Gene transfer to plants by diverse species of bacteria View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2005-02

AUTHORS

Wim Broothaerts, Heidi J. Mitchell, Brian Weir, Sarah Kaines, Leon M. A. Smith, Wei Yang, Jorge E. Mayer, Carolina Roa-Rodríguez, Richard A. Jefferson

ABSTRACT

Agrobacterium is widely considered to be the only bacterial genus capable of transferring genes to plants. When suitably modified, Agrobacterium has become the most effective vector for gene transfer in plant biotechnology. However, the complexity of the patent landscape has created both real and perceived obstacles to the effective use of this technology for agricultural improvements by many public and private organizations worldwide. Here we show that several species of bacteria outside the Agrobacterium genus can be modified to mediate gene transfer to a number of diverse plants. These plant-associated symbiotic bacteria were made competent for gene transfer by acquisition of both a disarmed Ti plasmid and a suitable binary vector. This alternative to Agrobacterium-mediated technology for crop improvement, in addition to affording a versatile 'open source' platform for plant biotechnology, may lead to new uses of natural bacteria-plant interactions to achieve plant transformation. More... »

PAGES

629

Journal

TITLE

Nature

ISSUE

7026

VOLUME

433

Author Affiliations

Related Patents

  • Method And Apparatus For Substantially Isolating Plant Tissues
  • Use Of Non-Agrobacterium Bacterial Species For Plant Transformation
  • Down Regulation Of Auxin Response Factor Nbtf7 To Increase Nicotine In A Plant
  • Use Of Multiple Transformation Enhancer Sequences To Improve Plant Transformation Efficiency
  • Increasing Levels Of Nicotinic Alkaloids In Plants
  • Nucleic Acid Sequences Encoding Zinc Finger Proteins
  • Use Of Multiple Transformation Enhancer Sequences To Improve Plant Transformation Efficiency
  • Nucleic Acid Sequences Encoding Transcription Factors Regulating Alkaloid Biosynthesis And Their Use In Modifying Plant Metabolism
  • Nucleic Acid Sequences Encoding Transcription Factors Regulating Alkaloid Biosynthesis And Their Use In Modifying Plant Metabolism
  • Reducing Levels Of Nicotinic Alkaloids In Plants
  • Nucleic Acid Sequences Encoding Transcription Factors Regulating Alkaloid Biosynthesis And Their Use In Modifying Plant Metabolism
  • Nucleic Acid Sequences Encoding Transcription Factors Regulating Alkaloid Biosynthesis And Their Use In Modifying Plant Metabolism
  • Modulating Plant Oil Levels
  • Use Of Multiple Transformation Enhancer Sequences To Improve Plant Transformation Efficiency
  • Method Of Transforming Cells
  • Nucleic Acid Sequences Encoding Transcription Factors Regulating Alkaloid Biosynthesis And Their Use In Modifying Plant Metabolism
  • Ochrobactrum-Mediated Transformation Of Plants
  • Reducing Levels Of Nicotinic Alkaloids In Plants
  • Nucleic Acid Encoding N-Methylputrescine Oxidase And Uses Thereof
  • Nucleic Acid Sequences Encoding Transcription Factors Regulating Alkaloid Biosynthesis And Their Use In Modifying Plant Metabolism
  • Recombinant Carrier Molecule For Expression, Delivery And Purification Of Target Polypeptides
  • Preparation And Use Of Plant Embryo Explants For Transformation
  • Compositions And Methods For Production Of Immunoglobulins
  • Use Of Non-Agrobacterium Bacterial Species For Plant Transformation
  • Preparation And Use Of Plant Embryo Explants For Transformation
  • Methods And Compositions For Processing Biomass With Elevated Levels Of Starch
  • Nucleic Acid Sequences Encoding Transcription Factors Regulating Alkaloid Biosynthesis And Their Use In Modifying Plant Metabolism
  • Altering Lignin And Wood Density
  • Late Blight Resistance Gene From Wild Potato
  • Vectors And Methods For Improved Plant Transformation Efficiency
  • Consolidated Pretreatment And Hydrolysis Of Plant Biomass Expressing Cell Wall Degrading Enzymes
  • Method For Providing Fertile Plants Via Induction Of Bbm During Transformation
