Structure and gating mechanism of the acetylcholine receptor pore View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-06

AUTHORS

Atsuo Miyazawa, Yoshinori Fujiyoshi, Nigel Unwin

ABSTRACT

The nicotinic acetylcholine receptor controls electrical signalling between nerve and muscle cells by opening and closing a gated, membrane-spanning pore. Here we present an atomic model of the closed pore, obtained by electron microscopy of crystalline postsynaptic membranes. The pore is shaped by an inner ring of 5 alpha-helices, which curve radially to create a tapering path for the ions, and an outer ring of 15 alpha-helices, which coil around each other and shield the inner ring from the lipids. The gate is a constricting hydrophobic girdle at the middle of the lipid bilayer, formed by weak interactions between neighbouring inner helices. When acetylcholine enters the ligand-binding domain, it triggers rotations of the protein chains on opposite sides of the entrance to the pore. These rotations are communicated through the inner helices, and open the pore by breaking the girdle apart. More... »

PAGES

949

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nature01748

DOI

http://dx.doi.org/10.1038/nature01748

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010350589

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12827192


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Acetylcholine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Crystallization", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Electric Conductivity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ion Channel Gating", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ions", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Models, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Conformation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Folding", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Receptors, Cholinergic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Torpedo", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "*RIKEN Harima Institute, 1-1-1 Kouto, Mikazuki-cho, Sayo, Hyogo 679-5148, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Miyazawa", 
        "givenName": "Atsuo", 
        "id": "sg:person.01265767156.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01265767156.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kyoto University", 
          "id": "https://www.grid.ac/institutes/grid.258799.8", 
          "name": [
            "\u2020Department of Biophysics, Faculty of Science, Kyoto University, Oiwake, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fujiyoshi", 
        "givenName": "Yoshinori", 
        "id": "sg:person.01077633456.74", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01077633456.74"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "MRC Laboratory of Molecular Biology", 
          "id": "https://www.grid.ac/institutes/grid.42475.30", 
          "name": [
            "\u2021MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Unwin", 
        "givenName": "Nigel", 
        "id": "sg:person.0611456533.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611456533.48"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1002/jcc.540120405", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000521002"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0263-7855(93)87009-t", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001021383"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-2836(02)00381-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001869447"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.95.11.6504", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001965255"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/hmg/6.5.767", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002561885"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0021889892009944", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003451988"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/336247a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004057678", 
          "https://doi.org/10.1038/336247a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/336247a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004057678", 
          "https://doi.org/10.1038/336247a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-3495(93)81293-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004280208"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0304-3991(99)00120-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004596493"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-3991(91)90159-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006639320"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-3991(91)90159-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006639320"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0896-6273(94)90257-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011410608"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0108767390010224", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014210169"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0021889891004399", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014885043"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.160128797", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017014142"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.83.8.2719", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019541501"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.99.4.1202", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022508867"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s0365110x58000517", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023091745"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0304-3991(97)00070-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029131989"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/6326", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029776234", 
          "https://doi.org/10.1038/6326"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/6326", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029776234", 
          "https://doi.org/10.1038/6326"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/366479a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030007131", 
          "https://doi.org/10.1038/366479a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-5793(86)80881-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030637209"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(70)90036-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030728416"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.273.15.8659", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031451936"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-3991(92)90003-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034114385"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-3991(92)90003-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034114385"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/335645a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035437234", 
          "https://doi.org/10.1038/335645a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.pharmtox.40.1.431", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035865674"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0896-6273(00)80506-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036914579"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/376514a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037506717", 
          "https://doi.org/10.1038/376514a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1098/rspb.1991.0012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037519871"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0896-6273(90)90445-l", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038680263"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmbi.1999.2721", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041196714"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/324670a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041383088", 
          "https://doi.org/10.1038/324670a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/324670a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041383088", 
          "https://doi.org/10.1038/324670a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.92.3.758", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041527171"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmbi.1993.1107", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042006239"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.82.1.150", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042322385"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrn731", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042688388", 
          "https://doi.org/10.1038/nrn731"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrn731", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042688388", 
          "https://doi.org/10.1038/nrn731"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35077011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044023349", 
          "https://doi.org/10.1038/35077011"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35077011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044023349", 
          "https://doi.org/10.1038/35077011"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-3991(87)90010-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044613010"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-3991(87)90010-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044613010"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1085/jgp.116.3.327", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046349217"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/373037a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050150805", 
          "https://doi.org/10.1038/373037a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/386088a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050523440", 
          "https://doi.org/10.1038/386088a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00176a016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055163894"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi980143m", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055215483"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi980143m", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055215483"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jp012233y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056046871"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jp012233y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056046871"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077171507", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082415541", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2003-06", 
    "datePublishedReg": "2003-06-01", 
    "description": "The nicotinic acetylcholine receptor controls electrical signalling between nerve and muscle cells by opening and closing a gated, membrane-spanning pore. Here we present an atomic model of the closed pore, obtained by electron microscopy of crystalline postsynaptic membranes. The pore is shaped by an inner ring of 5 alpha-helices, which curve radially to create a tapering path for the ions, and an outer ring of 15 alpha-helices, which coil around each other and shield the inner ring from the lipids. The gate is a constricting hydrophobic girdle at the middle of the lipid bilayer, formed by weak interactions between neighbouring inner helices. When acetylcholine enters the ligand-binding domain, it triggers rotations of the protein chains on opposite sides of the entrance to the pore. These rotations are communicated through the inner helices, and open the pore by breaking the girdle apart.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nature01748", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6943", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "423"
      }
    ], 
    "name": "Structure and gating mechanism of the acetylcholine receptor pore", 
    "pagination": "949", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "314faa065ba16be2be94de2fd3656359973ca53fd1eb1496c8f9d2b96e869cde"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "12827192"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nature01748"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1010350589"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nature01748", 
      "https://app.dimensions.ai/details/publication/pub.1010350589"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:26", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000362_0000000362/records_87109_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/nature01748"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature01748'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature01748'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature01748'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature01748'


