Podocyte Injury Promotes Progressive Nephropathy in Zucker Diabetic Fatty Rats View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2002-01-01

AUTHORS

Sachi Hoshi, Yujing Shu, Fusayo Yoshida, Tomoko Inagaki, Jiro Sonoda, Teruo Watanabe, Ken-ichi Nomoto, Michio Nagata

ABSTRACT

The zucker diabetic fatty (ZDF-fa/fa) rat is one of the attractive models for type II diabetes based on impaired glucose tolerance caused by the inherited insulin-resistance gene fa. Characterization of nephropathy in this model may provide useful insights into the mechanism of the progression of diabetic nephropathy. The present study analyzed the pathophysiology of diabetes and nephropathy, including the process of glomerulosclerosis in this model by biochemical and morphometric analyses. In addition, we conducted studies in podocytes in culture to examine the direct effects of high glucose on podocytes. ZDF-fa/fa rats showed overt diabetes despite hyperinsulinemia as early as 3 months of age. Blood glucose levels increased further with a considerable decrease of insulin levels at 5 months. Glomerular filtration rate (GFR) was significantly elevated until 3 months, but fell to the level seen in lean rats by 7 months. Proteinuria started to rise during the period of increased GFR, and increased further after GFR had fallen to within the normal range. Renal fibronectin, collagen iv, and vascular endothelial growth factor mRNA levels were increased at 7 months. Glomerulosclerosis commenced as early as 5 months of age, and was associated with glomerular hypertrophy and mild mesangial expansion with evidence of accentuated podocyte injury, as revealed by increased expression of desmin. Electron microscopy suggested that degeneration of podocytes and the development of tuft adhesions were responsible for the glomerular sclerosis in this model. In addition, glomeruli from the diabetic rats showed up-regulation of the cyclin kinase inhibitors, p21 and p27. Further studies suggested that the increase in p27 expression was predominantly caused by podocytes, because predominant immunolocalization of p27 in podocytes in diabetic rats and high glucose medium induced cell hypertrophy accompanied by p27 up-regulation in differentiated podocyte cell lines. In conclusion, progressive diabetic nephropathy in ZDF-fa/fa rats is associated with evidence of podocyte injury. High concentrations of ambient glucose induced podocyte hypertrophy and stress in vitro, suggesting that the podocyte is a likely target of the diabetic milieu. More... »

