A molecular programme for the specification of germ cell fate in mice View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2002-07

AUTHORS

Mitinori Saitou, Sheila C. Barton, M. Azim Surani

ABSTRACT

Germ cell fate in mice is induced in proximal epiblast cells by the extra-embryonic ectoderm, and is not acquired through the inheritance of any preformed germ plasm. To determine precisely how germ cells are specified, we performed a genetic screen between single nascent germ cells and their somatic neighbours that share common ancestry. Here we show that fragilis, an interferon-inducible transmembrane protein, marks the onset of germ cell competence, and we propose that through homotypic association, it demarcates germ cells from somatic neighbours. Using single-cell gene expression profiles, we also show that only those cells with the highest expression of fragilis subsequently express stella, a gene that we detected exclusively in lineage-restricted germ cells. The stella positive nascent germ cells exhibit repression of homeobox genes, which may explain their escape from a somatic cell fate and the retention of pluripotency. More... »

PAGES

293

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/nature00927

DOI

http://dx.doi.org/10.1038/nature00927

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1012360268

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12124616


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bone Morphogenetic Protein 4", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bone Morphogenetic Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Differentiation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Lineage", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Down-Regulation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ectoderm", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Embryonic Induction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Deletion", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Profiling", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation, Developmental", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Homeobox", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Germ Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immunohistochemistry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "In Situ Hybridization", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Membrane Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Knockout", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Messenger", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Repressor Proteins", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Cambridge", 
          "id": "https://www.grid.ac/institutes/grid.5335.0", 
          "name": [
            "*Wellcome Trust/Cancer Research UK Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Saitou", 
        "givenName": "Mitinori", 
        "id": "sg:person.01265450666.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01265450666.34"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Cambridge", 
          "id": "https://www.grid.ac/institutes/grid.5335.0", 
          "name": [
            "*Wellcome Trust/Cancer Research UK Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Barton", 
        "givenName": "Sheila C.", 
        "id": "sg:person.01123202640.92", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123202640.92"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Cambridge", 
          "id": "https://www.grid.ac/institutes/grid.5335.0", 
          "name": [
            "*Wellcome Trust/Cancer Research UK Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Surani", 
        "givenName": "M. Azim", 
        "id": "sg:person.01121607454.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01121607454.03"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/0076-6879(93)25025-w", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001449256"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)81783-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002001169"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/aja.1001990108", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002682423"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.9.17.2105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003744681"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0959-437x(93)90104-w", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005634383"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0925-4773(01)00499-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006735136"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.13.4.373", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010083594"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)81769-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011032363"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(95)90161-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012376657"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0925-4773(02)00002-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013502294"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(84)90270-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013986393"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0925-4773(00)00283-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014417740"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.13.4.424", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015917256"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.86.3.840", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016087114"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/9780470514573.ch5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022227207"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0076-6879(93)25039-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022632583"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1521-1878(199809)20:9<722::aid-bies5>3.0.co;2-i", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026758842"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/ar.1091180202", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027852611"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/dbio.1996.0203", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030577115"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/dbio.1994.1018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031048582"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.141.2.397", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032902235"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-0436.1984.tb01433.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034511835"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/dbio.2001.0264", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041819101"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.151242798", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042298650"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.91.25.12258", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042614837"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)80557-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043267749"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/343617a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044546418", 
          "https://doi.org/10.1038/343617a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0968-0004(99)01537-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045459908"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/375787a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048699343", 
          "https://doi.org/10.1038/375787a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0074-7696(08)60070-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050651915"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/dbio.2001.0173", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050687955"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.270.40.23860", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051702623"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1210/mend.14.7.0479", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1064332014"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074486514", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074752669", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077596060", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1990.tb08309.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077950704"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077971973", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078351467", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078571188", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082404858", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082499260", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082508422", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082659680", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082731181", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082800772", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082886299", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2002-07", 
    "datePublishedReg": "2002-07-01", 
    "description": "Germ cell fate in mice is induced in proximal epiblast cells by the extra-embryonic ectoderm, and is not acquired through the inheritance of any preformed germ plasm. To determine precisely how germ cells are specified, we performed a genetic screen between single nascent germ cells and their somatic neighbours that share common ancestry. Here we show that fragilis, an interferon-inducible transmembrane protein, marks the onset of germ cell competence, and we propose that through homotypic association, it demarcates germ cells from somatic neighbours. Using single-cell gene expression profiles, we also show that only those cells with the highest expression of fragilis subsequently express stella, a gene that we detected exclusively in lineage-restricted germ cells. The stella positive nascent germ cells exhibit repression of homeobox genes, which may explain their escape from a somatic cell fate and the retention of pluripotency.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/nature00927", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6895", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "418"
      }
    ], 
    "name": "A molecular programme for the specification of germ cell fate in mice", 
    "pagination": "293", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "3ae375ce6defa7045d0f431f646aabca58fe808dc67490220218080d733fbeb3"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "12124616"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/nature00927"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1012360268"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/nature00927", 
      "https://app.dimensions.ai/details/publication/pub.1012360268"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:15", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000361_0000000361/records_54014_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/nature00927"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/nature00927'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/nature00927'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/nature00927'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/nature00927'