 

This table displays all metadata directly associated to this object as RDF triples.

286 TRIPLES      21 PREDICATES      88 URIs      34 LITERALS      22 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nature01748 schema:about N14e56e82797940849ba719471a42b4b8
2 N5bee7ad76c0c495198aa4fef5223e60c
3 N8190017f037e45438773f80277a8ffba
4 N96dc0e0554844d9ca814dd99adb90cf6
5 N98dce6526ae74cfd995bffbec709fcbf
6 Nb3adcdceb1f64010b28ba6b620d48f0b
7 Nb9c4a216a0014d8a87cbfe34c2d3f6ab
8 Nbcb0c4088bd14e09a1375f382dc6e912
9 Nc0b06709b483473599f70d1adfe3774d
10 Nc691f0ff2fb246f3a465a0d3f01d1654
11 Ncd2e43743b3f437abd36849302fa96d6
12 Ne2cf0fc833f54b22b634f27cd02243db
13 Neac5ea374b334a86900133a8c7ace537
14 anzsrc-for:06
15 anzsrc-for:0601
16 schema:author N2872f4fe0d984cccb8e6b2f0ce633471
17 schema:citation sg:pub.10.1038/324670a0
18 sg:pub.10.1038/335645a0
19 sg:pub.10.1038/336247a0
20 sg:pub.10.1038/35077011
21 sg:pub.10.1038/366479a0
22 sg:pub.10.1038/373037a0
23 sg:pub.10.1038/376514a0
24 sg:pub.10.1038/386088a0
25 sg:pub.10.1038/6326
26 sg:pub.10.1038/nrn731
27 https://app.dimensions.ai/details/publication/pub.1077171507
28 https://app.dimensions.ai/details/publication/pub.1082415541
29 https://doi.org/10.1002/jcc.540120405
30 https://doi.org/10.1006/jmbi.1993.1107
31 https://doi.org/10.1006/jmbi.1999.2721
32 https://doi.org/10.1016/0014-5793(86)80881-x
33 https://doi.org/10.1016/0022-2836(70)90036-7
34 https://doi.org/10.1016/0263-7855(93)87009-t
35 https://doi.org/10.1016/0304-3991(87)90010-6
36 https://doi.org/10.1016/0304-3991(91)90159-4
37 https://doi.org/10.1016/0304-3991(92)90003-3
38 https://doi.org/10.1016/0896-6273(90)90445-l
39 https://doi.org/10.1016/0896-6273(94)90257-7
40 https://doi.org/10.1016/s0006-3495(93)81293-1
41 https://doi.org/10.1016/s0022-2836(02)00381-9
42 https://doi.org/10.1016/s0304-3991(97)00070-3
43 https://doi.org/10.1016/s0304-3991(99)00120-5
44 https://doi.org/10.1016/s0896-6273(00)80506-1
45 https://doi.org/10.1021/bi00176a016
46 https://doi.org/10.1021/bi980143m
47 https://doi.org/10.1021/jp012233y
48 https://doi.org/10.1073/pnas.160128797
49 https://doi.org/10.1073/pnas.83.8.2719
50 https://doi.org/10.1073/pnas.92.3.758
51 https://doi.org/10.1073/pnas.95.11.6504
52 https://doi.org/10.1074/jbc.273.15.8659
53 https://doi.org/10.1083/jcb.82.1.150
54 https://doi.org/10.1083/jcb.99.4.1202
55 https://doi.org/10.1085/jgp.116.3.327
56 https://doi.org/10.1093/hmg/6.5.767
57 https://doi.org/10.1098/rspb.1991.0012
58 https://doi.org/10.1107/s0021889891004399
59 https://doi.org/10.1107/s0021889892009944
60 https://doi.org/10.1107/s0108767390010224
61 https://doi.org/10.1107/s0365110x58000517
62 https://doi.org/10.1146/annurev.pharmtox.40.1.431
63 schema:datePublished 2003-06
64 schema:datePublishedReg 2003-06-01
65 schema:description The nicotinic acetylcholine receptor controls electrical signalling between nerve and muscle cells by opening and closing a gated, membrane-spanning pore. Here we present an atomic model of the closed pore, obtained by electron microscopy of crystalline postsynaptic membranes. The pore is shaped by an inner ring of 5 alpha-helices, which curve radially to create a tapering path for the ions, and an outer ring of 15 alpha-helices, which coil around each other and shield the inner ring from the lipids. The gate is a constricting hydrophobic girdle at the middle of the lipid bilayer, formed by weak interactions between neighbouring inner helices. When acetylcholine enters the ligand-binding domain, it triggers rotations of the protein chains on opposite sides of the entrance to the pore. These rotations are communicated through the inner helices, and open the pore by breaking the girdle apart.
66 schema:genre research_article
67 schema:inLanguage en
68 schema:isAccessibleForFree false
69 schema:isPartOf N607eef2322f343598d0c96657ca5c970
70 Na6be4af1250b44f381e0ccd710c73f68
71 sg:journal.1018957
72 schema:name Structure and gating mechanism of the acetylcholine receptor pore
73 schema:pagination 949
