Global analysis of protein localization in budding yeast View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-10

AUTHORS

Won-Ki Huh, James V. Falvo, Luke C. Gerke, Adam S. Carroll, Russell W. Howson, Jonathan S. Weissman, Erin K. O'Shea

ABSTRACT

A fundamental goal of cell biology is to define the functions of proteins in the context of compartments that organize them in the cellular environment. Here we describe the construction and analysis of a collection of yeast strains expressing full-length, chromosomally tagged green fluorescent protein fusion proteins. We classify these proteins, representing 75% of the yeast proteome, into 22 distinct subcellular localization categories, and provide localization information for 70% of previously unlocalized proteins. Analysis of this high-resolution, high-coverage localization data set in the context of transcriptional, genetic, and protein–protein interaction data helps reveal the logic of transcriptional co-regulation, and provides a comprehensive view of interactions within and between organelles in eukaryotic cells. More... »

PAGES

686-691

Journal

TITLE

Nature

ISSUE

6959

VOLUME

425

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nature02026

    DOI

    http://dx.doi.org/10.1038/nature02026

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1026526651

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/14562095


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