A role for P selectin in complement-independent neutrophil-mediated glomerular injury View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1994-07

AUTHORS

P G Tipping, X R Huang, M C Berndt, S R Holdsworth

ABSTRACT

Neutrophil recruitment and lung injury following complement activation have been demonstrated to be dependent on endothelial expression of P selectin. In anti-glomerular basement membrane antibody-induced glomerulonephritis (anti-GBM GN) in mice, acute glomerular injury results from complement-independent neutrophil accumulation. The signals for neutrophil recruitment in this model are unknown. Expression of P selectin on glomerular endothelium was demonstrated within 30 minutes of administration of anti-GBM antibody to C57/BL10 mice. This was associated with rapid accumulation of neutrophils in glomeruli which peaked at one hour (6.2 +/- 0.5 neutrophils per glomerular cross section [neut/gcs], normal 0.34 +/- 0.06 neut/gcs, P < 0.01) and significant proteinuria after 16 hours (3.6 +/- 0.5 mg/16 hr, control 0.62 +/- 0.13 mg/16 hr, P < 0.01). Complement depletion with cobra venom factor, which reduced serum C3 levels to less than 5% of normal, did not alter expression of P selectin, reduce glomerular neutrophil accumulation (6.7 +/- 0.8 neut/gcs) or proteinuria (3.7 +/- 0.5 mg/16 hr). Platelet depletion also failed to alter glomerular expression of P selectin, neutrophil accumulation or the development of proteinuria. Mice were treated with an affinity purified anti-human P selectin antibody, which cross reacted with mouse P selectin and blocked P selectin-dependent binding of thrombin-activated mouse platelets to HL60 cells and did not bind to mouse neutrophils. Treatment, one hour prior to the administration of anti-GBM antibody, markedly inhibited glomerular neutrophil accumulation (0.94 +/- 0.12 neut/gcs) and prevented proteinuria (1.0 +/- 0.2 mg/16 hr), and did not alter binding of anti-GBM globulin in the kidney. These data strongly suggest that the rapid up-regulation of P selectin expression in glomeruli following binding of anti-GBM antibody is an essential signal for neutrophil recruitment in this complement independent model of glomerular injury. More... »

