High molecular weight dissolved organic matter enrichment selects for methylotrophs in dilution to extinction cultures View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2015-05-15

AUTHORS

Oscar A Sosa, Scott M Gifford, Daniel J Repeta, Edward F DeLong

ABSTRACT

The role of bacterioplankton in the cycling of marine dissolved organic matter (DOM) is central to the carbon and energy balance in the ocean, yet there are few model organisms available to investigate the genes, metabolic pathways, and biochemical mechanisms involved in the degradation of this globally important carbon pool. To obtain microbial isolates capable of degrading semi-labile DOM for growth, we conducted dilution to extinction cultivation experiments using seawater enriched with high molecular weight (HMW) DOM. In total, 93 isolates were obtained. Amendments using HMW DOM to increase the dissolved organic carbon concentration 4x (280 μM) or 10x (700 μM) the ocean surface water concentrations yielded positive growth in 4-6% of replicate dilutions, whereas <1% scored positive for growth in non-DOM-amended controls. The majority (71%) of isolates displayed a distinct increase in cell yields when grown in increasing concentrations of HMW DOM. Whole-genome sequencing was used to screen the culture collection for purity and to determine the phylogenetic identity of the isolates. Eleven percent of the isolates belonged to the gammaproteobacteria including Alteromonadales (the SAR92 clade) and Vibrio. Surprisingly, 85% of isolates belonged to the methylotrophic OM43 clade of betaproteobacteria, bacteria thought to metabolically specialize in degrading C1 compounds. Growth of these isolates on methanol confirmed their methylotrophic phenotype. Our results indicate that dilution to extinction cultivation enriched with natural sources of organic substrates has a potential to reveal the previously unsuspected relationships between naturally occurring organic nutrients and the microorganisms that consume them. More... »

PAGES

2725-2739

References to SciGraph publications

  • 2010-11-11. Environmental proteomics of microbial plankton in a highly productive coastal upwelling system in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2007-07-05. Improvements of high-throughput culturing yielded novel SAR11 strains and other abundant marine bacteria from the Oregon coast and the Bermuda Atlantic Time Series study site in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2012-03-03. Genome sequence of strain HIMB624, a cultured representative from the OM43 clade of marine Betaproteobacteria in ENVIRONMENTAL MICROBIOME
  • 2014-04-29. Discovery of a SAR11 growth requirement for thiamin’s pyrimidine precursor and its distribution in the Sargasso Sea in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2012-08-09. Isolation of an aerobic sulfur oxidizer from the SUP05/Arctic96BD-19 clade in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2004-10-14. Phylogenetic Screening of Ribosomal RNA Gene-Containing Clones in Bacterial Artificial Chromosome (BAC) Libraries from Different Depths in Monterey Bay in MICROBIAL ECOLOGY
  • 1998-06. Marine phosphorus is selectively remineralized in NATURE
  • 2002-08. Cultivation of the ubiquitous SAR11 marine bacterioplankton clade in NATURE
  • 1997-05. A major biopolymeric component to dissolved organic carbon in surface sea water in NATURE
  • 1987-11. Radiocarbon in dissolved organic matter in the central North Pacific Ocean in NATURE
  • 1997-11. Chemical composition of dissolved organic nitrogen in the ocean in NATURE
  • 2007-07-11. Dilution-to-Extinction Culturing of Psychrotolerant Planktonic Bacteria from Permanently Ice-covered Lakes in the McMurdo Dry Valleys, Antarctica in MICROBIAL ECOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/ismej.2015.68