 

This table displays all metadata directly associated to this object as RDF triples.

297 TRIPLES      21 PREDICATES      97 URIs      42 LITERALS      30 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/nature00927 schema:about N19952db982814c6185d110ce2fc4c237
2 N1af1944568684a38ab8ee73a19c57e04
3 N2ba4349b3d704d588a13281667c1e7d1
4 N2fbebbd5afbe4a1aa0ef7cbe58a970d2
5 N3713b0b3ac7b4b0b8bb30a0c6cc72215
6 N427d7dedcffb4b399810def735bf62ec
7 N504757380e3b44d2aec5357e038bea4d
8 N5616a91d89704f45bd095315b0ec4ddd
9 N5ce755665f6c4106a49855291060a8f5
10 N635c5df8e0a449a28d5b372e1d0442d3
11 N63f13d0334344614934afb43790c43ff
12 N7ccf40878cdc4f279c34906937bbde50
13 N854567c311cb4fe098f0ca4f46a1b735
14 N8a3d456c30574ad494f3426e7445b073
15 N9314ce0e2e484382b2d632bae640cfd3
16 N9745c37daf114a42b7024eb23ced3c18
17 Na00574972a9946d5a5767ce580555e0b
18 Naaaff86ede47493ca3a7d7904b1ac20f
19 Nbd947539792a423ab2fe9923da4bbdf8
20 Ndd7901ebad4b498d838930a72e4dd7dd
21 Ne82e06b454a0474f8f19d5126b72e56b
22 anzsrc-for:06
23 anzsrc-for:0604
24 schema:author Nadd863975a6a423badd948976c94da66
25 schema:citation sg:pub.10.1038/343617a0
26 sg:pub.10.1038/375787a0
27 https://app.dimensions.ai/details/publication/pub.1074486514
28 https://app.dimensions.ai/details/publication/pub.1074752669
29 https://app.dimensions.ai/details/publication/pub.1077596060
30 https://app.dimensions.ai/details/publication/pub.1077971973
31 https://app.dimensions.ai/details/publication/pub.1078351467
32 https://app.dimensions.ai/details/publication/pub.1078571188
33 https://app.dimensions.ai/details/publication/pub.1082404858
34 https://app.dimensions.ai/details/publication/pub.1082499260
35 https://app.dimensions.ai/details/publication/pub.1082508422
36 https://app.dimensions.ai/details/publication/pub.1082659680
37 https://app.dimensions.ai/details/publication/pub.1082731181
38 https://app.dimensions.ai/details/publication/pub.1082800772
39 https://app.dimensions.ai/details/publication/pub.1082886299
40 https://doi.org/10.1002/(sici)1521-1878(199809)20:9<722::aid-bies5>3.0.co;2-i
41 https://doi.org/10.1002/9780470514573.ch5
42 https://doi.org/10.1002/aja.1001990108
43 https://doi.org/10.1002/ar.1091180202
44 https://doi.org/10.1002/j.1460-2075.1990.tb08309.x
45 https://doi.org/10.1006/dbio.1994.1018
46 https://doi.org/10.1006/dbio.1996.0203
47 https://doi.org/10.1006/dbio.2001.0173
48 https://doi.org/10.1006/dbio.2001.0264
49 https://doi.org/10.1016/0076-6879(93)25025-w
50 https://doi.org/10.1016/0076-6879(93)25039-5
51 https://doi.org/10.1016/0092-8674(84)90270-8
52 https://doi.org/10.1016/0092-8674(95)90161-2
53 https://doi.org/10.1016/0959-437x(93)90104-w
54 https://doi.org/10.1016/s0074-7696(08)60070-4
55 https://doi.org/10.1016/s0092-8674(00)80557-7
56 https://doi.org/10.1016/s0092-8674(00)81769-9
57 https://doi.org/10.1016/s0092-8674(00)81783-3
58 https://doi.org/10.1016/s0925-4773(00)00283-5
59 https://doi.org/10.1016/s0925-4773(01)00499-3
60 https://doi.org/10.1016/s0925-4773(02)00002-3
61 https://doi.org/10.1016/s0968-0004(99)01537-6
62 https://doi.org/10.1073/pnas.151242798
63 https://doi.org/10.1073/pnas.86.3.840
64 https://doi.org/10.1073/pnas.91.25.12258
65 https://doi.org/10.1074/jbc.270.40.23860
66 https://doi.org/10.1083/jcb.141.2.397
67 https://doi.org/10.1101/gad.13.4.373
68 https://doi.org/10.1101/gad.13.4.424
69 https://doi.org/10.1101/gad.9.17.2105
70 https://doi.org/10.1111/j.1432-0436.1984.tb01433.x
71 https://doi.org/10.1210/mend.14.7.0479
72 schema:datePublished 2002-07
73 schema:datePublishedReg 2002-07-01
74 schema:description Germ cell fate in mice is induced in proximal epiblast cells by the extra-embryonic ectoderm, and is not acquired through the inheritance of any preformed germ plasm. To determine precisely how germ cells are specified, we performed a genetic screen between single nascent germ cells and their somatic neighbours that share common ancestry. Here we show that fragilis, an interferon-inducible transmembrane protein, marks the onset of germ cell competence, and we propose that through homotypic association, it demarcates germ cells from somatic neighbours. Using single-cell gene expression profiles, we also show that only those cells with the highest expression of fragilis subsequently express stella, a gene that we detected exclusively in lineage-restricted germ cells. The stella positive nascent germ cells exhibit repression of homeobox genes, which may explain their escape from a somatic cell fate and the retention of pluripotency.