PAGES

79-88

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/ki.1994.246

DOI

http://dx.doi.org/10.1038/ki.1994.246

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1006373571

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/7523757


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Immunology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Basement Membrane", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Blood Platelets", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Complement C3", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glomerulonephritis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kidney Glomerulus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Leukocyte Count", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Inbred C57BL", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neutrophils", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "P-Selectin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Platelet Membrane Glycoproteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Up-Regulation", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Monash Medical Centre", 
          "id": "https://www.grid.ac/institutes/grid.416060.5", 
          "name": [
            "Monash University, Department of Medicine, Monash Medical Centre, Clayton, Victoria, Australia."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tipping", 
        "givenName": "P G", 
        "id": "sg:person.0661674330.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0661674330.39"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Huang", 
        "givenName": "X R", 
        "id": "sg:person.01233272432.56", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01233272432.56"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Berndt", 
        "givenName": "M C", 
        "id": "sg:person.01363134417.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363134417.11"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Holdsworth", 
        "givenName": "S R", 
        "id": "sg:person.01131260677.45", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01131260677.45"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1161/01.str.25.1.202", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002579660"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/359848a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002638369", 
          "https://doi.org/10.1038/359848a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ki.1986.260", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003199776", 
          "https://doi.org/10.1038/ki.1986.260"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(93)80055-j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004344510"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci113407", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008248286"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.112.4.749", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009351186"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci116029", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013353899"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.124.4.733", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019815261"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/343757a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023114426", 
          "https://doi.org/10.1038/343757a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.177.3.667", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023706904"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(76)90527-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025529346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci114175", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026244285"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.175.4.1147", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026577196"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci114111", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026924458"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(89)90292-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032801067"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci116237", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033282179"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4612-3234-6_6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037926761", 
          "https://doi.org/10.1007/978-1-4612-3234-6_6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0167-5699(92)90149-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038923122"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-291x(91)90511-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040343100"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci116210", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043231419"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci112965", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046377836"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ki.1990.171", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050237268", 
          "https://doi.org/10.1038/ki.1990.171"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00041552-199303000-00001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051422642"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00041552-199303000-00001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051422642"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075305601", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075765083", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076015278", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077314062", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077946733", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078887574", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078892035", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078892056", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079495399", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079525479", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079809638", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajpheart.1987.253.3.h699", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1079861635"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079901148", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080729829", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081562088", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082648588", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1994-07", 
    "datePublishedReg": "1994-07-01", 
    "description": "Neutrophil recruitment and lung injury following complement activation have been demonstrated to be dependent on endothelial expression of P selectin. In anti-glomerular basement membrane antibody-induced glomerulonephritis (anti-GBM GN) in mice, acute glomerular injury results from complement-independent neutrophil accumulation. The signals for neutrophil recruitment in this model are unknown. Expression of P selectin on glomerular endothelium was demonstrated within 30 minutes of administration of anti-GBM antibody to C57/BL10 mice. This was associated with rapid accumulation of neutrophils in glomeruli which peaked at one hour (6.2 +/- 0.5 neutrophils per glomerular cross section [neut/gcs], normal 0.34 +/- 0.06 neut/gcs, P < 0.01) and significant proteinuria after 16 hours (3.6 +/- 0.5 mg/16 hr, control 0.62 +/- 0.13 mg/16 hr, P < 0.01). Complement depletion with cobra venom factor, which reduced serum C3 levels to less than 5% of normal, did not alter expression of P selectin, reduce glomerular neutrophil accumulation (6.7 +/- 0.8 neut/gcs) or proteinuria (3.7 +/- 0.5 mg/16 hr). Platelet depletion also failed to alter glomerular expression of P selectin, neutrophil accumulation or the development of proteinuria. Mice were treated with an affinity purified anti-human P selectin antibody, which cross reacted with mouse P selectin and blocked P selectin-dependent binding of thrombin-activated mouse platelets to HL60 cells and did not bind to mouse neutrophils. Treatment, one hour prior to the administration of anti-GBM antibody, markedly inhibited glomerular neutrophil accumulation (0.94 +/- 0.12 neut/gcs) and prevented proteinuria (1.0 +/- 0.2 mg/16 hr), and did not alter binding of anti-GBM globulin in the kidney. These data strongly suggest that the rapid up-regulation of P selectin expression in glomeruli following binding of anti-GBM antibody is an essential signal for neutrophil recruitment in this complement independent model of glomerular injury.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/ki.1994.246", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1015319", 
        "issn": [
          "0085-2538", 
          "1523-1755"
        ], 
        "name": "Kidney International", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "46"
      }
    ], 
    "name": "A role for P selectin in complement-independent neutrophil-mediated glomerular injury", 
    "pagination": "79-88", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "1e5bddc3906cb93821572474a2f24f783257a470e02eeb49917437ae54bf892a"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "7523757"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0323470"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/ki.1994.246"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1006373571"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/ki.1994.246", 
      "https://app.dimensions.ai/details/publication/pub.1006373571"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T18:56", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8678_00000421.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/ki/journal/v46/n1/abs/ki1994246a.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/ki.1994.246'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/ki.1994.246'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/ki.1994.246'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/ki.1994.246'


 

This table displays all metadata directly associated to this object as RDF triples.

