Gene transfer to plants by diverse species of bacteria View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2005-02

AUTHORS

Wim Broothaerts, Heidi J. Mitchell, Brian Weir, Sarah Kaines, Leon M. A. Smith, Wei Yang, Jorge E. Mayer, Carolina Roa-Rodríguez, Richard A. Jefferson

ABSTRACT

Agrobacterium is widely considered to be the only bacterial genus capable of transferring genes to plants. When suitably modified, Agrobacterium has become the most effective vector for gene transfer in plant biotechnology. However, the complexity of the patent landscape has created both real and perceived obstacles to the effective use of this technology for agricultural improvements by many public and private organizations worldwide. Here we show that several species of bacteria outside the Agrobacterium genus can be modified to mediate gene transfer to a number of diverse plants. These plant-associated symbiotic bacteria were made competent for gene transfer by acquisition of both a disarmed Ti plasmid and a suitable binary vector. This alternative to Agrobacterium-mediated technology for crop improvement, in addition to affording a versatile 'open source' platform for plant biotechnology, may lead to new uses of natural bacteria-plant interactions to achieve plant transformation. More... »

PAGES

629

Journal

TITLE

Nature

ISSUE

7026

VOLUME

433

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nature03309

    DOI

    http://dx.doi.org/10.1038/nature03309

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1027789323

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/15703747


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