  • Nucleic Acid Sequences Encoding Transcription Factors Regulating Nicotine Alkaloid Biosynthesis And Their Use In Modifying Plant Metabolism
  • Method Of Meristem Excision And Transformation
  • Bacillus Anthracis Antigens, Vaccine Compositions, And Related Methods
  • Intein-Modified Enzymes, Their Production And Industrial Applications
  • Reducing Levels Of Nicotinic Alkaloids In Plants
  • Nucleic Acid Sequences Encoding Transcription Factors Regulating Alkaloid Biosynthesis And Their Use In Modifying Plant Metabolism
  • Regulating Alkaloids
  • Method For Providing Fertile Plants Via Induction Of Bbm During Transformation
  • Transgenic Plants Expressing Civps Or Intein Modified Proteins And Related Method
  • Recombinant Carrier Molecule For Expression, Delivery And Purification Of Target Polypeptides
  • Process Of Transfecting Plants
  • Use Of Non-Agrobacterium Bacterial Species For Plant Transformation
  • Reducing Levels Of Nicotinic Alkaloids In Plants
  • Precise Cutting
  • Compositions And Methods For Improved Protein Production
  • Intein-Modified Enzymes, Their Production And Industrial Application
  • Increasing Levels Of Nicotinic Alkaloids In Plants
  • Transgenic Plants Expressing Civps Or Intein Modified Proteins And Related Method
  • Systems And Methods For Clonal Expression In Plants
  • Preparation And Use Of Plant Embryo Explants For Transformation
  • Humanized Neuraminidase Antibody And Methods Of Use Thereof
  • Influenza Hemagglutinin Antibodies, Compositions And Related Methods
  • Method Of Meristem Excision And Transformation
  • Method Of Changing Plants' Characters
  • Method For Excision Of Plant Embryos For Transformation
  • Nucleic Acid Sequences Encoding Transcription Factors Regulating Alkaloid Biosynthesis And Their Use In Modifying Plant Metabolism
  • Nucleic Acid Sequences Encoding Transcription Factors Regulating Alkaloid Biosynthesis And Their Use In Modifying Plant Metabolism
  • Nucleic Acid Sequences Encoding Transcription Factors Regulating Alkaloid Biosynthesis And Their Use In Modifying Plant Metabolism
  • Consolidated Pretreatment And Hydrolysis Of Plant Biomass Expressing Cell Wall Degrading Enzymes
  • Reducing Levels Of Nicotinic Alkaloids In Plants
  • System For Expression Of Genes In Plants
  • Altering Lignin And Wood Density
  • Method For Increasing A Nicotinic Alkaloid In A Nicotiana Plant By Introducing A Mutation Into The Gene Encoding The Nbtf7 Transcription Factor And Plants And Products Made By Said Method
  • Trypanosoma Antigens, Vaccine Compositions, And Related Methods
  • Reducing Levels Of Nicotinic Alkaloids In Plants
  • Compositions And Methods For Improved Protein Production
  • Use Of Non-Agrobacterium Bacterial Species For Plant Transformation
  • Transgenic Plants Expressing Civps Or Intein Modified Proteins And Related Method
  • Compositions And Methods For Custom Site-Specific Dna Recombinases
  • Method For Excision Of Plant Embryos For Transformation
  • System For Expression Of Genes In Plants
  • Process Of Transfecting Plants
  • Transgenic Plants Expressing Civps Or Intein Modified Proteins And Related Method
  • Reducing Levels Of Nicotinic Alkaloids In Plants
  • Method For The Biosynthesis Of Taurine Or Hypotaurine In Cells
  • Transgenic Plants Expressing Civps Or Intein Modified Proteins And Related Method
  • Intein-Modified Enzymes, Their Production And Industrial Applications
  • Transgenic Plants Expressing Civps Or Intein Modified Proteins And Related Method
  • Selection Marker-Free Rhizobiaceae-Mediated Method For Producing A Transgenic Plant Of The Genus Triticum
  • Transgenic Plants Expressing Civps Or Intein Modified Proteins And Related Method
  • Method And Apparatus For Substantially Isolating Plant Tissues
  • Selection Marker - Free Rhizobiaceae - Carrying A Method For The Production Of A Transgenic Plant Of The Species Triticum
  • Transformation Of Mature Citrus
  • Plants With Altered Levels Of Vegetative Starch
  • Method Of Meristem Excision And Transformation
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nature03309