250 TRIPLES      21 PREDICATES      82 URIs      35 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/ki.1994.246 schema:about N04dd4d49b12c4bfc9de9781d29ba938a
2 N21ad0a926d1241c2811a2d1dd8f774bf
3 N241f4b650e314188ade99cd20a720da7
4 N38c441620a804ddf93750a99c7c17892
5 N419f085c349e4603b24c131b75789ff1
6 N5c565dea3f1747e2ada41468dbea4d0c
7 N634f22d341d047e99a38eb707914de5b
8 N636fe9fc8a10478b9fd7837449a61394
9 N78e8912203cd4dab8c208790aa328cb0
10 N810ac492d0704612a70fa66629076688
11 Na7785559e30a4497912cae105e594475
12 Nb62c6a4de5584fa49839b8676c492e34
13 Nc135578d6e014f72aa50d58dfdfbbfce
14 Nf95b6f311cac4f87a5e9563f9f098345
15 anzsrc-for:11
16 anzsrc-for:1107
17 schema:author Nb6280213406a4211aa5fcc4162e0dd47
18 schema:citation sg:pub.10.1007/978-1-4612-3234-6_6
19 sg:pub.10.1038/343757a0
20 sg:pub.10.1038/359848a0
21 sg:pub.10.1038/ki.1986.260
22 sg:pub.10.1038/ki.1990.171
23 https://app.dimensions.ai/details/publication/pub.1075305601
24 https://app.dimensions.ai/details/publication/pub.1075765083
25 https://app.dimensions.ai/details/publication/pub.1076015278
26 https://app.dimensions.ai/details/publication/pub.1077314062
27 https://app.dimensions.ai/details/publication/pub.1077946733
28 https://app.dimensions.ai/details/publication/pub.1078887574
29 https://app.dimensions.ai/details/publication/pub.1078892035
30 https://app.dimensions.ai/details/publication/pub.1078892056
31 https://app.dimensions.ai/details/publication/pub.1079495399
32 https://app.dimensions.ai/details/publication/pub.1079525479
33 https://app.dimensions.ai/details/publication/pub.1079809638
34 https://app.dimensions.ai/details/publication/pub.1079901148
35 https://app.dimensions.ai/details/publication/pub.1080729829
36 https://app.dimensions.ai/details/publication/pub.1081562088
37 https://app.dimensions.ai/details/publication/pub.1082648588
38 https://doi.org/10.1016/0003-2697(76)90527-3
39 https://doi.org/10.1016/0006-291x(91)90511-5
40 https://doi.org/10.1016/0092-8674(89)90292-4
41 https://doi.org/10.1016/0092-8674(93)80055-j
42 https://doi.org/10.1016/0167-5699(92)90149-2
43 https://doi.org/10.1083/jcb.112.4.749
44 https://doi.org/10.1084/jem.124.4.733
45 https://doi.org/10.1084/jem.175.4.1147
46 https://doi.org/10.1084/jem.177.3.667
47 https://doi.org/10.1097/00041552-199303000-00001
48 https://doi.org/10.1152/ajpheart.1987.253.3.h699
49 https://doi.org/10.1161/01.str.25.1.202
50 https://doi.org/10.1172/jci112965
51 https://doi.org/10.1172/jci113407
52 https://doi.org/10.1172/jci114111
53 https://doi.org/10.1172/jci114175
54 https://doi.org/10.1172/jci116029
55 https://doi.org/10.1172/jci116210
56 https://doi.org/10.1172/jci116237
57 schema:datePublished 1994-07
58 schema:datePublishedReg 1994-07-01
59 schema:description Neutrophil recruitment and lung injury following complement activation have been demonstrated to be dependent on endothelial expression of P selectin. In anti-glomerular basement membrane antibody-induced glomerulonephritis (anti-GBM GN) in mice, acute glomerular injury results from complement-independent neutrophil accumulation. The signals for neutrophil recruitment in this model are unknown. Expression of P selectin on glomerular endothelium was demonstrated within 30 minutes of administration of anti-GBM antibody to C57/BL10 mice. This was associated with rapid accumulation of neutrophils in glomeruli which peaked at one hour (6.2 +/- 0.5 neutrophils per glomerular cross section [neut/gcs], normal 0.34 +/- 0.06 neut/gcs, P < 0.01) and significant proteinuria after 16 hours (3.6 +/- 0.5 mg/16 hr, control 0.62 +/- 0.13 mg/16 hr, P < 0.01). Complement depletion with cobra venom factor, which reduced serum C3 levels to less than 5% of normal, did not alter expression of P selectin, reduce glomerular neutrophil accumulation (6.7 +/- 0.8 neut/gcs) or proteinuria (3.7 +/- 0.5 mg/16 hr). Platelet depletion also failed to alter glomerular expression of P selectin, neutrophil accumulation or the development of proteinuria. Mice were treated with an affinity purified anti-human P selectin antibody, which cross reacted with mouse P selectin and blocked P selectin-dependent binding of thrombin-activated mouse platelets to HL60 cells and did not bind to mouse neutrophils. Treatment, one hour prior to the administration of anti-GBM antibody, markedly inhibited glomerular neutrophil accumulation (0.94 +/- 0.12 neut/gcs) and prevented proteinuria (1.0 +/- 0.2 mg/16 hr), and did not alter binding of anti-GBM globulin in the kidney. These data strongly suggest that the rapid up-regulation of P selectin expression in glomeruli following binding of anti-GBM antibody is an essential signal for neutrophil recruitment in this complement independent model of glomerular injury.
60 schema:genre research_article
61 schema:inLanguage en
62 schema:isAccessibleForFree true
63 schema:isPartOf N06d0b02baed24343ab5386e4372ffe8b
64 N5f7f58e40faa4b2ca321aca468401ab2
65 sg:journal.1015319
66 schema:name A role for P selectin in complement-independent neutrophil-mediated glomerular injury
67 schema:pagination 79-88
