SUMO-modified PCNA recruits Srs2 to prevent recombination during S phase View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2005-07

AUTHORS

Boris Pfander, George-Lucian Moldovan, Meik Sacher, Carsten Hoege, Stefan Jentsch

ABSTRACT

Damaged DNA, if not repaired before replication, can lead to replication fork stalling and genomic instability; however, cells can switch to different damage bypass modes that permit replication across lesions. Two main bypasses are controlled by ubiquitin modification of proliferating cell nuclear antigen (PCNA), a homotrimeric DNA-encircling protein that functions as a polymerase processivity factor and regulator of replication-linked functions. Upon DNA damage, PCNA is modified at the conserved lysine residue 164 by either mono-ubiquitin or a lysine-63-linked multi-ubiquitin chain, which induce error-prone or error-free replication bypasses of the lesions. In S phase, even in the absence of exogenous DNA damage, yeast PCNA can be alternatively modified by the small ubiquitin-related modifier protein SUMO; however the consequences of this remain controversial. Here we show by genetic analysis that SUMO-modified PCNA functionally cooperates with Srs2, a helicase that blocks recombinational repair by disrupting Rad51 nucleoprotein filaments. Moreover, Srs2 displays a preference for interacting directly with the SUMO-modified form of PCNA, owing to a specific binding site in its carboxy-terminal tail. Our finding suggests a model in which SUMO-modified PCNA recruits Srs2 in S phase in order to prevent unwanted recombination events of replicating chromosomes. More... »

PAGES

428

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nature03665

DOI

http://dx.doi.org/10.1038/nature03665

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011409053

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15931174


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromosomes, Fungal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Damage", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Helicases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Replication", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Epistasis, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutagenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phenotype", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proliferating Cell Nuclear Antigen", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Binding", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombination, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "S Phase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "SUMO-1 Protein", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Homology, Nucleic Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Substrate Specificity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ubiquitin-Conjugating Enzymes", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Max Planck Institute of Biochemistry", 
          "id": "https://www.grid.ac/institutes/grid.418615.f", 
          "name": [
            "Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pfander", 
        "givenName": "Boris", 
        "id": "sg:person.01073275017.12", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01073275017.12"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Max Planck Institute of Biochemistry", 
          "id": "https://www.grid.ac/institutes/grid.418615.f", 
          "name": [
            "Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Moldovan", 
        "givenName": "George-Lucian", 
        "id": "sg:person.01157374103.49", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01157374103.49"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Max Planck Institute of Biochemistry", 
          "id": "https://www.grid.ac/institutes/grid.418615.f", 
          "name": [
            "Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sacher", 
        "givenName": "Meik", 
        "id": "sg:person.01207523417.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207523417.41"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Max Planck Institute of Biochemistry", 
          "id": "https://www.grid.ac/institutes/grid.418615.f", 
          "name": [
            "Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hoege", 
        "givenName": "Carsten", 
        "id": "sg:person.0713144071.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0713144071.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Max Planck Institute of Biochemistry", 
          "id": "https://www.grid.ac/institutes/grid.418615.f", 
          "name": [
            "Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jentsch", 
        "givenName": "Stefan", 
        "id": "sg:person.01133324361.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01133324361.32"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/s0962-8924(02)02380-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003061007"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0962-8924(02)02380-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003061007"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1097-2765(04)00259-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003819257"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0921-8777(01)00086-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005347646"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature00991", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008683677", 
          "https://doi.org/10.1038/nature00991"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature00991", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008683677", 