74 schema:productId N1f5342e43ebf47fbb9e1ccae2134844c
75 N7551a9cbca2546489f60be4399ca6b04
76 Nc87c6986edf94a34bb43c5cb03dcfd73
77 Nd7c66d6d6ddb4de6bcdf505866258455
78 Nde83e02c116244cfa60dfb81beadf273
79 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010350589
80 https://doi.org/10.1038/nature01748
81 schema:sdDatePublished 2019-04-11T12:26
82 schema:sdLicense https://scigraph.springernature.com/explorer/license/
83 schema:sdPublisher Nf8e6cc6e49d74400afed200aa30ee72f
84 schema:url https://www.nature.com/articles/nature01748
85 sgo:license sg:explorer/license/
86 sgo:sdDataset articles
87 rdf:type schema:ScholarlyArticle
88 N14e56e82797940849ba719471a42b4b8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Protein Folding
90 rdf:type schema:DefinedTerm
91 N1f5342e43ebf47fbb9e1ccae2134844c schema:name doi
92 schema:value 10.1038/nature01748
93 rdf:type schema:PropertyValue
94 N2872f4fe0d984cccb8e6b2f0ce633471 rdf:first sg:person.01265767156.09
95 rdf:rest Ncc70fc02d0204f63b5ab74ca5242d127
96 N5bee7ad76c0c495198aa4fef5223e60c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Torpedo
98 rdf:type schema:DefinedTerm
99 N607eef2322f343598d0c96657ca5c970 schema:issueNumber 6943
100 rdf:type schema:PublicationIssue
101 N7551a9cbca2546489f60be4399ca6b04 schema:name nlm_unique_id
102 schema:value 0410462
103 rdf:type schema:PropertyValue
104 N7bdce08e76654dc6b3839f96012ca2bc rdf:first sg:person.0611456533.48
105 rdf:rest rdf:nil
106 N8190017f037e45438773f80277a8ffba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Receptors, Cholinergic
108 rdf:type schema:DefinedTerm
109 N96dc0e0554844d9ca814dd99adb90cf6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Ion Channel Gating
111 rdf:type schema:DefinedTerm
112 N98dce6526ae74cfd995bffbec709fcbf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Crystallization
114 rdf:type schema:DefinedTerm
115 Na653428a252849cd9f0b3d5369a9392a schema:name *RIKEN Harima Institute, 1-1-1 Kouto, Mikazuki-cho, Sayo, Hyogo 679-5148, Japan
116 rdf:type schema:Organization
117 Na6be4af1250b44f381e0ccd710c73f68 schema:volumeNumber 423
118 rdf:type schema:PublicationVolume
119 Nb3adcdceb1f64010b28ba6b620d48f0b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Molecular Sequence Data
121 rdf:type schema:DefinedTerm
122 Nb9c4a216a0014d8a87cbfe34c2d3f6ab schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Models, Molecular
124 rdf:type schema:DefinedTerm
125 Nbcb0c4088bd14e09a1375f382dc6e912 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Acetylcholine
127 rdf:type schema:DefinedTerm
128 Nc0b06709b483473599f70d1adfe3774d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Electric Conductivity
130 rdf:type schema:DefinedTerm
131 Nc691f0ff2fb246f3a465a0d3f01d1654 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Animals
133 rdf:type schema:DefinedTerm
134 Nc87c6986edf94a34bb43c5cb03dcfd73 schema:name readcube_id
135 schema:value 314faa065ba16be2be94de2fd3656359973ca53fd1eb1496c8f9d2b96e869cde
136 rdf:type schema:PropertyValue
137 Ncc70fc02d0204f63b5ab74ca5242d127 rdf:first sg:person.01077633456.74
138 rdf:rest N7bdce08e76654dc6b3839f96012ca2bc
139 Ncd2e43743b3f437abd36849302fa96d6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Ions
141 rdf:type schema:DefinedTerm
142 Nd7c66d6d6ddb4de6bcdf505866258455 schema:name pubmed_id
143 schema:value 12827192
144 rdf:type schema:PropertyValue
145 Nde83e02c116244cfa60dfb81beadf273 schema:name dimensions_id
146 schema:value pub.1010350589
147 rdf:type schema:PropertyValue
148 Ne2cf0fc833f54b22b634f27cd02243db schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Protein Conformation
150 rdf:type schema:DefinedTerm
151 Neac5ea374b334a86900133a8c7ace537 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Amino Acid Sequence
153 rdf:type schema:DefinedTerm
154 Nf8e6cc6e49d74400afed200aa30ee72f schema:name Springer Nature - SN SciGraph project
155 rdf:type schema:Organization
156 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
157 schema:name Biological Sciences
