Ontology type: schema:ScholarlyArticle Open Access: True
2004-05-03
AUTHORSTomonaga Ichikawa, Shuji Kitajima, Jingyan Liang, Tomonari Koike, Xiaofei Wang, Huijun Sun, Mitsuyo Okazaki, Masatoshi Morimoto, Hisataka Shikama, Teruo Watanabe, Nobuhiro Yamada, Jianglin Fan
ABSTRACTLipoprotein lipase (LPL) is a key enzyme in the hydrolysis of triglyceride-rich lipoproteins. Previous studies using transgenic mice and rabbits have demonstrated that high level of LPL activity in adipose and skeletal muscle protects against diet-induced hypercholesterolemia and subsequently prevents aortic atherosclerosis. However, it is unknown, per se, whether increased LPL activity itself is antiatherogenic, or whether the antiatherogenic effect of LPL is dependent upon the LPL lipid-lowering effect. To address this issue, we fed LPL transgenic and littermate rabbits diets containing different amounts of cholesterol (0.3–0.6%) adjusted to maintain their plasma cholesterol concentrations at similarly high levels for 16 weeks. We analyzed their lipoprotein profiles and compared their susceptibility to atherosclerosis. The results showed that the overexpression of LPL in transgenic rabbits reduced remnant lipoproteins (β-VLDL, d<1.006 g/ml) but concomitantly led to a significant increase of the large (d=1.02–1.04 g/ml) and small LDLs (d=1.04–1.06 g/ml) compared to the amounts in control rabbits. Furthermore, we found that with equally high hypercholesterolemia, transgenic rabbits developed 1.8-fold more extensive aortic atherosclerosis than control rabbits. To examine the hypothesis that altered lipoprotein profiles may be responsible for the enhanced atherosclerosis in transgenic rabbits, we studied the atherogenic properties of apoB-containing lipoproteins in vitro. These studies revealed that small-sized LDLs of transgenic rabbits were more susceptible to copper-induced oxidation and had higher affinity to biglycan than large remnant lipoproteins. We conclude, therefore, that LPL exerts a dual function in terms of its atherogenicity, namely antiatherogenicity, through enhancing receptor-mediated remnant lipoprotein catabolism and proatherogenicity via the generation of a large amount of small-sized LDLs. At an equal atherogenic-cholesterol level, small and dense LDLs are more atherogenic than large remnant lipoproteins. More... »
PAGES715-726
http://scigraph.springernature.com/pub.10.1038/labinvest.3700102
DOIhttp://dx.doi.org/10.1038/labinvest.3700102
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1034295961
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/15122303
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1101",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical Biochemistry and Metabolomics",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals, Genetically Modified",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Aorta",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Apolipoproteins B",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Arteriosclerosis",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Gene Expression",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Lipoprotein Lipase",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Lipoproteins, LDL",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Lipoproteins, VLDL",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Male",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Microscopy, Electron",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Rabbits",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Recombinant Proteins",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Department of Pathology, Cardiovascular Disease Laboratory, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan",
"id": "http://www.grid.ac/institutes/grid.20515.33",
"name": [
"Department of Pathology, Cardiovascular Disease Laboratory, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan"
],
"type": "Organization"
},
"familyName": "Ichikawa",
"givenName": "Tomonaga",
"id": "sg:person.0625674757.78",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0625674757.78"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Analytical Research Center for Experimental Sciences, Saga University, Saga, Japan",
"id": "http://www.grid.ac/institutes/grid.412339.e",
"name": [
"Analytical Research Center for Experimental Sciences, Saga University, Saga, Japan"
],
"type": "Organization"
},
"familyName": "Kitajima",
"givenName": "Shuji",
"id": "sg:person.0776250500.12",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776250500.12"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Pathology, Cardiovascular Disease Laboratory, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan",
"id": "http://www.grid.ac/institutes/grid.20515.33",
"name": [
"Department of Pathology, Cardiovascular Disease Laboratory, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan"
],
"type": "Organization"
},
"familyName": "Liang",
"givenName": "Jingyan",
