical Sensor Array
  • Channels With Cross-Sectional Thermal Gradients
  • Methods And Systems For Detecting Sequence Variants
  • Sequence Scheduling And Sample Distribution Techniques
  • Nucleic Acid Analysis Device
  • Methods For Sequencing A Biomolecule By Detecting Relative Positions Of Hybridized Probes
  • Transiently Bonding Drag-Tags For Separation Modalities
  • Selection By Compartmentalised Screening
  • Detection Of Nucleic Acid Reactions On Bead Arrays
  • Antibody Molecules
  • Compositions And Methods For Efficient Gene Silencing In Plants
  • Active Chemically-Sensitive Sensors
  • Method For Chemical Amplification Based On Fluid Partitioning In An Immiscible Liquid
  • System For Reducing Noise In A Chemical Sensor Array
  • Method For Pairwise Sequencing Of Target Polynucleotides
  • Massively Parallel Single Cell Analysis
  • Systems And Methods For Smart Tools In Sequence Pipelines
  • Methods For Sequencing Nucleic Acids
  • Methods And Computer Program Products For Compression Of Sequencing Data
  • Massive Parallel Method For Decoding Dna And Rna
  • Measurement And Comparison Of Immune Diversity By High-Throughput Sequencing
  • Nucleic Acid Preparation Compositions And Methods
  • Methods And Systems For Point Of Use Removal Of Sacrificial Material
  • Methods And Compositions For Clonal Amplification Of Nucleic Acid
  • Nucleic Acid Sequencing By Electrochemical Detection
  • Methods And Apparatus For Measuring Analytes
  • Analysis Of Dna
  • Method For Nucleic Acid Detection Using Voltage Enhancement
  • Massive Parallel Method For Decoding Dna And Rna
  • Method For Pairwise Sequencing Of Target Polynucleotides
  • In Situ Methanogenesis Modeling And Risk Analysis
  • Asymmetric Adapter Library Construction
  • Method For Pair-Wise Sequencing A Plurity Of Target Polynucleotides
  • Nucleic Acid Analysis Using Sequence Tokens
  • Nucleic Acid Analyzer, Reaction Device For Nucleic Acid Analysis And Substrate Of Reaction Device For Nucleic Acid Analysis
  • Method For High-Throughtput Aflp-Based Polymorphism Detection
  • Apparatus And Method For Making A Replication Process Cats Or A Derivative Of An Array Of Molecules And Uses Thereof
  • Dna Analysis Method And Dna Analyzer
  • Chemically-Sensitive Field Effect Transistor Based Pixel Array With Protection Diodes
  • Biological Analysis Arrangement And Approach Therefor
  • Independently Removable Nucleic Acid Sequencing System And Method
  • Methods And Apparatus For Measuring Analytes Using Large Scale Fet Arrays
  • System For Reducing Noise In A Chemical Sensor Array
  • Methods And Apparatus For Measuring Analytes Using Large Scale Fet Arrays
  • Methods And Apparatus For Detecting Molecular Interactions Using Fet Arrays
  • Amplification And Detection Of Ribonucleic Acid
  • Rna Analytics Method
  • Low Noise Chemically-Sensitive Field Effect Transistors
  • Chemical Sensor With Protruded Sensor Surface
  • Nanopore Based Device For Cutting Long Dna Molecules Into Fragments
  • Hiv-1 Detection
  • Methods For Operating An Array Column Integrator
  • Asymmetric Adapter Library Construction
  • Nucleic Acid Analyzer, Reaction Device For Nucleic Acid Analysis And Substrate Of Reaction Device For Nucleic Acid Analysis
  • Methods And Compositions For Large-Scale Analysis Of Nucleic Acids Using Dna Deletions
  • Methods And Apparatus To Sequence A Nucleic Acid
  • Methods For Analyzing Nucleic Acids
  • Nucleic Acid Analysis Device, Method For Producing Same, And Nucleic Acid Analyzer
  • Microorganism Nucleic Acid Purification From Host Samples
  • Fluorocarbon Emulsion Stabilizing Surfactants
  • Methods, Compositions, And Kits For Generating Rrna-Depleted Samples Or Isolating Rrna From Samples
  • Compositions And Processes For Improved Mass Spectrometry Analysis
  • Biological Analysis Arrangement And Approach Therefor
  • Digital Counting Of Individual Molecules By Stochastic Attachment Of Diverse Labels
  • Non-Invasive Diagnosis Of Graft Rejection In Organ Transplant Patients
  • Systems And Methods For Using Paired-End Data In Directed Acyclic Structure
  • Methods For Non-Invasive Prenatal Ploidy Calling
  • 3′-Oh Unblocked, Fast Photocleavable Terminating Nucleotides And Methods For Nucleic Acid Sequencing
  • Fluidic Devices, Systems, And Methods For Encapsulating And Partitioning Reagents, And Applications Of Same
  • Synthesis Of Cleavable Fluorescent Nucleotides As Reversible Terminators For Dna Sequencing By Synthesis
  • Genotyping By Next-Generation Sequencing
  • System And Method For Genotyping Using Informed Error Profiles
  • Compositions And Methods For Identifying Nucleic Acid Molecules
  • Methods Of Improving Milk Production And Compositional Characteristics
  • Temporal Regulation Of Gene Expression By Micrornas
  • Apparatus For Measuring Analytes Including Chemical Sensor Array
  • Full Cold-Pcr Enrichment With Reference Blocking Sequence
  • Methods And Compositions For Base Calling Nucleic Acids
  • Ion-Sensing Charge-Accumulation Circuits And Methods
  • Nanogap Device With Capped Nanowire Structures
  • 3′-Oh Unblocked, Fast Photocleavable Terminating Nucleotides And Methods For Nucleic Acid Sequencing
  • Massive Parallel Method For Decoding Dna And Rna
  • Biomarkers For Post-Traumatic Stress States
  • Alternative Nucleotide Flows In Sequencing-By-Synthesis Methods
  • Nucleic Acid Preparation Compositions And Methods
  • Ccd-Based Multi-Transistor Active Pixel Sensor Array
  • Microfluidic Devices, Solid Supports For Reagents And Related Methods
  • Dna Sequence Using Multiple Metal Layer Structure With Different Organic Coatings Forming Different Transient Bondings To Dna
  • Integrated Sample Preparation Systems And Stabilized Enzyme Mixtures
  • Assembly Of High Fidelity Polynucleotides
  • Assay Methods Using Nicking Endonucleases
  • Dna Sequencing With Non-Fluorescent Nucleotide Reversible Terminators And Cleavable Label Modified Nucleotide Terminators
  • Four Driving Dna - Sequencing By Means Of Synthesis With The Use Of Detachable, Reversible, Fluorescent Nucleotide Terminators
  • Two-Transistor Pixel Array
  • Dna Analysis Method And Dna Analyzer
  • Synthesis Of Cleavable Fluorescent Nucleotides As Reversible Terminators For Dna Sequencing By Synthesis
  • Active Chemically-Sensitive Sensors With In-Sensor Current Sources
  • Method For The Simultaneous Amplification Of A Plurality Of Different Nucleic Acid Target Sequences
  • Methods And Apparatus For Measuring Analytes
  • Methods And Apparatus For Measuring Analytes Using Large Scale Fet Arrays
  • Digital Analyte Analysis
  • Methods And Systems For Storing Sequence Read Data
  • Methods And Compositions For Incorporating Nucleotides
  • Manipulation Of Fluids And Reactions In Microfluidic Systems
  • Processes And Kits For Identifying Aneuploidy
  • Methods For Selecting And Amplifying Polynucleotides
  • Manipulation Of Fluids And Reactions In Microfluidic Systems
  • Ion-Sensing Charge-Accumulation Circuits And Methods
  • Chilled Reagent Container And Nucleic Acid Analyzer
  • Helmlnthosporlum Turclcum - Reslstente Plant
  • Indexing A Reference Sequence For Oligomer Sequence Mapping
  • Bead Emulsion Nucleic Acid Amplification
  • System For Reducing Noise In A Chemical Sensor Array
  • Chemical Sensor Array Having Multiple Sensors Per Well
  • Array-Based Translocation And Rearrangement Assays
  • Digital Analyte Analysis
  • Methods And Apparatus For Measuring Analytes Using Large Scale Fet Arrays
  • Massively Parallel Single Cell Analysis
  • Single-Particle Analysis Of Particle Populations
  • Nucleic Acid Preparation Compositions And Methods
  • Four-Color Dna Sequencing By Synthesis Using Cleavable Fluorescent Nucleotide Reversible Terminators
  • Resolving Genome Fractions Using Polymorphism Counts
  • Phased Small Rnas
  • Methods For Sequencing A Polynucleotide Template
  • Chemical Sensor With Conductive Cup-Shaped Sensor Surface
  • Nucleic Acid Sequencing System And Method Using A Subset Of Sites Of A Substrate
  • Method For The Synthesis Of A Bifunctional Complex
  • One-Transistor Pixel Array With Cascoded Column Circuit
  • Methods And Apparatus For Measuring Analytes Using Large Scale Fet Arrays
  • Methods And Devices For High Fidelity Polynucleotide Synthesis
  • Data Processing System And Methods
  • Analysis Using Microfluidic Partitioning Devices
  • Diamond Electrode Nanogap Transducers
  • Use Of Longitudinally Displaced Nanoscale Electrodes For Voltage Sensing Of Biomolecules And Other Analytes In Fluidic Channels
  • Chemically Sensitive Sensors With Feedback Circuits
  • Microfluidic Devices And Methods For Gene Synthesis
  • Method And Apparatus For Identifying Defects In A Chemical Sensor Array
  • Chemfet Sensor Including Floating Gate
  • Compositions And Methods For Detecting Human Pegivirus 2 (Hpgv-2)
  • Sequence Scheduling And Sample Distribution Techniques
  • Spatially Addressable Molecular Barcoding
  • Methods, Apparatuses, And Systems For Analyzing Microorganism Strains From Complex Heterogeneous Communities, Predicting And Identifying Functional Relationships And Interactions Thereof, And Selecting And Synthesizing Microbial Ensembles Based Thereon
  • Apparatus For Chemical Amplification Based On Fluid Partitioning In An Immiscible Liquid
  • Fk506 And Fk520 Analogues And Their Pharmaceutical Uses
  • Methods And Systems For Medical Sequencing Analysis
  • Stabilization Of Ozone-Labile Fluorescent Dyes By Thiourea
  • Methods And Apparatus For Detecting Molecular Interactions Using Fet Arrays
  • Methods And Compositions For Inhibiting Undesired Cleaving Of Labels
  • Screening Methods And Uses Thereof
  • Methods And Apparatus For Measuring Analytes Using Large Scale Fet Arrays
  • Methods, Apparatuses, And Systems For Analyzing Microorganism Strains From Complex Heterogeneous Communities, Predicting And Identifying Functional Relationships And Interactions Thereof, And Selecting And Synthesizing Microbial Ensembles Based Thereon
  • Method Of Determining The Nucleotide Sequence Of Oligonucleotides And Dna Molecules
  • Mapping Of Genomic Interactions
  • Methods For Genome Assembly And Haplotype Phasing
  • Methods For Accurate Sequence Data And Modified Base Position Determination
  • 3′-Oh Unblocked, Fast Photocleavable Terminating Nucleotides And Methods For Nucleic Acid Sequencing
  • Nucleic Acid Ligation Systems And Methods
  • Multiplex Methylation-Specific Amplification Systems And Methods
  • Phase-Protecting Reagent Flow Orderings For Use In Sequencing-By-Synthesis
  • Chemical Sensor With Sidewall Sensor Surface