PAGES

25-35

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/labinvest.3780392

DOI

http://dx.doi.org/10.1038/labinvest.3780392

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1008745799

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11796823


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Aging", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Blood Glucose", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Collagen Type IV", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Diabetes Mellitus, Type 2", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Diabetic Nephropathies", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Disease Models, Animal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Disease Progression", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Endothelial Growth Factors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Epithelial Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fibronectins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glomerular Filtration Rate", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "In Vitro Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Insulin Resistance", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kidney", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lymphokines", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rats", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rats, Zucker", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transcription, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Vascular Endothelial Growth Factor A", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Vascular Endothelial Growth Factors", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.20515.33", 
          "name": [
            "Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hoshi", 
        "givenName": "Sachi", 
        "id": "sg:person.01100676737.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01100676737.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.20515.33", 
          "name": [
            "Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shu", 
        "givenName": "Yujing", 
        "id": "sg:person.0615210032.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0615210032.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Cardiovascular Research, Eisai Research Laboratories, Tsukuba, Japan", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Department of Cardiovascular Research, Eisai Research Laboratories, Tsukuba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yoshida", 
        "givenName": "Fusayo", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pathology, Showa University, Tokyo, Japan", 
          "id": "http://www.grid.ac/institutes/grid.410714.7", 
          "name": [
            "Department of Pathology, Showa University, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Inagaki", 
        "givenName": "Tomoko", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Cardiovascular Research, Eisai Research Laboratories, Tsukuba, Japan", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Department of Cardiovascular Research, Eisai Research Laboratories, Tsukuba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sonoda", 
        "givenName": "Jiro", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.20515.33", 
          "name": [
            "Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Watanabe", 
        "givenName": "Teruo", 
        "id": "sg:person.012664624074.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012664624074.29"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Cardiovascular Research, Eisai Research Laboratories, Tsukuba, Japan", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Department of Cardiovascular Research, Eisai Research Laboratories, Tsukuba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nomoto", 
        "givenName": "Ken-ichi", 
        "id": "sg:person.01147012137.66", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01147012137.66"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Clinical Medicine, University of Tsukuba, Tsukuba, Japan", 
          "id": "http://www.grid.ac/institutes/grid.20515.33", 
          "name": [
            "Clinical Medicine, University of Tsukuba, Tsukuba, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nagata", 
        "givenName": "Michio", 
        "id": "sg:person.01022702772.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01022702772.51"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s004280050420", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039232099", 
          "https://doi.org/10.1007/s004280050420"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00429780", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028158860", 
          "https://doi.org/10.1007/bf00429780"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01222198", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010446785", 
          "https://doi.org/10.1007/bf01222198"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s001250051198", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042150322", 
          "https://doi.org/10.1007/s001250051198"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm0598-575", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043972437", 
          "https://doi.org/10.1038/nm0598-575"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2002-01-01", 
    "datePublishedReg": "2002-01-01", 