    DOI

    http://dx.doi.org/10.1038/nature03309

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1027789323

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/15703747


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Plant Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Arabidopsis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bacteria", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Biotechnology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA, Bacterial", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Transfer Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Vectors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genotype", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Oryza", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plant Leaves", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plants", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plants, Genetically Modified", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plasmids", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Rhizobium", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Symbiosis", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Tobacco", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transformation, Genetic", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Cambia", 
              "id": "https://www.grid.ac/institutes/grid.499527.4", 
              "name": [
                "CAMBIA (An Affiliated Research Centre of Charles Sturt University), G.P.O. Box 3200, Canberra, ACT 2601, Australia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Broothaerts", 
            "givenName": "Wim", 
            "id": "sg:person.01331714164.26", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01331714164.26"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Cambia", 
              "id": "https://www.grid.ac/institutes/grid.499527.4", 
              "name": [
                "CAMBIA (An Affiliated Research Centre of Charles Sturt University), G.P.O. Box 3200, Canberra, ACT 2601, Australia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mitchell", 
            "givenName": "Heidi J.", 
            "id": "sg:person.01221061617.76", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01221061617.76"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Cambia", 
              "id": "https://www.grid.ac/institutes/grid.499527.4", 
              "name": [
                "CAMBIA (An Affiliated Research Centre of Charles Sturt University), G.P.O. Box 3200, Canberra, ACT 2601, Australia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Weir", 
            "givenName": "Brian", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Cambia", 
              "id": "https://www.grid.ac/institutes/grid.499527.4", 
              "name": [
                "CAMBIA (An Affiliated Research Centre of Charles Sturt University), G.P.O. Box 3200, Canberra, ACT 2601, Australia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kaines", 
            "givenName": "Sarah", 
            "id": "sg:person.0652054016.56", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0652054016.56"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Cambia", 
              "id": "https://www.grid.ac/institutes/grid.499527.4", 
              "name": [
                "CAMBIA (An Affiliated Research Centre of Charles Sturt University), G.P.O. Box 3200, Canberra, ACT 2601, Australia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Smith", 
            "givenName": "Leon M. A.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Cambia", 
              "id": "https://www.grid.ac/institutes/grid.499527.4", 
              "name": [
                "CAMBIA (An Affiliated Research Centre of Charles Sturt University), G.P.O. Box 3200, Canberra, ACT 2601, Australia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yang", 
            "givenName": "Wei", 
            "id": "sg:person.01047022533.53", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047022533.53"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Cambia", 
              "id": "https://www.grid.ac/institutes/grid.499527.4", 
              "name": [
                "CAMBIA (An Affiliated Research Centre of Charles Sturt University), G.P.O. Box 3200, Canberra, ACT 2601, Australia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mayer", 
            "givenName": "Jorge E.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Cambia", 
              "id": "https://www.grid.ac/institutes/grid.499527.4", 
              "name": [
                "CAMBIA (An Affiliated Research Centre of Charles Sturt University), G.P.O. Box 3200, Canberra, ACT 2601, Australia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Roa-Rodr\u00edguez", 
            "givenName": "Carolina", 
            "id": "sg:person.01102531016.34", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102531016.34"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Cambia", 
              "id": "https://www.grid.ac/institutes/grid.499527.4", 
              "name": [
                "CAMBIA (An Affiliated Research Centre of Charles Sturt University), G.P.O. Box 3200, Canberra, ACT 2601, Australia"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jefferson", 
            "givenName": "Richard A.", 
            "id": "sg:person.012224403551.53", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012224403551.53"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/bf01977351", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000235724", 
              "https://doi.org/10.1007/bf01977351"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01977351", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000235724", 
              "https://doi.org/10.1007/bf01977351"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.phyto.39.1.27", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007790329"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00277546", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012920318", 
              "https://doi.org/10.1007/bf00277546"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00277546", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012920318", 