  • Sandwich Assays In Droplets
  • System And Method For Cleaning Noisy Genetic Data And Determining Chromosome Copy Number
  • System And Method For Cleaning Noisy Genetic Data And Determining Chromosome Copy Number
  • Amplification And Analysis Of Selected Targets On Solid Supports
  • Digital Analyte Analysis
  • Use Of Galerina Marginata Genes And Proteins For Peptide Production
  • Selection Of Compartmentalized Screening Method
  • Massive Parallel Method For Decoding Dna And Rna
  • Compositions And Methods For Identification Of A Duplicate Sequencing Read
  • Haplotying Of Hla Loci With Ultra-Deep Shotgun Sequencing
  • Variant Database
  • Method Of Determining The Nucleotide Sequence Of Oligonucleotides And Dna Molecules
  • Antibody Molecules
  • Methods For Operating Chemically-Sensitive Sample And Hold Sensors
  • Devices And Methods For Determining The Length Of Biopolymers And Distances Between Probes Bound Thereto
  • Spatially Addressable Molecular Barcoding
  • Highly Selective Coated-Electrode Nanogap Transducers For The Detection Of Redox Molecules
  • Microfluidic Devices And Methods Of Use In The Formation And Control Of Nanoreactors
  • Methods And Compositions For The Extraction And Amplification Of Nucleic Acid From A Sample
  • Methods And Compositions For Universal Size-Specific Pcr
  • Ultrasensitive Biosensors
  • Quantification Of A Minority Nucleic Acid Species
  • Array Column Integrator
  • Systems And Methods For Genotyping With Graph Reference
  • Systems And Methods For Detection Of Genomic Copy Number Changes
  • Haploidome Determination By Digitized Transposons
  • Nanopore Based Sequencer
  • Methods And Apparatus For High Speed Operation Of A Chemically-Sensitive Sensor Array
  • Chemically-Sensitive Array With Active And Reference Sensors
  • Asymmetric Adapter Library Construction
  • Method For Sequencing A Polynucleotide Template
  • Systems And Methods For Analyzing Sequence Data
  • Methods And Computer Program Products For Compression Of Sequencing Data
  • Methods And Systems For Detecting Sequence Variants
  • Method For Sequencing A Polynucleotide Template
  • Method Of Amplifying Target Nucleic Acid With Reduced Amplification Bias And Method For Determining Relative Amount Of Target Nucleic Acid In Sample
  • Chemically-Sensitive Sensor Array Calibration Circuitry
  • Methods For Manufacturing Low Noise Chemically-Sensitive Field Effect Transistors
  • Active Chemically-Sensitive Sensors With In-Sensor Current Sources
  • Capacitive Charge Pump
  • Sample Multiplexing
  • Fluidics System For Sequential Delivery Of Reagents
  • Column Adc
  • Manipulating Droplet Size
  • Active Chemically-Sensitive Sensors With Correlated Double Sampling
  • Methods And Apparatus For Detecting Molecular Interactions Using Fet Arrays
  • Methods And Apparatus For Detecting Molecular Interactions Using Fet Arrays
  • Methods And Apparatus For Detecting Molecular Interactions Using Fet Arrays
  • Sequencing By Structure Assembly
  • Methods And Apparatus For Measuring Analytes Using Large Scale Fet Arrays
  • Methods And Apparatus For Measuring Analytes Using Large Scale Fet Arrays
  • Compositions And Methods For Representational Selection Of Nucleic Acids From Complex Mixtures Using Hybridization
  • Amplification Of A Sequence From A Ribonucleic Acid
  • Amplification Primers And Methods
  • Methods And Apparatus For Detecting Molecular Interactions Using Fet Arrays
  • Systems And Methods For Isolating Nucleic Acids
  • Methods For Preparative In Vitro Cloning
  • Aptamers And Uses Thereof
  • Compositions And Methods For Aptamer Screening
  • Methods And Apparatus For Measuring Analytes Using Large Scale Fet Arrays
  • Method For Pairwise Sequencing Of Target Polynucleotides
  • Anticaries Compositions And Probiotics/Prebiotics
  • Methods And Compositions For Incorporating Nucleotides
  • Active-Electrode Integrated Biosensor Array And Methods For Use Thereof
  • Quantitating High Titer Samples By Digital Pcr
  • Functionalization Of And Use Of Functionalized Second Harmonic Generating Nanoprobes
  • Nucleic Acid Analysis Reaction Cell And Nucleic Acid Analyzer
  • Dna Sequence With Non-Fluorescent Nucleotide Reversible Terminators And Cleavable Label Modified Nucleotide Terminators
  • Efficient Arrays Of Amplified Polynucleotides
  • Selection Of A Nutritional Composition Capable Of Promoting Health
  • Independently Removable Nucleic Acid Sequencing System And Method
  • Chemical Amplification Based On Fluid Partitioning In An Immiscible Liquid
  • Synthesis Of Sequence-Verified Nucleic Acids
  • Methods And Apparatus For Measuring Analytes
  • Compartmentalised Combinatorial Chemistry By Microfluidic Control
  • Use Of Galerina Marginata Genes And Proteins For Peptide Production
  • Screening For Structural Variants
  • Methods And Systems For Processing Polynucleotides
  • Functionally Switchable Self-Assembled Coating Compound For Controlling Translocation Of Molecule Through Nanopores
  • High Throughput Detection Of Molecular Markers Based On Aflp And High Through-Put Sequencing
  • Functionally Switchable Self-Assembled Coating Compound For Controlling Translocation Of Molecule Through Nanopores
  • Biomarker Associated With Irritable Bowel Syndrome And Crohn's Disease
  • Apparatus And Methods For Analysis Of Biomolecules Using High Frequency Alternating Current Excitation
  • Methods Of Sequencing With Linked Fragments
  • Reducing Sequence Read Count Error In Assessment Of Complex Genetic Variations
  • Methods For Manufacturing Well Structures For Low-Noise Chemical Sensors
  • Methods And Apparatus For Sorting Data
  • Method For Retaining Even Coverage Of Short Insert Libraries
  • Methods For Nucleic Acid Mapping And Identification Of Fine-Structural-Variations In Nucleic Acids
  • Active-Electrode Integrated Biosensor Array And Methods For Use Thereof
  • Compositions And Processes For Improved Mass Spectrometry Analysis
  • Methods And Apparatus For Measuring Analytes Using Large Scale Fet Arrays
  • Methods And Apparatus For Measuring Analytes Using Large Scale Fet Arrays
  • Method For Introducing A Sample Specific Dna Tag Into A Plurality Of Dna Fragments From A Plurality Of Samples
  • Manufacturable Sub-3 Nanometer Palladium Gap Devices For Fixed Electrode Tunneling Recognition
  • Methods And Compositions For Inhibiting Undesired Cleaving Of Labels
  • Methods And Systems For Point Of Use Removal Of Sacrificial Material
  • Spatial Sequencing Of Nucleic Acids Using Dna Origami Probes
  • Array Column Integrator
  • Methods And Compositions For Nitrogen Removal Using Feammox Microorganisms
  • Digital Counting Of Individual Molecules By Stochastic Attachment Of Diverse Labels
  • 3′-Oh Unblocked, Fast Photocleavable Terminating Nucleotides And Methods For Nucleic Acid Sequencing
  • System And Method Of Detecting Rnas Altered By Cancer In Peripheral Blood
  • Method For Chemical Amplification Based On Fluid Partitioning In An Immiscible Liquid
  • Compartmentalised Combinatorial Chemistry By Microfluidic Control
  • Methods And Apparatus For Measuring Analytes Using Large Scale Fet Arrays
  • Oligomer Sequences Mapping
  • Manipulation Of Fluids, Fluid Components And Reactions In Microfluidic Systems
  • Selective Genome Amplification
  • Compositions And Methods For Determining Whether A Subject Would Benefit From Co-Receptor Inhibitor Therapy
  • Methods And Compositions For Analyzing Nucleic Acid
  • Piscine Reovirus Immunogenic Compositions
  • Methods And Apparatus For Measuring Analytes Using Large Scale Fet Arrays
  • High Throughput Screening Of Populations Carrying Naturally Occuring Mutations
  • Macrocyclic Hopo Chelators
  • Mapping Of Genomic Interactions
  • Second Harmonic Imaging Nanoprobes And Techniques For Use Thereof
  • Nucleic Acid Sequencing
  • Nanogap Device With Capped Nanowire Structures
  • Compartmentalized Screening By Microfluidic Control
  • Methods And Processes For Non-Invasive Assessment Of Genetic Variations
  • Systems And Methods For Isolating Nucleic Acids From Cellular Samples
  • Time-Warped Background Signal For Sequencing-By-Synthesis Operations
  • Methods For Genome Assembly And Haplotype Phasing
  • Functionalization Of And Use Of Functionalized Second Harmonic Generating Nanoprobes
  • System And Method To Correct Out Of Phase Errors In Dna Sequencing Data By Use Of A Recursive Algorithm
  • Methods For Accurate Sequence Data And Modified Base Position Determination
  • Systems And Methods For Run-Time Sequencing Run Quality Monitoring
  • Methods For Producing High-Fidelity Autologous Idiotype Vaccines
  • Method For Diagnosing Allergic Reactions
  • Plant Micrornas And Methods Of Use Thereof
  • Dna Sequencing And Amplification Systems Using Nanoscale Field Effect Sensor Arrays
  • Method For Sequencing A Polynucleotide Template
  • High Throughput Genome Sequencing On Dna Arrays
  • Four-Color Dna Sequencing By Synthesis Using Cleavable Fluorescent Nucleotide Reversible Terminators
  • Method For Pairwise Sequencing Of Target Polynucleotides
  • Nucleic Acid Analysis Using Sequence Tokens
  • Quantification Of A Minority Nucleic Acid Species
  • Generation Of Ligation-Ready Dna Amplicons
  • Vitro Evolution In Microfluidic Systems
  • Microfluidic Devices And Methods Of Use In The Formation And Control Of Nanoreactors
  • Mapping Of Genomic Interactions
  • High Throughput Screening Of Populations Carrying Naturally Occurring Mutations
  • Macromolecule Delivery To Nanowells
  • Methods And Systems For Nucleic Acid Amplification
  • Generation Of Ligation-Ready Dna Amplicons
  • Vitro Evolution In Microfluidic Systems
  • Active Chemically-Sensitive Sensors With Source Follower Amplifier
  • In Vitro Evolution In Microfluidic Systems
  • Microfluidic Devices And Methods Of Use In The Formation And Control Of Nanoreactors
  • Enzyme Composition For Dna End Repair, Adenylation, Phosphorylation
  • Methods For Sorting Nucleic Acids And Multiplexed Preparative In Vitro Cloning
  • High Density Dna Array
  • Array-Based Translocation And Rearrangement Assays
  • Methods And Compositions For Tagging And Analyzing Samples
  • Methods And Systems For Identifying Contamination In Samples
  • Diagnosing Fetal Chromosomal Aneuploidy Using Genomic Sequencing
  • Nanopore Based Device For Cutting Long Dna Molecules Into Fragments
  • Manipulation Of Microfluidic Droplets
  • Nucleic Acid Analysis Using Sequence Tokens
  • Quantification Of A Minority Nucleic Acid Species
  • Two-Transistor Pixel Array
  • Preparation Enhancements And Methods Of Use For Maldi Mass Spectrometry