68 schema:productId N4ca65a1b57264b998cf821ff3a7da179
69 N5bce1efb1b114e95a0d742f97a82a320
70 Nb2384c953e3f4b0985d5382329a372e2
71 Ncd9da23d4eee4ca697a71868cb7ea40f
72 Nf7e8561d98b84b70b5edf8c7f7682558
73 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006373571
74 https://doi.org/10.1038/ki.1994.246
75 schema:sdDatePublished 2019-04-10T18:56
76 schema:sdLicense https://scigraph.springernature.com/explorer/license/
77 schema:sdPublisher N22dc83e63d0545e5bdfc27984a3ca133
78 schema:url http://www.nature.com/ki/journal/v46/n1/abs/ki1994246a.html
79 sgo:license sg:explorer/license/
80 sgo:sdDataset articles
81 rdf:type schema:ScholarlyArticle
82 N04dd4d49b12c4bfc9de9781d29ba938a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Glomerulonephritis
84 rdf:type schema:DefinedTerm
85 N06d0b02baed24343ab5386e4372ffe8b schema:issueNumber 1
86 rdf:type schema:PublicationIssue
87 N21ad0a926d1241c2811a2d1dd8f774bf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Platelet Membrane Glycoproteins
89 rdf:type schema:DefinedTerm
90 N22dc83e63d0545e5bdfc27984a3ca133 schema:name Springer Nature - SN SciGraph project
91 rdf:type schema:Organization
92 N241f4b650e314188ade99cd20a720da7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Neutrophils
94 rdf:type schema:DefinedTerm
95 N38c441620a804ddf93750a99c7c17892 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Kidney Glomerulus
97 rdf:type schema:DefinedTerm
98 N419f085c349e4603b24c131b75789ff1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Male
100 rdf:type schema:DefinedTerm
101 N4ca65a1b57264b998cf821ff3a7da179 schema:name pubmed_id
102 schema:value 7523757
103 rdf:type schema:PropertyValue
104 N5bce1efb1b114e95a0d742f97a82a320 schema:name nlm_unique_id
105 schema:value 0323470
106 rdf:type schema:PropertyValue
107 N5c565dea3f1747e2ada41468dbea4d0c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Leukocyte Count
109 rdf:type schema:DefinedTerm
110 N5f7f58e40faa4b2ca321aca468401ab2 schema:volumeNumber 46
111 rdf:type schema:PublicationVolume
112 N634f22d341d047e99a38eb707914de5b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Animals
114 rdf:type schema:DefinedTerm
115 N636fe9fc8a10478b9fd7837449a61394 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Complement C3
117 rdf:type schema:DefinedTerm
118 N78e8912203cd4dab8c208790aa328cb0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Up-Regulation
120 rdf:type schema:DefinedTerm
121 N810ac492d0704612a70fa66629076688 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Mice, Inbred C57BL
123 rdf:type schema:DefinedTerm
124 Na7785559e30a4497912cae105e594475 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Basement Membrane
126 rdf:type schema:DefinedTerm
127 Nb2384c953e3f4b0985d5382329a372e2 schema:name dimensions_id
128 schema:value pub.1006373571
129 rdf:type schema:PropertyValue
130 Nb6280213406a4211aa5fcc4162e0dd47 rdf:first sg:person.0661674330.39
131 rdf:rest Ndc9a332d3c86474ea0efbca7f9a554f4
132 Nb62c6a4de5584fa49839b8676c492e34 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name P-Selectin
134 rdf:type schema:DefinedTerm
135 Nc135578d6e014f72aa50d58dfdfbbfce schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Mice
137 rdf:type schema:DefinedTerm
138 Nc52fa03ddf5645bdaa10b4477350afbf rdf:first sg:person.01363134417.11
139 rdf:rest Nf55a7112f5e047929e030c44a43e6394
140 Ncd9da23d4eee4ca697a71868cb7ea40f schema:name readcube_id
141 schema:value 1e5bddc3906cb93821572474a2f24f783257a470e02eeb49917437ae54bf892a
142 rdf:type schema:PropertyValue
143 Ndc9a332d3c86474ea0efbca7f9a554f4 rdf:first sg:person.01233272432.56
144 rdf:rest Nc52fa03ddf5645bdaa10b4477350afbf
145 Nf55a7112f5e047929e030c44a43e6394 rdf:first sg:person.01131260677.45
146 rdf:rest rdf:nil
147 Nf7e8561d98b84b70b5edf8c7f7682558 schema:name doi
148 schema:value 10.1038/ki.1994.246
149 rdf:type schema:PropertyValue
150 Nf95b6f311cac4f87a5e9563f9f098345 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Blood Platelets
152 rdf:type schema:DefinedTerm
153 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
154 schema:name Medical and Health Sciences
155 rdf:type schema:DefinedTerm
156 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
157 schema:name Immunology
158 rdf:type schema:DefinedTerm
159 sg:journal.1015319 schema:issn 0085-2538
160 1523-1755
161 schema:name Kidney International
162 rdf:type schema:Periodical
163 sg:person.01131260677.45 schema:familyName Holdsworth
164 schema:givenName S R
165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01131260677.45
166 rdf:type schema:Person
167 sg:person.01233272432.56 schema:familyName Huang
168 schema:givenName X R
169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01233272432.56
170 rdf:type schema:Person
171 sg:person.01363134417.11 schema:familyName Berndt
172 schema:givenName M C
173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363134417.11
174 rdf:type schema:Person
175 sg:person.0661674330.39 schema:affiliation https://www.grid.ac/institutes/grid.416060.5
176 schema:familyName Tipping
177 schema:givenName P G