75 schema:genre research_article
76 schema:inLanguage en
77 schema:isAccessibleForFree false
78 schema:isPartOf Nc87fc83590754779a3866cfbdc6db661
79 Nce9a5b0d3a3a464cb33b2fa4a48000e2
80 sg:journal.1018957
81 schema:name A molecular programme for the specification of germ cell fate in mice
82 schema:pagination 293
83 schema:productId N021bf8620506463fa0564431c6299666
84 N8aae8dfbe0bb43658101b50627af5c59
85 Ncc27eaa2ee1b45209c346d6b658a4321
86 Nd78d61c11daf4f6abff698ba0c21c93b
87 Nd8716e5c143b40739812b0d066909da6
88 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012360268
89 https://doi.org/10.1038/nature00927
90 schema:sdDatePublished 2019-04-11T12:15
91 schema:sdLicense https://scigraph.springernature.com/explorer/license/
92 schema:sdPublisher N97d5873a04b54e5289a03ac4d56015ab
93 schema:url https://www.nature.com/articles/nature00927
94 sgo:license sg:explorer/license/
95 sgo:sdDataset articles
96 rdf:type schema:ScholarlyArticle
97 N021bf8620506463fa0564431c6299666 schema:name readcube_id
98 schema:value 3ae375ce6defa7045d0f431f646aabca58fe808dc67490220218080d733fbeb3
99 rdf:type schema:PropertyValue
100 N126021cd7ebf458d8bcc947265ae5fe4 rdf:first sg:person.01121607454.03
101 rdf:rest rdf:nil
102 N19952db982814c6185d110ce2fc4c237 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name In Situ Hybridization
104 rdf:type schema:DefinedTerm
105 N1af1944568684a38ab8ee73a19c57e04 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Membrane Proteins
107 rdf:type schema:DefinedTerm
108 N2ba4349b3d704d588a13281667c1e7d1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Female
110 rdf:type schema:DefinedTerm
111 N2fbebbd5afbe4a1aa0ef7cbe58a970d2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Mice, Knockout
113 rdf:type schema:DefinedTerm
114 N3713b0b3ac7b4b0b8bb30a0c6cc72215 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Embryonic Induction
116 rdf:type schema:DefinedTerm
117 N427d7dedcffb4b399810def735bf62ec schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Down-Regulation
119 rdf:type schema:DefinedTerm
120 N504757380e3b44d2aec5357e038bea4d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Gene Expression Regulation, Developmental
122 rdf:type schema:DefinedTerm
123 N5616a91d89704f45bd095315b0ec4ddd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Cell Differentiation
125 rdf:type schema:DefinedTerm
126 N5ce755665f6c4106a49855291060a8f5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Repressor Proteins
128 rdf:type schema:DefinedTerm
129 N635c5df8e0a449a28d5b372e1d0442d3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name RNA, Messenger
131 rdf:type schema:DefinedTerm
132 N63f13d0334344614934afb43790c43ff schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Mice
134 rdf:type schema:DefinedTerm
135 N7ccf40878cdc4f279c34906937bbde50 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Ectoderm
137 rdf:type schema:DefinedTerm
138 N854567c311cb4fe098f0ca4f46a1b735 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Animals
140 rdf:type schema:DefinedTerm
141 N8a3d456c30574ad494f3426e7445b073 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Genes, Homeobox
143 rdf:type schema:DefinedTerm
144 N8aae8dfbe0bb43658101b50627af5c59 schema:name pubmed_id
145 schema:value 12124616
146 rdf:type schema:PropertyValue
147 N9314ce0e2e484382b2d632bae640cfd3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Immunohistochemistry
149 rdf:type schema:DefinedTerm
150 N9745c37daf114a42b7024eb23ced3c18 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Gene Deletion
152 rdf:type schema:DefinedTerm
153 N97d5873a04b54e5289a03ac4d56015ab schema:name Springer Nature - SN SciGraph project
154 rdf:type schema:Organization
155 Na00574972a9946d5a5767ce580555e0b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
156 schema:name Gene Expression Profiling
157 rdf:type schema:DefinedTerm
158 Naaaff86ede47493ca3a7d7904b1ac20f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
159 schema:name Bone Morphogenetic Protein 4
160 rdf:type schema:DefinedTerm