    "description": "The zucker diabetic fatty (ZDF-fa/fa) rat is one of the attractive models for type II diabetes based on impaired glucose tolerance caused by the inherited insulin-resistance gene fa. Characterization of nephropathy in this model may provide useful insights into the mechanism of the progression of diabetic nephropathy. The present study analyzed the pathophysiology of diabetes and nephropathy, including the process of glomerulosclerosis in this model by biochemical and morphometric analyses. In addition, we conducted studies in podocytes in culture to examine the direct effects of high glucose on podocytes. ZDF-fa/fa rats showed overt diabetes despite hyperinsulinemia as early as 3 months of age. Blood glucose levels increased further with a considerable decrease of insulin levels at 5 months. Glomerular filtration rate (GFR) was significantly elevated until 3 months, but fell to the level seen in lean rats by 7 months. Proteinuria started to rise during the period of increased GFR, and increased further after GFR had fallen to within the normal range. Renal fibronectin, collagen iv, and vascular endothelial growth factor mRNA levels were increased at 7 months. Glomerulosclerosis commenced as early as 5 months of age, and was associated with glomerular hypertrophy and mild mesangial expansion with evidence of accentuated podocyte injury, as revealed by increased expression of desmin. Electron microscopy suggested that degeneration of podocytes and the development of tuft adhesions were responsible for the glomerular sclerosis in this model. In addition, glomeruli from the diabetic rats showed up-regulation of the cyclin kinase inhibitors, p21 and p27. Further studies suggested that the increase in p27 expression was predominantly caused by podocytes, because predominant immunolocalization of p27 in podocytes in diabetic rats and high glucose medium induced cell hypertrophy accompanied by p27 up-regulation in differentiated podocyte cell lines. In conclusion, progressive diabetic nephropathy in ZDF-fa/fa rats is associated with evidence of podocyte injury. High concentrations of ambient glucose induced podocyte hypertrophy and stress in vitro, suggesting that the podocyte is a likely target of the diabetic milieu.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/labinvest.3780392", 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1017964", 
        "issn": [
          "0023-6837", 
          "1530-0307"
        ], 
        "name": "Laboratory Investigation", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "82"
      }
    ], 
    "keywords": [
      "glomerular filtration rate", 
      "ZDF fa/fa rats", 
      "Zucker diabetic fatty rats", 
      "diabetic fatty rats", 
      "months of age", 
      "diabetic nephropathy", 
      "diabetic rats", 
      "fatty rats", 
      "podocyte injury", 
      "fa rats", 
      "growth factor mRNA levels", 
      "mild mesangial expansion", 
      "progressive diabetic nephropathy", 
      "impaired glucose tolerance", 
      "process of glomerulosclerosis", 
      "blood glucose levels", 
      "vascular endothelial growth factor (VEGF) mRNA levels", 
      "pathophysiology of diabetes", 
      "factor mRNA levels", 
      "type II diabetes", 
      "high glucose medium", 
      "renal fibronectin", 
      "progressive nephropathy", 
      "glomerular hypertrophy", 
      "glucose tolerance", 
      "mesangial expansion", 
      "glomerular sclerosis", 
      "insulin levels", 
      "filtration rate", 
      "diabetic milieu", 
      "tuft adhesion", 
      "lean rats", 
      "glucose levels", 
      "nephropathy", 
      "normal range", 
      "ambient glucose", 
      "p27 expression", 
      "II diabetes", 
      "podocyte hypertrophy", 
      "high glucose", 
      "cell hypertrophy", 
      "cyclin kinase inhibitor", 
      "rats", 
      "increased expression", 
      "podocyte cell line", 
      "hypertrophy", 
      "kinase inhibitors", 
      "months", 
      "podocytes", 
      "mRNA levels", 
      "genes Fas", 
      "glomerulosclerosis", 
      "Further studies", 
      "diabetes", 
      "cell lines", 
      "collagen IV", 
      "p27", 
      "injury", 
      "present study", 
      "age", 
      "morphometric analysis", 
      "direct effect", 
      "likely target", 
      "glucose", 
      "proteinuria", 
      "hyperinsulinemia", 
      "sclerosis", 
      "pathophysiology", 
      "levels", 
      "expression", 
      "glomeruli", 
      "degeneration", 
      "progression", 
      "desmin", 
      "study", 
      "evidence", 
      "high concentrations", 
      "inhibitors", 
      "p21", 
      "regulation", 
      "attractive model", 
      "immunolocalization", 
      "glucose medium", 
      "conclusion", 
      "fibronectin", 
      "milieu", 
      "biochemical", 
      "FA", 
      "considerable decrease", 
      "target", 
      "period", 
      "addition", 
      "decrease", 
      "rate", 
      "increase", 
      "effect", 
      "tolerance", 
      "concentration", 
      "culture", 
      "mechanism", 
      "adhesion", 
      "stress", 
      "development", 
      "lines", 
      "model", 
      "analysis", 
      "insights", 
      "electron microscopy", 
      "medium", 
      "microscopy", 
      "expansion", 
      "characterization", 
      "range", 
      "useful insights", 
      "process"
    ], 
    "name": "Podocyte Injury Promotes Progressive Nephropathy in Zucker Diabetic Fatty Rats", 
    "pagination": "25-35", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1008745799"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/labinvest.3780392"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "11796823"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/labinvest.3780392", 
      "https://app.dimensions.ai/details/publication/pub.1008745799"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-08-04T16:55", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_360.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/labinvest.3780392"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/labinvest.3780392'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/labinvest.3780392'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/labinvest.3780392'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/labinvest.3780392'