  • Quantification Of A Minority Nucleic Acid Species
  • One-Transistor Pixel Array
  • Microfluidic Devices And Methods For Gene Synthesis
  • Systems And Methods For Use Of Known Alleles In Read Mapping
  • Chemically-Sensitive Sample And Hold Sensors
  • Methods For Operating Chemically Sensitive Sensors With Sample And Hold Capacitors
  • Massively Parallel Single Cell Analysis
  • Use Of Galerina Marginata Genes And Proteins For Peptide Production
  • Methods Of Selecting Biologic-Producing Cell Lines By Next Generation Sequencing
  • Digital Analyte Analysis
  • Compositions And Methods For Molecular Labeling
  • Device And Method For Producing A Replicate Or Derivative From An Array Of Molecules, And Applications Thereof
  • Methods And Compositions For Incorporating Nucleotides
  • Method For Analyzing Nucleic Acid Derived From Single Cell
  • One-Transistor Pixel Array
  • Electron Beam Sculpting Of Tunneling Junction For Nanopore Dna Sequencing
  • Methods And Apparatus For Measuring Analytes Using Large Scale Fet Arrays
  • Models For Analyzing Data From Sequencing-By-Synthesis Operations
  • Programmable Oligonucleotide Synthesis
  • Methods And Apparatus For Measuring Analytes Using Large Scale Fet Arrays
  • Methods And Systems For Aligning Sequences
  • Assay Methods Using Nicking Endonucleases
  • Control Nucleic Acid Sequences For Use In Sequencing-By-Synthesis And Methods For Designing The Same
  • Method Of Synthesis And Testing Of Combinatorial Libraries Using Microcapsules
  • Digital Counting Of Individual Molecules By Stochastic Attachment Of Diverse Labels
  • Device For Nucleic Acid Analysis And Nucleic Acid Analyzer
  • Method For Separating Homoduplexed And Heteroduplexed Nucleic Acids
  • Methods And Apparatus For Measuring Analytes Using Large Scale Fet Arrays
  • Massive Parallel Method For Decoding Dna And Rna
  • Method For Generating Clonotype Profiles Using Sequence Tags
  • Massively Parallel Single Cell Analysis
  • Methods To Diagnose And Immunize Against The Virus Causing Human Merkel Cell Carcinoma
  • Biological Analysis Arrangement And Approach Therefor
  • System And Method For Producing Functionally Distinct Nucleic Acid Library Ends Through Use Of Deoxyinosine
  • Method For Identifying The Sequence Of One Or More Variant Nucleotides In A Nucleic Acid Molecule
  • Nucleic Acid Analysis Using Sequence Tokens
  • Manufacturable Sub-3 Nanometer Palladium Gap Devices For Fixed Electrode Tunneling Recognition
  • Massive Parallel Method For Decoding Dna And Rna
  • Method For Determining Fetal Chromosomal Abnormality
  • Methods And Apparatus For Measuring Analytes
  • Solid Support For High-Throughput Nucleic Acid Analysis
  • Data Processing System And Methods
  • Methods For Accurate Sequence Data And Modified Base Position Determination
  • Paired End Bead Amplification And High Throughput Sequencing
  • Chemical Sensor With Protruded Sensor Surface
  • Nanogap Transducers With Selective Surface Immobilization Sites
  • Methods And Solutions For Inhibiting Undesired Cleaving Of Labels
  • Oligomer Sequences Mapping
  • Massive Parallel Method For Decoding Dna And Rna
  • Methods For Forming Mixed Droplets
  • Piscine Reovirus Diagnostic Compositions
  • Electron Beam Sculpting Of Tunneling Junction For Nanopore Dna Sequencing
  • Detection And Quantification Of Biomolecules Using Mass Spectrometry
  • Chemical Sensor With Sidewall Spacer Sensor Surface
  • Active Chemically-Sensitive Sensors With Reset Switch
  • Four-Color Dna Sequencing By Synthesis Using Cleavable Fluorescent Nucleotide Reversible Terminators
  • Method And System For Delta Double Sampling
  • Detecting And Classifying Copy Number Variation
  • High Throughput Genome Sequencing On Dna Arrays
  • Chemical Sensors With Consistent Sensor Surface Areas
  • Methods, Compounds And Systems For Detecting A Microorganism In A Sample
  • Method Of Performing One-Step, Single Cell Rt-Pcr
  • Dna Sequencing Using Multiple Metal Layer Structure With Different Organic Coatings Forming Different Transient Bondings To Dna
  • Amplification Primers And Methods
  • Method For Sequencing A Polynucleotide Template
  • Methods And Apparatus For Measuring Analytes Using Large Scale Fet Arrays
  • Composition, Method And Kit For Detecting Bacteria By Means Of Sequencing
  • Integrated Sample Preparation Systems And Stabilized Enzyme Mixtures
  • Methods For Estimating Genome-Wide Copy Number Variations
  • Massively Parallel Single Cell Analysis
  • Second Harmonic Imaging Nanoprobes And Techniques For Use Thereof
  • Variant Database
  • Analysis Methods
  • Efficient Arrays Of Amplified Polynucleotides
  • Method And System For Delta Double Sampling
  • Nucleic Acid Interaction Analysis
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nature03959

    DOI

    http://dx.doi.org/10.1038/nature03959

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1021574562

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/16056220


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Electrophoresis, Capillary", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Emulsions", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Fiber Optic Technology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genome, Bacterial", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genomics", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Microchemistry", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mycoplasma genitalium", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Polymerase Chain Reaction", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Reproducibility of Results", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sensitivity and Specificity", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sequence Analysis, DNA", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Time Factors", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Margulies", 
            "givenName": "Marcel", 
            "id": "sg:person.01044174712.17", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01044174712.17"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Egholm", 
            "givenName": "Michael", 
            "id": "sg:person.0743270554.10", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0743270554.10"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Altman", 
            "givenName": "William E.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Attiya", 
            "givenName": "Said", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bader", 
            "givenName": "Joel S.", 
            "id": "sg:person.01266011105.42", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01266011105.42"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Bemben", 
            "givenName": "Lisa A.", 
            "id": "sg:person.01206136377.63", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206136377.63"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Berka", 
            "givenName": "Jan", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Braverman", 
            "givenName": "Michael S.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chen", 
            "givenName": "Yi-Ju", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chen", 
            "givenName": "Zhoutao", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Dewell", 
            "givenName": "Scott B.", 
            "id": "sg:person.01363126147.44", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363126147.44"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Du", 
            "givenName": "Lei", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Fierro", 
            "givenName": "Joseph M.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gomes", 
            "givenName": "Xavier V.", 
            "id": "sg:person.01303150226.30", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303150226.30"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Godwin", 
            "givenName": "Brian C.", 
            "id": "sg:person.01105277310.34", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105277310.34"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "He", 
            "givenName": "Wen", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Helgesen", 
            "givenName": "Scott", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ho", 
            "givenName": "Chun He", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Irzyk", 
            "givenName": "Gerard P.", 
            "id": "sg:person.07732467560.08", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07732467560.08"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jando", 
            "givenName": "Szilveszter C.", 
            "id": "sg:person.0651240437.72", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0651240437.72"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Alenquer", 
            "givenName": "Maria L. I.", 
            "id": "sg:person.0717353637.60", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0717353637.60"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jarvie", 
            "givenName": "Thomas P.", 
            "id": "sg:person.01303204410.61", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303204410.61"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jirage", 
            "givenName": "Kshama B.", 
            "id": "sg:person.0706573670.94", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0706573670.94"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kim", 
            "givenName": "Jong-Bum", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Knight", 
            "givenName": "James R.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lanza", 
            "givenName": "Janna R.", 
            "id": "sg:person.01150674650.09", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01150674650.09"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Leamon", 
            "givenName": "John H.", 
            "id": "sg:person.0766333050.47", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0766333050.47"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lefkowitz", 
            "givenName": "Steven M.", 
            "id": "sg:person.01101417410.20", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101417410.20"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lei", 
            "givenName": "Ming", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Li", 
            "givenName": "Jing", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lohman", 
            "givenName": "Kenton L.", 