178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0661674330.39
179 rdf:type schema:Person
180 sg:pub.10.1007/978-1-4612-3234-6_6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037926761
181 https://doi.org/10.1007/978-1-4612-3234-6_6
182 rdf:type schema:CreativeWork
183 sg:pub.10.1038/343757a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023114426
184 https://doi.org/10.1038/343757a0
185 rdf:type schema:CreativeWork
186 sg:pub.10.1038/359848a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002638369
187 https://doi.org/10.1038/359848a0
188 rdf:type schema:CreativeWork
189 sg:pub.10.1038/ki.1986.260 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003199776
190 https://doi.org/10.1038/ki.1986.260
191 rdf:type schema:CreativeWork
192 sg:pub.10.1038/ki.1990.171 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050237268
193 https://doi.org/10.1038/ki.1990.171
194 rdf:type schema:CreativeWork
195 https://app.dimensions.ai/details/publication/pub.1075305601 schema:CreativeWork
196 https://app.dimensions.ai/details/publication/pub.1075765083 schema:CreativeWork
197 https://app.dimensions.ai/details/publication/pub.1076015278 schema:CreativeWork
198 https://app.dimensions.ai/details/publication/pub.1077314062 schema:CreativeWork
199 https://app.dimensions.ai/details/publication/pub.1077946733 schema:CreativeWork
200 https://app.dimensions.ai/details/publication/pub.1078887574 schema:CreativeWork
201 https://app.dimensions.ai/details/publication/pub.1078892035 schema:CreativeWork
202 https://app.dimensions.ai/details/publication/pub.1078892056 schema:CreativeWork
203 https://app.dimensions.ai/details/publication/pub.1079495399 schema:CreativeWork
204 https://app.dimensions.ai/details/publication/pub.1079525479 schema:CreativeWork
205 https://app.dimensions.ai/details/publication/pub.1079809638 schema:CreativeWork
206 https://app.dimensions.ai/details/publication/pub.1079901148 schema:CreativeWork
207 https://app.dimensions.ai/details/publication/pub.1080729829 schema:CreativeWork
208 https://app.dimensions.ai/details/publication/pub.1081562088 schema:CreativeWork
209 https://app.dimensions.ai/details/publication/pub.1082648588 schema:CreativeWork
210 https://doi.org/10.1016/0003-2697(76)90527-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025529346
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1016/0006-291x(91)90511-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040343100
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1016/0092-8674(89)90292-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032801067
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1016/0092-8674(93)80055-j schema:sameAs https://app.dimensions.ai/details/publication/pub.1004344510
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1016/0167-5699(92)90149-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038923122
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1083/jcb.112.4.749 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009351186
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1084/jem.124.4.733 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019815261
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1084/jem.175.4.1147 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026577196
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1084/jem.177.3.667 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023706904
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1097/00041552-199303000-00001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051422642
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1152/ajpheart.1987.253.3.h699 schema:sameAs https://app.dimensions.ai/details/publication/pub.1079861635
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1161/01.str.25.1.202 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002579660
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1172/jci112965 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046377836
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1172/jci113407 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008248286
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1172/jci114111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026924458
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1172/jci114175 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026244285
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1172/jci116029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013353899
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1172/jci116210 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043231419
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1172/jci116237 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033282179
247 rdf:type schema:CreativeWork
248 https://www.grid.ac/institutes/grid.416060.5 schema:alternateName Monash Medical Centre
249 schema:name Monash University, Department of Medicine, Monash Medical Centre, Clayton, Victoria, Australia.
250 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...