 

This table displays all metadata directly associated to this object as RDF triples.

342 TRIPLES      21 PREDICATES      168 URIs      155 LITERALS      30 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/labinvest.3780392 schema:about N0225f85a24cb43bdbb8b36df375f8d23
2 N051c11ddab1c4053a9c672a58ac66c47
3 N052e225982ca4b9a9d75fd80b54d3b30
4 N079d5b0ed7f3412481165880f1a44504
5 N1630a57ecf554f0480f90b9e2ec57a7d
6 N29a8c0f52497466899c2276f53301095
7 N3d842f96962943fdaf92f1d8a5b6635b
8 N49d9c0c9f7c84bc0aa0cf0e07e17051c
9 N536f04a3fe2c4b7b937a94164d0bed32
10 N55a60ee0804548aea0b93370fb48bb2e
11 N55a8cb259dc8450cae3f2e9d13f2bdaf
12 N5d037b72dd5045e08da4696d704b2698
13 N63857fa224c2488dafcc69fbd17d20c0
14 N664004ddef4f48ed90899941134e41e2
15 N69b8da9f99b2404597be74f2c54e436b
16 N6de85a671fdf4b5380edb4db0e6452f4
17 N7f3f6668cc4f4756932bf0da118443cc
18 Na720a7020b804959b73d1ce6c438d9ae
19 Na81d51d5af974a6383153b7d9c42cb48
20 Nc03e37f782e34e71800040131a034cc9
21 Nc8b0724444ad47ab98634d9eb79d7eb5
22 Nd43bdc0fb88d45489d428bf8dcead7d8
23 Ne7aa4abbc7af42d89c7d12dbc4eaeccc
24 anzsrc-for:11
25 anzsrc-for:1103
26 schema:author N66d82ff8e2ed4e5ca284016731ef4fb2
27 schema:citation sg:pub.10.1007/bf00429780
28 sg:pub.10.1007/bf01222198
29 sg:pub.10.1007/s001250051198
30 sg:pub.10.1007/s004280050420
31 sg:pub.10.1038/nm0598-575
32 schema:datePublished 2002-01-01
33 schema:datePublishedReg 2002-01-01
34 schema:description The zucker diabetic fatty (ZDF-fa/fa) rat is one of the attractive models for type II diabetes based on impaired glucose tolerance caused by the inherited insulin-resistance gene fa. Characterization of nephropathy in this model may provide useful insights into the mechanism of the progression of diabetic nephropathy. The present study analyzed the pathophysiology of diabetes and nephropathy, including the process of glomerulosclerosis in this model by biochemical and morphometric analyses. In addition, we conducted studies in podocytes in culture to examine the direct effects of high glucose on podocytes. ZDF-fa/fa rats showed overt diabetes despite hyperinsulinemia as early as 3 months of age. Blood glucose levels increased further with a considerable decrease of insulin levels at 5 months. Glomerular filtration rate (GFR) was significantly elevated until 3 months, but fell to the level seen in lean rats by 7 months. Proteinuria started to rise during the period of increased GFR, and increased further after GFR had fallen to within the normal range. Renal fibronectin, collagen iv, and vascular endothelial growth factor mRNA levels were increased at 7 months. Glomerulosclerosis commenced as early as 5 months of age, and was associated with glomerular hypertrophy and mild mesangial expansion with evidence of accentuated podocyte injury, as revealed by increased expression of desmin. Electron microscopy suggested that degeneration of podocytes and the development of tuft adhesions were responsible for the glomerular sclerosis in this model. In addition, glomeruli from the diabetic rats showed up-regulation of the cyclin kinase inhibitors, p21 and p27. Further studies suggested that the increase in p27 expression was predominantly caused by podocytes, because predominant immunolocalization of p27 in podocytes in diabetic rats and high glucose medium induced cell hypertrophy accompanied by p27 up-regulation in differentiated podocyte cell lines. In conclusion, progressive diabetic nephropathy in ZDF-fa/fa rats is associated with evidence of podocyte injury. High concentrations of ambient glucose induced podocyte hypertrophy and stress in vitro, suggesting that the podocyte is a likely target of the diabetic milieu.
35 schema:genre article
36 schema:isAccessibleForFree true
37 schema:isPartOf N70f9afa8df4d443d9893d6a570556965
38 Nb16a95acdd8a45b4ac90564b1848c695
39 sg:journal.1017964
40 schema:keywords FA
41 Further studies
42 II diabetes
