Processing of primary microRNAs by the Microprocessor complex View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2004-11

AUTHORS

Ahmet M. Denli, Bastiaan B. J. Tops, Ronald H. A. Plasterk, René F. Ketting, Gregory J. Hannon

ABSTRACT

Mature microRNAs (miRNAs) are generated via a two-step processing pathway to yield approximately 22-nucleotide small RNAs that regulate gene expression at the post-transcriptional level. Initial cleavage is catalysed by Drosha, a nuclease of the RNase III family, which acts on primary miRNA transcripts (pri-miRNAs) in the nucleus. Here we show that Drosha exists in a multiprotein complex, the Microprocessor, and begin the process of deconstructing that complex into its constituent components. Along with Drosha, the Microprocessor also contains Pasha (partner of Drosha), a double-stranded RNA binding protein. Suppression of Pasha expression in Drosophila cells or Caenorhabditis elegans interferes with pri-miRNA processing, leading to an accumulation of pri-miRNAs and a reduction in mature miRNAs. Finally, depletion or mutation of pash-1 in C. elegans causes de-repression of a let-7 reporter and the appearance of phenotypic defects overlapping those observed upon examination of worms with lesions in Dicer (dcr-1) or Drosha (drsh-1). Considered together, these results indicate a role for Pasha in miRNA maturation and miRNA-mediated gene regulation. More... »

PAGES

231

Journal

TITLE

Nature

ISSUE

7014

VOLUME

432

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nature03049

DOI

http://dx.doi.org/10.1038/nature03049

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027442042

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15531879


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Caenorhabditis elegans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Caenorhabditis elegans Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drosophila Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drosophila melanogaster", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Endoribonucleases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Reporter", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "MicroRNAs", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Multiprotein Complexes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phenotype", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Binding", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Structure, Tertiary", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA Processing, Post-Transcriptional", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA-Binding Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ribonuclease III", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Cold Spring Harbor Laboratory", 
          "id": "https://www.grid.ac/institutes/grid.225279.9", 
          "name": [
            "Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Denli", 
        "givenName": "Ahmet M.", 
        "id": "sg:person.01270525120.76", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270525120.76"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "The Hubrecht Laboratory Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tops", 
        "givenName": "Bastiaan B. J.", 
        "id": "sg:person.014606253407.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014606253407.10"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "The Hubrecht Laboratory Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Plasterk", 
        "givenName": "Ronald H. A.", 
        "id": "sg:person.01230321605.78", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230321605.78"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "The Hubrecht Laboratory Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ketting", 
        "givenName": "Ren\u00e9 F.", 
        "id": "sg:person.0724156727.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0724156727.17"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Cold Spring Harbor Laboratory", 
          "id": "https://www.grid.ac/institutes/grid.225279.9", 
          "name": [
            "Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hannon", 
        "givenName": "Gregory J.", 
        "id": "sg:person.015076500737.82", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015076500737.82"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1126/science.1090599", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003575931"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.927801", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006489317"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/cdf476", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007150338"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1261/rna.5167604", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007798221"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01957", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007929702", 
          "https://doi.org/10.1038/nature01957"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01957", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007929702", 
          "https://doi.org/10.1038/nature01957"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(03)00759-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008095364"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-4827(86)90560-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009254855"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m005494200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011096271"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-291x(03)00554-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012591222"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-291x(03)00554-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012591222"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature03120", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015411207", 