            "id": "sg:person.0716005206.35", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0716005206.35"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lu", 
            "givenName": "Hong", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Makhijani", 
            "givenName": "Vinod B.", 
            "id": "sg:person.01302625054.28", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01302625054.28"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "McDade", 
            "givenName": "Keith E.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "McKenna", 
            "givenName": "Michael P.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of California, Berkeley", 
              "id": "https://www.grid.ac/institutes/grid.47840.3f", 
              "name": [
                "University of California, Berkeley, California 94720, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Myers", 
            "givenName": "Eugene W.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nickerson", 
            "givenName": "Elizabeth", 
            "id": "sg:person.01270120337.03", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270120337.03"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nobile", 
            "givenName": "John R.", 
            "id": "sg:person.0757706452.90", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0757706452.90"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Plant", 
            "givenName": "Ramona", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Puc", 
            "givenName": "Bernard P.", 
            "id": "sg:person.016227327221.50", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016227327221.50"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ronan", 
            "givenName": "Michael T.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Roth", 
            "givenName": "George T.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sarkis", 
            "givenName": "Gary J.", 
            "id": "sg:person.012254677074.75", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012254677074.75"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Simons", 
            "givenName": "Jan Fredrik", 
            "id": "sg:person.01322004603.53", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01322004603.53"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Simpson", 
            "givenName": "John W.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Srinivasan", 
            "givenName": "Maithreyan", 
            "id": "sg:person.01055221101.48", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01055221101.48"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Tartaro", 
            "givenName": "Karrie R.", 
            "id": "sg:person.01102561450.83", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102561450.83"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Rockefeller University", 
              "id": "https://www.grid.ac/institutes/grid.134907.8", 
              "name": [
                "Laboratory of Microbiology, The Rockefeller University, New York, New York 10021, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Tomasz", 
            "givenName": "Alexander", 
            "id": "sg:person.01350744310.43", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01350744310.43"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Vogt", 
            "givenName": "Kari A.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Volkmer", 
            "givenName": "Greg A.", 
            "id": "sg:person.0664603737.37", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0664603737.37"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wang", 
            "givenName": "Shally H.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wang", 
            "givenName": "Yong", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Rothberg Institute For Childhood Diseases", 
              "id": "https://www.grid.ac/institutes/grid.417523.6", 
              "name": [
                "The Rothberg Institute for Childhood Diseases, 530 Whitfield Street, Guilford, Connecticut 06437, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Weiner", 
            "givenName": "Michael P.", 
            "id": "sg:person.0710631016.45", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0710631016.45"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yu", 
            "givenName": "Pengguang", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Begley", 
            "givenName": "Richard F.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Roche (United States)", 
              "id": "https://www.grid.ac/institutes/grid.418158.1", 
              "name": [
                "454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Rothberg", 
            "givenName": "Jonathan M.", 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1080/07391102.1989.10507773", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002321858"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0888-7543(88)90007-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012677419"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/abio.1993.1024", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014768898"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0022-5193(88)80246-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016652570"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.071052198", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023639294"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/1050-3862(91)90032-m", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023785318"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/1050-3862(91)90032-m", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023785318"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.74.12.5463", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025360556"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/abio.1996.0432", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027675298"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt0798-652", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033712419", 
              "https://doi.org/10.1038/nbt0798-652"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/elps.200305646", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035262548"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.1133470100", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045398840"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gr.8.3.175", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048253030"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1089/cmb.1995.2.275", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1059245098"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/bioinformatics/9.2.221", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1059414264"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1101/gr.11.1.3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060407391"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1109/jproc.1998.658762", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1061295809"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1061217", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062445088"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.2443975", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062537979"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.270.5235.397", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062551464"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.281.5375.363", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062561814"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082759757", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2005-09", 
        "datePublishedReg": "2005-09-01", 
        "description": "The proliferation of large-scale DNA-sequencing projects in recent years has driven a search for alternative methods to reduce time and cost. Here we describe a scalable, highly parallel sequencing system with raw throughput significantly greater than that of state-of-the-art capillary electrophoresis instruments. The apparatus uses a novel fibre-optic slide of individual wells and is able to sequence 25 million bases, at 99% or better accuracy, in one four-hour run. To achieve an approximately 100-fold increase in throughput over current Sanger sequencing technology, we have developed an emulsion method for DNA amplification and an instrument for sequencing by synthesis using a pyrosequencing protocol optimized for solid support and picolitre-scale volumes. Here we show the utility, throughput, accuracy and robustness of this system by shotgun sequencing and de novo assembly of the Mycoplasma genitalium genome with 96% coverage at 99.96% accuracy in one run of the machine.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nature03959", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.2529226", 
            "type": "MonetaryGrant"
          }, 
          {
            "id": "sg:grant.2436672", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1018957", 
            "issn": [
              "0090-0028", 
              "1476-4687"
            ], 
            "name": "Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "7057", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "437"
          }
        ], 
        "name": "Genome sequencing in microfabricated high-density picolitre reactors", 
        "pagination": "376", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "66cad864339bc29f3d2c946a2964ec68bd513b00a2ea5db730ad5eaf6f9e9457"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "16056220"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "0410462"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nature03959"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1021574562"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nature03959", 
          "https://app.dimensions.ai/details/publication/pub.1021574562"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T12:11", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000361_0000000361/records_53978_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://www.nature.com/articles/nature03959"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature03959'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature03959'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature03959'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature03959'


     

    This table displays all metadata directly associated to this object as RDF triples.