"id": "sg:person.01172340432.80",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01172340432.80"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Pathology, Cardiovascular Disease Laboratory, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan",
"id": "http://www.grid.ac/institutes/grid.20515.33",
"name": [
"Department of Pathology, Cardiovascular Disease Laboratory, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan"
],
"type": "Organization"
},
"familyName": "Koike",
"givenName": "Tomonari",
"id": "sg:person.011340333222.04",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011340333222.04"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Pathology, Cardiovascular Disease Laboratory, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan",
"id": "http://www.grid.ac/institutes/grid.20515.33",
"name": [
"Department of Pathology, Cardiovascular Disease Laboratory, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan"
],
"type": "Organization"
},
"familyName": "Wang",
"givenName": "Xiaofei",
"id": "sg:person.01300565244.96",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01300565244.96"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Pharmacology, Dalian Medical University, Dalian, China",
"id": "http://www.grid.ac/institutes/grid.411971.b",
"name": [
"Department of Pathology, Cardiovascular Disease Laboratory, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan",
"Department of Pharmacology, Dalian Medical University, Dalian, China"
],
"type": "Organization"
},
"familyName": "Sun",
"givenName": "Huijun",
"id": "sg:person.0762316763.77",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0762316763.77"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Laboratory of Chemistry, College of Liberal Arts and Sciences, Tokyo Medical and Dental University, Tokyo, Japan",
"id": "http://www.grid.ac/institutes/grid.265073.5",
"name": [
"Laboratory of Chemistry, College of Liberal Arts and Sciences, Tokyo Medical and Dental University, Tokyo, Japan"
],
"type": "Organization"
},
"familyName": "Okazaki",
"givenName": "Mitsuyo",
"id": "sg:person.016621004641.41",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016621004641.41"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Analytical Research Center for Experimental Sciences, Saga University, Saga, Japan",
"id": "http://www.grid.ac/institutes/grid.412339.e",
"name": [
"Analytical Research Center for Experimental Sciences, Saga University, Saga, Japan"
],
"type": "Organization"
},
"familyName": "Morimoto",
"givenName": "Masatoshi",
"id": "sg:person.013573100061.38",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013573100061.38"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Yamanouchi Pharmaceutical Company, Tsukuba Research Institute, Tsukuba, Japan",
"id": "http://www.grid.ac/institutes/None",
"name": [
"Yamanouchi Pharmaceutical Company, Tsukuba Research Institute, Tsukuba, Japan"
],
"type": "Organization"
},
"familyName": "Shikama",
"givenName": "Hisataka",
"id": "sg:person.01054341145.34",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01054341145.34"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Saga University, Saga, Japan",
"id": "http://www.grid.ac/institutes/grid.412339.e",
"name": [
"Saga University, Saga, Japan"
],
"type": "Organization"
},
"familyName": "Watanabe",
"givenName": "Teruo",
"id": "sg:person.012664624074.29",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012664624074.29"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Division of Metabolism and Endocrinology, Institute of Clinical Medicine, University of Tsukuba, Tsukuba, Japan",
"id": "http://www.grid.ac/institutes/grid.20515.33",
"name": [
"Division of Metabolism and Endocrinology, Institute of Clinical Medicine, University of Tsukuba, Tsukuba, Japan"
],
"type": "Organization"
},
"familyName": "Yamada",
"givenName": "Nobuhiro",
"id": "sg:person.010517636062.98",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010517636062.98"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Pathology, Cardiovascular Disease Laboratory, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan",
"id": "http://www.grid.ac/institutes/grid.20515.33",
"name": [
"Department of Pathology, Cardiovascular Disease Laboratory, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan"
],
"type": "Organization"
},
"familyName": "Fan",
"givenName": "Jianglin",
"id": "sg:person.01136672602.49",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01136672602.49"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1038/nm1102-1211",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036871954",
"https://doi.org/10.1038/nm1102-1211"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nature00804",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047268212",
"https://doi.org/10.1038/nature00804"
],
"type": "CreativeWork"
}
],
"datePublished": "2004-05-03",
"datePublishedReg": "2004-05-03",