          "https://doi.org/10.1038/nature03120"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature03120", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015411207", 
          "https://doi.org/10.1038/nature03120"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.1025202", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019149124"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1097-2765(02)00541-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019720069"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsb1016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022590182", 
          "https://doi.org/10.1038/nsb1016"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsb1016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022590182", 
          "https://doi.org/10.1038/nsb1016"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(02)00793-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025714549"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1090289", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029676590"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01956", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030197667", 
          "https://doi.org/10.1038/nature01956"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature01956", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030197667", 
          "https://doi.org/10.1038/nature01956"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0307969100", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034532156"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(01)00431-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036555460"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35053110", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042658253", 
          "https://doi.org/10.1038/35053110"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35053110", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042658253", 
          "https://doi.org/10.1038/35053110"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/dbio.1999.9523", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045998074"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35002607", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047689840", 
          "https://doi.org/10.1038/35002607"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35002607", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047689840", 
          "https://doi.org/10.1038/35002607"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2004.06.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047794235"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.1158803", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049322291"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1062961", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049854130"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.1064703", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052230676"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1076311", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062446946"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1085242", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062448182"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1088710", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062448487"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1097434", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062449602"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2004-11", 
    "datePublishedReg": "2004-11-01", 
    "description": "Mature microRNAs (miRNAs) are generated via a two-step processing pathway to yield approximately 22-nucleotide small RNAs that regulate gene expression at the post-transcriptional level. Initial cleavage is catalysed by Drosha, a nuclease of the RNase III family, which acts on primary miRNA transcripts (pri-miRNAs) in the nucleus. Here we show that Drosha exists in a multiprotein complex, the Microprocessor, and begin the process of deconstructing that complex into its constituent components. Along with Drosha, the Microprocessor also contains Pasha (partner of Drosha), a double-stranded RNA binding protein. Suppression of Pasha expression in Drosophila cells or Caenorhabditis elegans interferes with pri-miRNA processing, leading to an accumulation of pri-miRNAs and a reduction in mature miRNAs. Finally, depletion or mutation of pash-1 in C. elegans causes de-repression of a let-7 reporter and the appearance of phenotypic defects overlapping those observed upon examination of worms with lesions in Dicer (dcr-1) or Drosha (drsh-1). Considered together, these results indicate a role for Pasha in miRNA maturation and miRNA-mediated gene regulation.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nature03049", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7014", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "432"
      }
    ], 
    "name": "Processing of primary microRNAs by the Microprocessor complex", 
    "pagination": "231", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "82b967c237b006bf411f33391f5cda02c7328c1936c5417080eca33bba7e6381"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "15531879"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nature03049"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1027442042"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nature03049", 
      "https://app.dimensions.ai/details/publication/pub.1027442042"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:26", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000362_0000000362/records_87109_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/nature03049"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature03049'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature03049'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature03049'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature03049'