43 ZDF fa/fa rats
44 Zucker diabetic fatty rats
45 addition
46 adhesion
47 age
48 ambient glucose
49 analysis
50 attractive model
51 biochemical
52 blood glucose levels
53 cell hypertrophy
54 cell lines
55 characterization
56 collagen IV
57 concentration
58 conclusion
59 considerable decrease
60 culture
61 cyclin kinase inhibitor
62 decrease
63 degeneration
64 desmin
65 development
66 diabetes
67 diabetic fatty rats
68 diabetic milieu
69 diabetic nephropathy
70 diabetic rats
71 direct effect
72 effect
73 electron microscopy
74 evidence
75 expansion
76 expression
77 fa rats
78 factor mRNA levels
79 fatty rats
80 fibronectin
81 filtration rate
82 genes Fas
83 glomerular filtration rate
84 glomerular hypertrophy
85 glomerular sclerosis
86 glomeruli
87 glomerulosclerosis
88 glucose
89 glucose levels
90 glucose medium
91 glucose tolerance
92 growth factor mRNA levels
93 high concentrations
94 high glucose
95 high glucose medium
96 hyperinsulinemia
97 hypertrophy
98 immunolocalization
99 impaired glucose tolerance
100 increase
101 increased expression
102 inhibitors
103 injury
104 insights
105 insulin levels
106 kinase inhibitors
107 lean rats
108 levels
109 likely target
110 lines
111 mRNA levels
112 mechanism
113 medium
114 mesangial expansion
115 microscopy
116 mild mesangial expansion
117 milieu
118 model
119 months
120 months of age
121 morphometric analysis
122 nephropathy
123 normal range
124 p21
125 p27
126 p27 expression
127 pathophysiology
128 pathophysiology of diabetes
129 period
130 podocyte cell line
131 podocyte hypertrophy
132 podocyte injury
133 podocytes
134 present study
135 process
136 process of glomerulosclerosis
137 progression
138 progressive diabetic nephropathy
139 progressive nephropathy
140 proteinuria
141 range
142 rate
143 rats
144 regulation
145 renal fibronectin
146 sclerosis
147 stress
148 study
149 target
150 tolerance
151 tuft adhesion
152 type II diabetes
153 useful insights
154 vascular endothelial growth factor (VEGF) mRNA levels
155 schema:name Podocyte Injury Promotes Progressive Nephropathy in Zucker Diabetic Fatty Rats
156 schema:pagination 25-35
157 schema:productId N07de1a83fb244aed8720c859d241f534
158 N69041eda793542cf88f2fde602c5cf3a
159 Nef7deb846b2948c89dbfd46ce3d3dd87
160 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008745799
161 https://doi.org/10.1038/labinvest.3780392
162 schema:sdDatePublished 2022-08-04T16:55
163 schema:sdLicense https://scigraph.springernature.com/explorer/license/
164 schema:sdPublisher N559353c9536841f582d503bf432fc144
165 schema:url https://doi.org/10.1038/labinvest.3780392
166 sgo:license sg:explorer/license/
167 sgo:sdDataset articles
168 rdf:type schema:ScholarlyArticle
169 N0225f85a24cb43bdbb8b36df375f8d23 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Vascular Endothelial Growth Factors
171 rdf:type schema:DefinedTerm
172 N051c11ddab1c4053a9c672a58ac66c47 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
173 schema:name Rats, Zucker
174 rdf:type schema:DefinedTerm
175 N052e225982ca4b9a9d75fd80b54d3b30 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
176 schema:name Mice
177 rdf:type schema:DefinedTerm
178 N079d5b0ed7f3412481165880f1a44504 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
179 schema:name Collagen Type IV
180 rdf:type schema:DefinedTerm
181 N07de1a83fb244aed8720c859d241f534 schema:name dimensions_id
182 schema:value pub.1008745799
183 rdf:type schema:PropertyValue
184 N1630a57ecf554f0480f90b9e2ec57a7d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
185 schema:name In Vitro Techniques
186 rdf:type schema:DefinedTerm
187 N29a8c0f52497466899c2276f53301095 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
188 schema:name Diabetes Mellitus, Type 2
189 rdf:type schema:DefinedTerm
190 N35406d241def4573bc602bf8d64b2ab8 schema:affiliation grid-institutes:None
191 schema:familyName Sonoda
192 schema:givenName Jiro