              "https://doi.org/10.1007/bf00277546"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02772830", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013512451", 
              "https://doi.org/10.1007/bf02772830"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-313x.1998.00343.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013777935"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/431494a", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018297747", 
              "https://doi.org/10.1038/431494a"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/431494a", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018297747", 
              "https://doi.org/10.1038/431494a"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.191349198", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019506454"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-313x.1994.6020271.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023112743"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-1-4757-9357-4_6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028731204", 
              "https://doi.org/10.1007/978-1-4757-9357-4_6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.103.030262", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030660053"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrg1000", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032410834", 
              "https://doi.org/10.1038/nrg1000"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrg1000", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032410834", 
              "https://doi.org/10.1038/nrg1000"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0076-6879(02)58106-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033415598"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.182432999", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038426679"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mmbr.67.1.16-37.2003", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040290472"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1360-1385(03)00160-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041482271"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s1360-1385(03)00160-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041482271"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.222228199", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041652911"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1066803", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041672165"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-313x.2002.01468.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044026677"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1094/mpmi-1-231", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060077995"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1094/mpmi.1999.12.4.293", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060080067"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1099/00221287-98-2-477", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060376102"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1099/ijs.0.02762-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060383906"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1060966", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062445052"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.1066804", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062445641"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1075444395", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1077158173", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2005-02", 
        "datePublishedReg": "2005-02-01", 
        "description": "Agrobacterium is widely considered to be the only bacterial genus capable of transferring genes to plants. When suitably modified, Agrobacterium has become the most effective vector for gene transfer in plant biotechnology. However, the complexity of the patent landscape has created both real and perceived obstacles to the effective use of this technology for agricultural improvements by many public and private organizations worldwide. Here we show that several species of bacteria outside the Agrobacterium genus can be modified to mediate gene transfer to a number of diverse plants. These plant-associated symbiotic bacteria were made competent for gene transfer by acquisition of both a disarmed Ti plasmid and a suitable binary vector. This alternative to Agrobacterium-mediated technology for crop improvement, in addition to affording a versatile 'open source' platform for plant biotechnology, may lead to new uses of natural bacteria-plant interactions to achieve plant transformation.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/nature03309", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1018957", 
            "issn": [
              "0090-0028", 
              "1476-4687"
            ], 
            "name": "Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "7026", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "433"
          }
        ], 
        "name": "Gene transfer to plants by diverse species of bacteria", 
        "pagination": "629", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "84617e86e72277220fb56cad75ab15224f40e07f5ad5cde3ee086c43fa60fb60"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "15703747"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "0410462"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/nature03309"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1027789323"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/nature03309", 
          "https://app.dimensions.ai/details/publication/pub.1027789323"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T13:10", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000367_0000000367/records_88247_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://www.nature.com/articles/nature03309"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature03309'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature03309'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature03309'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature03309'