 

This table displays all metadata directly associated to this object as RDF triples.

264 TRIPLES      21 PREDICATES      75 URIs      38 LITERALS      26 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nature03049 schema:about N083fc083c9064fdd83c5f9051b9d536f
2 N1c7fe4f42521478c89c08d59ffecf65b
3 N203488b525f94003bcf4c13d509eae17
4 N3a98f494004544bb9ae47ec07eb3dd56
5 N3f157c74df94496eb6b2f0e7901b1ffc
6 N420cea30772946229684ffd2763fe4b1
7 N4709afe0d4864dc6803cc6e52cd4c0cc
8 N5499ba0f4e9149d68b3b36193708d875
9 N5e1b09933bbc4054b66d5a023b04bfc6
10 N7529a028325e492da2e755d1f7d59937
11 N7e3ab933a37c41c7a67ab70c47d4c9a7
12 N8b1bac9d74464839a4d1971cf21f8216
13 Nade0248b08444c07bed1119c5aa64313
14 Nb458d303545d43ec90ca52e531e98023
15 Nb8c9d5861bbc4511bc5300a6882c1465
16 Nb8cdc588dcce4c5790e2a408a0a1f28e
17 Nf446edabe55544a08fde97f62ac8dc5c
18 anzsrc-for:06
19 anzsrc-for:0604
20 schema:author N957553ff02d7474d8bf8e00d0498891e
21 schema:citation sg:pub.10.1038/35002607
22 sg:pub.10.1038/35053110
23 sg:pub.10.1038/nature01956
24 sg:pub.10.1038/nature01957
25 sg:pub.10.1038/nature03120
26 sg:pub.10.1038/nsb1016
27 https://doi.org/10.1006/dbio.1999.9523
28 https://doi.org/10.1016/0014-4827(86)90560-4
29 https://doi.org/10.1016/j.cell.2004.06.017
30 https://doi.org/10.1016/s0006-291x(03)00554-0
31 https://doi.org/10.1016/s0092-8674(01)00431-7
32 https://doi.org/10.1016/s0092-8674(02)00793-6
33 https://doi.org/10.1016/s0092-8674(03)00759-1
34 https://doi.org/10.1016/s1097-2765(02)00541-5
35 https://doi.org/10.1073/pnas.0307969100
36 https://doi.org/10.1074/jbc.m005494200
37 https://doi.org/10.1093/emboj/cdf476
38 https://doi.org/10.1101/gad.1025202
39 https://doi.org/10.1101/gad.1064703
40 https://doi.org/10.1101/gad.1158803
41 https://doi.org/10.1101/gad.927801
42 https://doi.org/10.1126/science.1062961
43 https://doi.org/10.1126/science.1076311
44 https://doi.org/10.1126/science.1085242
45 https://doi.org/10.1126/science.1088710
46 https://doi.org/10.1126/science.1090289
47 https://doi.org/10.1126/science.1090599
48 https://doi.org/10.1126/science.1097434
49 https://doi.org/10.1261/rna.5167604
50 schema:datePublished 2004-11
51 schema:datePublishedReg 2004-11-01
52 schema:description Mature microRNAs (miRNAs) are generated via a two-step processing pathway to yield approximately 22-nucleotide small RNAs that regulate gene expression at the post-transcriptional level. Initial cleavage is catalysed by Drosha, a nuclease of the RNase III family, which acts on primary miRNA transcripts (pri-miRNAs) in the nucleus. Here we show that Drosha exists in a multiprotein complex, the Microprocessor, and begin the process of deconstructing that complex into its constituent components. Along with Drosha, the Microprocessor also contains Pasha (partner of Drosha), a double-stranded RNA binding protein. Suppression of Pasha expression in Drosophila cells or Caenorhabditis elegans interferes with pri-miRNA processing, leading to an accumulation of pri-miRNAs and a reduction in mature miRNAs. Finally, depletion or mutation of pash-1 in C. elegans causes de-repression of a let-7 reporter and the appearance of phenotypic defects overlapping those observed upon examination of worms with lesions in Dicer (dcr-1) or Drosha (drsh-1). Considered together, these results indicate a role for Pasha in miRNA maturation and miRNA-mediated gene regulation.
53 schema:genre research_article
54 schema:inLanguage en
55 schema:isAccessibleForFree false
56 schema:isPartOf N071e295348504356a07db0c64dd117c3
57 N69c9b7af922146699863c4f85ea967bc
58 sg:journal.1018957
59 schema:name Processing of primary microRNAs by the Microprocessor complex
60 schema:pagination 231
61 schema:productId N592808fbe55147548d5867a33ca627e4
62 Na023ce1c2eeb4eaea9a62879ee5c442d
63 Ncc5f8e7d7ab74680afbccc5e7d2bd8aa
64 Nd817ecb6afb94bf6b3887271f30f5c47
65 Ne0ed1efbb3044cccafb5c5e4346fce6b
66 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027442042
67 https://doi.org/10.1038/nature03049
68 schema:sdDatePublished 2019-04-11T12:26
69 schema:sdLicense https://scigraph.springernature.com/explorer/license/
70 schema:sdPublisher N35f086dd84414ce7b7efa9fa71976543
71 schema:url https://www.nature.com/articles/nature03049
72 sgo:license sg:explorer/license/
73 sgo:sdDataset articles
74 rdf:type schema:ScholarlyArticle
75 N071e295348504356a07db0c64dd117c3 schema:issueNumber 7014
76 rdf:type schema:PublicationIssue
77 N083fc083c9064fdd83c5f9051b9d536f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
78 schema:name Protein Structure, Tertiary
79 rdf:type schema:DefinedTerm
80 N1c7fe4f42521478c89c08d59ffecf65b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name Multiprotein Complexes
82 rdf:type schema:DefinedTerm
83 N203488b525f94003bcf4c13d509eae17 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name MicroRNAs
85 rdf:type schema:DefinedTerm