193 rdf:type schema:Person
194 N3d842f96962943fdaf92f1d8a5b6635b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
195 schema:name Transcription, Genetic
196 rdf:type schema:DefinedTerm
197 N470d02b197ef40f38fc0290601f8531f rdf:first sg:person.01022702772.51
198 rdf:rest rdf:nil
199 N49d9c0c9f7c84bc0aa0cf0e07e17051c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
200 schema:name Rats
201 rdf:type schema:DefinedTerm
202 N4e5f014cb5b64e9c801b39b701196594 rdf:first sg:person.012664624074.29
203 rdf:rest Neb29afc9336f47aeae3226459cbee5ee
204 N536f04a3fe2c4b7b937a94164d0bed32 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
205 schema:name Cell Line
206 rdf:type schema:DefinedTerm
207 N559353c9536841f582d503bf432fc144 schema:name Springer Nature - SN SciGraph project
208 rdf:type schema:Organization
209 N55a60ee0804548aea0b93370fb48bb2e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
210 schema:name Vascular Endothelial Growth Factor A
211 rdf:type schema:DefinedTerm
212 N55a8cb259dc8450cae3f2e9d13f2bdaf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
213 schema:name Diabetic Nephropathies
214 rdf:type schema:DefinedTerm
215 N5ba1202ba220418cad86e055b86c1ce4 schema:affiliation grid-institutes:grid.410714.7
216 schema:familyName Inagaki
217 schema:givenName Tomoko
218 rdf:type schema:Person
219 N5d037b72dd5045e08da4696d704b2698 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
220 schema:name Aging
221 rdf:type schema:DefinedTerm
222 N63857fa224c2488dafcc69fbd17d20c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
223 schema:name Animals
224 rdf:type schema:DefinedTerm
225 N63b5f2996bd44fa7864d7e0e49540eb4 rdf:first N35406d241def4573bc602bf8d64b2ab8
226 rdf:rest N4e5f014cb5b64e9c801b39b701196594
227 N664004ddef4f48ed90899941134e41e2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
228 schema:name Insulin Resistance
229 rdf:type schema:DefinedTerm
230 N66d82ff8e2ed4e5ca284016731ef4fb2 rdf:first sg:person.01100676737.04
231 rdf:rest Ncf905f0dc3df4a1897b999306cec25b3
232 N6759eb77b6da47dfab66349c8ed7e961 rdf:first Ndb44f16350ed4cec8a2fb993c9b120c1
233 rdf:rest N6ac40ba350c74411b8bdc72f07d92d60
234 N69041eda793542cf88f2fde602c5cf3a schema:name doi
235 schema:value 10.1038/labinvest.3780392
236 rdf:type schema:PropertyValue
237 N69b8da9f99b2404597be74f2c54e436b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
238 schema:name Blood Glucose
239 rdf:type schema:DefinedTerm
240 N6ac40ba350c74411b8bdc72f07d92d60 rdf:first N5ba1202ba220418cad86e055b86c1ce4
241 rdf:rest N63b5f2996bd44fa7864d7e0e49540eb4
242 N6de85a671fdf4b5380edb4db0e6452f4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
243 schema:name Lymphokines
244 rdf:type schema:DefinedTerm
245 N70f9afa8df4d443d9893d6a570556965 schema:issueNumber 1
246 rdf:type schema:PublicationIssue
247 N7f3f6668cc4f4756932bf0da118443cc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
248 schema:name Kidney
249 rdf:type schema:DefinedTerm
250 Na720a7020b804959b73d1ce6c438d9ae schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
251 schema:name Endothelial Growth Factors
252 rdf:type schema:DefinedTerm
253 Na81d51d5af974a6383153b7d9c42cb48 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
254 schema:name Fibronectins
255 rdf:type schema:DefinedTerm
256 Nb16a95acdd8a45b4ac90564b1848c695 schema:volumeNumber 82
257 rdf:type schema:PublicationVolume
258 Nc03e37f782e34e71800040131a034cc9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
259 schema:name Glomerular Filtration Rate
260 rdf:type schema:DefinedTerm
261 Nc8b0724444ad47ab98634d9eb79d7eb5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
262 schema:name Disease Models, Animal
263 rdf:type schema:DefinedTerm
264 Ncf905f0dc3df4a1897b999306cec25b3 rdf:first sg:person.0615210032.01
265 rdf:rest N6759eb77b6da47dfab66349c8ed7e961
266 Nd43bdc0fb88d45489d428bf8dcead7d8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