     

    This table displays all metadata directly associated to this object as RDF triples.

    272 TRIPLES      21 PREDICATES      72 URIs      38 LITERALS      26 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/nature03309 schema:about N007b48e7d6db42cebdcea426a2b40e52
    2 N0f852aa0501c4c648289d365580bd28d
    3 N1c390dade4a24396986c7cac5d734ad5
    4 N2313b2fd556e4e019af37091eb17d5e7
    5 N30f733d010dd4fbe969057d6413e4d0a
    6 N449fe1ce87e34e6b9fd60a91e96041a9
    7 N460cf01ff2594523b195aff7d3417817
    8 N4613a687752645f3a9b6efb7f1e478db
    9 N66ac8fe970ae4671b793afc06f6518ba
    10 N6c3fdd33d2fb43db9f4f205fc7759673
    11 N7801496bf2464a11abda1b8a4b03de28
    12 N8a807d4caecc4b4b9c0d6530128f2228
    13 N9198760fdd3343409394ad39f1d2d848
    14 N9ca82f40d9324620b794acd1f939bba4
    15 Na09b7cfdc9b24f55b73b0ff9ea5e4ef4
    16 Na0b35eeda1e24370a8c5413ff072108b
    17 Nea2014fdd62f42a989f3a1695d9c8203
    18 anzsrc-for:06
    19 anzsrc-for:0607
    20 schema:author Nf6150a1f7382478084cf11bbcf888a1d
    21 schema:citation sg:pub.10.1007/978-1-4757-9357-4_6
    22 sg:pub.10.1007/bf00277546
    23 sg:pub.10.1007/bf01977351
    24 sg:pub.10.1007/bf02772830
    25 sg:pub.10.1038/431494a
    26 sg:pub.10.1038/nrg1000
    27 https://app.dimensions.ai/details/publication/pub.1075444395
    28 https://app.dimensions.ai/details/publication/pub.1077158173
    29 https://doi.org/10.1016/s0076-6879(02)58106-4
    30 https://doi.org/10.1016/s1360-1385(03)00160-2
    31 https://doi.org/10.1046/j.1365-313x.1994.6020271.x
    32 https://doi.org/10.1046/j.1365-313x.1998.00343.x
    33 https://doi.org/10.1046/j.1365-313x.2002.01468.x
    34 https://doi.org/10.1073/pnas.182432999
    35 https://doi.org/10.1073/pnas.191349198
    36 https://doi.org/10.1073/pnas.222228199
    37 https://doi.org/10.1094/mpmi-1-231
    38 https://doi.org/10.1094/mpmi.1999.12.4.293
    39 https://doi.org/10.1099/00221287-98-2-477
    40 https://doi.org/10.1099/ijs.0.02762-0
    41 https://doi.org/10.1104/pp.103.030262
    42 https://doi.org/10.1126/science.1060966
    43 https://doi.org/10.1126/science.1066803
    44 https://doi.org/10.1126/science.1066804
    45 https://doi.org/10.1128/mmbr.67.1.16-37.2003
    46 https://doi.org/10.1146/annurev.phyto.39.1.27
    47 schema:datePublished 2005-02
    48 schema:datePublishedReg 2005-02-01
    49 schema:description Agrobacterium is widely considered to be the only bacterial genus capable of transferring genes to plants. When suitably modified, Agrobacterium has become the most effective vector for gene transfer in plant biotechnology. However, the complexity of the patent landscape has created both real and perceived obstacles to the effective use of this technology for agricultural improvements by many public and private organizations worldwide. Here we show that several species of bacteria outside the Agrobacterium genus can be modified to mediate gene transfer to a number of diverse plants. These plant-associated symbiotic bacteria were made competent for gene transfer by acquisition of both a disarmed Ti plasmid and a suitable binary vector. This alternative to Agrobacterium-mediated technology for crop improvement, in addition to affording a versatile 'open source' platform for plant biotechnology, may lead to new uses of natural bacteria-plant interactions to achieve plant transformation.
    50 schema:genre research_article
    51 schema:inLanguage en
    52 schema:isAccessibleForFree false
    53 schema:isPartOf N01369dd1a48b4f529d26fcfb08f3fecd
    54 N3eb3130c0e5f4c9a84be2d68c4f65c40
    55 sg:journal.1018957
    56 schema:name Gene transfer to plants by diverse species of bacteria
    57 schema:pagination 629
    58 schema:productId N1d3b6c002c264b9ea277ddf67de23479
    59 N38c5fa25e97a491ab9a4ecd70e6d1566
    60 N437711fad974453dae0a1b63938e23d2
    61 N6428d29acb064a9baf8dd9770cb1294a
    62 Ncd91f5a32d84452daa5a009c60d10388
    63 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027789323
    64 https://doi.org/10.1038/nature03309