86 N35f086dd84414ce7b7efa9fa71976543 schema:name Springer Nature - SN SciGraph project
87 rdf:type schema:Organization
88 N36a74b7686f8486c8b15abd419e74a1c rdf:first sg:person.014606253407.10
89 rdf:rest N933cea1df11c4f48b04f20dde352ae71
90 N3a98f494004544bb9ae47ec07eb3dd56 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name RNA-Binding Proteins
92 rdf:type schema:DefinedTerm
93 N3f157c74df94496eb6b2f0e7901b1ffc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Caenorhabditis elegans
95 rdf:type schema:DefinedTerm
96 N420cea30772946229684ffd2763fe4b1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Endoribonucleases
98 rdf:type schema:DefinedTerm
99 N4709afe0d4864dc6803cc6e52cd4c0cc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Phenotype
101 rdf:type schema:DefinedTerm
102 N5499ba0f4e9149d68b3b36193708d875 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Caenorhabditis elegans Proteins
104 rdf:type schema:DefinedTerm
105 N592808fbe55147548d5867a33ca627e4 schema:name dimensions_id
106 schema:value pub.1027442042
107 rdf:type schema:PropertyValue
108 N5e1b09933bbc4054b66d5a023b04bfc6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Ribonuclease III
110 rdf:type schema:DefinedTerm
111 N69c9b7af922146699863c4f85ea967bc schema:volumeNumber 432
112 rdf:type schema:PublicationVolume
113 N74f2643af9a54a12a7818ac20ca82cfe schema:name The Hubrecht Laboratory Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
114 rdf:type schema:Organization
115 N7529a028325e492da2e755d1f7d59937 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Drosophila Proteins
117 rdf:type schema:DefinedTerm
118 N793967ea7b5d4492b20b5dad3bc16375 schema:name The Hubrecht Laboratory Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
119 rdf:type schema:Organization
120 N7e3ab933a37c41c7a67ab70c47d4c9a7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Protein Binding
122 rdf:type schema:DefinedTerm
123 N8b1bac9d74464839a4d1971cf21f8216 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Humans
125 rdf:type schema:DefinedTerm
126 N92a2131698764f0aaa4c13bf06bb7a90 rdf:first sg:person.0724156727.17
127 rdf:rest Na842735f68d743e18b912845ff0f2db4
128 N933cea1df11c4f48b04f20dde352ae71 rdf:first sg:person.01230321605.78
129 rdf:rest N92a2131698764f0aaa4c13bf06bb7a90
130 N957553ff02d7474d8bf8e00d0498891e rdf:first sg:person.01270525120.76
131 rdf:rest N36a74b7686f8486c8b15abd419e74a1c
132 Na023ce1c2eeb4eaea9a62879ee5c442d schema:name pubmed_id
133 schema:value 15531879
134 rdf:type schema:PropertyValue
135 Na842735f68d743e18b912845ff0f2db4 rdf:first sg:person.015076500737.82
136 rdf:rest rdf:nil
137 Nade0248b08444c07bed1119c5aa64313 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name RNA Processing, Post-Transcriptional
139 rdf:type schema:DefinedTerm
140 Nb3c4726ed23e4bcca2c5b25997cd2a6e schema:name The Hubrecht Laboratory Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
141 rdf:type schema:Organization
142 Nb458d303545d43ec90ca52e531e98023 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Drosophila melanogaster
144 rdf:type schema:DefinedTerm
145 Nb8c9d5861bbc4511bc5300a6882c1465 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Cell Line
147 rdf:type schema:DefinedTerm
148 Nb8cdc588dcce4c5790e2a408a0a1f28e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Genes, Reporter
150 rdf:type schema:DefinedTerm
151 Ncc5f8e7d7ab74680afbccc5e7d2bd8aa schema:name nlm_unique_id
152 schema:value 0410462
153 rdf:type schema:PropertyValue
154 Nd817ecb6afb94bf6b3887271f30f5c47 schema:name doi
155 schema:value 10.1038/nature03049
156 rdf:type schema:PropertyValue
157 Ne0ed1efbb3044cccafb5c5e4346fce6b schema:name readcube_id
158 schema:value 82b967c237b006bf411f33391f5cda02c7328c1936c5417080eca33bba7e6381
159 rdf:type schema:PropertyValue
160 Nf446edabe55544a08fde97f62ac8dc5c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
161 schema:name Animals
162 rdf:type schema:DefinedTerm
163 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
164 schema:name Biological Sciences
165 rdf:type schema:DefinedTerm
166 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
167 schema:name Genetics
168 rdf:type schema:DefinedTerm
169 sg:journal.1018957 schema:issn 0090-0028
170 1476-4687
171 schema:name Nature
172 rdf:type schema:Periodical
173 sg:person.01230321605.78 schema:affiliation Nb3c4726ed23e4bcca2c5b25997cd2a6e
174 schema:familyName Plasterk
175 schema:givenName Ronald H. A.
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230321605.78
177 rdf:type schema:Person
178 sg:person.01270525120.76 schema:affiliation https://www.grid.ac/institutes/grid.225279.9
179 schema:familyName Denli
180 schema:givenName Ahmet M.
181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270525120.76
182 rdf:type schema:Person
183 sg:person.014606253407.10 schema:affiliation N74f2643af9a54a12a7818ac20ca82cfe
184 schema:familyName Tops
185 schema:givenName Bastiaan B. J.