          "https://doi.org/10.1038/nature00991"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.24.12.5130-5143.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011753175"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/sj.emboj.7600383", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016441336"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.mrrev.2003.10.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020997720"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.24.10.4267-4274.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022427060"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mmbr.66.4.630-670.2002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027328851"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.mrfmmm.2003.08.026", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028754580"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/30.3.732", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029697975"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01585", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030228330", 
          "https://doi.org/10.1038/nature01585"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01585", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030228330", 
          "https://doi.org/10.1038/nature01585"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35001009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035773549", 
          "https://doi.org/10.1038/35001009"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35001009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035773549", 
          "https://doi.org/10.1038/35001009"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01577", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036755053", 
          "https://doi.org/10.1038/nature01577"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01577", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036755053", 
          "https://doi.org/10.1038/nature01577"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(02)00630-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039487934"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/17.18.7211", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041747717"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.mrfmmm.2003.08.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043687907"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01965", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045850960", 
          "https://doi.org/10.1038/nature01965"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01965", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045850960", 
          "https://doi.org/10.1038/nature01965"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(01)00491-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047459960"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.147.5.981", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048852700"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/meth.1999.0901", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049769223"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/meth.1999.0901", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049769223"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/29.17.3487", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052193380"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2741/a469", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070917252"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2741/tsurimoto", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070918566"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075603687", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078433341", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079522422", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079980941", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082799074", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2005-07", 
    "datePublishedReg": "2005-07-01", 
    "description": "Damaged DNA, if not repaired before replication, can lead to replication fork stalling and genomic instability; however, cells can switch to different damage bypass modes that permit replication across lesions. Two main bypasses are controlled by ubiquitin modification of proliferating cell nuclear antigen (PCNA), a homotrimeric DNA-encircling protein that functions as a polymerase processivity factor and regulator of replication-linked functions. Upon DNA damage, PCNA is modified at the conserved lysine residue 164 by either mono-ubiquitin or a lysine-63-linked multi-ubiquitin chain, which induce error-prone or error-free replication bypasses of the lesions. In S phase, even in the absence of exogenous DNA damage, yeast PCNA can be alternatively modified by the small ubiquitin-related modifier protein SUMO; however the consequences of this remain controversial. Here we show by genetic analysis that SUMO-modified PCNA functionally cooperates with Srs2, a helicase that blocks recombinational repair by disrupting Rad51 nucleoprotein filaments. Moreover, Srs2 displays a preference for interacting directly with the SUMO-modified form of PCNA, owing to a specific binding site in its carboxy-terminal tail. Our finding suggests a model in which SUMO-modified PCNA recruits Srs2 in S phase in order to prevent unwanted recombination events of replicating chromosomes.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nature03665", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7049", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "436"
      }
    ], 
    "name": "SUMO-modified PCNA recruits Srs2 to prevent recombination during S phase", 
    "pagination": "428", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "365b5ef3a3f0a145b86debea92305699a8faab6f516cf5733005aeb29de1f0b5"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "15931174"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nature03665"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1011409053"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nature03665", 
      "https://app.dimensions.ai/details/publication/pub.1011409053"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:59", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000365_0000000365/records_71692_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/nature03665"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature03665'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature03665'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature03665'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature03665'