    549 TRIPLES      21 PREDICATES      62 URIs      33 LITERALS      21 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nature03959 schema:about N2d810387df8a4cff8b1c00b62c3d2bc8
    2 N3b88706c539943969f07f19eb0cce6f3
    3 N5c0d885b8076432289b9d155f33f6a7a
    4 N72d9db26667a4c2fb3ae5e4ed899adcb
    5 N7614a0a66d64409499365b1e24abb4ce
    6 N98999c8a299841ffbdacdea9c7fa13e3
    7 Na8b61da649c44f49b0ddce716a6fd178
    8 Nd7efa4bc8d0a444f81468fee8c301217
    9 Nf570e4299fc04c64a0a63730f8bb4290
    10 Nf6fd26aae4df4325b8da7bddcbecf209
    11 Nf85436ea940f436bbf5d15b454f39f9d
    12 Nfa824df2bb7a4719ada29df93dbbc41c
    13 anzsrc-for:06
    14 anzsrc-for:0604
    15 schema:author N4176b5b859984ae7bff996a578f5b98e
    16 schema:citation sg:pub.10.1038/nbt0798-652
    17 https://app.dimensions.ai/details/publication/pub.1082759757
    18 https://doi.org/10.1002/elps.200305646
    19 https://doi.org/10.1006/abio.1993.1024
    20 https://doi.org/10.1006/abio.1996.0432
    21 https://doi.org/10.1016/0888-7543(88)90007-9
    22 https://doi.org/10.1016/1050-3862(91)90032-m
    23 https://doi.org/10.1016/s0022-5193(88)80246-7
    24 https://doi.org/10.1073/pnas.071052198
    25 https://doi.org/10.1073/pnas.1133470100
    26 https://doi.org/10.1073/pnas.74.12.5463
    27 https://doi.org/10.1080/07391102.1989.10507773
    28 https://doi.org/10.1089/cmb.1995.2.275
    29 https://doi.org/10.1093/bioinformatics/9.2.221
    30 https://doi.org/10.1101/gr.11.1.3
    31 https://doi.org/10.1101/gr.8.3.175
    32 https://doi.org/10.1109/jproc.1998.658762
    33 https://doi.org/10.1126/science.1061217
    34 https://doi.org/10.1126/science.2443975
    35 https://doi.org/10.1126/science.270.5235.397
    36 https://doi.org/10.1126/science.281.5375.363
    37 schema:datePublished 2005-09
    38 schema:datePublishedReg 2005-09-01
    39 schema:description The proliferation of large-scale DNA-sequencing projects in recent years has driven a search for alternative methods to reduce time and cost. Here we describe a scalable, highly parallel sequencing system with raw throughput significantly greater than that of state-of-the-art capillary electrophoresis instruments. The apparatus uses a novel fibre-optic slide of individual wells and is able to sequence 25 million bases, at 99% or better accuracy, in one four-hour run. To achieve an approximately 100-fold increase in throughput over current Sanger sequencing technology, we have developed an emulsion method for DNA amplification and an instrument for sequencing by synthesis using a pyrosequencing protocol optimized for solid support and picolitre-scale volumes. Here we show the utility, throughput, accuracy and robustness of this system by shotgun sequencing and de novo assembly of the Mycoplasma genitalium genome with 96% coverage at 99.96% accuracy in one run of the machine.
    40 schema:genre research_article
    41 schema:inLanguage en
    42 schema:isAccessibleForFree true
    43 schema:isPartOf N1860bd51312c4eebbeccebc1bdf85997
    44 Ndf7e0ab6d232442da61812cde7528613
    45 sg:journal.1018957
    46 schema:name Genome sequencing in microfabricated high-density picolitre reactors
    47 schema:pagination 376
    48 schema:productId N10599ee416164b6ca7233ad30f8c0d69
    49 N27750d8c15e341a2aca3a7b047dae798
    50 N2fc3e6a396aa43b2999ab591a5dc8b36
    51 N3d22d3ed923f409fa0c8099ed519a84a
    52 Ne53f911290b0485ebf2519838b0410e1
    53 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021574562
    54 https://doi.org/10.1038/nature03959
    55 schema:sdDatePublished 2019-04-11T12:11
    56 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    57 schema:sdPublisher Nc9498db426394941adc16f4598db7951
    58 schema:url https://www.nature.com/articles/nature03959
    59 sgo:license sg:explorer/license/
    60 sgo:sdDataset articles
    61 rdf:type schema:ScholarlyArticle
    62 N0b6d2d546166489ea97ae4c38fa6d1e5 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    63 schema:familyName Simpson
    64 schema:givenName John W.
    65 rdf:type schema:Person
    66 N0f6774a642f14ae4999c4757561ec1b4 rdf:first sg:person.01102561450.83
    67 rdf:rest N1943927fced24d209fa6f7a3dff4d51b
    68 N10599ee416164b6ca7233ad30f8c0d69 schema:name pubmed_id
    69 schema:value 16056220
    70 rdf:type schema:PropertyValue
    71 N112c00228e75412c92285b574eb2eb40 rdf:first sg:person.0717353637.60
    72 rdf:rest N861735dc5a1f4fd4a664b9220ec1bd9a
    73 N1170ea26e95441caa3b41fbea10530b5 rdf:first N819914a2e95d4dc196b1a04f4f7dedae
    74 rdf:rest N7027c400b36d418198996fba5ae62767
    75 N152251f21d344636895a8420539b5634 rdf:first N67318890fd054956ae302d033e7693a0
    76 rdf:rest N33bd74f24b4143d3a66cdb70e24ed282
    77 N1723105b0aff4727ac3b67dbeecf7149 rdf:first sg:person.0710631016.45
    78 rdf:rest Nc9bed5c52cac4620a490fe9c0ee78f15
    79 N17aa23725e6b4f2f93c1f8fdd672ddaa rdf:first Neb643446738a4ad685ad8abfe6bbdca6
    80 rdf:rest N20b2d53facd54225ac8422117f20d40e
    81 N1860bd51312c4eebbeccebc1bdf85997 schema:volumeNumber 437
    82 rdf:type schema:PublicationVolume
    83 N1943927fced24d209fa6f7a3dff4d51b rdf:first sg:person.01350744310.43
    84 rdf:rest N9ebfdee0ea05420f9a2de9dcc7aff86b
    85 N1eb144d52c5e40d7914f0c886b7ec82f rdf:first sg:person.01266011105.42
    86 rdf:rest Nfd53f58a466845708d14ebb75f0891fb
    87 N1f4ee450b67d475784a482efbd5b7e36 rdf:first sg:person.0706573670.94
    88 rdf:rest N5ebb076209a140fc9d8ed1f95e943777
    89 N20b2d53facd54225ac8422117f20d40e rdf:first N3d0c8964565e4b868102b868058b1099
    90 rdf:rest Naa526b2ef8524b3b9f5058a48afc7bdd
    91 N27750d8c15e341a2aca3a7b047dae798 schema:name dimensions_id
    92 schema:value pub.1021574562
    93 rdf:type schema:PropertyValue
    94 N27e6111682e246d6a01c62aa661e4465 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    95 schema:familyName Fierro
    96 schema:givenName Joseph M.