    DOI

    http://dx.doi.org/10.1038/ismej.2015.68

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1003291908

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/25978545


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0605", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Microbiology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Autotrophic Processes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bacteria", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Carbon", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Culture Media", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Methanol", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Sequence Data", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Weight", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Organic Chemicals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Phylogeny", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Seawater", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA, USA.", 
              "id": "http://www.grid.ac/institutes/grid.56466.37", 
              "name": [
                "Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, HI, USA.", 
                "Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.", 
                "Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sosa", 
            "givenName": "Oscar A", 
            "id": "sg:person.01015123407.22", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01015123407.22"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.", 
              "id": "http://www.grid.ac/institutes/grid.116068.8", 
              "name": [
                "Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Gifford", 
            "givenName": "Scott M", 
            "id": "sg:person.01230344374.63", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230344374.63"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, MA, USA.", 
              "id": "http://www.grid.ac/institutes/grid.56466.37", 
              "name": [
                "Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, MA, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Repeta", 
            "givenName": "Daniel J", 
            "id": "sg:person.0764556400.47", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0764556400.47"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.", 
              "id": "http://www.grid.ac/institutes/grid.116068.8", 
              "name": [
                "Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, HI, USA.", 
                "Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA."
              ], 
              "type": "Organization"
            }, 
            "familyName": "DeLong", 
            "givenName": "Edward F", 
            "id": "sg:person.01316715301.06", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01316715301.06"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/330246a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039401049", 
              "https://doi.org/10.1038/330246a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.4056/sigs.2305090", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039585745", 
              "https://doi.org/10.4056/sigs.2305090"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ismej.2014.61", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049962288", 
              "https://doi.org/10.1038/ismej.2014.61"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/36535", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009013005", 
              "https://doi.org/10.1038/36535"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nature00917", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046723721", 
              "https://doi.org/10.1038/nature00917"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00248-007-9284-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001635347", 
              "https://doi.org/10.1007/s00248-007-9284-4"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ismej.2012.78", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014417998", 
              "https://doi.org/10.1038/ismej.2012.78"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/387166a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021159652", 
              "https://doi.org/10.1038/387166a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/30881", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026768047", 
              "https://doi.org/10.1038/30881"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00248-004-0213-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019073556", 
              "https://doi.org/10.1007/s00248-004-0213-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ismej.2010.168", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022189587", 
              "https://doi.org/10.1038/ismej.2010.168"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/ismej.2007.49", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009648090", 
              "https://doi.org/10.1038/ismej.2007.49"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2015-05-15", 
        "datePublishedReg": "2015-05-15", 
