Safety and blood sample volume and quality of a refined retro-orbital bleeding technique in rats using a lateral approach View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2014-02

AUTHORS

Ashish Sharma, Brian L. Fish, Eric P. Cohen, John E. Baker, John E. Moulder, Marylou Mader, Meetha Medhora

ABSTRACT

The collection of blood samples from laboratory rats requires the use of bleeding techniques that provide quality samples of sufficient volume for analysis without injury to the animal. Retro-orbital bleeding (ROB) is a phlebotomy technique that can yield high-quality samples of adequate volume, but it has been criticized for its potential to cause injury. To evaluate the injury-causing potential of their refined ROB method using a lateral approach, the authors retrospectively reviewed ROB procedures carried out in their colony during an 18-month period and found that 0.6% of these procedures were associated with ocular injury. The authors also compared the quality of blood samples collected by ROB and by saphenous phlebotomy and found that ROB yielded samples of better quality. The authors conclude that, when done using a lateral approach and by an experienced technician, ROB is humane and safe and provides blood samples of adequate volume and quality for analysis. More... »

PAGES

63

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/laban.432

DOI

http://dx.doi.org/10.1038/laban.432

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1002445973

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24451361


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