    97 rdf:type schema:Person
    98 N2c38de885ed245caa303bcd49f200f29 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    99 schema:familyName Knight
    100 schema:givenName James R.
    101 rdf:type schema:Person
    102 N2d810387df8a4cff8b1c00b62c3d2bc8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    103 schema:name Reproducibility of Results
    104 rdf:type schema:DefinedTerm
    105 N2fc3e6a396aa43b2999ab591a5dc8b36 schema:name nlm_unique_id
    106 schema:value 0410462
    107 rdf:type schema:PropertyValue
    108 N33bd74f24b4143d3a66cdb70e24ed282 rdf:first Na1d9acdac3c24ef5950ddf32f6f0e957
    109 rdf:rest N1170ea26e95441caa3b41fbea10530b5
    110 N3439ff8159814364adc2d67553faf5c6 rdf:first sg:person.0651240437.72
    111 rdf:rest N112c00228e75412c92285b574eb2eb40
    112 N3844815d1b4c4cedbec59fcde0a49ff5 schema:affiliation https://www.grid.ac/institutes/grid.47840.3f
    113 schema:familyName Myers
    114 schema:givenName Eugene W.
    115 rdf:type schema:Person
    116 N3b88706c539943969f07f19eb0cce6f3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    117 schema:name Mycoplasma genitalium
    118 rdf:type schema:DefinedTerm
    119 N3d0c8964565e4b868102b868058b1099 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    120 schema:familyName Ho
    121 schema:givenName Chun He
    122 rdf:type schema:Person
    123 N3d22d3ed923f409fa0c8099ed519a84a schema:name readcube_id
    124 schema:value 66cad864339bc29f3d2c946a2964ec68bd513b00a2ea5db730ad5eaf6f9e9457
    125 rdf:type schema:PropertyValue
    126 N4176b5b859984ae7bff996a578f5b98e rdf:first sg:person.01044174712.17
    127 rdf:rest N6af17c2d1f964fac93dce8dc016fce4a
    128 N465889ea15c1469b981d7a1be110f671 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    129 schema:familyName Yu
    130 schema:givenName Pengguang
    131 rdf:type schema:Person
    132 N4766e62d7b4342588b5b127dc04e4ad6 rdf:first N6f696ece59fd4437a342f2431997395f
    133 rdf:rest N17aa23725e6b4f2f93c1f8fdd672ddaa
    134 N511c0833bcb84c3781cbb4f1bc5b65ed rdf:first Nb2042a51c5b14a419324efc380df25f3
    135 rdf:rest Nce91cc8edbe64384a05a9dca223635fb
    136 N52e9717c870e43bea70d371057fa9832 rdf:first sg:person.0757706452.90
    137 rdf:rest N72c06fd8af654c5c837cee381e1ba61a
    138 N551ed4c81a404b7e86c8e2632b402e29 rdf:first sg:person.01303150226.30
    139 rdf:rest Ne4a204a3ac03494f97718c3cfe4803d0
    140 N558beee38dac455cbb4cdd0bf976cef4 rdf:first N58d8d7e6bbf344e89dc68001e210aa0d
    141 rdf:rest Nbd3d6d4c037d49c0b88ceac2231bc445
    142 N58d8d7e6bbf344e89dc68001e210aa0d schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    143 schema:familyName Lu
    144 schema:givenName Hong
    145 rdf:type schema:Person
    146 N5ad6b195685f4464971f7c91adc204bd schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    147 schema:familyName Wang
    148 schema:givenName Shally H.
    149 rdf:type schema:Person
    150 N5b1d22e58a4b4f599c2fbb061fd97327 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    151 schema:familyName Plant
    152 schema:givenName Ramona
    153 rdf:type schema:Person
    154 N5c0d885b8076432289b9d155f33f6a7a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    155 schema:name Genomics
    156 rdf:type schema:DefinedTerm
    157 N5ebb076209a140fc9d8ed1f95e943777 rdf:first Ndd4482aa6b664301828405c506e70b9d
    158 rdf:rest N89a6729d990741a1a437fb2ab4215a14
    159 N5fc8bd2820c8425b8cdd9bfd1b07222f rdf:first Na059e7f1d70c4e909ac7bee07278d584
    160 rdf:rest N6eee3277d02c4ef78973da30fd0b22cc
    161 N60500d488e584012ba8957d66f8c3a11 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    162 schema:familyName Roth
    163 schema:givenName George T.
    164 rdf:type schema:Person
    165 N67318890fd054956ae302d033e7693a0 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    166 schema:familyName Braverman
    167 schema:givenName Michael S.
    168 rdf:type schema:Person
    169 N6af17c2d1f964fac93dce8dc016fce4a rdf:first sg:person.0743270554.10
    170 rdf:rest N511c0833bcb84c3781cbb4f1bc5b65ed
    171 N6c487b7166614ab9a336f053a62c9319 rdf:first N5ad6b195685f4464971f7c91adc204bd
    172 rdf:rest Nc7257b5ddc284e6d8f5fdd1160adfe3a
    173 N6eee3277d02c4ef78973da30fd0b22cc rdf:first Ndc74e5ad3aa245f5abac0df9ae4cf78b
    174 rdf:rest Na99e624960f244f693e48447b83e9e4c
    175 N6f696ece59fd4437a342f2431997395f schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    176 schema:familyName He
    177 schema:givenName Wen
    178 rdf:type schema:Person
    179 N7027c400b36d418198996fba5ae62767 rdf:first sg:person.01363126147.44
    180 rdf:rest Nc9c8a6a6c42f406e80a9ef6e3cf98f80
    181 N7170896e67cd407ba11200b5a585a5f8 rdf:first sg:person.01322004603.53
    182 rdf:rest N94853c5adacb4d1b860e41471c47e761
    183 N72c06fd8af654c5c837cee381e1ba61a rdf:first N5b1d22e58a4b4f599c2fbb061fd97327
    184 rdf:rest Naa46a8b77d564f4b89f76e258e85914b
    185 N72d9db26667a4c2fb3ae5e4ed899adcb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    186 schema:name Microchemistry
    187 rdf:type schema:DefinedTerm
    188 N7410373c96724d039ce0478789f0a142 rdf:first Nf0905967741340daaf8dc2d98d0bc570
    189 rdf:rest Nf54b84b79e444c268517eec669cb0342
    190 N74ec76db214b4ba7b0bfe4966262c35a rdf:first sg:person.012254677074.75
    191 rdf:rest N7170896e67cd407ba11200b5a585a5f8
    192 N7614a0a66d64409499365b1e24abb4ce schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    193 schema:name Emulsions
    194 rdf:type schema:DefinedTerm
    195 N7fb263e3adc94505a65e2359804d60e4 rdf:first sg:person.01055221101.48
    196 rdf:rest N0f6774a642f14ae4999c4757561ec1b4
    197 N819914a2e95d4dc196b1a04f4f7dedae schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    198 schema:familyName Chen
    199 schema:givenName Zhoutao
    200 rdf:type schema:Person
    201 N861735dc5a1f4fd4a664b9220ec1bd9a rdf:first sg:person.01303204410.61
    202 rdf:rest N1f4ee450b67d475784a482efbd5b7e36
    203 N89a6729d990741a1a437fb2ab4215a14 rdf:first N2c38de885ed245caa303bcd49f200f29
    204 rdf:rest N8ec1084d81a24867a3fe7d15641ad78c
    205 N8ec1084d81a24867a3fe7d15641ad78c rdf:first sg:person.01150674650.09
    206 rdf:rest Ne14107ab491a4e5c8aa02463c5691815
    207 N94853c5adacb4d1b860e41471c47e761 rdf:first N0b6d2d546166489ea97ae4c38fa6d1e5
    208 rdf:rest N7fb263e3adc94505a65e2359804d60e4
    209 N95cd268fafc4458ca3c6e11a1983b3e9 rdf:first Nfab8ac306f944217af8e38fdd69957b8
    210 rdf:rest N7410373c96724d039ce0478789f0a142
    211 N97e25724006f4125bf979be94ef65336 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    212 schema:familyName Begley
    213 schema:givenName Richard F.
