Quantitative assessment shows loss of antigenic epitopes as a function of pre-analytic variables View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2011-08

AUTHORS

Yalai Bai, Juliana Tolles, Huan Cheng, Summar Siddiqui, Arun Gopinath, Eirini Pectasides, Robert L Camp, David L Rimm, Annette M Molinaro

ABSTRACT

Pre-analytic variables, specifically cold ischemic time, have been implicated as key variables in the measurement of proteins by immunohistochemistry. To determine the significance and magnitude of antigenic loss due to pre-analytic variables, we have compared protein antigenicity in core needle biopsies, with essentially no cold ischemic time, with that in routinely processed tumor resection specimens. Two cohorts of matched core needle biopsies and tumor resections were collected with 20 matched pairs and 14 matched pairs, respectively. Both series were analyzed by quantitative immunofluorescence using the AQUA® method. Epitopes phospho-ERK, total ERK, phospho-AKT, total AKT, phospho-S6K1, total S6K1, estrogen receptor (ER), Ki67, cytokeratin and GAPDH were assessed. Detection levels for all phospho-epitopes were significantly decreased in tumor resections compared with biopsies while no significant change was seen in the corresponding total proteins. Of the other four proteins examined, ER and cytokeratin showed significant loss of antigenicity. This data suggest that measurement of phospho-protein antigenicity in formalin-fixed tissue by immunological methods is dramatically affected by pre-analytic variables. This study suggests that core needle biopsies are more accurate for assessment of tissue biomarkers. More... »

PAGES

1253

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/labinvest.2011.75

DOI

http://dx.doi.org/10.1038/labinvest.2011.75

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1000345446

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21519325


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curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/labinvest.2011.75'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/labinvest.2011.75'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/labinvest.2011.75'


 

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