        "description": "The role of bacterioplankton in the cycling of marine dissolved organic matter (DOM) is central to the carbon and energy balance in the ocean, yet there are few model organisms available to investigate the genes, metabolic pathways, and biochemical mechanisms involved in the degradation of this globally important carbon pool. To obtain microbial isolates capable of degrading semi-labile DOM for growth, we conducted dilution to extinction cultivation experiments using seawater enriched with high molecular weight (HMW) DOM. In total, 93 isolates were obtained. Amendments using HMW DOM to increase the dissolved organic carbon concentration 4x (280\u2009\u03bcM) or 10x (700\u2009\u03bcM) the ocean surface water concentrations yielded positive growth in 4-6% of replicate dilutions, whereas <1% scored positive for growth in non-DOM-amended controls. The majority (71%) of isolates displayed a distinct increase in cell yields when grown in increasing concentrations of HMW DOM. Whole-genome sequencing was used to screen the culture collection for purity and to determine the phylogenetic identity of the isolates. Eleven percent of the isolates belonged to the gammaproteobacteria including Alteromonadales (the SAR92 clade) and Vibrio. Surprisingly, 85% of isolates belonged to the methylotrophic OM43 clade of betaproteobacteria, bacteria thought to metabolically specialize in degrading C1 compounds. Growth of these isolates on methanol confirmed their methylotrophic phenotype. Our results indicate that dilution to extinction cultivation enriched with natural sources of organic substrates has a potential to reveal the previously unsuspected relationships between naturally occurring organic nutrients and the microorganisms that consume them.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/ismej.2015.68", 
        "inLanguage": "en", 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.3050729", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1038436", 
            "issn": [
              "1751-7362", 
              "1751-7370"
            ], 
            "name": "The ISME Journal: Multidisciplinary Journal of Microbial Ecology", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "12", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "9"
          }
        ], 
        "keywords": [
          "HMW DOM", 
          "role of bacterioplankton", 
          "OM43 clade", 
          "whole-genome sequencing", 
          "extinction cultivation", 
          "model organisms", 
          "phylogenetic identity", 
          "marine dissolved organic matter", 
          "extinction cultures", 
          "C1 compounds", 
          "metabolic pathways", 
          "semi-labile dissolved organic matter", 
          "biochemical mechanisms", 
          "microbial isolates", 
          "Culture Collection", 
          "organic nutrients", 
          "cultivation experiments", 
          "dissolved organic matter", 
          "majority of isolates", 
          "unsuspected relationships", 
          "isolates", 
          "cell yield", 
          "carbon pools", 
          "high molecular weight (HMW) DOM", 
          "important carbon pool", 
          "Alteromonadales", 
          "growth", 
          "clade", 
          "organic substrates", 
          "Gammaproteobacteria", 
          "Betaproteobacteria", 
          "methylotrophs", 
          "bacterioplankton", 
          "molecular weight", 
          "genes", 
          "organisms", 
          "sequencing", 
          "Vibrio", 
          "phenotype", 
          "microorganisms", 
          "bacteria", 
          "pathway", 
          "natural sources", 
          "positive growth", 
          "nutrients", 
          "organic matter", 
          "pool", 
          "distinct increase", 
          "cultivation", 
          "high molecular weight", 
          "cycling", 
          "degradation", 
          "mechanism", 
          "role", 
          "substrate", 
          "identity", 
          "surface water concentrations", 
          "selects", 
          "culture", 
          "seawater", 
          "Ocean", 
          "yield", 
          "energy balance", 
          "concentration", 
          "dilution", 
          "carbon", 
          "compounds", 
          "amendments", 
          "potential", 
          "balance", 
          "majority", 
          "collection", 
          "experiments", 
          "control", 
          "water concentration", 
          "increase", 
          "relationship", 
          "weight", 
          "source", 
          "results", 
          "purity", 
          "matter", 
          "methanol", 
          "percent", 
          "extinction cultivation experiments", 
          "molecular weight (HMW) DOM", 
          "weight (HMW) DOM", 
          "dissolved organic carbon concentration 4x", 
          "organic carbon concentration 4x", 
          "carbon concentration 4x", 
          "concentration 4x", 
          "ocean surface water concentrations", 
          "replicate dilutions", 
          "methylotrophic OM43 clade", 
          "methylotrophic phenotype", 
          "organic matter enrichment selects", 
          "matter enrichment selects", 
          "enrichment selects"
        ], 
        "name": "High molecular weight dissolved organic matter enrichment selects for methylotrophs in dilution to extinction cultures", 
        "pagination": "2725-2739", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1003291908"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/ismej.2015.68"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "25978545"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/ismej.2015.68", 
          "https://app.dimensions.ai/details/publication/pub.1003291908"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-01-01T18:36", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_657.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/ismej.2015.68"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/ismej.2015.68'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/ismej.2015.68'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/ismej.2015.68'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/ismej.2015.68'