158 rdf:type schema:DefinedTerm
159 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
160 schema:name Biochemistry and Cell Biology
161 rdf:type schema:DefinedTerm
162 sg:journal.1018957 schema:issn 0090-0028
163 1476-4687
164 schema:name Nature
165 rdf:type schema:Periodical
166 sg:person.01077633456.74 schema:affiliation https://www.grid.ac/institutes/grid.258799.8
167 schema:familyName Fujiyoshi
168 schema:givenName Yoshinori
169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01077633456.74
170 rdf:type schema:Person
171 sg:person.01265767156.09 schema:affiliation Na653428a252849cd9f0b3d5369a9392a
172 schema:familyName Miyazawa
173 schema:givenName Atsuo
174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01265767156.09
175 rdf:type schema:Person
176 sg:person.0611456533.48 schema:affiliation https://www.grid.ac/institutes/grid.42475.30
177 schema:familyName Unwin
178 schema:givenName Nigel
179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0611456533.48
180 rdf:type schema:Person
181 sg:pub.10.1038/324670a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041383088
182 https://doi.org/10.1038/324670a0
183 rdf:type schema:CreativeWork
184 sg:pub.10.1038/335645a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035437234
185 https://doi.org/10.1038/335645a0
186 rdf:type schema:CreativeWork
187 sg:pub.10.1038/336247a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004057678
188 https://doi.org/10.1038/336247a0
189 rdf:type schema:CreativeWork
190 sg:pub.10.1038/35077011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044023349
191 https://doi.org/10.1038/35077011
192 rdf:type schema:CreativeWork
193 sg:pub.10.1038/366479a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030007131
194 https://doi.org/10.1038/366479a0
195 rdf:type schema:CreativeWork
196 sg:pub.10.1038/373037a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050150805
197 https://doi.org/10.1038/373037a0
198 rdf:type schema:CreativeWork
199 sg:pub.10.1038/376514a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037506717
200 https://doi.org/10.1038/376514a0
201 rdf:type schema:CreativeWork
202 sg:pub.10.1038/386088a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050523440
203 https://doi.org/10.1038/386088a0
204 rdf:type schema:CreativeWork
205 sg:pub.10.1038/6326 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029776234
206 https://doi.org/10.1038/6326
207 rdf:type schema:CreativeWork
208 sg:pub.10.1038/nrn731 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042688388
209 https://doi.org/10.1038/nrn731
210 rdf:type schema:CreativeWork
211 https://app.dimensions.ai/details/publication/pub.1077171507 schema:CreativeWork
212 https://app.dimensions.ai/details/publication/pub.1082415541 schema:CreativeWork
213 https://doi.org/10.1002/jcc.540120405 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000521002
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1006/jmbi.1993.1107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042006239
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1006/jmbi.1999.2721 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041196714
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1016/0014-5793(86)80881-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1030637209
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1016/0022-2836(70)90036-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030728416
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1016/0263-7855(93)87009-t schema:sameAs https://app.dimensions.ai/details/publication/pub.1001021383
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1016/0304-3991(87)90010-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044613010
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1016/0304-3991(91)90159-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006639320
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1016/0304-3991(92)90003-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034114385