 

This table displays all metadata directly associated to this object as RDF triples.

256 TRIPLES      21 PREDICATES      76 URIs      39 LITERALS      27 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nature03665 schema:about N0619e79fbaef467aa26ef104d6c44090
2 N0e76002be216488abba6e4b265d25597
3 N23c709a6591b40c095c345abf7fe1420
4 N37bd8b2bc5f74b068afc85a507066985
5 N3ab99ebd9fb5415497a01587f662059d
6 N6067528de3694c6ebada613ce40dbf1d
7 N6cd9fb64302542cca72af1b38cbaa029
8 N99fc085e73d64d0c82717f75ca6de753
9 N9a1a22bcfcb74779b5dc9f3042731876
10 Na279b4d1160f46aabe7ab7b46a42bd2c
11 Naf5f5254f10143ec88a675fef1ff386f
12 Nb47c5d03b5034797970897a865ed46f3
13 Nb6843ebe61f74104892e904880a46f91
14 Nc24728094e5f4dffa91b75029e409cd3
15 Ncf29dbdbf9ce40769e7f16e09b759d0b
16 Ne0ad20c09968480181a4fa0857fe648f
17 Ne8b3a6543dc141ae9c64630b90fa565f
18 Nf7f6811043c14406b10ddd9e5767211a
19 anzsrc-for:06
20 anzsrc-for:0601
21 schema:author Ne44f05f2482f4e0f8bffdb56d81c2335
22 schema:citation sg:pub.10.1038/35001009
23 sg:pub.10.1038/nature00991
24 sg:pub.10.1038/nature01577
25 sg:pub.10.1038/nature01585
26 sg:pub.10.1038/nature01965
27 https://app.dimensions.ai/details/publication/pub.1075603687
28 https://app.dimensions.ai/details/publication/pub.1078433341
29 https://app.dimensions.ai/details/publication/pub.1079522422
30 https://app.dimensions.ai/details/publication/pub.1079980941
31 https://app.dimensions.ai/details/publication/pub.1082799074
32 https://doi.org/10.1006/meth.1999.0901
33 https://doi.org/10.1016/j.mrfmmm.2003.08.014
34 https://doi.org/10.1016/j.mrfmmm.2003.08.026
35 https://doi.org/10.1016/j.mrrev.2003.10.001
36 https://doi.org/10.1016/s0092-8674(01)00491-3
37 https://doi.org/10.1016/s0092-8674(02)00630-x
38 https://doi.org/10.1016/s0921-8777(01)00086-6
39 https://doi.org/10.1016/s0962-8924(02)02380-2
40 https://doi.org/10.1016/s1097-2765(04)00259-x
41 https://doi.org/10.1038/sj.emboj.7600383
42 https://doi.org/10.1083/jcb.147.5.981
43 https://doi.org/10.1093/nar/17.18.7211
44 https://doi.org/10.1093/nar/29.17.3487
45 https://doi.org/10.1093/nar/30.3.732
46 https://doi.org/10.1128/mcb.24.10.4267-4274.2004
47 https://doi.org/10.1128/mcb.24.12.5130-5143.2004
48 https://doi.org/10.1128/mmbr.66.4.630-670.2002
49 https://doi.org/10.2741/a469
50 https://doi.org/10.2741/tsurimoto
51 schema:datePublished 2005-07
52 schema:datePublishedReg 2005-07-01
53 schema:description Damaged DNA, if not repaired before replication, can lead to replication fork stalling and genomic instability; however, cells can switch to different damage bypass modes that permit replication across lesions. Two main bypasses are controlled by ubiquitin modification of proliferating cell nuclear antigen (PCNA), a homotrimeric DNA-encircling protein that functions as a polymerase processivity factor and regulator of replication-linked functions. Upon DNA damage, PCNA is modified at the conserved lysine residue 164 by either mono-ubiquitin or a lysine-63-linked multi-ubiquitin chain, which induce error-prone or error-free replication bypasses of the lesions. In S phase, even in the absence of exogenous DNA damage, yeast PCNA can be alternatively modified by the small ubiquitin-related modifier protein SUMO; however the consequences of this remain controversial. Here we show by genetic analysis that SUMO-modified PCNA functionally cooperates with Srs2, a helicase that blocks recombinational repair by disrupting Rad51 nucleoprotein filaments. Moreover, Srs2 displays a preference for interacting directly with the SUMO-modified form of PCNA, owing to a specific binding site in its carboxy-terminal tail. Our finding suggests a model in which SUMO-modified PCNA recruits Srs2 in S phase in order to prevent unwanted recombination events of replicating chromosomes.
54 schema:genre research_article
55 schema:inLanguage en
56 schema:isAccessibleForFree false
57 schema:isPartOf N56167ca493d140af87a84368975783e7
58 N89c952b58bc041fa80ccd46fb006a99a
59 sg:journal.1018957
60 schema:name SUMO-modified PCNA recruits Srs2 to prevent recombination during S phase
61 schema:pagination 428
62 schema:productId N13043f7e791d42debc8dc4c42199f5c6
63 N3554a552060b4edabad76913d4e7a4de
64 N49a634ac692b4c31985a7a19f12b5454
65 N7f8fc98dbf0a4b25aee77e61586e52bb
66 N92e3fe708c1c4cfe90d737aa3bae8f38
67 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011409053
68 https://doi.org/10.1038/nature03665
69 schema:sdDatePublished 2019-04-11T12:59
70 schema:sdLicense https://scigraph.springernature.com/explorer/license/
71 schema:sdPublisher Nd49e806a67b44f0cab23f3b57dfff80e
72 schema:url https://www.nature.com/articles/nature03665
73 sgo:license sg:explorer/license/
74 sgo:sdDataset articles
75 rdf:type schema:ScholarlyArticle
76 N0619e79fbaef467aa26ef104d6c44090 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
77 schema:name Epistasis, Genetic
78 rdf:type schema:DefinedTerm