     

    This table displays all metadata directly associated to this object as RDF triples.

    279 TRIPLES      22 PREDICATES      146 URIs      126 LITERALS      17 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/ismej.2015.68 schema:about N017378c2b81a44d38b9a8941833483d5
    2 N0dd1d8e77280412ebf6238cc786fec02
    3 N0ffbe21ea12f497eabc0fd984e047e1e
    4 N2cf33080fe6d419b96dad6a00142c25c
    5 N58b8f0ddb1034301be254206532c509e
    6 N7d75fe6b3af4424fa7fb5a2f42e8ab5c
    7 N8ac08c9e10bd4c33aa20600a393ad21d
    8 Nbca007b999b946cda3195363eb157b1a
    9 Nddc96cffb09d474b8c2ec6246fed6b11
    10 Nee5451336b77492fbe700fea95f8b14c
    11 anzsrc-for:06
    12 anzsrc-for:0605
    13 schema:author N4ad2789d5c8b488d9721786d414cde86
    14 schema:citation sg:pub.10.1007/s00248-004-0213-5
    15 sg:pub.10.1007/s00248-007-9284-4
    16 sg:pub.10.1038/30881
    17 sg:pub.10.1038/330246a0
    18 sg:pub.10.1038/36535
    19 sg:pub.10.1038/387166a0
    20 sg:pub.10.1038/ismej.2007.49
    21 sg:pub.10.1038/ismej.2010.168
    22 sg:pub.10.1038/ismej.2012.78
    23 sg:pub.10.1038/ismej.2014.61
    24 sg:pub.10.1038/nature00917
    25 sg:pub.10.4056/sigs.2305090
    26 schema:datePublished 2015-05-15
    27 schema:datePublishedReg 2015-05-15
    28 schema:description The role of bacterioplankton in the cycling of marine dissolved organic matter (DOM) is central to the carbon and energy balance in the ocean, yet there are few model organisms available to investigate the genes, metabolic pathways, and biochemical mechanisms involved in the degradation of this globally important carbon pool. To obtain microbial isolates capable of degrading semi-labile DOM for growth, we conducted dilution to extinction cultivation experiments using seawater enriched with high molecular weight (HMW) DOM. In total, 93 isolates were obtained. Amendments using HMW DOM to increase the dissolved organic carbon concentration 4x (280 μM) or 10x (700 μM) the ocean surface water concentrations yielded positive growth in 4-6% of replicate dilutions, whereas <1% scored positive for growth in non-DOM-amended controls. The majority (71%) of isolates displayed a distinct increase in cell yields when grown in increasing concentrations of HMW DOM. Whole-genome sequencing was used to screen the culture collection for purity and to determine the phylogenetic identity of the isolates. Eleven percent of the isolates belonged to the gammaproteobacteria including Alteromonadales (the SAR92 clade) and Vibrio. Surprisingly, 85% of isolates belonged to the methylotrophic OM43 clade of betaproteobacteria, bacteria thought to metabolically specialize in degrading C1 compounds. Growth of these isolates on methanol confirmed their methylotrophic phenotype. Our results indicate that dilution to extinction cultivation enriched with natural sources of organic substrates has a potential to reveal the previously unsuspected relationships between naturally occurring organic nutrients and the microorganisms that consume them.
    29 schema:genre article
    30 schema:inLanguage en
    31 schema:isAccessibleForFree true
    32 schema:isPartOf N1a3ed98338684a4ea411b7a3e94a3522
    33 N97de312526d64a45a958119bfdfae656
    34 sg:journal.1038436
    35 schema:keywords Alteromonadales
    36 Betaproteobacteria
    37 C1 compounds
    38 Culture Collection
    39 Gammaproteobacteria
    40 HMW DOM
    41 OM43 clade
    42 Ocean
    43 Vibrio
    44 amendments
    45 bacteria
    46 bacterioplankton
    47 balance
    48 biochemical mechanisms
    49 carbon
    50 carbon concentration 4x
    51 carbon pools
    52 cell yield
    53 clade
    54 collection
    55 compounds
    56 concentration
    57 concentration 4x
    58 control
    59 cultivation
    60 cultivation experiments
    61 culture
    62 cycling
    63 degradation
    64 dilution
    65 dissolved organic carbon concentration 4x
    66 dissolved organic matter
    67 distinct increase
    68 energy balance
    69 enrichment selects
    70 experiments
    71 extinction cultivation
    72 extinction cultivation experiments
    73 extinction cultures
    74 genes
    75 growth
    76 high molecular weight
    77 high molecular weight (HMW) DOM
    78 identity
    79 important carbon pool
    80 increase
    81 isolates
    82 majority
    83 majority of isolates
    84 marine dissolved organic matter
    85 matter
    86 matter enrichment selects
    87 mechanism
    88 metabolic pathways
    89 methanol
    90 methylotrophic OM43 clade
    91 methylotrophic phenotype
    92 methylotrophs
    93 microbial isolates
    94 microorganisms
