Calretinin immunohistochemistry: a simple and efficient tool to diagnose Hirschsprung disease View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2009-10

AUTHORS

Vincent Guinard-Samuel, Arnaud Bonnard, Pascal De Lagausie, Pascale Philippe-Chomette, Corine Alberti, Alaa El Ghoneimi, Michel Peuchmaur, Dominique Berrebi-Binczak

ABSTRACT

Diagnosis of Hirschsprung disease (HD) is quite entirely based on the histopathological analysis of suction rectal biopsies. This hematoxylin and eosin approach has some limitations, despite the help of acetylcholinesterase staining. The aim of this study was to assess the diagnostic value of calretinin immunochemistry as a simple and reliable method in the diagnosis of HD. A total of 131 initial rectal biopsies carried out for suspicion of HD in children were retrieved, and calretinin immunohistochemistry was carried out on paraffin-embedded biopsies. Diagnosis of HD was made when no staining was observed. The results were statistically analyzed in comparison with our standard method (histology and acetylcholinesterase staining). 130 biopsies were accurately diagnosed on the basis of the positivity or negativity of calretinin staining. The senior pathologists diagnosed all cases of HD with no false positives. Furthermore, 12 additional cases initially considered as doubtful for HD using the standard method, were accurately diagnosed using calretinin immunohistochemistry. The false negative was a case of HD with a calretinin-positive biopsy. We also demonstrate the ease of calretinin interpretation compared with acetylcholinesterase for the junior pathologist. Calretinin immunohistochemistry overcomes most of the difficulties encountered using the combination of histology and acetylcholinesterase staining, and detects almost all cases of HD with confidence, with no false positives. Thus, we demonstrate that calretinin is superior to acetylcholinesterase to complete histology and could advantageously substitute for acetylcholinesterase. More... »

PAGES

modpathol2009110

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/modpathol.2009.110

DOI

http://dx.doi.org/10.1038/modpathol.2009.110

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034559684

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19648883


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