Protein analysis on a proteomic scale View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-03

AUTHORS

Eric Phizicky, Philippe I. H. Bastiaens, Heng Zhu, Michael Snyder, Stanley Fields

ABSTRACT

The long-term challenge of proteomics is enormous: to define the identities, quantities, structures and functions of complete complements of proteins, and to characterize how these properties vary in different cellular contexts. One critical step in tackling this goal is the generation of sets of clones that express a representative of each protein of a proteome in a useful format, followed by the analysis of these sets on a genome-wide basis. Such studies enable genetic, biochemical and cell biological technologies to be applied on a systematic level, leading to the assignment of biochemical activities, the construction of protein arrays, the identification of interactions, and the localization of proteins within cellular compartments. More... »

PAGES

208-215

References to SciGraph publications

  • 2001-01. The protein–protein interaction map of Helicobacter pylori in NATURE
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  • 2000-11. Analysis of yeast protein kinases using protein chips in NATURE GENETICS
  • 1989-07. A novel genetic system to detect protein–protein interactions in NATURE
  • 2002-01. Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry in NATURE
  • 2002-01. Functional organization of the yeast proteome by systematic analysis of protein complexes in NATURE
  • 2001-03. Open-reading-frame sequence tags (OSTs) support the existence of at least 17,300 genes in C. elegans in NATURE GENETICS
  • 2001-01-22. Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions in GENOME BIOLOGY
  • 1999-10. A generic protein purification method for protein complex characterization and proteome exploration in NATURE BIOTECHNOLOGY
  • 2002-07-01. Proteomic profiling of mechanistically distinct enzyme classes using a common chemotype in NATURE BIOTECHNOLOGY
  • 2002-03. Carbohydrate microarrays for the recognition of cross-reactive molecular markers of microbes and host cells in NATURE BIOTECHNOLOGY
  • 2002-03. Peptide chips for the quantitative evaluation of protein kinase activity in NATURE BIOTECHNOLOGY
  • 2000-09. A chemical switch for inhibitor-sensitive alleles of any protein kinase in NATURE
  • 1998-06. Bcl-2 and Bax interactions in mitochondria probed with green fluorescent protein and fluorescence resonance energy transfer in NATURE BIOTECHNOLOGY
  • 2002-01-01. An integrated approach for finding overlooked genes in yeast in NATURE BIOTECHNOLOGY
  • 2000-02. A comprehensive analysis of protein–protein interactions in Saccharomyces cerevisiae in NATURE
  • 2001-05-03. Microarrays of cells expressing defined cDNAs in NATURE
  • 2002-05-08. Comparative assessment of large-scale data sets of protein–protein interactions in NATURE
  • 2002-04. Dissecting glucose signalling with diversity-oriented synthesis and small-molecule microarrays in NATURE
  • Journal

    TITLE

    Nature

    ISSUE

    6928

    VOLUME

    422

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/nature01512

    DOI

    http://dx.doi.org/10.1038/nature01512

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1052020554

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/12634794


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