    95 model organisms
    96 molecular weight
    97 molecular weight (HMW) DOM
    98 natural sources
    99 nutrients
    100 ocean surface water concentrations
    101 organic carbon concentration 4x
    102 organic matter
    103 organic matter enrichment selects
    104 organic nutrients
    105 organic substrates
    106 organisms
    107 pathway
    108 percent
    109 phenotype
    110 phylogenetic identity
    111 pool
    112 positive growth
    113 potential
    114 purity
    115 relationship
    116 replicate dilutions
    117 results
    118 role
    119 role of bacterioplankton
    120 seawater
    121 selects
    122 semi-labile dissolved organic matter
    123 sequencing
    124 source
    125 substrate
    126 surface water concentrations
    127 unsuspected relationships
    128 water concentration
    129 weight
    130 weight (HMW) DOM
    131 whole-genome sequencing
    132 yield
    133 schema:name High molecular weight dissolved organic matter enrichment selects for methylotrophs in dilution to extinction cultures
    134 schema:pagination 2725-2739
    135 schema:productId N1b0b91f9338f4d87ba92986fad554419
    136 N28b82f9810ba4de3b6d0a1c0906dfb4b
    137 N34fc95e519c64cf6bee3e5de30a17305
    138 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003291908
    139 https://doi.org/10.1038/ismej.2015.68
    140 schema:sdDatePublished 2022-01-01T18:36
    141 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    142 schema:sdPublisher Nf69bb1c864204a1792f3eb9bbf0b477f
    143 schema:url https://doi.org/10.1038/ismej.2015.68
    144 sgo:license sg:explorer/license/
    145 sgo:sdDataset articles
    146 rdf:type schema:ScholarlyArticle
    147 N017378c2b81a44d38b9a8941833483d5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    148 schema:name Organic Chemicals
    149 rdf:type schema:DefinedTerm
    150 N0dd1d8e77280412ebf6238cc786fec02 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    151 schema:name Methanol
    152 rdf:type schema:DefinedTerm
    153 N0ffbe21ea12f497eabc0fd984e047e1e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    154 schema:name Phylogeny
    155 rdf:type schema:DefinedTerm
    156 N1a3ed98338684a4ea411b7a3e94a3522 schema:issueNumber 12
    157 rdf:type schema:PublicationIssue
    158 N1b0b91f9338f4d87ba92986fad554419 schema:name dimensions_id
    159 schema:value pub.1003291908
    160 rdf:type schema:PropertyValue
    161 N1b8314b861e94e1ea7aca57737872569 rdf:first sg:person.0764556400.47
    162 rdf:rest N4293edfaa63140d08f14927d209ce95b
    163 N28b82f9810ba4de3b6d0a1c0906dfb4b schema:name doi
    164 schema:value 10.1038/ismej.2015.68
    165 rdf:type schema:PropertyValue
    166 N2cf33080fe6d419b96dad6a00142c25c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    167 schema:name Carbon
    168 rdf:type schema:DefinedTerm
    169 N34fc95e519c64cf6bee3e5de30a17305 schema:name pubmed_id
    170 schema:value 25978545
    171 rdf:type schema:PropertyValue
    172 N4293edfaa63140d08f14927d209ce95b rdf:first sg:person.01316715301.06
    173 rdf:rest rdf:nil
    174 N4ad2789d5c8b488d9721786d414cde86 rdf:first sg:person.01015123407.22
    175 rdf:rest Nd07b9bfa7c4148d2917171c682ca60c8
    176 N58b8f0ddb1034301be254206532c509e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    177 schema:name Culture Media
    178 rdf:type schema:DefinedTerm
    179 N7d75fe6b3af4424fa7fb5a2f42e8ab5c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    180 schema:name Molecular Weight
    181 rdf:type schema:DefinedTerm
    182 N8ac08c9e10bd4c33aa20600a393ad21d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    183 schema:name Autotrophic Processes
    184 rdf:type schema:DefinedTerm
    185 N97de312526d64a45a958119bfdfae656 schema:volumeNumber 9
    186 rdf:type schema:PublicationVolume
    187 Nbca007b999b946cda3195363eb157b1a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    188 schema:name Bacteria
    189 rdf:type schema:DefinedTerm
    190 Nd07b9bfa7c4148d2917171c682ca60c8 rdf:first sg:person.01230344374.63
    191 rdf:rest N1b8314b861e94e1ea7aca57737872569
    192 Nddc96cffb09d474b8c2ec6246fed6b11 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    193 schema:name Seawater
    194 rdf:type schema:DefinedTerm
    195 Nee5451336b77492fbe700fea95f8b14c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    196 schema:name Molecular Sequence Data
    197 rdf:type schema:DefinedTerm
    198 Nf69bb1c864204a1792f3eb9bbf0b477f schema:name Springer Nature - SN SciGraph project
    199 rdf:type schema:Organization