79 N0e76002be216488abba6e4b265d25597 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Saccharomyces cerevisiae Proteins
81 rdf:type schema:DefinedTerm
82 N13043f7e791d42debc8dc4c42199f5c6 schema:name doi
83 schema:value 10.1038/nature03665
84 rdf:type schema:PropertyValue
85 N23c709a6591b40c095c345abf7fe1420 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Mutagenesis
87 rdf:type schema:DefinedTerm
88 N3554a552060b4edabad76913d4e7a4de schema:name dimensions_id
89 schema:value pub.1011409053
90 rdf:type schema:PropertyValue
91 N37bd8b2bc5f74b068afc85a507066985 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Substrate Specificity
93 rdf:type schema:DefinedTerm
94 N3806e9e53d4a46cb9d66a420d5abb513 rdf:first sg:person.01207523417.41
95 rdf:rest N7178c74994944f96922b37fd8f88c236
96 N3ab99ebd9fb5415497a01587f662059d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name SUMO-1 Protein
98 rdf:type schema:DefinedTerm
99 N4949cb6ce83542e4a1563a58601682e4 rdf:first sg:person.01133324361.32
100 rdf:rest rdf:nil
101 N49a634ac692b4c31985a7a19f12b5454 schema:name pubmed_id
102 schema:value 15931174
103 rdf:type schema:PropertyValue
104 N56167ca493d140af87a84368975783e7 schema:volumeNumber 436
105 rdf:type schema:PublicationVolume
106 N6067528de3694c6ebada613ce40dbf1d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Proliferating Cell Nuclear Antigen
108 rdf:type schema:DefinedTerm
109 N6cd9fb64302542cca72af1b38cbaa029 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name DNA Damage
111 rdf:type schema:DefinedTerm
112 N7178c74994944f96922b37fd8f88c236 rdf:first sg:person.0713144071.62
113 rdf:rest N4949cb6ce83542e4a1563a58601682e4
114 N7f8fc98dbf0a4b25aee77e61586e52bb schema:name readcube_id
115 schema:value 365b5ef3a3f0a145b86debea92305699a8faab6f516cf5733005aeb29de1f0b5
116 rdf:type schema:PropertyValue
117 N89c952b58bc041fa80ccd46fb006a99a schema:issueNumber 7049
118 rdf:type schema:PublicationIssue
119 N92e3fe708c1c4cfe90d737aa3bae8f38 schema:name nlm_unique_id
120 schema:value 0410462
121 rdf:type schema:PropertyValue
122 N99fc085e73d64d0c82717f75ca6de753 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Chromosomes, Fungal
124 rdf:type schema:DefinedTerm
125 N9a1a22bcfcb74779b5dc9f3042731876 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Recombination, Genetic
127 rdf:type schema:DefinedTerm
128 Na279b4d1160f46aabe7ab7b46a42bd2c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name S Phase
130 rdf:type schema:DefinedTerm
131 Nac8857ae743041769ca35a553303f108 rdf:first sg:person.01157374103.49
132 rdf:rest N3806e9e53d4a46cb9d66a420d5abb513
133 Naf5f5254f10143ec88a675fef1ff386f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Ubiquitin-Conjugating Enzymes
135 rdf:type schema:DefinedTerm
136 Nb47c5d03b5034797970897a865ed46f3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Saccharomyces cerevisiae
138 rdf:type schema:DefinedTerm
139 Nb6843ebe61f74104892e904880a46f91 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name DNA Replication
141 rdf:type schema:DefinedTerm
142 Nc24728094e5f4dffa91b75029e409cd3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Sequence Homology, Nucleic Acid
144 rdf:type schema:DefinedTerm
145 Ncf29dbdbf9ce40769e7f16e09b759d0b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Phenotype
147 rdf:type schema:DefinedTerm
148 Nd49e806a67b44f0cab23f3b57dfff80e schema:name Springer Nature - SN SciGraph project
149 rdf:type schema:Organization
150 Ne0ad20c09968480181a4fa0857fe648f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name DNA Helicases
152 rdf:type schema:DefinedTerm
153 Ne44f05f2482f4e0f8bffdb56d81c2335 rdf:first sg:person.01073275017.12
154 rdf:rest Nac8857ae743041769ca35a553303f108
155 Ne8b3a6543dc141ae9c64630b90fa565f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
156 schema:name Protein Binding
157 rdf:type schema:DefinedTerm
158 Nf7f6811043c14406b10ddd9e5767211a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
159 schema:name Mutation
160 rdf:type schema:DefinedTerm
161 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
162 schema:name Biological Sciences
163 rdf:type schema:DefinedTerm
164 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
165 schema:name Biochemistry and Cell Biology
166 rdf:type schema:DefinedTerm
167 sg:journal.1018957 schema:issn 0090-0028
168 1476-4687
169 schema:name Nature
170 rdf:type schema:Periodical
171 sg:person.01073275017.12 schema:affiliation https://www.grid.ac/institutes/grid.418615.f
172 schema:familyName Pfander
173 schema:givenName Boris
174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01073275017.12
175 rdf:type schema:Person
176 sg:person.01133324361.32 schema:affiliation https://www.grid.ac/institutes/grid.418615.f
177 schema:familyName Jentsch
178 schema:givenName Stefan
179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01133324361.32