267 schema:name Epithelial Cells
268 rdf:type schema:DefinedTerm
269 Ndb44f16350ed4cec8a2fb993c9b120c1 schema:affiliation grid-institutes:None
270 schema:familyName Yoshida
271 schema:givenName Fusayo
272 rdf:type schema:Person
273 Ne7aa4abbc7af42d89c7d12dbc4eaeccc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
274 schema:name Disease Progression
275 rdf:type schema:DefinedTerm
276 Neb29afc9336f47aeae3226459cbee5ee rdf:first sg:person.01147012137.66
277 rdf:rest N470d02b197ef40f38fc0290601f8531f
278 Nef7deb846b2948c89dbfd46ce3d3dd87 schema:name pubmed_id
279 schema:value 11796823
280 rdf:type schema:PropertyValue
281 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
282 schema:name Medical and Health Sciences
283 rdf:type schema:DefinedTerm
284 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
285 schema:name Clinical Sciences
286 rdf:type schema:DefinedTerm
287 sg:journal.1017964 schema:issn 0023-6837
288 1530-0307
289 schema:name Laboratory Investigation
290 schema:publisher Springer Nature
291 rdf:type schema:Periodical
292 sg:person.01022702772.51 schema:affiliation grid-institutes:grid.20515.33
293 schema:familyName Nagata
294 schema:givenName Michio
295 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01022702772.51
296 rdf:type schema:Person
297 sg:person.01100676737.04 schema:affiliation grid-institutes:grid.20515.33
298 schema:familyName Hoshi
299 schema:givenName Sachi
300 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01100676737.04
301 rdf:type schema:Person
302 sg:person.01147012137.66 schema:affiliation grid-institutes:None
303 schema:familyName Nomoto
304 schema:givenName Ken-ichi
305 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01147012137.66
306 rdf:type schema:Person
307 sg:person.012664624074.29 schema:affiliation grid-institutes:grid.20515.33
308 schema:familyName Watanabe
309 schema:givenName Teruo
310 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012664624074.29
311 rdf:type schema:Person
312 sg:person.0615210032.01 schema:affiliation grid-institutes:grid.20515.33
313 schema:familyName Shu
314 schema:givenName Yujing
315 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0615210032.01
316 rdf:type schema:Person
317 sg:pub.10.1007/bf00429780 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028158860
318 https://doi.org/10.1007/bf00429780
319 rdf:type schema:CreativeWork
320 sg:pub.10.1007/bf01222198 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010446785
321 https://doi.org/10.1007/bf01222198
322 rdf:type schema:CreativeWork
323 sg:pub.10.1007/s001250051198 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042150322
324 https://doi.org/10.1007/s001250051198
325 rdf:type schema:CreativeWork
326 sg:pub.10.1007/s004280050420 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039232099
327 https://doi.org/10.1007/s004280050420
328 rdf:type schema:CreativeWork
329 sg:pub.10.1038/nm0598-575 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043972437
330 https://doi.org/10.1038/nm0598-575
331 rdf:type schema:CreativeWork
332 grid-institutes:None schema:alternateName Department of Cardiovascular Research, Eisai Research Laboratories, Tsukuba, Japan
333 schema:name Department of Cardiovascular Research, Eisai Research Laboratories, Tsukuba, Japan
334 rdf:type schema:Organization
335 grid-institutes:grid.20515.33 schema:alternateName Clinical Medicine, University of Tsukuba, Tsukuba, Japan
336 Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan
337 schema:name Clinical Medicine, University of Tsukuba, Tsukuba, Japan
338 Department of Pathology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan
339 rdf:type schema:Organization
340 grid-institutes:grid.410714.7 schema:alternateName Department of Pathology, Showa University, Tokyo, Japan
341 schema:name Department of Pathology, Showa University, Tokyo, Japan
342 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...