Characterization of a novel anti-fatty acid synthase (FASN) antiserum in breast tissue View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2008-12

AUTHORS

Kristin C Jensen, David F Schaeffer, Maggie Cheang, Kelli Montgomery, Robert B West, C Blake Gilks, Doug Ross, Gulisa Turashvili, Stuart Schnitt, Matt van de Rijn

ABSTRACT

Fatty acid synthase (FASN) expression has been reported in many different tumors, including breast cancer. In gene microarray studies, the fatty acid synthase gene co-clustered with cytokeratins 5 and 17 and other genes that defined the basal-like subset of breast cancers. To define the use of this marker in breast pathology, a rabbit polyclonal antiserum (S143) to a peptide fragment of this gene was produced and compared with a commercially available monoclonal antibody by immunohistochemistry on various tissue microarrays and whole tissue sections. The tissue microarrays included 1090 breast cancers and 244 normal breast tissues. Whole tissue sections consisted of benign and malignant tissues from breast resection specimens. In contrast to other 'basal' markers identified by gene expression profiling data, the fatty acid synthase (FASN) expression pattern in normal breast was notable for its expression in only a small subset of basal and suprabasal cells. Dual staining experiments revealed that the subpopulation of cells labeling with FASN did not coexpress myoepithelial markers CK5/6 or p63, but did coexpress e-cadherin. In addition to staining a subset of basal and suprabasal cells, the antiserum highlighted apocrine differentiation, and stained 106/144 (74%) cases of columnar cell lesions and five of five cases of flat epithelial atypia. Despite its association with basal keratins in gene array studies, FASN expression did not correlate significantly with the outcome in breast cancer. We describe an expression pattern that highlights only a subset of basal and suprabasal cells in normal breast ducts and we show by dual expression studies that this subset of cells is different from myoepithelial and basal cytokeratin-positive cells. In addition, FASN expression is described in apocrine metaplasia, columnar cell lesions, and flat epithelial atypia. More... »

PAGES

modpathol2008163

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/modpathol.2008.163

DOI

http://dx.doi.org/10.1038/modpathol.2008.163

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031771594

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18820672


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