Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2012-08

AUTHORS

J Gregory Caporaso, Christian L Lauber, William A Walters, Donna Berg-Lyons, James Huntley, Noah Fierer, Sarah M Owens, Jason Betley, Louise Fraser, Markus Bauer, Niall Gormley, Jack A Gilbert, Geoff Smith, Rob Knight

ABSTRACT

DNA sequencing continues to decrease in cost with the Illumina HiSeq2000 generating up to 600 Gb of paired-end 100 base reads in a ten-day run. Here we present a protocol for community amplicon sequencing on the HiSeq2000 and MiSeq Illumina platforms, and apply that protocol to sequence 24 microbial communities from host-associated and free-living environments. A critical question as more sequencing platforms become available is whether biological conclusions derived on one platform are consistent with what would be derived on a different platform. We show that the protocol developed for these instruments successfully recaptures known biological results, and additionally that biological conclusions are consistent across sequencing platforms (the HiSeq2000 versus the MiSeq) and across the sequenced regions of amplicons. More... »

PAGES

1621

Journal

TITLE

The ISME Journal

ISSUE

8

VOLUME

6

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/ismej.2012.8

DOI

http://dx.doi.org/10.1038/ismej.2012.8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1038992953

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22402401


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