X-ray structure of a voltage-dependent K+ channel View Full Text


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Article Info

DATE

2003-05

AUTHORS

Youxing Jiang, Alice Lee, Jiayun Chen, Vanessa Ruta, Martine Cadene, Brian T Chait, Roderick MacKinnon

ABSTRACT

Voltage-dependent K+ channels are members of the family of voltage-dependent cation (K+, Na+ and Ca2+) channels that open and allow ion conduction in response to changes in cell membrane voltage. This form of gating underlies the generation of nerve and muscle action potentials, among other processes. Here we present the structure of KvAP, a voltage-dependent K+ channel from Aeropyrum pernix. We have determined a crystal structure of the full-length channel at a resolution of 3.2 A, and of the isolated voltage-sensor domain at 1.9 A, both in complex with monoclonal Fab fragments. The channel contains a central ion-conduction pore surrounded by voltage sensors, which form what we call 'voltage-sensor paddles'-hydrophobic, cationic, helix-turn-helix structures on the channel's outer perimeter. Flexible hinges suggest that the voltage-sensor paddles move in response to membrane voltage changes, carrying their positive charge across the membrane. More... »

PAGES

33

Journal

TITLE

Nature

ISSUE

6935

VOLUME

423

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nature01580

    DOI

    http://dx.doi.org/10.1038/nature01580

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1046608420

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/12721618


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    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature01580'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature01580'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature01580'


     

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