Aerobic nitrous oxide production through N-nitrosating hybrid formation in ammonia-oxidizing archaea View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2014-01-09

AUTHORS

Michaela Stieglmeier, Maria Mooshammer, Barbara Kitzler, Wolfgang Wanek, Sophie Zechmeister-Boltenstern, Andreas Richter, Christa Schleper

ABSTRACT

Soil emissions are largely responsible for the increase of the potent greenhouse gas nitrous oxide (N2O) in the atmosphere and are generally attributed to the activity of nitrifying and denitrifying bacteria. However, the contribution of the recently discovered ammonia-oxidizing archaea (AOA) to N2O production from soil is unclear as is the mechanism by which they produce it. Here we investigate the potential of Nitrososphaera viennensis, the first pure culture of AOA from soil, to produce N2O and compare its activity with that of a marine AOA and an ammonia-oxidizing bacterium (AOB) from soil. N. viennensis produced N2O at a maximum yield of 0.09% N2O per molecule of nitrite under oxic growth conditions. N2O production rates of 4.6±0.6 amol N2O cell−1 h−1 and nitrification rates of 2.6±0.5 fmol NO2− cell−1 h−1 were in the same range as those of the AOB Nitrosospira multiformis and the marine AOA Nitrosopumilus maritimus grown under comparable conditions. In contrast to AOB, however, N2O production of the two archaeal strains did not increase when the oxygen concentration was reduced, suggesting that they are not capable of denitrification. In 15N-labeling experiments we provide evidence that both ammonium and nitrite contribute equally via hybrid N2O formation to the N2O produced by N. viennensis under all conditions tested. Our results suggest that archaea may contribute to N2O production in terrestrial ecosystems, however, they are not capable of nitrifier-denitrification and thus do not produce increasing amounts of the greenhouse gas when oxygen becomes limiting. More... »

PAGES

1135-1146

References to SciGraph publications

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  • 2009-09-30. Ammonia oxidation kinetics determine niche separation of nitrifying Archaea and Bacteria in NATURE
  • 2011-01-13. Ammonia concentration determines differential growth of ammonia-oxidising archaea and bacteria in soil microcosms in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 1988-03. Short-term assay of soil urease activity using colorimetric determination of ammonium in BIOLOGY AND FERTILITY OF SOILS
  • 2008-07-24. Transcription of nitrification genes by the methane-oxidizing bacterium, Methylococcus capsulatus strain Bath in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2004-10. Contribution of nitrification and denitrification to N2O production in peat, clay and loamy sand soils under different soil moisture conditions in NUTRIENT CYCLING IN AGROECOSYSTEMS
  • 2005-09. Isolation of an autotrophic ammonia-oxidizing marine archaeon in NATURE
  • 2010-11-18. Metatranscriptomic analysis of ammonia-oxidizing organisms in an estuarine bacterioplankton assemblage in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2006-08. Archaea predominate among ammonia-oxidizing prokaryotes in soils in NATURE
  • 2008-01-19. Evidence that Ammonia-Oxidizing Archaea are More Abundant than Ammonia-Oxidizing Bacteria in Semiarid Soils of Northern Arizona, USA in MICROBIAL ECOLOGY
  • 1961-12. Growth of Nitrosomonas europaea in batch and continuous culture in ARCHIVES OF MICROBIOLOGY
  • 1996-06. Contributions from different microbial processes to N2O emission from soil under different moisture regimes in BIOLOGY AND FERTILITY OF SOILS
  • 2001-01. Archaeal dominance in the mesopelagic zone of the Pacific Ocean in NATURE
  • 1999-11. Immediate and adaptational temperature effects on nitric oxide production and nitrous oxide release from nitrification and denitrification in two soils in BIOLOGY AND FERTILITY OF SOILS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/ismej.2013.220

    DOI

    http://dx.doi.org/10.1038/ismej.2013.220

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1035581095

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24401864


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    80 nitrification rates
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    82 nitrous oxide
    83 nitrous oxide production
    84 oxic growth conditions
    85 oxide
    86 oxide production
    87 oxygen
    88 oxygen concentration
    89 potent greenhouse gas nitrous oxide
    90 potential
    91 production
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    93 pure culture
    94 range
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