    65 schema:sdDatePublished 2019-04-11T13:10
    66 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    67 schema:sdPublisher Nbe4ca73a4ebf4370a553457ee5160e63
    68 schema:url https://www.nature.com/articles/nature03309
    69 sgo:license sg:explorer/license/
    70 sgo:sdDataset articles
    71 rdf:type schema:ScholarlyArticle
    72 N007b48e7d6db42cebdcea426a2b40e52 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    73 schema:name Plant Leaves
    74 rdf:type schema:DefinedTerm
    75 N01369dd1a48b4f529d26fcfb08f3fecd schema:volumeNumber 433
    76 rdf:type schema:PublicationVolume
    77 N0f852aa0501c4c648289d365580bd28d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    78 schema:name Rhizobium
    79 rdf:type schema:DefinedTerm
    80 N1c390dade4a24396986c7cac5d734ad5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    81 schema:name Base Sequence
    82 rdf:type schema:DefinedTerm
    83 N1d3b6c002c264b9ea277ddf67de23479 schema:name readcube_id
    84 schema:value 84617e86e72277220fb56cad75ab15224f40e07f5ad5cde3ee086c43fa60fb60
    85 rdf:type schema:PropertyValue
    86 N2313b2fd556e4e019af37091eb17d5e7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    87 schema:name Tobacco
    88 rdf:type schema:DefinedTerm
    89 N2aed889da05c400882f97706339692b8 schema:affiliation https://www.grid.ac/institutes/grid.499527.4
    90 schema:familyName Mayer
    91 schema:givenName Jorge E.
    92 rdf:type schema:Person
    93 N30f733d010dd4fbe969057d6413e4d0a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    94 schema:name Arabidopsis
    95 rdf:type schema:DefinedTerm
    96 N38c5fa25e97a491ab9a4ecd70e6d1566 schema:name doi
    97 schema:value 10.1038/nature03309
    98 rdf:type schema:PropertyValue
    99 N39692367688347d68d997f3dc79d329d rdf:first sg:person.01221061617.76
    100 rdf:rest Na1c56521fb7243c4bfc5de6c74105c74
    101 N3eb3130c0e5f4c9a84be2d68c4f65c40 schema:issueNumber 7026
    102 rdf:type schema:PublicationIssue
    103 N437711fad974453dae0a1b63938e23d2 schema:name dimensions_id
    104 schema:value pub.1027789323
    105 rdf:type schema:PropertyValue
    106 N449fe1ce87e34e6b9fd60a91e96041a9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    107 schema:name Symbiosis
    108 rdf:type schema:DefinedTerm
    109 N460cf01ff2594523b195aff7d3417817 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name DNA, Bacterial
    111 rdf:type schema:DefinedTerm
    112 N4613a687752645f3a9b6efb7f1e478db schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    113 schema:name Gene Transfer Techniques
    114 rdf:type schema:DefinedTerm
    115 N53d51be37fd74630821beead7a128d58 rdf:first N96a9f862a78d476aa3a0b79c9c14a648
    116 rdf:rest N61582e3bbe0542209bfc9ff82a61648a
    117 N61582e3bbe0542209bfc9ff82a61648a rdf:first sg:person.01047022533.53
    118 rdf:rest N7ae38408268d407e9c953f4ff17ca7be
    119 N6428d29acb064a9baf8dd9770cb1294a schema:name nlm_unique_id
    120 schema:value 0410462
    121 rdf:type schema:PropertyValue
    122 N66ac8fe970ae4671b793afc06f6518ba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    123 schema:name Transformation, Genetic
    124 rdf:type schema:DefinedTerm
    125 N6c3fdd33d2fb43db9f4f205fc7759673 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    126 schema:name Genotype
    127 rdf:type schema:DefinedTerm
    128 N7801496bf2464a11abda1b8a4b03de28 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    129 schema:name Genetic Vectors
    130 rdf:type schema:DefinedTerm
    131 N7ae38408268d407e9c953f4ff17ca7be rdf:first N2aed889da05c400882f97706339692b8
    132 rdf:rest N9bc8ebed8d46439db522bf19a4fe3810
    133 N8a807d4caecc4b4b9c0d6530128f2228 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    134 schema:name Plants, Genetically Modified
    135 rdf:type schema:DefinedTerm
    136 N9198760fdd3343409394ad39f1d2d848 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    137 schema:name Plants
    138 rdf:type schema:DefinedTerm
    139 N96509444e92b4aeca8e1b885168be517 rdf:first sg:person.012224403551.53