    214 rdf:type schema:Person
    215 N98999c8a299841ffbdacdea9c7fa13e3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    216 schema:name Sensitivity and Specificity
    217 rdf:type schema:DefinedTerm
    218 N9ca3d8bc962d4e5d8665d154256af546 rdf:first Nbecad4d3135847509b8fb244c0eb79f7
    219 rdf:rest rdf:nil
    220 N9cb149a812f04e34accad378d9374575 rdf:first Nd7d7ab59f9304622bb8bf1be39bbd4ce
    221 rdf:rest N152251f21d344636895a8420539b5634
    222 N9ebfdee0ea05420f9a2de9dcc7aff86b rdf:first Na7888c2231ca4488a6c942c6a5e40be0
    223 rdf:rest Nf1b0f8b4c21a42c8b59fd4629f695564
    224 Na059e7f1d70c4e909ac7bee07278d584 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    225 schema:familyName McDade
    226 schema:givenName Keith E.
    227 rdf:type schema:Person
    228 Na1d9acdac3c24ef5950ddf32f6f0e957 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    229 schema:familyName Chen
    230 schema:givenName Yi-Ju
    231 rdf:type schema:Person
    232 Na7888c2231ca4488a6c942c6a5e40be0 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    233 schema:familyName Vogt
    234 schema:givenName Kari A.
    235 rdf:type schema:Person
    236 Na8b61da649c44f49b0ddce716a6fd178 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    237 schema:name Fiber Optic Technology
    238 rdf:type schema:DefinedTerm
    239 Na99e624960f244f693e48447b83e9e4c rdf:first N3844815d1b4c4cedbec59fcde0a49ff5
    240 rdf:rest Nbae78e31b44a42cdb7a1a1b2697d1293
    241 Naa46a8b77d564f4b89f76e258e85914b rdf:first sg:person.016227327221.50
    242 rdf:rest Naf918a6a1651493486c3481e93fb55d7
    243 Naa526b2ef8524b3b9f5058a48afc7bdd rdf:first sg:person.07732467560.08
    244 rdf:rest N3439ff8159814364adc2d67553faf5c6
    245 Naf918a6a1651493486c3481e93fb55d7 rdf:first Ncea5eced2d0d489daf7cf50507701b80
    246 rdf:rest Nd3b351540c7e4222b7503ca764369df0
    247 Nb2042a51c5b14a419324efc380df25f3 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    248 schema:familyName Altman
    249 schema:givenName William E.
    250 rdf:type schema:Person
    251 Nbae78e31b44a42cdb7a1a1b2697d1293 rdf:first sg:person.01270120337.03
    252 rdf:rest N52e9717c870e43bea70d371057fa9832
    253 Nbd3d6d4c037d49c0b88ceac2231bc445 rdf:first sg:person.01302625054.28
    254 rdf:rest N5fc8bd2820c8425b8cdd9bfd1b07222f
    255 Nbecad4d3135847509b8fb244c0eb79f7 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    256 schema:familyName Rothberg
    257 schema:givenName Jonathan M.
    258 rdf:type schema:Person
    259 Nc06bc54d7e5846dd91b69ed23746ce3e schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    260 schema:familyName Du
    261 schema:givenName Lei
    262 rdf:type schema:Person
    263 Nc7257b5ddc284e6d8f5fdd1160adfe3a rdf:first Nd23185d4ca9e41c584fff469ead7f7da
    264 rdf:rest N1723105b0aff4727ac3b67dbeecf7149
    265 Nc9498db426394941adc16f4598db7951 schema:name Springer Nature - SN SciGraph project
    266 rdf:type schema:Organization
    267 Nc9bed5c52cac4620a490fe9c0ee78f15 rdf:first N465889ea15c1469b981d7a1be110f671
    268 rdf:rest Nd4f330de3d644730b308766467c3f3e5
    269 Nc9c8a6a6c42f406e80a9ef6e3cf98f80 rdf:first Nc06bc54d7e5846dd91b69ed23746ce3e
    270 rdf:rest Nd9f14b74563b4b3587b195a934997c0c
    271 Nce91cc8edbe64384a05a9dca223635fb rdf:first Nddaa94908aba41a5a8004687ec68f12a
    272 rdf:rest N1eb144d52c5e40d7914f0c886b7ec82f
    273 Ncea5eced2d0d489daf7cf50507701b80 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    274 schema:familyName Ronan
    275 schema:givenName Michael T.
    276 rdf:type schema:Person
    277 Nd23185d4ca9e41c584fff469ead7f7da schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    278 schema:familyName Wang
    279 schema:givenName Yong
    280 rdf:type schema:Person
    281 Nd3b351540c7e4222b7503ca764369df0 rdf:first N60500d488e584012ba8957d66f8c3a11
    282 rdf:rest N74ec76db214b4ba7b0bfe4966262c35a
    283 Nd4f330de3d644730b308766467c3f3e5 rdf:first N97e25724006f4125bf979be94ef65336
    284 rdf:rest N9ca3d8bc962d4e5d8665d154256af546
    285 Nd7d7ab59f9304622bb8bf1be39bbd4ce schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    286 schema:familyName Berka
    287 schema:givenName Jan
    288 rdf:type schema:Person
    289 Nd7efa4bc8d0a444f81468fee8c301217 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    290 schema:name Time Factors
    291 rdf:type schema:DefinedTerm
    292 Nd9f14b74563b4b3587b195a934997c0c rdf:first N27e6111682e246d6a01c62aa661e4465
    293 rdf:rest N551ed4c81a404b7e86c8e2632b402e29
    294 Ndc74e5ad3aa245f5abac0df9ae4cf78b schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    295 schema:familyName McKenna
    296 schema:givenName Michael P.
    297 rdf:type schema:Person
    298 Ndd4482aa6b664301828405c506e70b9d schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    299 schema:familyName Kim
    300 schema:givenName Jong-Bum
    301 rdf:type schema:Person
    302 Nddaa94908aba41a5a8004687ec68f12a schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    303 schema:familyName Attiya
    304 schema:givenName Said
    305 rdf:type schema:Person
    306 Ndf7e0ab6d232442da61812cde7528613 schema:issueNumber 7057
    307 rdf:type schema:PublicationIssue
    308 Ne14107ab491a4e5c8aa02463c5691815 rdf:first sg:person.0766333050.47
    309 rdf:rest Nfc2c7026b9f8455b9f13f4cf2691e8ce
    310 Ne4a204a3ac03494f97718c3cfe4803d0 rdf:first sg:person.01105277310.34
    311 rdf:rest N4766e62d7b4342588b5b127dc04e4ad6
    312 Ne53f911290b0485ebf2519838b0410e1 schema:name doi
    313 schema:value 10.1038/nature03959
    314 rdf:type schema:PropertyValue
    315 Neb643446738a4ad685ad8abfe6bbdca6 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    316 schema:familyName Helgesen
    317 schema:givenName Scott
    318 rdf:type schema:Person
    319 Nf0905967741340daaf8dc2d98d0bc570 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    320 schema:familyName Li
    321 schema:givenName Jing
    322 rdf:type schema:Person
    323 Nf1b0f8b4c21a42c8b59fd4629f695564 rdf:first sg:person.0664603737.37
    324 rdf:rest N6c487b7166614ab9a336f053a62c9319
    325 Nf54b84b79e444c268517eec669cb0342 rdf:first sg:person.0716005206.35
    326 rdf:rest N558beee38dac455cbb4cdd0bf976cef4
    327 Nf570e4299fc04c64a0a63730f8bb4290 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    328 schema:name Electrophoresis, Capillary
    329 rdf:type schema:DefinedTerm
    330 Nf6fd26aae4df4325b8da7bddcbecf209 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    331 schema:name Polymerase Chain Reaction
    332 rdf:type schema:DefinedTerm
    333 Nf85436ea940f436bbf5d15b454f39f9d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    334 schema:name Genome, Bacterial
    335 rdf:type schema:DefinedTerm
    336 Nfa824df2bb7a4719ada29df93dbbc41c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    337 schema:name Sequence Analysis, DNA
    338 rdf:type schema:DefinedTerm
    339 Nfab8ac306f944217af8e38fdd69957b8 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    340 schema:familyName Lei
    341 schema:givenName Ming
    342 rdf:type schema:Person
    343 Nfc2c7026b9f8455b9f13f4cf2691e8ce rdf:first sg:person.01101417410.20
    344 rdf:rest N95cd268fafc4458ca3c6e11a1983b3e9
    345 Nfd53f58a466845708d14ebb75f0891fb rdf:first sg:person.01206136377.63
    346 rdf:rest N9cb149a812f04e34accad378d9374575
    347 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    348 schema:name Biological Sciences
    349 rdf:type schema:DefinedTerm
    350 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    351 schema:name Genetics
    352 rdf:type schema:DefinedTerm
    353 sg:grant.2436672 http://pending.schema.org/fundedItem sg:pub.10.1038/nature03959
    354 rdf:type schema:MonetaryGrant
    355 sg:grant.2529226 http://pending.schema.org/fundedItem sg:pub.10.1038/nature03959
    356 rdf:type schema:MonetaryGrant
    357 sg:journal.1018957 schema:issn 0090-0028
    358 1476-4687
    359 schema:name Nature
    360 rdf:type schema:Periodical
    361 sg:person.01044174712.17 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    362 schema:familyName Margulies
    363 schema:givenName Marcel
    364 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01044174712.17
    365 rdf:type schema:Person
    366 sg:person.01055221101.48 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    367 schema:familyName Srinivasan
    368 schema:givenName Maithreyan
    369 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01055221101.48
    370 rdf:type schema:Person
    371 sg:person.01101417410.20 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    372 schema:familyName Lefkowitz
    373 schema:givenName Steven M.