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1016/0896-6273(90)90445-l schema:sameAs https://app.dimensions.ai/details/publication/pub.1038680263
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1016/0896-6273(94)90257-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011410608
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1016/s0006-3495(93)81293-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004280208
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1016/s0022-2836(02)00381-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001869447
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1016/s0304-3991(97)00070-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029131989
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1016/s0304-3991(99)00120-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004596493
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1016/s0896-6273(00)80506-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036914579
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1021/bi00176a016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055163894
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1021/bi980143m schema:sameAs https://app.dimensions.ai/details/publication/pub.1055215483
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1021/jp012233y schema:sameAs https://app.dimensions.ai/details/publication/pub.1056046871
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1073/pnas.160128797 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017014142
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1073/pnas.83.8.2719 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019541501
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1073/pnas.92.3.758 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041527171
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1073/pnas.95.11.6504 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001965255
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1074/jbc.273.15.8659 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031451936
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1083/jcb.82.1.150 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042322385
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1083/jcb.99.4.1202 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022508867
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1085/jgp.116.3.327 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046349217
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1093/hmg/6.5.767 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002561885
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1098/rspb.1991.0012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037519871
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1107/s0021889891004399 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014885043
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1107/s0021889892009944 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003451988
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1107/s0108767390010224 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014210169
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1107/s0365110x58000517 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023091745
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1146/annurev.pharmtox.40.1.431 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035865674
280 rdf:type schema:CreativeWork
281 https://www.grid.ac/institutes/grid.258799.8 schema:alternateName Kyoto University
282 schema:name †Department of Biophysics, Faculty of Science, Kyoto University, Oiwake, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan
283 rdf:type schema:Organization
284 https://www.grid.ac/institutes/grid.42475.30 schema:alternateName MRC Laboratory of Molecular Biology
285 schema:name ‡MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
286 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...