180 rdf:type schema:Person
181 sg:person.01157374103.49 schema:affiliation https://www.grid.ac/institutes/grid.418615.f
182 schema:familyName Moldovan
183 schema:givenName George-Lucian
184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01157374103.49
185 rdf:type schema:Person
186 sg:person.01207523417.41 schema:affiliation https://www.grid.ac/institutes/grid.418615.f
187 schema:familyName Sacher
188 schema:givenName Meik
189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207523417.41
190 rdf:type schema:Person
191 sg:person.0713144071.62 schema:affiliation https://www.grid.ac/institutes/grid.418615.f
192 schema:familyName Hoege
193 schema:givenName Carsten
194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0713144071.62
195 rdf:type schema:Person
196 sg:pub.10.1038/35001009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035773549
197 https://doi.org/10.1038/35001009
198 rdf:type schema:CreativeWork
199 sg:pub.10.1038/nature00991 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008683677
200 https://doi.org/10.1038/nature00991
201 rdf:type schema:CreativeWork
202 sg:pub.10.1038/nature01577 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036755053
203 https://doi.org/10.1038/nature01577
204 rdf:type schema:CreativeWork
205 sg:pub.10.1038/nature01585 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030228330
206 https://doi.org/10.1038/nature01585
207 rdf:type schema:CreativeWork
208 sg:pub.10.1038/nature01965 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045850960
209 https://doi.org/10.1038/nature01965
210 rdf:type schema:CreativeWork
211 https://app.dimensions.ai/details/publication/pub.1075603687 schema:CreativeWork
212 https://app.dimensions.ai/details/publication/pub.1078433341 schema:CreativeWork
213 https://app.dimensions.ai/details/publication/pub.1079522422 schema:CreativeWork
214 https://app.dimensions.ai/details/publication/pub.1079980941 schema:CreativeWork
215 https://app.dimensions.ai/details/publication/pub.1082799074 schema:CreativeWork
216 https://doi.org/10.1006/meth.1999.0901 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049769223
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1016/j.mrfmmm.2003.08.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043687907
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1016/j.mrfmmm.2003.08.026 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028754580
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1016/j.mrrev.2003.10.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020997720
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1016/s0092-8674(01)00491-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047459960
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1016/s0092-8674(02)00630-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1039487934
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1016/s0921-8777(01)00086-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005347646
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1016/s0962-8924(02)02380-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003061007
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1016/s1097-2765(04)00259-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1003819257
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1038/sj.emboj.7600383 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016441336
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1083/jcb.147.5.981 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048852700
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1093/nar/17.18.7211 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041747717
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1093/nar/29.17.3487 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052193380
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1093/nar/30.3.732 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029697975
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1128/mcb.24.10.4267-4274.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022427060
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1128/mcb.24.12.5130-5143.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011753175
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1128/mmbr.66.4.630-670.2002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027328851
249 rdf:type schema:CreativeWork
250 https://doi.org/10.2741/a469 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070917252
251 rdf:type schema:CreativeWork
252 https://doi.org/10.2741/tsurimoto schema:sameAs https://app.dimensions.ai/details/publication/pub.1070918566
253 rdf:type schema:CreativeWork
254 https://www.grid.ac/institutes/grid.418615.f schema:alternateName Max Planck Institute of Biochemistry
255 schema:name Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
256 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...