    374 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101417410.20
    375 rdf:type schema:Person
    376 sg:person.01102561450.83 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    377 schema:familyName Tartaro
    378 schema:givenName Karrie R.
    379 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102561450.83
    380 rdf:type schema:Person
    381 sg:person.01105277310.34 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    382 schema:familyName Godwin
    383 schema:givenName Brian C.
    384 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105277310.34
    385 rdf:type schema:Person
    386 sg:person.01150674650.09 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    387 schema:familyName Lanza
    388 schema:givenName Janna R.
    389 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01150674650.09
    390 rdf:type schema:Person
    391 sg:person.01206136377.63 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    392 schema:familyName Bemben
    393 schema:givenName Lisa A.
    394 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206136377.63
    395 rdf:type schema:Person
    396 sg:person.012254677074.75 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    397 schema:familyName Sarkis
    398 schema:givenName Gary J.
    399 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012254677074.75
    400 rdf:type schema:Person
    401 sg:person.01266011105.42 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    402 schema:familyName Bader
    403 schema:givenName Joel S.
    404 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01266011105.42
    405 rdf:type schema:Person
    406 sg:person.01270120337.03 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    407 schema:familyName Nickerson
    408 schema:givenName Elizabeth
    409 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270120337.03
    410 rdf:type schema:Person
    411 sg:person.01302625054.28 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    412 schema:familyName Makhijani
    413 schema:givenName Vinod B.
    414 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01302625054.28
    415 rdf:type schema:Person
    416 sg:person.01303150226.30 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    417 schema:familyName Gomes
    418 schema:givenName Xavier V.
    419 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303150226.30
    420 rdf:type schema:Person
    421 sg:person.01303204410.61 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    422 schema:familyName Jarvie
    423 schema:givenName Thomas P.
    424 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01303204410.61
    425 rdf:type schema:Person
    426 sg:person.01322004603.53 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    427 schema:familyName Simons
    428 schema:givenName Jan Fredrik
    429 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01322004603.53
    430 rdf:type schema:Person
    431 sg:person.01350744310.43 schema:affiliation https://www.grid.ac/institutes/grid.134907.8
    432 schema:familyName Tomasz
    433 schema:givenName Alexander
    434 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01350744310.43
    435 rdf:type schema:Person
    436 sg:person.01363126147.44 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    437 schema:familyName Dewell
    438 schema:givenName Scott B.
    439 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363126147.44
    440 rdf:type schema:Person
    441 sg:person.016227327221.50 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    442 schema:familyName Puc
    443 schema:givenName Bernard P.
    444 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016227327221.50
    445 rdf:type schema:Person
    446 sg:person.0651240437.72 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    447 schema:familyName Jando
    448 schema:givenName Szilveszter C.
    449 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0651240437.72
    450 rdf:type schema:Person
    451 sg:person.0664603737.37 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    452 schema:familyName Volkmer
    453 schema:givenName Greg A.
    454 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0664603737.37
    455 rdf:type schema:Person
    456 sg:person.0706573670.94 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    457 schema:familyName Jirage
    458 schema:givenName Kshama B.
    459 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0706573670.94
    460 rdf:type schema:Person
    461 sg:person.0710631016.45 schema:affiliation https://www.grid.ac/institutes/grid.417523.6
    462 schema:familyName Weiner
    463 schema:givenName Michael P.
    464 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0710631016.45
    465 rdf:type schema:Person
    466 sg:person.0716005206.35 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    467 schema:familyName Lohman
    468 schema:givenName Kenton L.
    469 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0716005206.35
    470 rdf:type schema:Person
    471 sg:person.0717353637.60 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    472 schema:familyName Alenquer
    473 schema:givenName Maria L. I.
    474 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0717353637.60
    475 rdf:type schema:Person
    476 sg:person.0743270554.10 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    477 schema:familyName Egholm
    478 schema:givenName Michael
    479 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0743270554.10
    480 rdf:type schema:Person
    481 sg:person.0757706452.90 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    482 schema:familyName Nobile
    483 schema:givenName John R.
    484 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0757706452.90
    485 rdf:type schema:Person
    486 sg:person.0766333050.47 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    487 schema:familyName Leamon
    488 schema:givenName John H.
    489 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0766333050.47
    490 rdf:type schema:Person
    491 sg:person.07732467560.08 schema:affiliation https://www.grid.ac/institutes/grid.418158.1
    492 schema:familyName Irzyk
    493 schema:givenName Gerard P.
    494 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07732467560.08
    495 rdf:type schema:Person
    496 sg:pub.10.1038/nbt0798-652 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033712419
    497 https://doi.org/10.1038/nbt0798-652
    498 rdf:type schema:CreativeWork
    499 https://app.dimensions.ai/details/publication/pub.1082759757 schema:CreativeWork
    500 https://doi.org/10.1002/elps.200305646 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035262548
    501 rdf:type schema:CreativeWork
    502 https://doi.org/10.1006/abio.1993.1024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014768898
    503 rdf:type schema:CreativeWork
    504 https://doi.org/10.1006/abio.1996.0432 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027675298
    505 rdf:type schema:CreativeWork
    506 https://doi.org/10.1016/0888-7543(88)90007-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012677419
    507 rdf:type schema:CreativeWork
    508 https://doi.org/10.1016/1050-3862(91)90032-m schema:sameAs https://app.dimensions.ai/details/publication/pub.1023785318
    509 rdf:type schema:CreativeWork
    510 https://doi.org/10.1016/s0022-5193(88)80246-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016652570
    511 rdf:type schema:CreativeWork
    512 https://doi.org/10.1073/pnas.071052198 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023639294
    513 rdf:type schema:CreativeWork
    514 https://doi.org/10.1073/pnas.1133470100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045398840
    515 rdf:type schema:CreativeWork
    516 https://doi.org/10.1073/pnas.74.12.5463 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025360556
    517 rdf:type schema:CreativeWork
    518 https://doi.org/10.1080/07391102.1989.10507773 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002321858
    519 rdf:type schema:CreativeWork
    520 https://doi.org/10.1089/cmb.1995.2.275 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059245098
    521 rdf:type schema:CreativeWork
    522 https://doi.org/10.1093/bioinformatics/9.2.221 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059414264
    523 rdf:type schema:CreativeWork
    524 https://doi.org/10.1101/gr.11.1.3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060407391
    525 rdf:type schema:CreativeWork
    526 https://doi.org/10.1101/gr.8.3.175 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048253030
    527 rdf:type schema:CreativeWork
    528 https://doi.org/10.1109/jproc.1998.658762 schema:sameAs https://app.dimensions.ai/details/publication/pub.1061295809
    529 rdf:type schema:CreativeWork
    530 https://doi.org/10.1126/science.1061217 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062445088
    531 rdf:type schema:CreativeWork
    532 https://doi.org/10.1126/science.2443975 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062537979
    533 rdf:type schema:CreativeWork
    534 https://doi.org/10.1126/science.270.5235.397 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062551464
    535 rdf:type schema:CreativeWork
    536 https://doi.org/10.1126/science.281.5375.363 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062561814
    537 rdf:type schema:CreativeWork
    538 https://www.grid.ac/institutes/grid.134907.8 schema:alternateName Rockefeller University
    539 schema:name Laboratory of Microbiology, The Rockefeller University, New York, New York 10021, USA
    540 rdf:type schema:Organization
    541 https://www.grid.ac/institutes/grid.417523.6 schema:alternateName Rothberg Institute For Childhood Diseases
    542 schema:name The Rothberg Institute for Childhood Diseases, 530 Whitfield Street, Guilford, Connecticut 06437, USA
    543 rdf:type schema:Organization
    544 https://www.grid.ac/institutes/grid.418158.1 schema:alternateName Roche (United States)
    545 schema:name 454 Life Sciences Corp., 20 Commercial Street, Branford, Connecticut 06405, USA
    546 rdf:type schema:Organization
    547 https://www.grid.ac/institutes/grid.47840.3f schema:alternateName University of California, Berkeley
    548 schema:name University of California, Berkeley, California 94720, USA
    549 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...