161 Nadd863975a6a423badd948976c94da66 rdf:first sg:person.01265450666.34
162 rdf:rest Nb6e5c87e7ba9464a87e52be90273545f
163 Nb6e5c87e7ba9464a87e52be90273545f rdf:first sg:person.01123202640.92
164 rdf:rest N126021cd7ebf458d8bcc947265ae5fe4
165 Nbd947539792a423ab2fe9923da4bbdf8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name Cell Lineage
167 rdf:type schema:DefinedTerm
168 Nc87fc83590754779a3866cfbdc6db661 schema:volumeNumber 418
169 rdf:type schema:PublicationVolume
170 Ncc27eaa2ee1b45209c346d6b658a4321 schema:name nlm_unique_id
171 schema:value 0410462
172 rdf:type schema:PropertyValue
173 Nce9a5b0d3a3a464cb33b2fa4a48000e2 schema:issueNumber 6895
174 rdf:type schema:PublicationIssue
175 Nd78d61c11daf4f6abff698ba0c21c93b schema:name doi
176 schema:value 10.1038/nature00927
177 rdf:type schema:PropertyValue
178 Nd8716e5c143b40739812b0d066909da6 schema:name dimensions_id
179 schema:value pub.1012360268
180 rdf:type schema:PropertyValue
181 Ndd7901ebad4b498d838930a72e4dd7dd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
182 schema:name Germ Cells
183 rdf:type schema:DefinedTerm
184 Ne82e06b454a0474f8f19d5126b72e56b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
185 schema:name Bone Morphogenetic Proteins
186 rdf:type schema:DefinedTerm
187 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
188 schema:name Biological Sciences
189 rdf:type schema:DefinedTerm
190 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
191 schema:name Genetics
192 rdf:type schema:DefinedTerm
193 sg:journal.1018957 schema:issn 0090-0028
194 1476-4687
195 schema:name Nature
196 rdf:type schema:Periodical
197 sg:person.01121607454.03 schema:affiliation https://www.grid.ac/institutes/grid.5335.0
198 schema:familyName Surani
199 schema:givenName M. Azim
200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01121607454.03
201 rdf:type schema:Person
202 sg:person.01123202640.92 schema:affiliation https://www.grid.ac/institutes/grid.5335.0
203 schema:familyName Barton
204 schema:givenName Sheila C.
205 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01123202640.92
206 rdf:type schema:Person
207 sg:person.01265450666.34 schema:affiliation https://www.grid.ac/institutes/grid.5335.0
208 schema:familyName Saitou
209 schema:givenName Mitinori
210 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01265450666.34
211 rdf:type schema:Person
212 sg:pub.10.1038/343617a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044546418
213 https://doi.org/10.1038/343617a0
214 rdf:type schema:CreativeWork
215 sg:pub.10.1038/375787a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048699343
216 https://doi.org/10.1038/375787a0
217 rdf:type schema:CreativeWork
218 https://app.dimensions.ai/details/publication/pub.1074486514 schema:CreativeWork
219 https://app.dimensions.ai/details/publication/pub.1074752669 schema:CreativeWork
220 https://app.dimensions.ai/details/publication/pub.1077596060 schema:CreativeWork
221 https://app.dimensions.ai/details/publication/pub.1077971973 schema:CreativeWork
222 https://app.dimensions.ai/details/publication/pub.1078351467 schema:CreativeWork
223 https://app.dimensions.ai/details/publication/pub.1078571188 schema:CreativeWork
224 https://app.dimensions.ai/details/publication/pub.1082404858 schema:CreativeWork
225 https://app.dimensions.ai/details/publication/pub.1082499260 schema:CreativeWork
226 https://app.dimensions.ai/details/publication/pub.1082508422 schema:CreativeWork
227 https://app.dimensions.ai/details/publication/pub.1082659680 schema:CreativeWork
228 https://app.dimensions.ai/details/publication/pub.1082731181 schema:CreativeWork
229 https://app.dimensions.ai/details/publication/pub.1082800772 schema:CreativeWork
230 https://app.dimensions.ai/details/publication/pub.1082886299 schema:CreativeWork
231 https://doi.org/10.1002/(sici)1521-1878(199809)20:9<722::aid-bies5>3.0.co;2-i schema:sameAs https://app.dimensions.ai/details/publication/pub.1026758842
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1002/9780470514573.ch5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022227207
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1002/aja.1001990108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002682423
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1002/ar.1091180202 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027852611
