Microbiome and infectivity studies reveal complex polyspecies tree disease in Acute Oak Decline View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-10-13

AUTHORS

Sandra Denman, James Doonan, Emma Ransom-Jones, Martin Broberg, Sarah Plummer, Susan Kirk, Kelly Scarlett, Andrew R Griffiths, Maciej Kaczmarek, Jack Forster, Andrew Peace, Peter N Golyshin, Francis Hassard, Nathan Brown, John G Kenny, James E McDonald

ABSTRACT

Decline-diseases are complex and becoming increasingly problematic to tree health globally. Acute Oak Decline (AOD) is characterized by necrotic stem lesions and galleries of the bark-boring beetle, Agrilus biguttatus, and represents a serious threat to oak. Although multiple novel bacterial species and Agrilus galleries are associated with AOD lesions, the causative agent(s) are unknown. The AOD pathosystem therefore provides an ideal model for a systems-based research approach to address our hypothesis that AOD lesions are caused by a polymicrobial complex. Here we show that three bacterial species, Brenneria goodwinii, Gibbsiella quercinecans and Rahnella victoriana, are consistently abundant in the lesion microbiome and possess virulence genes used by canonical phytopathogens that are expressed in AOD lesions. Individual and polyspecies inoculations on oak logs and trees demonstrated that B. goodwinii and G. quercinecans cause tissue necrosis and, in combination with A. biguttatus, produce the diagnostic symptoms of AOD. We have proved a polybacterial cause of AOD lesions, providing new insights into polymicrobial interactions and tree disease. This work presents a novel conceptual and methodological template for adapting Koch's postulates to address the role of microbial communities in disease. More... »

PAGES

386-399

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/ismej.2017.170

DOI

http://dx.doi.org/10.1038/ismej.2017.170

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1092193047

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29028005


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29 schema:description Decline-diseases are complex and becoming increasingly problematic to tree health globally. Acute Oak Decline (AOD) is characterized by necrotic stem lesions and galleries of the bark-boring beetle, Agrilus biguttatus, and represents a serious threat to oak. Although multiple novel bacterial species and Agrilus galleries are associated with AOD lesions, the causative agent(s) are unknown. The AOD pathosystem therefore provides an ideal model for a systems-based research approach to address our hypothesis that AOD lesions are caused by a polymicrobial complex. Here we show that three bacterial species, Brenneria goodwinii, Gibbsiella quercinecans and Rahnella victoriana, are consistently abundant in the lesion microbiome and possess virulence genes used by canonical phytopathogens that are expressed in AOD lesions. Individual and polyspecies inoculations on oak logs and trees demonstrated that B. goodwinii and G. quercinecans cause tissue necrosis and, in combination with A. biguttatus, produce the diagnostic symptoms of AOD. We have proved a polybacterial cause of AOD lesions, providing new insights into polymicrobial interactions and tree disease. This work presents a novel conceptual and methodological template for adapting Koch's postulates to address the role of microbial communities in disease.
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37 AOD pathosystem
38 Acute Oak Decline
39 Agrilus biguttatus
40 Agrilus galleries
41 Brenneria goodwinii
42 Gibbsiella quercinecans
43 Koch's postulates
44 Rahnella victoriana
45 approach
46 bacterial species
47 bark-boring beetle
48 beetles
49 biguttatus
50 canonical phytopathogens
51 cause
52 combination
53 community
54 complex polyspecies tree disease
55 complexes
56 decline
57 diagnostic symptoms
58 disease
59 galleries
60 genes
61 goodwinii
62 health
63 hypothesis
64 ideal model
65 infectivity studies
66 inoculation
67 insights
68 interaction
69 lesion microbiome
70 lesions
71 logs
72 methodological template
73 microbial communities
74 microbiome
75 model
76 multiple novel bacterial species
77 necrosis
78 necrotic stem lesions
79 new insights
80 novel bacterial species
81 oak
82 oak decline
83 oak logs
84 pathosystem
85 phytopathogens
86 polybacterial cause
87 polymicrobial complex
88 polymicrobial interactions
89 polyspecies inoculations
90 polyspecies tree disease
91 postulates
92 quercinecans
93 research approach
94 role
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96 species
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