Accurate multiplex gene synthesis from programmable DNA microchips View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2004-12

AUTHORS

Jingdong Tian, Hui Gong, Nijing Sheng, Xiaochuan Zhou, Erdogan Gulari, Xiaolian Gao, George Church

ABSTRACT

Testing the many hypotheses from genomics and systems biology experiments demands accurate and cost-effective gene and genome synthesis. Here we describe a microchip-based technology for multiplex gene synthesis. Pools of thousands of 'construction' oligonucleotides and tagged complementary 'selection' oligonucleotides are synthesized on photo-programmable microfluidic chips, released, amplified and selected by hybridization to reduce synthesis errors ninefold. A one-step polymerase assembly multiplexing reaction assembles these into multiple genes. This technology enabled us to synthesize all 21 genes that encode the proteins of the Escherichia coli 30S ribosomal subunit, and to optimize their translation efficiency in vitro through alteration of codon bias. This is a significant step towards the synthesis of ribosomes in vitro and should have utility for synthetic biology in general. More... »

PAGES

1050

References to SciGraph publications

Journal

TITLE

Nature

ISSUE

7020

VOLUME

432

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nature03151

    DOI

    http://dx.doi.org/10.1038/nature03151

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1052700485

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/15616567


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