    200 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    201 schema:name Biological Sciences
    202 rdf:type schema:DefinedTerm
    203 anzsrc-for:0605 schema:inDefinedTermSet anzsrc-for:
    204 schema:name Microbiology
    205 rdf:type schema:DefinedTerm
    206 sg:grant.3050729 http://pending.schema.org/fundedItem sg:pub.10.1038/ismej.2015.68
    207 rdf:type schema:MonetaryGrant
    208 sg:journal.1038436 schema:issn 1751-7362
    209 1751-7370
    210 schema:name The ISME Journal: Multidisciplinary Journal of Microbial Ecology
    211 schema:publisher Springer Nature
    212 rdf:type schema:Periodical
    213 sg:person.01015123407.22 schema:affiliation grid-institutes:grid.56466.37
    214 schema:familyName Sosa
    215 schema:givenName Oscar A
    216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01015123407.22
    217 rdf:type schema:Person
    218 sg:person.01230344374.63 schema:affiliation grid-institutes:grid.116068.8
    219 schema:familyName Gifford
    220 schema:givenName Scott M
    221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230344374.63
    222 rdf:type schema:Person
    223 sg:person.01316715301.06 schema:affiliation grid-institutes:grid.116068.8
    224 schema:familyName DeLong
    225 schema:givenName Edward F
    226 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01316715301.06
    227 rdf:type schema:Person
    228 sg:person.0764556400.47 schema:affiliation grid-institutes:grid.56466.37
    229 schema:familyName Repeta
    230 schema:givenName Daniel J
    231 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0764556400.47
    232 rdf:type schema:Person
    233 sg:pub.10.1007/s00248-004-0213-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019073556
    234 https://doi.org/10.1007/s00248-004-0213-5
    235 rdf:type schema:CreativeWork
    236 sg:pub.10.1007/s00248-007-9284-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001635347
    237 https://doi.org/10.1007/s00248-007-9284-4
    238 rdf:type schema:CreativeWork
    239 sg:pub.10.1038/30881 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026768047
    240 https://doi.org/10.1038/30881
    241 rdf:type schema:CreativeWork
    242 sg:pub.10.1038/330246a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039401049
    243 https://doi.org/10.1038/330246a0
    244 rdf:type schema:CreativeWork
    245 sg:pub.10.1038/36535 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009013005
    246 https://doi.org/10.1038/36535
    247 rdf:type schema:CreativeWork
    248 sg:pub.10.1038/387166a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021159652
    249 https://doi.org/10.1038/387166a0
    250 rdf:type schema:CreativeWork
    251 sg:pub.10.1038/ismej.2007.49 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009648090
    252 https://doi.org/10.1038/ismej.2007.49
    253 rdf:type schema:CreativeWork
    254 sg:pub.10.1038/ismej.2010.168 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022189587
    255 https://doi.org/10.1038/ismej.2010.168
    256 rdf:type schema:CreativeWork
    257 sg:pub.10.1038/ismej.2012.78 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014417998
    258 https://doi.org/10.1038/ismej.2012.78
    259 rdf:type schema:CreativeWork
    260 sg:pub.10.1038/ismej.2014.61 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049962288
    261 https://doi.org/10.1038/ismej.2014.61
    262 rdf:type schema:CreativeWork
    263 sg:pub.10.1038/nature00917 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046723721
    264 https://doi.org/10.1038/nature00917
    265 rdf:type schema:CreativeWork
    266 sg:pub.10.4056/sigs.2305090 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039585745
    267 https://doi.org/10.4056/sigs.2305090
    268 rdf:type schema:CreativeWork
    269 grid-institutes:grid.116068.8 schema:alternateName Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
    270 schema:name Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, HI, USA.
    271 Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
    272 rdf:type schema:Organization
    273 grid-institutes:grid.56466.37 schema:alternateName Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA, USA.
    274 Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, MA, USA.
    275 schema:name Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA, USA.
    276 Center for Microbial Oceanography: Research and Education, University of Hawaii, Honolulu, HI, USA.
    277 Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
    278 Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, MA, USA.
    279 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...