    140 rdf:rest rdf:nil
    141 N96a9f862a78d476aa3a0b79c9c14a648 schema:affiliation https://www.grid.ac/institutes/grid.499527.4
    142 schema:familyName Smith
    143 schema:givenName Leon M. A.
    144 rdf:type schema:Person
    145 N9bc8ebed8d46439db522bf19a4fe3810 rdf:first sg:person.01102531016.34
    146 rdf:rest N96509444e92b4aeca8e1b885168be517
    147 N9ca82f40d9324620b794acd1f939bba4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    148 schema:name Oryza
    149 rdf:type schema:DefinedTerm
    150 Na09b7cfdc9b24f55b73b0ff9ea5e4ef4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    151 schema:name Biotechnology
    152 rdf:type schema:DefinedTerm
    153 Na0b35eeda1e24370a8c5413ff072108b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    154 schema:name Plasmids
    155 rdf:type schema:DefinedTerm
    156 Na1c56521fb7243c4bfc5de6c74105c74 rdf:first Nd8f219863e0a4fd69a7292b2f22b81a8
    157 rdf:rest Ncdeede6e510742c98a3e70bee6c29e9c
    158 Nbe4ca73a4ebf4370a553457ee5160e63 schema:name Springer Nature - SN SciGraph project
    159 rdf:type schema:Organization
    160 Ncd91f5a32d84452daa5a009c60d10388 schema:name pubmed_id
    161 schema:value 15703747
    162 rdf:type schema:PropertyValue
    163 Ncdeede6e510742c98a3e70bee6c29e9c rdf:first sg:person.0652054016.56
    164 rdf:rest N53d51be37fd74630821beead7a128d58
    165 Nd8f219863e0a4fd69a7292b2f22b81a8 schema:affiliation https://www.grid.ac/institutes/grid.499527.4
    166 schema:familyName Weir
    167 schema:givenName Brian
    168 rdf:type schema:Person
    169 Nea2014fdd62f42a989f3a1695d9c8203 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    170 schema:name Bacteria
    171 rdf:type schema:DefinedTerm
    172 Nf6150a1f7382478084cf11bbcf888a1d rdf:first sg:person.01331714164.26
    173 rdf:rest N39692367688347d68d997f3dc79d329d
    174 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    175 schema:name Biological Sciences
    176 rdf:type schema:DefinedTerm
    177 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
    178 schema:name Plant Biology
    179 rdf:type schema:DefinedTerm
    180 sg:journal.1018957 schema:issn 0090-0028
    181 1476-4687
    182 schema:name Nature
    183 rdf:type schema:Periodical
    184 sg:person.01047022533.53 schema:affiliation https://www.grid.ac/institutes/grid.499527.4
    185 schema:familyName Yang
    186 schema:givenName Wei
    187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047022533.53
    188 rdf:type schema:Person
    189 sg:person.01102531016.34 schema:affiliation https://www.grid.ac/institutes/grid.499527.4
    190 schema:familyName Roa-Rodríguez
    191 schema:givenName Carolina
    192 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102531016.34
    193 rdf:type schema:Person
    194 sg:person.01221061617.76 schema:affiliation https://www.grid.ac/institutes/grid.499527.4
    195 schema:familyName Mitchell
    196 schema:givenName Heidi J.
    197 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01221061617.76
    198 rdf:type schema:Person
    199 sg:person.012224403551.53 schema:affiliation https://www.grid.ac/institutes/grid.499527.4
    200 schema:familyName Jefferson
    201 schema:givenName Richard A.
    202 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012224403551.53
    203 rdf:type schema:Person
    204 sg:person.01331714164.26 schema:affiliation https://www.grid.ac/institutes/grid.499527.4
    205 schema:familyName Broothaerts
    206 schema:givenName Wim
    207 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01331714164.26
    208 rdf:type schema:Person
    209 sg:person.0652054016.56 schema:affiliation https://www.grid.ac/institutes/grid.499527.4
    210 schema:familyName Kaines
    211 schema:givenName Sarah
    212 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0652054016.56
    213 rdf:type schema:Person
    214 sg:pub.10.1007/978-1-4757-9357-4_6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028731204
    215 https://doi.org/10.1007/978-1-4757-9357-4_6