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014606253407.10
187 rdf:type schema:Person
188 sg:person.015076500737.82 schema:affiliation https://www.grid.ac/institutes/grid.225279.9
189 schema:familyName Hannon
190 schema:givenName Gregory J.
191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015076500737.82
192 rdf:type schema:Person
193 sg:person.0724156727.17 schema:affiliation N793967ea7b5d4492b20b5dad3bc16375
194 schema:familyName Ketting
195 schema:givenName René F.
196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0724156727.17
197 rdf:type schema:Person
198 sg:pub.10.1038/35002607 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047689840
199 https://doi.org/10.1038/35002607
200 rdf:type schema:CreativeWork
201 sg:pub.10.1038/35053110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042658253
202 https://doi.org/10.1038/35053110
203 rdf:type schema:CreativeWork
204 sg:pub.10.1038/nature01956 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030197667
205 https://doi.org/10.1038/nature01956
206 rdf:type schema:CreativeWork
207 sg:pub.10.1038/nature01957 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007929702
208 https://doi.org/10.1038/nature01957
209 rdf:type schema:CreativeWork
210 sg:pub.10.1038/nature03120 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015411207
211 https://doi.org/10.1038/nature03120
212 rdf:type schema:CreativeWork
213 sg:pub.10.1038/nsb1016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022590182
214 https://doi.org/10.1038/nsb1016
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1006/dbio.1999.9523 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045998074
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1016/0014-4827(86)90560-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009254855
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1016/j.cell.2004.06.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047794235
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1016/s0006-291x(03)00554-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012591222
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1016/s0092-8674(01)00431-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036555460
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1016/s0092-8674(02)00793-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025714549
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1016/s0092-8674(03)00759-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008095364
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1016/s1097-2765(02)00541-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019720069
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1073/pnas.0307969100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034532156
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1074/jbc.m005494200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011096271
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1093/emboj/cdf476 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007150338
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1101/gad.1025202 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019149124
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1101/gad.1064703 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052230676
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1101/gad.1158803 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049322291
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1101/gad.927801 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006489317
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1126/science.1062961 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049854130
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1126/science.1076311 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062446946
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1126/science.1085242 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062448182
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1126/science.1088710 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062448487
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1126/science.1090289 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029676590
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1126/science.1090599 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003575931
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1126/science.1097434 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062449602
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1261/rna.5167604 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007798221
261 rdf:type schema:CreativeWork
262 https://www.grid.ac/institutes/grid.225279.9 schema:alternateName Cold Spring Harbor Laboratory
263 schema:name Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
264 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...