"description": "Lipoprotein lipase (LPL) is a key enzyme in the hydrolysis of triglyceride-rich lipoproteins. Previous studies using transgenic mice and rabbits have demonstrated that high level of LPL activity in adipose and skeletal muscle protects against diet-induced hypercholesterolemia and subsequently prevents aortic atherosclerosis. However, it is unknown, per se, whether increased LPL activity itself is antiatherogenic, or whether the antiatherogenic effect of LPL is dependent upon the LPL lipid-lowering effect. To address this issue, we fed LPL transgenic and littermate rabbits diets containing different amounts of cholesterol (0.3\u20130.6%) adjusted to maintain their plasma cholesterol concentrations at similarly high levels for 16 weeks. We analyzed their lipoprotein profiles and compared their susceptibility to atherosclerosis. The results showed that the overexpression of LPL in transgenic rabbits reduced remnant lipoproteins (\u03b2-VLDL, d<1.006\u2009g/ml) but concomitantly led to a significant increase of the large (d=1.02\u20131.04\u2009g/ml) and small LDLs (d=1.04\u20131.06\u2009g/ml) compared to the amounts in control rabbits. Furthermore, we found that with equally high hypercholesterolemia, transgenic rabbits developed 1.8-fold more extensive aortic atherosclerosis than control rabbits. To examine the hypothesis that altered lipoprotein profiles may be responsible for the enhanced atherosclerosis in transgenic rabbits, we studied the atherogenic properties of apoB-containing lipoproteins in vitro. These studies revealed that small-sized LDLs of transgenic rabbits were more susceptible to copper-induced oxidation and had higher affinity to biglycan than large remnant lipoproteins. We conclude, therefore, that LPL exerts a dual function in terms of its atherogenicity, namely antiatherogenicity, through enhancing receptor-mediated remnant lipoprotein catabolism and proatherogenicity via the generation of a large amount of small-sized LDLs. At an equal atherogenic-cholesterol level, small and dense LDLs are more atherogenic than large remnant lipoproteins.",
"genre": "article",
"id": "sg:pub.10.1038/labinvest.3700102",
"inLanguage": "en",
"isAccessibleForFree": true,
"isFundedItemOf": [
{
"id": "sg:grant.6262693",
"type": "MonetaryGrant"
}
],
"isPartOf": [
{
"id": "sg:journal.1017964",
"issn": [
"0023-6837",
"1530-0307"
],
"name": "Laboratory Investigation",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "6",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "84"
}
],
"keywords": [
"small-sized LDL",
"remnant lipoproteins",
"lipoprotein lipase",
"aortic atherosclerosis",
"lipoprotein profile",
"dense LDL",
"transgenic rabbits",
"control rabbits",
"LPL activity",
"overexpression of LPL",
"extensive aortic atherosclerosis",
"atherogenic cholesterol levels",
"diet-induced hypercholesterolemia",
"lipid-lowering effects",
"small dense LDL",
"plasma cholesterol concentration",
"triglyceride-rich lipoproteins",
"apoB-containing lipoproteins",
"higher hypercholesterolemia",
"enhanced atherosclerosis",
"copper-induced oxidation",
"antiatherogenic effects",
"small LDL",
"atherogenic properties",
"lipoprotein catabolism",
"skeletal muscle protects",
"cholesterol concentrations",
"transgenic mice",
"LDL",
"atherosclerosis",
"lipoproteins",
"rabbit diets",
"rabbits",
"high levels",
"hypercholesterolemia",
"significant increase",
"overexpression",
"high affinity",
"proatherogenicity",
"previous studies",
"antiatherogenicity",
"atherogenicity",
"levels",
"key enzyme",
"mice",
"protects",
"cholesterol",
"weeks",
"adipose",
"diet",
"activity",
"study",
"biglycan",
"lipase",
"catabolism",
"effect",
"profile",
"susceptibility",
"dual function",
"plasma",
"increase",
"enzyme",
"hypothesis",
"concentration",
"amount",
"affinity",
"function",
"large amount",
"results",
"different amounts",
"hydrolysis",
"issues",
"generation",
"terms",
"oxidation",
"properties"
],
"name": "Overexpression of lipoprotein lipase in transgenic rabbits leads to increased small dense LDL in plasma and promotes atherosclerosis",
"pagination": "715-726",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1034295961"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1038/labinvest.3700102"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"15122303"
]
}
],
"sameAs": [
"https://doi.org/10.1038/labinvest.3700102",
"https://app.dimensions.ai/details/publication/pub.1034295961"
],
"sdDataset": "articles",
"sdDatePublished": "2022-06-01T22:04",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220601/entities/gbq_results/article/article_379.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1038/labinvest.3700102"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/labinvest.3700102'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/labinvest.3700102'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/labinvest.3700102'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/labinvest.3700102'
This table displays all metadata directly associated to this object as RDF triples.
298 TRIPLES
22 PREDICATES
118 URIs
108 LITERALS
21 BLANK NODES