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1002/j.1460-2075.1990.tb08309.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077950704
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1006/dbio.1994.1018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031048582
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1006/dbio.1996.0203 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030577115
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1006/dbio.2001.0173 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050687955
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1006/dbio.2001.0264 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041819101
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1016/0076-6879(93)25025-w schema:sameAs https://app.dimensions.ai/details/publication/pub.1001449256
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1016/0076-6879(93)25039-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022632583
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1016/0092-8674(84)90270-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013986393
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1016/0092-8674(95)90161-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012376657
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1016/0959-437x(93)90104-w schema:sameAs https://app.dimensions.ai/details/publication/pub.1005634383
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1016/s0074-7696(08)60070-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050651915
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1016/s0092-8674(00)80557-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043267749
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1016/s0092-8674(00)81769-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011032363
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1016/s0092-8674(00)81783-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002001169
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1016/s0925-4773(00)00283-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014417740
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1016/s0925-4773(01)00499-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006735136
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1016/s0925-4773(02)00002-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013502294
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1016/s0968-0004(99)01537-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045459908
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1073/pnas.151242798 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042298650
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1073/pnas.86.3.840 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016087114
278 rdf:type schema:CreativeWork
279 https://doi.org/10.1073/pnas.91.25.12258 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042614837
280 rdf:type schema:CreativeWork
281 https://doi.org/10.1074/jbc.270.40.23860 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051702623
282 rdf:type schema:CreativeWork
283 https://doi.org/10.1083/jcb.141.2.397 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032902235
284 rdf:type schema:CreativeWork
285 https://doi.org/10.1101/gad.13.4.373 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010083594
286 rdf:type schema:CreativeWork
287 https://doi.org/10.1101/gad.13.4.424 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015917256
288 rdf:type schema:CreativeWork
289 https://doi.org/10.1101/gad.9.17.2105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003744681
290 rdf:type schema:CreativeWork
291 https://doi.org/10.1111/j.1432-0436.1984.tb01433.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1034511835
292 rdf:type schema:CreativeWork
293 https://doi.org/10.1210/mend.14.7.0479 schema:sameAs https://app.dimensions.ai/details/publication/pub.1064332014
294 rdf:type schema:CreativeWork
295 https://www.grid.ac/institutes/grid.5335.0 schema:alternateName University of Cambridge
296 schema:name *Wellcome Trust/Cancer Research UK Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, UK
297 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...