    216 rdf:type schema:CreativeWork
    217 sg:pub.10.1007/bf00277546 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012920318
    218 https://doi.org/10.1007/bf00277546
    219 rdf:type schema:CreativeWork
    220 sg:pub.10.1007/bf01977351 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000235724
    221 https://doi.org/10.1007/bf01977351
    222 rdf:type schema:CreativeWork
    223 sg:pub.10.1007/bf02772830 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013512451
    224 https://doi.org/10.1007/bf02772830
    225 rdf:type schema:CreativeWork
    226 sg:pub.10.1038/431494a schema:sameAs https://app.dimensions.ai/details/publication/pub.1018297747
    227 https://doi.org/10.1038/431494a
    228 rdf:type schema:CreativeWork
    229 sg:pub.10.1038/nrg1000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032410834
    230 https://doi.org/10.1038/nrg1000
    231 rdf:type schema:CreativeWork
    232 https://app.dimensions.ai/details/publication/pub.1075444395 schema:CreativeWork
    233 https://app.dimensions.ai/details/publication/pub.1077158173 schema:CreativeWork
    234 https://doi.org/10.1016/s0076-6879(02)58106-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033415598
    235 rdf:type schema:CreativeWork
    236 https://doi.org/10.1016/s1360-1385(03)00160-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041482271
    237 rdf:type schema:CreativeWork
    238 https://doi.org/10.1046/j.1365-313x.1994.6020271.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023112743
    239 rdf:type schema:CreativeWork
    240 https://doi.org/10.1046/j.1365-313x.1998.00343.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1013777935
    241 rdf:type schema:CreativeWork
    242 https://doi.org/10.1046/j.1365-313x.2002.01468.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1044026677
    243 rdf:type schema:CreativeWork
    244 https://doi.org/10.1073/pnas.182432999 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038426679
    245 rdf:type schema:CreativeWork
    246 https://doi.org/10.1073/pnas.191349198 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019506454
    247 rdf:type schema:CreativeWork
    248 https://doi.org/10.1073/pnas.222228199 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041652911
    249 rdf:type schema:CreativeWork
    250 https://doi.org/10.1094/mpmi-1-231 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060077995
    251 rdf:type schema:CreativeWork
    252 https://doi.org/10.1094/mpmi.1999.12.4.293 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060080067
    253 rdf:type schema:CreativeWork
    254 https://doi.org/10.1099/00221287-98-2-477 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060376102
    255 rdf:type schema:CreativeWork
    256 https://doi.org/10.1099/ijs.0.02762-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060383906
    257 rdf:type schema:CreativeWork
    258 https://doi.org/10.1104/pp.103.030262 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030660053
    259 rdf:type schema:CreativeWork
    260 https://doi.org/10.1126/science.1060966 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062445052
    261 rdf:type schema:CreativeWork
    262 https://doi.org/10.1126/science.1066803 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041672165
    263 rdf:type schema:CreativeWork
    264 https://doi.org/10.1126/science.1066804 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062445641
    265 rdf:type schema:CreativeWork
    266 https://doi.org/10.1128/mmbr.67.1.16-37.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040290472
    267 rdf:type schema:CreativeWork
    268 https://doi.org/10.1146/annurev.phyto.39.1.27 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007790329
    269 rdf:type schema:CreativeWork
    270 https://www.grid.ac/institutes/grid.499527.4 schema:alternateName Cambia
    271 schema:name CAMBIA (An Affiliated Research Centre of Charles Sturt University), G.P.O. Box 3200, Canberra, ACT 2601, Australia
    272 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...